EC 3.2.1.45: glucosylceramidase
HydrolasesOpen in Browse
CatLog read 24 records for EC 3.2.1.45 from 3 papers, for 3 organisms. Names in these papers: Os3BGlu6 (GH1 glucocerebrosidase), TxGH116 β-glucosidase, glucosylceramidase (GCase).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 15 records for EC 3.2.1.45 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.2.1.45 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Bacterial β-Glucosidase Reveals the Structural and Functional Basis of Genetic Defects in Human Glucocerebrosidase 2 (GBA2) | Thermoanaerobacterium xylanolyticum | 9 |
| Sphingadienine-1-phosphate levels are regulated by a novel glycoside hydrolase family 1 glucocerebrosidase widely distributed in seed plants | Oryza sativa | 9 |
| Paenibacillus sp. TS12 glucosylceramidase: kinetic studies of a novel sub-family of family 3 glycosidases and identification of the catalytic residues | Paenibacillus sp. TS12 | 6 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| glucosylceramidase (GCase) | Paenibacillus sp. TS12 | 2,4-dinitrophenyl beta-D-glucopyranoside | 470 | 0.16 | 2.9 × 10³ | pH 6.5, 37 °C | PMID 14561218 | Open record: glucosylceramidase (GCase), 2,4-dinitrophenyl beta-D-glucopyranoside |
| glucosylceramidase (GCase) | Paenibacillus sp. TS12 | p-nitrophenyl beta-D-glucopyranoside | 560 | 0.27 | 2.1 × 10³ | pH 6.5, 37 °C | PMID 14561218 | Open record: glucosylceramidase (GCase), p-nitrophenyl beta-D-glucopyranoside |
| glucosylceramidase (GCase)variant D223G | Paenibacillus sp. TS12 | 2,4-dinitrophenyl beta-D-glucopyranoside | 0.012 | 0.15 | 0.079 | pH 6.5, 37 °C | PMID 14561218 | Open record: glucosylceramidase (GCase) variant D223G, 2,4-dinitrophenyl beta-D-glucopyranoside |
| glucosylceramidase (GCase)variant D223G | Paenibacillus sp. TS12 | p-nitrophenyl beta-D-glucopyranoside | 0.012 | 0.33 | 0.036 | pH 6.5, 37 °C | PMID 14561218 | Open record: glucosylceramidase (GCase) variant D223G, p-nitrophenyl beta-D-glucopyranoside |
| glucosylceramidase (GCase)variant E411A | Paenibacillus sp. TS12 | 2,4-dinitrophenyl beta-D-glucopyranoside | 0.067 | 3 × 10⁻⁴ | 200 | pH 6.5, 37 °C | PMID 14561218 | Open record: glucosylceramidase (GCase) variant E411A, 2,4-dinitrophenyl beta-D-glucopyranoside |
| glucosylceramidase (GCase)variant E411A | Paenibacillus sp. TS12 | p-nitrophenyl beta-D-glucopyranoside | 0.099 | 0.018 | 5.5 | pH 6.5, 37 °C | PMID 14561218 | Open record: glucosylceramidase (GCase) variant E411A, p-nitrophenyl beta-D-glucopyranoside |
| Os3BGlu6 (GH1 glucocerebrosidase) | Oryza sativa | (4E,8E)-N-D-2′-hydroxy-(E)-3′-hexadecenoyl-1-O-β-D-glucopyranosyl-9-methyl-4,8-sphingadienine | 151 | 0.0411 | 3.7 × 10³ | pH 5.5, 37 °C | PMID 34563538 | Open record: Os3BGlu6 (GH1 glucocerebrosidase), (4E,8E)-N-D-2′-hydroxy-(E)-3′-hexadecenoyl-1-O-β-D-glucopyranosyl-9-methyl-4,8-sphingadienine |
