EC 2.7.7.4: sulfate adenylyltransferase

TransferasesOpen in Browse

CatLog read 159 records for EC 2.7.7.4 from 6 papers, for 5 organisms. Names in these papers: ATP sulfurylase, ATP sulfurylase isoform 1 (GmATPS), PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain, ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit) and 5 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 44 records for EC 2.7.7.4 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.7.7.4 in Browse.

Papers

Papers with records for EC 2.7.7.4
Paper	Organisms	Records
Kinetic and stability properties of Penicillium chrysogenum ATP sulfurylase missing the C-terminal regulatory domain	Penicillium chrysogenum	50
Structure and mechanism of soybean ATP sulfurylase and the committed step in plant sulfur assimilation	Glycine max	42
Human 3'-phosphoadenosine 5'-phosphosulfate synthetase (isoform 1, brain): kinetic properties of the adenosine triphosphate sulfurylase and adenosine 5'-phosphosulfate kinase domains	Homo sapiens	26
Allosteric and catalytic functions of the PPi-binding motif in the ATP sulfurylase-GTPase system	Escherichia coli	18
ATP sulfurylase from higher plants: kinetic and structural characterization of the chloroplast and cytosol enzymes from spinach leaf	Spinacia oleracea	17
Characterization and expression of human bifunctional 3'-phosphoadenosine 5'-phosphosulphate synthase isoforms	Homo sapiens	6

Records

Records for EC 2.7.7.4 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
ATP sulfurylase	Penicillium chrysogenum	adenosine 5'-phosphosulfate	—	4 × 10⁻⁴	—	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase, adenosine 5'-phosphosulfate
ATP sulfurylase	Penicillium chrysogenum	chromate	3.4	0.006	570 calc.	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase, chromate
ATP sulfurylase	Penicillium chrysogenum	fluorophosphate	2.3	0.11	21 calc.	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase, fluorophosphate
ATP sulfurylase	Penicillium chrysogenum	hydrogen arsenatecoupled assay: myokinase	11.5	8	1.4 calc.	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase, hydrogen arsenate
ATP sulfurylase	Penicillium chrysogenum	hydrogen arsenatecoupled assay: APS kinase; Ap5A 135 µM	0.5	3.5	0.14 calc.	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase, hydrogen arsenate
ATP sulfurylase	Penicillium chrysogenum	hydrogen phosphate	0.4	—	—	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase, hydrogen phosphate
ATP sulfurylase	Penicillium chrysogenum	magnesium adenosine triphosphate5 mM excess MgCl2 over total ATP; coupled assay: APS kinase	—	0.21	—	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase, magnesium adenosine triphosphate
ATP sulfurylase	Penicillium chrysogenum	magnesium adenosine triphosphate5 mM excess MgCl2 over total ATP; molybdolysis reaction	—	0.027	—	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase, magnesium adenosine triphosphate
ATP sulfurylase	Penicillium chrysogenum	magnesium adenosine triphosphateTable II oxyanion series; coupled assay: APS kinase	—	0.24	—	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase, magnesium adenosine triphosphate
ATP sulfurylase	Penicillium chrysogenum	magnesium adenosine triphosphateTable II oxyanion series; coupled assay: APS kinase	—	0.09	—	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase, magnesium adenosine triphosphate
ATP sulfurylase	Penicillium chrysogenum	magnesium adenosine triphosphateTable II oxyanion series; coupled assay: APS kinase	—	0.06	—	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase, magnesium adenosine triphosphate
ATP sulfurylase	Penicillium chrysogenum	magnesium adenosine triphosphateTable II oxyanion series; coupled assay: myokinase; Table II	—	0.06	—	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase, magnesium adenosine triphosphate
ATP sulfurylase	Penicillium chrysogenum	magnesium adenosine triphosphateTable II oxyanion series; cosubstrate phosphate (20 mM)	—	3	—	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase, magnesium adenosine triphosphate
ATP sulfurylase	Penicillium chrysogenum	magnesium adenosine triphosphateTable II oxyanion series; coupled assay: myokinase; Table II	—	0.98	—	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase, magnesium adenosine triphosphate
ATP sulfurylase	Penicillium chrysogenum	magnesium adenosine triphosphateTable II oxyanion series; coupled assay: APS kinase	—	1.25	—	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase, magnesium adenosine triphosphate
ATP sulfurylase	Penicillium chrysogenum	magnesium adenosine triphosphateTable II oxyanion series; coupled assay: myokinase; Table II	—	0.01	—	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase, magnesium adenosine triphosphate
