Values CatLog read from this paper
Paenibacillus sp. TS12 glucosylceramidase: kinetic studies of a novel sub-family of family 3 glycosidases and identification of the catalytic residues
PMID 14561218doi:10.1042/bj20031028Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 6 records (18 values) from this paper: glucosylceramidase (GCase) from Paenibacillus sp. TS12.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| glucosylceramidase (GCase) | Paenibacillus sp. TS12 | 2,4-dinitrophenyl beta-D-glucopyranoside | 470 | 0.16 | 2.9 × 10³ | pH 6.5, 37 °C | Open record: glucosylceramidase (GCase), 2,4-dinitrophenyl beta-D-glucopyranoside |
| glucosylceramidase (GCase) | Paenibacillus sp. TS12 | p-nitrophenyl beta-D-glucopyranoside | 560 | 0.27 | 2.1 × 10³ | pH 6.5, 37 °C | Open record: glucosylceramidase (GCase), p-nitrophenyl beta-D-glucopyranoside |
| glucosylceramidase (GCase)variant D223G | Paenibacillus sp. TS12 | 2,4-dinitrophenyl beta-D-glucopyranoside | 0.012 | 0.15 | 0.079 | pH 6.5, 37 °C | Open record: glucosylceramidase (GCase) variant D223G, 2,4-dinitrophenyl beta-D-glucopyranoside |
| glucosylceramidase (GCase)variant D223G | Paenibacillus sp. TS12 | p-nitrophenyl beta-D-glucopyranoside | 0.012 | 0.33 | 0.036 | pH 6.5, 37 °C | Open record: glucosylceramidase (GCase) variant D223G, p-nitrophenyl beta-D-glucopyranoside |
| glucosylceramidase (GCase)variant E411A | Paenibacillus sp. TS12 | 2,4-dinitrophenyl beta-D-glucopyranoside | 0.067 | 3 × 10⁻⁴ | 200 | pH 6.5, 37 °C | Open record: glucosylceramidase (GCase) variant E411A, 2,4-dinitrophenyl beta-D-glucopyranoside |
| glucosylceramidase (GCase)variant E411A | Paenibacillus sp. TS12 | p-nitrophenyl beta-D-glucopyranoside | 0.099 | 0.018 | 5.5 | pH 6.5, 37 °C | Open record: glucosylceramidase (GCase) variant E411A, p-nitrophenyl beta-D-glucopyranoside |