CatLogenzyme kinetics catalog

Values CatLog read from this paper

Human 3'-phosphoadenosine 5'-phosphosulfate synthetase (isoform 1, brain): kinetic properties of the adenosine triphosphate sulfurylase and adenosine 5'-phosphosulfate kinase domains

PMID 15065880doi:10.1021/bi049827mOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 28 records (28 values) from this paper: PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domain, PAPS synthetase isoform 1 (PAPSS1, brain), APS kinase domain from Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.7.1.25, EC 2.7.7.4

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
PAPS synthetase isoform 1 (PAPSS1, brain), APS kinase domainHomo sapiensadenosine 5'-phosphosulfate—0.0042—pH 8, 30 °COpen
PAPS synthetase isoform 1 (PAPSS1, brain), APS kinase domainHomo sapiensmagnesium complex of adenosine 5'-triphosphate (MgATP)—0.14—pH 8, 30 °COpen
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domainHomo sapiensadenosine 5'-phosphosulfate—0.0048—pH 8, 30 °COpen
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domainHomo sapienschromate (CrO4 2-)APS kinase-coupled assay (ADP formation) with 50 µM Ap5A—0.05—pH 8, 30 °COpen
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domainHomo sapienschromate (CrO4 2-)myokinase-coupled assay (AMP formation)—0.12—pH 8, 30 °COpen
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domainHomo sapienshydrogen arsenate (HAsO4 2-)APS kinase-coupled assay (ADP formation) with 50 µM Ap5A—1.7—pH 8, 30 °COpen
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domainHomo sapienshydrogen arsenate (HAsO4 2-)myokinase-coupled assay (AMP formation)—18.5—pH 8, 30 °COpen
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domainHomo sapienshydrogen phosphate (HPO4 2-)—2.7—pH 8, 30 °COpen
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domainHomo sapiensinorganic pyrophosphate—0.0346—pH 8, 30 °COpen
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domainHomo sapiensmagnesium complex of adenosine 5'-triphosphate (MgATP)forward (APS synthesis) direction; 5 mM DTT in assay—0.15—pH 8, 30 °COpen
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domainHomo sapiensmagnesium complex of adenosine 5'-triphosphate (MgATP)0.05 M Tris-HCl with 100 mM NaCl; 5 mM DTT in assay; Table 1—0.1—pH 8, 30 °COpen
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domainHomo sapiensmagnesium complex of adenosine 5'-triphosphate (MgATP)forward (APS synthesis) direction; yeast PPiase; apparent Km—0.4—pH 8, 30 °COpen
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domainHomo sapiensmagnesium complex of adenosine 5'-triphosphate (MgATP)high-salt assay (50 mM Tris-HCl + 100 mM NaCl); 5 mM DTT—0.49—pH 8, 30 °COpen
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domainHomo sapiensmagnesium complex of adenosine 5'-triphosphate (MgATP)high-salt assay (50 mM Tris-HCl + 100 mM NaCl); 5 mM DTT—0.6—pH 8, 30 °COpen
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domainHomo sapiensmagnesium complex of adenosine 5'-triphosphate (MgATP)high-salt assay (50 mM Tris-HCl + 100 mM NaCl); MgATP varied—0.4—pH 8, 30 °COpen
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domainHomo sapiensmagnesium complex of adenosine 5'-triphosphate (MgATP)high-salt assay (50 mM Tris-HCl + 100 mM NaCl); MgATP varied—0.06—pH 8, 30 °COpen
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domainHomo sapiensmagnesium complex of adenosine 5'-triphosphate (MgATP)high-salt assay (50 mM Tris-HCl + 100 mM NaCl); 5 mM DTT—0.07—pH 8, 30 °COpen
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domainHomo sapiensmagnesium complex of adenosine 5'-triphosphate (MgATP)high-salt assay (50 mM Tris-HCl + 100 mM NaCl); 5 mM DTT—0.16—pH 8, 30 °COpen
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domainHomo sapiensmagnesium complex of adenosine 5'-triphosphate (MgATP)high-salt assay (50 mM Tris-HCl + 100 mM NaCl); 5 mM DTT—5—pH 8, 30 °COpen
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domainHomo sapiensmagnesium complex of adenosine 5'-triphosphate (MgATP)high-salt assay (50 mM Tris-HCl + 100 mM NaCl); 5 mM DTT—1.8—pH 8, 30 °COpen
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domainHomo sapiensmagnesium complex of adenosine 5'-triphosphate (MgATP)inorganic substrate phosphate fixed at 5 mM; MgATP varied—1.5—pH 8, 30 °COpen
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domainHomo sapiensmolybdate—1.3—pH 8, 30 °COpen
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domainHomo sapiensmolybdate (MoO4 2-)—1.5—pH 8, 30 °COpen
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domainHomo sapiensselenate (SeO4 2-)APS kinase-coupled assay (ADP formation) with 50 µM Ap5A—0.35—pH 8, 30 °COpen
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domainHomo sapiensselenate (SeO4 2-)myokinase-coupled assay (AMP formation)—0.52—pH 8, 30 °COpen
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domainHomo sapienssulfate0.05 M Tris-HCl with 100 mM NaCl; 5 mM DTT in assay—0.16—pH 8, 30 °COpen
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domainHomo sapienssulfateoptimum conditions (high salt, 100 mM NaCl, with 5 mM DTT)—0.19—pH 8, 30 °COpen
PAPS synthetase isoform 1 (PAPSS1, brain), ATP sulfurylase domainHomo sapienstungstate (WO4 2-)—4.5—pH 8, 30 °COpen