ATP sulfurylase	Penicillium chrysogenum	magnesium adenosine triphosphateTable II oxyanion series; coupled assay: myokinase; Table II	—	0.07	—	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase, magnesium adenosine triphosphate
ATP sulfurylase	Penicillium chrysogenum	magnesium adenosine triphosphateTable II oxyanion series; coupled assay: myokinase; Table II	—	0.01	—	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase, magnesium adenosine triphosphate
ATP sulfurylase	Penicillium chrysogenum	molybdate5 mM excess MgCl2 over total ATP; molybdolysis reaction	24.4	0.076	320 calc.	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase, molybdate
ATP sulfurylase	Penicillium chrysogenum	molybdateTable II oxyanion series; cosubstrate MgATP (5 mM)	23.9	0.1	240 calc.	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase, molybdate
ATP sulfurylase	Penicillium chrysogenum	pyrophosphate	73.3	0.0092	8.0 × 10³ calc.	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase, pyrophosphate
ATP sulfurylase	Penicillium chrysogenum	selenatecoupled assay: APS kinase; Ap5A 135 µM	2.1	0.06	35 calc.	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase, selenate
ATP sulfurylase	Penicillium chrysogenum	selenatecoupled assay: myokinase	1	0.07	14 calc.	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase, selenate
ATP sulfurylase	Penicillium chrysogenum	sulfate5 mM excess MgCl2 over total ATP	10.8	0.29	37 calc.	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase, sulfate
ATP sulfurylase	Penicillium chrysogenum	sulfateTable II oxyanion series; cosubstrate MgATP (5 mM)	10.2	0.3	34 calc.	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase, sulfate
ATP sulfurylase	Penicillium chrysogenum	tungstate	26.6	0.2	130 calc.	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase, tungstate
ATP sulfurylaseΔ396-573 (C-terminal regulatory domain deleted)	Penicillium chrysogenum	adenosine 5'-phosphosulfate	—	5 × 10⁻⁴	—	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase Δ396-573 (C-terminal regulatory domain deleted), adenosine 5'-phosphosulfate
ATP sulfurylaseΔ396-573 (C-terminal regulatory domain deleted)	Penicillium chrysogenum	chromate	2	0.08	25 calc.	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase Δ396-573 (C-terminal regulatory domain deleted), chromate
ATP sulfurylaseΔ396-573 (C-terminal regulatory domain deleted)	Penicillium chrysogenum	fluorophosphate	0.7	4.6	0.15 calc.	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase Δ396-573 (C-terminal regulatory domain deleted), fluorophosphate
ATP sulfurylaseΔ396-573 (C-terminal regulatory domain deleted)	Penicillium chrysogenum	hydrogen arsenatecoupled assay: myokinase	0.11	5.8	0.019 calc.	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase Δ396-573 (C-terminal regulatory domain deleted), hydrogen arsenate
ATP sulfurylaseΔ396-573 (C-terminal regulatory domain deleted)	Penicillium chrysogenum	hydrogen arsenatecoupled assay: APS kinase; Ap5A 135 µM	0.08	13.5	0.0059 calc.	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase Δ396-573 (C-terminal regulatory domain deleted), hydrogen arsenate
ATP sulfurylaseΔ396-573 (C-terminal regulatory domain deleted)	Penicillium chrysogenum	magnesium adenosine triphosphate5 mM excess MgCl2 over total ATP; coupled assay: APS kinase	—	2.6	—	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase Δ396-573 (C-terminal regulatory domain deleted), magnesium adenosine triphosphate
ATP sulfurylaseΔ396-573 (C-terminal regulatory domain deleted)	Penicillium chrysogenum	magnesium adenosine triphosphate5 mM excess MgCl2 over total ATP; molybdolysis reaction	—	0.27	—	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase Δ396-573 (C-terminal regulatory domain deleted), magnesium adenosine triphosphate
ATP sulfurylaseΔ396-573 (C-terminal regulatory domain deleted)	Penicillium chrysogenum	magnesium adenosine triphosphateTable II oxyanion series; coupled assay: APS kinase	—	1.3	—	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase Δ396-573 (C-terminal regulatory domain deleted), magnesium adenosine triphosphate
ATP sulfurylaseΔ396-573 (C-terminal regulatory domain deleted)	Penicillium chrysogenum	magnesium adenosine triphosphateTable II oxyanion series; coupled assay: APS kinase	—	5.2	—	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase Δ396-573 (C-terminal regulatory domain deleted), magnesium adenosine triphosphate
ATP sulfurylaseΔ396-573 (C-terminal regulatory domain deleted)	Penicillium chrysogenum	magnesium adenosine triphosphateTable II oxyanion series; coupled assay: APS kinase	—	1.8	—	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase Δ396-573 (C-terminal regulatory domain deleted), magnesium adenosine triphosphate
ATP sulfurylaseΔ396-573 (C-terminal regulatory domain deleted)	Penicillium chrysogenum	magnesium adenosine triphosphateTable II oxyanion series; coupled assay: myokinase; Table II	—	2.4	—	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase Δ396-573 (C-terminal regulatory domain deleted), magnesium adenosine triphosphate
ATP sulfurylaseΔ396-573 (C-terminal regulatory domain deleted)	Penicillium chrysogenum	magnesium adenosine triphosphateTable II oxyanion series; coupled assay: myokinase; Table II	—	1.9	—	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase Δ396-573 (C-terminal regulatory domain deleted), magnesium adenosine triphosphate
ATP sulfurylaseΔ396-573 (C-terminal regulatory domain deleted)	Penicillium chrysogenum	magnesium adenosine triphosphateTable II oxyanion series; coupled assay: APS kinase	—	9.3	—	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase Δ396-573 (C-terminal regulatory domain deleted), magnesium adenosine triphosphate
ATP sulfurylaseΔ396-573 (C-terminal regulatory domain deleted)	Penicillium chrysogenum	magnesium adenosine triphosphateTable II oxyanion series; coupled assay: myokinase; Table II	—	0.1	—	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase Δ396-573 (C-terminal regulatory domain deleted), magnesium adenosine triphosphate
ATP sulfurylaseΔ396-573 (C-terminal regulatory domain deleted)	Penicillium chrysogenum	magnesium adenosine triphosphateTable II oxyanion series; coupled assay: myokinase; Table II	—	1	—	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase Δ396-573 (C-terminal regulatory domain deleted), magnesium adenosine triphosphate
ATP sulfurylaseΔ396-573 (C-terminal regulatory domain deleted)	Penicillium chrysogenum	magnesium adenosine triphosphateTable II oxyanion series; coupled assay: myokinase; Table II	—	0.6	—	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase Δ396-573 (C-terminal regulatory domain deleted), magnesium adenosine triphosphate
ATP sulfurylaseΔ396-573 (C-terminal regulatory domain deleted)	Penicillium chrysogenum	molybdate5 mM excess MgCl2 over total ATP; molybdolysis reaction	13.7	0.53	26 calc.	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase Δ396-573 (C-terminal regulatory domain deleted), molybdate
ATP sulfurylaseΔ396-573 (C-terminal regulatory domain deleted)	Penicillium chrysogenum	molybdateTable II oxyanion series; cosubstrate MgATP (5 mM)	16.8	0.6	28 calc.	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase Δ396-573 (C-terminal regulatory domain deleted), molybdate
ATP sulfurylaseΔ396-573 (C-terminal regulatory domain deleted)	Penicillium chrysogenum	pyrophosphate	46.6	0.025	1.9 × 10³ calc.	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase Δ396-573 (C-terminal regulatory domain deleted), pyrophosphate
ATP sulfurylaseΔ396-573 (C-terminal regulatory domain deleted)	Penicillium chrysogenum	selenatecoupled assay: APS kinase; Ap5A 135 µM	1.4	3.2	0.44 calc.	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase Δ396-573 (C-terminal regulatory domain deleted), selenate
ATP sulfurylaseΔ396-573 (C-terminal regulatory domain deleted)	Penicillium chrysogenum	selenatecoupled assay: myokinase	0.9	3.2	0.28 calc.	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase Δ396-573 (C-terminal regulatory domain deleted), selenate
ATP sulfurylaseΔ396-573 (C-terminal regulatory domain deleted)	Penicillium chrysogenum	sulfate5 mM excess MgCl2 over total ATP	1.8	3.6	0.50 calc.	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase Δ396-573 (C-terminal regulatory domain deleted), sulfate
ATP sulfurylaseΔ396-573 (C-terminal regulatory domain deleted)	Penicillium chrysogenum	sulfateTable II oxyanion series; cosubstrate MgATP (5 mM)	1.3	5.3	0.25 calc.	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase Δ396-573 (C-terminal regulatory domain deleted), sulfate
ATP sulfurylaseΔ396-573 (C-terminal regulatory domain deleted)	Penicillium chrysogenum	tungstate	14.1	2	7.0 calc.	pH 8, 30 °C	PMID 14613928	Open record: ATP sulfurylase Δ396-573 (C-terminal regulatory domain deleted), tungstate
ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit)	Escherichia coli	adenosine 5'-phosphosulfate	0.6	0.1	6.0 calc.	pH 8, 25 °C	PMID 14506286	Open record: ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit), adenosine 5'-phosphosulfate
ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit)	Escherichia coli	guanosine 5'-triphosphateMgCl2 = [GTP] + 1.0 mM	2.1	0.051	41 calc.	pH 8, 25 °C	PMID 14506286	Open record: ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit), guanosine 5'-triphosphate
ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit)	Escherichia coli	guanosine 5'-triphosphateMgCl2 = [GTP] + [PPi] + 0.70 mM	0.33	0.009	37 calc.	pH 8, 25 °C	PMID 14506286	Open record: ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit), guanosine 5'-triphosphate
ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit)	Escherichia coli	inorganic pyrophosphate	—	0.052	—	pH 8, 25 °C	PMID 14506286	Open record: ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit), inorganic pyrophosphate
ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit)variant D39A	Escherichia coli	guanosine 5'-triphosphateMgCl2 = [GTP] + 1.0 mM	3.6	0.24	15 calc.	pH 8, 25 °C	PMID 14506286	Open record: ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit) variant D39A, guanosine 5'-triphosphate
ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit)variant D39A	Escherichia coli	guanosine 5'-triphosphateMgCl2 = [GTP] + [PPi] + 0.70 mM	0.24	0.29	0.83 calc.	pH 8, 25 °C	PMID 14506286	Open record: ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit) variant D39A, guanosine 5'-triphosphate
ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit)variant G37A	Escherichia coli	adenosine 5'-phosphosulfate	0.051	0.68	0.075 calc.	pH 8, 25 °C	PMID 14506286	Open record: ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit) variant G37A, adenosine 5'-phosphosulfate
ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit)variant G37A	Escherichia coli	guanosine 5'-triphosphateMgCl2 = [GTP] + 1.0 mM	1.87	0.1	19 calc.	pH 8, 25 °C	PMID 14506286	Open record: ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit) variant G37A, guanosine 5'-triphosphate
ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit)variant G37A	Escherichia coli	guanosine 5'-triphosphateMgCl2 = [GTP] + [PPi] + 0.70 mM	0.035	0.11	0.32 calc.	pH 8, 25 °C	PMID 14506286	Open record: ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit) variant G37A, guanosine 5'-triphosphate
ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit)variant G37A	Escherichia coli	inorganic pyrophosphate	—	8.3	—	pH 8, 25 °C	PMID 14506286	Open record: ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit) variant G37A, inorganic pyrophosphate
ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit)variant S35A	Escherichia coli	adenosine 5'-phosphosulfate	0.85	0.13	6.5 calc.	pH 8, 25 °C	PMID 14506286	Open record: ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit) variant S35A, adenosine 5'-phosphosulfate
ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit)variant S35A	Escherichia coli	guanosine 5'-triphosphateMgCl2 = [GTP] + 1.0 mM	1.5	0.05	30 calc.	pH 8, 25 °C	PMID 14506286	Open record: ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit) variant S35A, guanosine 5'-triphosphate
ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit)variant S35A	Escherichia coli	guanosine 5'-triphosphateMgCl2 = [GTP] + [PPi] + 0.70 mM	0.24	0.085	2.8 calc.	pH 8, 25 °C	PMID 14506286	Open record: ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit) variant S35A, guanosine 5'-triphosphate
ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit)variant S35A	Escherichia coli	inorganic pyrophosphate	—	0.16	—	pH 8, 25 °C	PMID 14506286	Open record: ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit) variant S35A, inorganic pyrophosphate
ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit)variant S40A	Escherichia coli	adenosine 5'-phosphosulfate	3.2	0.49	6.5 calc.	pH 8, 25 °C	PMID 14506286	Open record: ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit) variant S40A, adenosine 5'-phosphosulfate
ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit)variant S40A	Escherichia coli	guanosine 5'-triphosphateMgCl2 = [GTP] + 1.0 mM	1	0.083	12 calc.	pH 8, 25 °C	PMID 14506286	Open record: ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit) variant S40A, guanosine 5'-triphosphate
ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit)variant S40A	Escherichia coli	guanosine 5'-triphosphateMgCl2 = [GTP] + [PPi] + 0.70 mM	0.25	0.17	1.5 calc.	pH 8, 25 °C	PMID 14506286	Open record: ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit) variant S40A, guanosine 5'-triphosphate
ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit)variant S40A	Escherichia coli	inorganic pyrophosphate	—	1.8	—	pH 8, 25 °C	PMID 14506286	Open record: ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit) variant S40A, inorganic pyrophosphate
ATP sulfurylase isoform 1 (GmATPS)	Glycine max	adenosine 5'-phosphosulfate	32	0.0342	936	pH 8, 25 °C	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS), adenosine 5'-phosphosulfate
ATP sulfurylase isoform 1 (GmATPS)	Glycine max	adenosine 5'-triphosphate	0.512	0.165	3.1	pH 8	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS), adenosine 5'-triphosphate
ATP sulfurylase isoform 1 (GmATPS)	Glycine max	pyrophosphate	—	0.0458	699	pH 8, 25 °C	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS), pyrophosphate
ATP sulfurylase isoform 1 (GmATPS)	Glycine max	sulfate	—	0.32	1.6	pH 8	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS), sulfate
ATP sulfurylase isoform 1 (GmATPS)variant F245A	Glycine max	adenosine 5'-phosphosulfate	108	0.0363	2.98 × 10³	pH 8, 25 °C	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS) variant F245A, adenosine 5'-phosphosulfate
ATP sulfurylase isoform 1 (GmATPS)variant F245A	Glycine max	pyrophosphate	—	0.153	706	pH 8, 25 °C	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS) variant F245A, pyrophosphate
ATP sulfurylase isoform 1 (GmATPS)variant F245L	Glycine max	adenosine 5'-phosphosulfate	93.8	0.0247	3798	pH 8, 25 °C	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS) variant F245L, adenosine 5'-phosphosulfate
ATP sulfurylase isoform 1 (GmATPS)variant F245L	Glycine max	adenosine 5'-triphosphate	0.283	2.15	0.132	pH 8	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS) variant F245L, adenosine 5'-triphosphate
ATP sulfurylase isoform 1 (GmATPS)variant F245L	Glycine max	pyrophosphate	—	0.118	795	pH 8, 25 °C	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS) variant F245L, pyrophosphate
ATP sulfurylase isoform 1 (GmATPS)variant F245L	Glycine max	sulfate	—	0.306	0.093	pH 8	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS) variant F245L, sulfate
ATP sulfurylase isoform 1 (GmATPS)variant H252N	Glycine max	adenosine 5'-phosphosulfate	40.3	0.0272	1.48 × 10³	pH 8, 25 °C	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS) variant H252N, adenosine 5'-phosphosulfate
ATP sulfurylase isoform 1 (GmATPS)variant H252N	Glycine max	pyrophosphate	—	0.085	474	pH 8, 25 °C	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS) variant H252N, pyrophosphate
ATP sulfurylase isoform 1 (GmATPS)variant H255A	Glycine max	adenosine 5'-phosphosulfate	2.45	0.00441	556	pH 8, 25 °C	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS) variant H255A, adenosine 5'-phosphosulfate
ATP sulfurylase isoform 1 (GmATPS)variant H255A	Glycine max	pyrophosphate	—	0.0399	61.9	pH 8, 25 °C	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS) variant H255A, pyrophosphate
ATP sulfurylase isoform 1 (GmATPS)variant H255Q	Glycine max	adenosine 5'-phosphosulfate	63.5	0.0226	2.81 × 10³	pH 8, 25 °C	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS) variant H255Q, adenosine 5'-phosphosulfate
ATP sulfurylase isoform 1 (GmATPS)variant H255Q	Glycine max	pyrophosphate	—	0.114	557	pH 8, 25 °C	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS) variant H255Q, pyrophosphate
ATP sulfurylase isoform 1 (GmATPS)variant H333Q	Glycine max	adenosine 5'-phosphosulfate	27	0.0127	2.13 × 10³	pH 8, 25 °C	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS) variant H333Q, adenosine 5'-phosphosulfate
ATP sulfurylase isoform 1 (GmATPS)variant H333Q	Glycine max	adenosine 5'-triphosphate	0.162	0.575	0.281	pH 8	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS) variant H333Q, adenosine 5'-triphosphate
ATP sulfurylase isoform 1 (GmATPS)variant H333Q	Glycine max	pyrophosphate	—	0.022	1.13 × 10³	pH 8, 25 °C	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS) variant H333Q, pyrophosphate
ATP sulfurylase isoform 1 (GmATPS)variant H333Q	Glycine max	sulfate	—	0.311	0.52	pH 8	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS) variant H333Q, sulfate
ATP sulfurylase isoform 1 (GmATPS)variant L258A	Glycine max	adenosine 5'-phosphosulfate	109	0.0282	3.87 × 10³	pH 8, 25 °C	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS) variant L258A, adenosine 5'-phosphosulfate
ATP sulfurylase isoform 1 (GmATPS)variant L258A	Glycine max	pyrophosphate	—	0.373	292	pH 8, 25 °C	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS) variant L258A, pyrophosphate
ATP sulfurylase isoform 1 (GmATPS)variant L258V	Glycine max	adenosine 5'-phosphosulfate	129	0.0385	3.35 × 10³	pH 8, 25 °C	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS) variant L258V, adenosine 5'-phosphosulfate
ATP sulfurylase isoform 1 (GmATPS)variant L258V	Glycine max	pyrophosphate	—	0.208	629	pH 8, 25 °C	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS) variant L258V, pyrophosphate
ATP sulfurylase isoform 1 (GmATPS)variant N249A	Glycine max	adenosine 5'-phosphosulfate	34.6	0.0432	801	pH 8, 25 °C	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS) variant N249A, adenosine 5'-phosphosulfate
ATP sulfurylase isoform 1 (GmATPS)variant N249A	Glycine max	pyrophosphate	—	1.078	32.1	pH 8, 25 °C	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS) variant N249A, pyrophosphate
ATP sulfurylase isoform 1 (GmATPS)variant N249D	Glycine max	adenosine 5'-phosphosulfate	0.104	0.00651	16	pH 8, 25 °C	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS) variant N249D, adenosine 5'-phosphosulfate
ATP sulfurylase isoform 1 (GmATPS)variant N249D	Glycine max	pyrophosphate	—	0.0694	1.5	pH 8, 25 °C	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS) variant N249D, pyrophosphate
ATP sulfurylase isoform 1 (GmATPS)variant Q246A	Glycine max	adenosine 5'-phosphosulfate	64.3	0.0402	1.6 × 10³	pH 8, 25 °C	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS) variant Q246A, adenosine 5'-phosphosulfate
ATP sulfurylase isoform 1 (GmATPS)variant Q246A	Glycine max	adenosine 5'-triphosphate	0.288	0.346	0.833	pH 8	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS) variant Q246A, adenosine 5'-triphosphate
ATP sulfurylase isoform 1 (GmATPS)variant Q246A	Glycine max	pyrophosphate	—	0.117	373	pH 8, 25 °C	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS) variant Q246A, pyrophosphate
ATP sulfurylase isoform 1 (GmATPS)variant Q246A	Glycine max	sulfate	—	1.07	0.269	pH 8	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS) variant Q246A, sulfate
ATP sulfurylase isoform 1 (GmATPS)variant Q246E	Glycine max	adenosine 5'-phosphosulfate	10.3	0.312	33	pH 8, 25 °C	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS) variant Q246E, adenosine 5'-phosphosulfate
ATP sulfurylase isoform 1 (GmATPS)variant Q246E	Glycine max	pyrophosphate	—	0.0611	170	pH 8, 25 °C	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS) variant Q246E, pyrophosphate
ATP sulfurylase isoform 1 (GmATPS)variant Q246N	Glycine max	adenosine 5'-phosphosulfate	60.6	0.045	1.35 × 10³	pH 8, 25 °C	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS) variant Q246N, adenosine 5'-phosphosulfate
ATP sulfurylase isoform 1 (GmATPS)variant Q246N	Glycine max	adenosine 5'-triphosphate	0.134	0.88	0.155	pH 8	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS) variant Q246N, adenosine 5'-triphosphate
ATP sulfurylase isoform 1 (GmATPS)variant Q246N	Glycine max	pyrophosphate	—	0.0556	1.09 × 10³	pH 8, 25 °C	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS) variant Q246N, pyrophosphate
ATP sulfurylase isoform 1 (GmATPS)variant Q246N	Glycine max	sulfate	—	0.236	0.579	pH 8	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS) variant Q246N, sulfate
ATP sulfurylase isoform 1 (GmATPS)variant R248K	Glycine max	adenosine 5'-phosphosulfate	19.7	0.047	419	pH 8, 25 °C	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS) variant R248K, adenosine 5'-phosphosulfate
ATP sulfurylase isoform 1 (GmATPS)variant R248K	Glycine max	pyrophosphate	—	0.428	46	pH 8, 25 °C	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS) variant R248K, pyrophosphate
ATP sulfurylase isoform 1 (GmATPS)variant R349K	Glycine max	adenosine 5'-phosphosulfate	3.22	0.0346	93.1	pH 8, 25 °C	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS) variant R349K, adenosine 5'-phosphosulfate
ATP sulfurylase isoform 1 (GmATPS)variant R349K	Glycine max	pyrophosphate	—	0.59	5.46	pH 8, 25 °C	PMID 24584934	Open record: ATP sulfurylase isoform 1 (GmATPS) variant R349K, pyrophosphate
ATP sulfurylase, chloroplast form	Spinacia oleracea	adenosine-5'-phosphosulfateTable IV	218	3.7 × 10⁻⁴	5.9 × 10⁵ calc.	pH 8, 30 °C	PMID 8274013	Open record: ATP sulfurylase, chloroplast form, adenosine-5'-phosphosulfate
ATP sulfurylase, chloroplast form	Spinacia oleracea	adenosine-5'-phosphosulfatein the text	—	—	5.81 × 10⁵	pH 8, 30 °C	PMID 8274013	Open record: ATP sulfurylase, chloroplast form, adenosine-5'-phosphosulfate
ATP sulfurylase, chloroplast form	Spinacia oleracea	fluorophosphate	—	1.3	—	pH 8, 30 °C	PMID 8274013	Open record: ATP sulfurylase, chloroplast form, fluorophosphate
ATP sulfurylase, chloroplast form	Spinacia oleracea	inorganic pyrophosphateATP synthesis (reverse) reaction; 5 mM Mg2+; cosubstrate APS	—	0.01	—	pH 8, 30 °C	PMID 8274013	Open record: ATP sulfurylase, chloroplast form, inorganic pyrophosphate
ATP sulfurylase, chloroplast form	Spinacia oleracea	inorganic pyrophosphateMgCl2 5 mM in excess of PPi; cosubstrate β-F-ADP	—	0.25	—	pH 8, 30 °C	PMID 8274013	Open record: ATP sulfurylase, chloroplast form, inorganic pyrophosphate
ATP sulfurylase, chloroplast form	Spinacia oleracea	MgATPAPS synthesis (forward) reaction; cosubstrate SO4^2⁻	13	0.046	280 calc.	pH 8, 30 °C	PMID 8274013	Open record: ATP sulfurylase, chloroplast form, MgATP
ATP sulfurylase, chloroplast form	Spinacia oleracea	MgATPmolybdolysis reaction; with inorganic pyrophosphatase	187	0.045	4.2 × 10³ calc.	pH 8, 30 °C	PMID 8274013	Open record: ATP sulfurylase, chloroplast form, MgATP
ATP sulfurylase, chloroplast form	Spinacia oleracea	MgATPfluorophosphate activation reaction; cosubstrate FPO3^2⁻	—	0.5	—	pH 8, 30 °C	PMID 8274013	Open record: ATP sulfurylase, chloroplast form, MgATP
ATP sulfurylase, chloroplast form	Spinacia oleracea	molybdate	—	0.32	—	pH 8, 30 °C	PMID 8274013	Open record: ATP sulfurylase, chloroplast form, molybdate
ATP sulfurylase, chloroplast form	Spinacia oleracea	phosphate	—	20	—	pH 8, 30 °C	PMID 8274013	Open record: ATP sulfurylase, chloroplast form, phosphate
ATP sulfurylase, chloroplast form	Spinacia oleracea	sulfate	—	0.25	—	pH 8, 30 °C	PMID 8274013	Open record: ATP sulfurylase, chloroplast form, sulfate
ATP sulfurylase, cytosol form	Spinacia oleracea	adenosine-5'-phosphosulfate	202	5.3 × 10⁻⁴	3.8 × 10⁵ calc.	pH 8, 30 °C	PMID 8274013	Open record: ATP sulfurylase, cytosol form, adenosine-5'-phosphosulfate
ATP sulfurylase, cytosol form	Spinacia oleracea	inorganic pyrophosphate	—	0.017	—	pH 8, 30 °C	PMID 8274013	Open record: ATP sulfurylase, cytosol form, inorganic pyrophosphate
ATP sulfurylase, cytosol form	Spinacia oleracea	MgATPAPS synthesis (forward) reaction; cosubstrate SO4^2⁻	12	0.24	50 calc.	pH 8, 30 °C	PMID 8274013	Open record: ATP sulfurylase, cytosol form, MgATP
ATP sulfurylase, cytosol form	Spinacia oleracea	MgATPmolybdolysis reaction; cosubstrate MoO4^2⁻	98	0.15	650 calc.	pH 8, 30 °C	PMID 8274013	Open record: ATP sulfurylase, cytosol form, MgATP
ATP sulfurylase, cytosol form	Spinacia oleracea	molybdate	—	0.36	—	pH 8, 30 °C	PMID 8274013	Open record: ATP sulfurylase, cytosol form, molybdate
ATP sulfurylase, cytosol form	Spinacia oleracea	sulfate	—	0.87	—	pH 8, 30 °C	PMID 8274013	Open record: ATP sulfurylase, cytosol form, sulfate
PAPS synthase 1	Homo sapiens	adenosine 5'-triphosphate	0.017	0.08	0.21	pH 8, 37 °C	PMID 11931637	Open record: PAPS synthase 1, adenosine 5'-triphosphate
PAPS synthase 1	Homo sapiens	sulfate	0.019	0.43	0.045	pH 8, 37 °C	PMID 11931637	Open record: PAPS synthase 1, sulfate
PAPS synthase 2a	Homo sapiens	adenosine 5'-triphosphate	0.23	0.38	0.61	pH 8, 37 °C	PMID 11931637	Open record: PAPS synthase 2a, adenosine 5'-triphosphate
PAPS synthase 2a	Homo sapiens	sulfate	0.33	2.3	0.15	pH 8, 37 °C	PMID 11931637	Open record: PAPS synthase 2a, sulfate
PAPS synthase 2b	Homo sapiens	adenosine 5'-triphosphate	0.44	0.36	1.2	pH 8, 37 °C	PMID 11931637	Open record: PAPS synthase 2b, adenosine 5'-triphosphate
PAPS synthase 2b	Homo sapiens	sulfate	0.5	2.2	0.23	pH 8, 37 °C	PMID 11931637	Open record: PAPS synthase 2b, sulfate
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain	Homo sapiens	adenosine 5'-phosphosulfate	—	0.0048	—	pH 8, 30 °C	PMID 15065880	Open record: PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain, adenosine 5'-phosphosulfate
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain	Homo sapiens	chromate (CrO4 2-)APS kinase-coupled assay (ADP formation) with 50 µM Ap5A	—	0.05	—	pH 8, 30 °C	PMID 15065880	Open record: PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain, chromate (CrO4 2-)
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain	Homo sapiens	chromate (CrO4 2-)myokinase-coupled assay (AMP formation)	—	0.12	—	pH 8, 30 °C	PMID 15065880	Open record: PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain, chromate (CrO4 2-)
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain	Homo sapiens	hydrogen arsenate (HAsO4 2-)APS kinase-coupled assay (ADP formation) with 50 µM Ap5A	—	1.7	—	pH 8, 30 °C	PMID 15065880	Open record: PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain, hydrogen arsenate (HAsO4 2-)
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain	Homo sapiens	hydrogen arsenate (HAsO4 2-)myokinase-coupled assay (AMP formation)	—	18.5	—	pH 8, 30 °C	PMID 15065880	Open record: PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain, hydrogen arsenate (HAsO4 2-)
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain	Homo sapiens	hydrogen phosphate (HPO4 2-)	—	2.7	—	pH 8, 30 °C	PMID 15065880	Open record: PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain, hydrogen phosphate (HPO4 2-)
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain	Homo sapiens	inorganic pyrophosphate	—	0.0346	—	pH 8, 30 °C	PMID 15065880	Open record: PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain, inorganic pyrophosphate
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain	Homo sapiens	magnesium complex of adenosine 5'-triphosphate (MgATP)forward (APS synthesis) direction; 5 mM DTT in assay	—	0.15	—	pH 8, 30 °C	PMID 15065880	Open record: PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain, magnesium complex of adenosine 5'-triphosphate (MgATP)
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain	Homo sapiens	magnesium complex of adenosine 5'-triphosphate (MgATP)0.05 M Tris-HCl with 100 mM NaCl; 5 mM DTT in assay; Table 1	—	0.1	—	pH 8, 30 °C	PMID 15065880	Open record: PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain, magnesium complex of adenosine 5'-triphosphate (MgATP)
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain	Homo sapiens	magnesium complex of adenosine 5'-triphosphate (MgATP)forward (APS synthesis) direction; yeast PPiase; apparent Km	—	0.4	—	pH 8, 30 °C	PMID 15065880	Open record: PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain, magnesium complex of adenosine 5'-triphosphate (MgATP)
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain	Homo sapiens	magnesium complex of adenosine 5'-triphosphate (MgATP)high-salt assay (50 mM Tris-HCl + 100 mM NaCl); 5 mM DTT	—	0.49	—	pH 8, 30 °C	PMID 15065880	Open record: PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain, magnesium complex of adenosine 5'-triphosphate (MgATP)
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain	Homo sapiens	magnesium complex of adenosine 5'-triphosphate (MgATP)high-salt assay (50 mM Tris-HCl + 100 mM NaCl); 5 mM DTT	—	0.6	—	pH 8, 30 °C	PMID 15065880	Open record: PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain, magnesium complex of adenosine 5'-triphosphate (MgATP)
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain	Homo sapiens	magnesium complex of adenosine 5'-triphosphate (MgATP)high-salt assay (50 mM Tris-HCl + 100 mM NaCl); MgATP varied	—	0.4	—	pH 8, 30 °C	PMID 15065880	Open record: PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain, magnesium complex of adenosine 5'-triphosphate (MgATP)
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain	Homo sapiens	magnesium complex of adenosine 5'-triphosphate (MgATP)high-salt assay (50 mM Tris-HCl + 100 mM NaCl); MgATP varied	—	0.06	—	pH 8, 30 °C	PMID 15065880	Open record: PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain, magnesium complex of adenosine 5'-triphosphate (MgATP)
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain	Homo sapiens	magnesium complex of adenosine 5'-triphosphate (MgATP)high-salt assay (50 mM Tris-HCl + 100 mM NaCl); 5 mM DTT	—	0.07	—	pH 8, 30 °C	PMID 15065880	Open record: PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain, magnesium complex of adenosine 5'-triphosphate (MgATP)
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain	Homo sapiens	magnesium complex of adenosine 5'-triphosphate (MgATP)high-salt assay (50 mM Tris-HCl + 100 mM NaCl); 5 mM DTT	—	0.16	—	pH 8, 30 °C	PMID 15065880	Open record: PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain, magnesium complex of adenosine 5'-triphosphate (MgATP)
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain	Homo sapiens	magnesium complex of adenosine 5'-triphosphate (MgATP)high-salt assay (50 mM Tris-HCl + 100 mM NaCl); 5 mM DTT	—	5	—	pH 8, 30 °C	PMID 15065880	Open record: PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain, magnesium complex of adenosine 5'-triphosphate (MgATP)
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain	Homo sapiens	magnesium complex of adenosine 5'-triphosphate (MgATP)high-salt assay (50 mM Tris-HCl + 100 mM NaCl); 5 mM DTT	—	1.8	—	pH 8, 30 °C	PMID 15065880	Open record: PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain, magnesium complex of adenosine 5'-triphosphate (MgATP)
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain	Homo sapiens	magnesium complex of adenosine 5'-triphosphate (MgATP)inorganic substrate phosphate fixed at 5 mM; MgATP varied	—	1.5	—	pH 8, 30 °C	PMID 15065880	Open record: PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain, magnesium complex of adenosine 5'-triphosphate (MgATP)
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain	Homo sapiens	molybdate	—	1.3	—	pH 8, 30 °C	PMID 15065880	Open record: PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain, molybdate
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain	Homo sapiens	molybdate (MoO4 2-)	—	1.5	—	pH 8, 30 °C	PMID 15065880	Open record: PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain, molybdate (MoO4 2-)
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain	Homo sapiens	selenate (SeO4 2-)APS kinase-coupled assay (ADP formation) with 50 µM Ap5A	—	0.35	—	pH 8, 30 °C	PMID 15065880	Open record: PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain, selenate (SeO4 2-)
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain	Homo sapiens	selenate (SeO4 2-)myokinase-coupled assay (AMP formation)	—	0.52	—	pH 8, 30 °C	PMID 15065880	Open record: PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain, selenate (SeO4 2-)
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain	Homo sapiens	sulfate0.05 M Tris-HCl with 100 mM NaCl; 5 mM DTT in assay	—	0.16	—	pH 8, 30 °C	PMID 15065880	Open record: PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain, sulfate
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain	Homo sapiens	sulfateoptimum conditions (high salt, 100 mM NaCl, with 5 mM DTT)	—	0.19	—	pH 8, 30 °C	PMID 15065880	Open record: PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain, sulfate
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain	Homo sapiens	tungstate (WO4 2-)	—	4.5	—	pH 8, 30 °C	PMID 15065880	Open record: PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain, tungstate (WO4 2-)