| Os3BGlu6 (GH1 glucocerebrosidase) | Oryza sativa | (4E,8E)-N-D-2′-hydroxypalmitoyl-1-O-β-D-glucopyranosyl-9-methyl-4,8-sphingadienine15 min; enzyme preincubated in Buffer L 30 min at 37 °C | 144.6 | 0.0381 | 3.79 × 10³ | pH 5.5, 37 °C | PMID 34563538 | Open record: Os3BGlu6 (GH1 glucocerebrosidase), (4E,8E)-N-D-2′-hydroxypalmitoyl-1-O-β-D-glucopyranosyl-9-methyl-4,8-sphingadienine |
| Os3BGlu6 (GH1 glucocerebrosidase) | Oryza sativa | (4E,8E)-N-D-2′-hydroxypalmitoyl-1-O-β-D-glucopyranosyl-9-methyl-4,8-sphingadieninenot glycosylated | 134.4 | 0.215 | 625 calc. | pH 5.5, 37 °C | PMID 34563538 | Open record: Os3BGlu6 (GH1 glucocerebrosidase), (4E,8E)-N-D-2′-hydroxypalmitoyl-1-O-β-D-glucopyranosyl-9-methyl-4,8-sphingadienine |
| Os3BGlu6 (GH1 glucocerebrosidase) | Oryza sativa | d18:1(4E)-C12:0-GluCer | 121.1 | 0.0461 | 2.64 × 10³ | pH 5.5, 37 °C | PMID 34563538 | Open record: Os3BGlu6 (GH1 glucocerebrosidase), d18:1(4E)-C12:0-GluCer |
| Os3BGlu6 (GH1 glucocerebrosidase) | Oryza sativa | d18:1(4E)-C8:0-GluCer | 156.1 | 0.0475 | 3.29 × 10³ | pH 5.5, 37 °C | PMID 34563538 | Open record: Os3BGlu6 (GH1 glucocerebrosidase), d18:1(4E)-C8:0-GluCer |
| Os3BGlu6 (GH1 glucocerebrosidase) | Oryza sativa | d18:2(4E,8E)-C16h:0-GluCer | 145.8 | 0.05 | 2.92 × 10³ | pH 5.5, 37 °C | PMID 34563538 | Open record: Os3BGlu6 (GH1 glucocerebrosidase), d18:2(4E,8E)-C16h:0-GluCer |
| Os3BGlu6 (GH1 glucocerebrosidase) | Oryza sativa | d18:2(4E,8Z)-C16h:0-GluCer | 115.8 | 0.0198 | 5.85 × 10³ | pH 5.5, 37 °C | PMID 34563538 | Open record: Os3BGlu6 (GH1 glucocerebrosidase), d18:2(4E,8Z)-C16h:0-GluCer |
| Os3BGlu6 (GH1 glucocerebrosidase) | Oryza sativa | N-[6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl]-D-glucosyl-β1-1′-sphingosine | 112.1 | 0.0571 | 1.97 × 10³ | pH 5.5, 37 °C | PMID 34563538 | Open record: Os3BGlu6 (GH1 glucocerebrosidase), N-[6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl]-D-glucosyl-β1-1′-sphingosine |
| Os3BGlu6 (GH1 glucocerebrosidase) | Oryza sativa | p-nitrophenyl β-D-glucopyranoside | 285.1 | 3.825 | 75 | pH 5.5, 37 °C | PMID 34563538 | Open record: Os3BGlu6 (GH1 glucocerebrosidase), p-nitrophenyl β-D-glucopyranoside |
| TxGH116 β-glucosidase | Thermoanaerobacterium xylanolyticum | 4-nitrophenyl beta-D-galactopyranoside | 255 | 16.3 | 15.6 | 60 °C | PMID 27115290 | Open record: TxGH116 β-glucosidase, 4-nitrophenyl beta-D-galactopyranoside |
| TxGH116 β-glucosidase | Thermoanaerobacterium xylanolyticum | 4-nitrophenyl beta-D-glucopyranosideTxGH116Δ1−18 (lacking the first 18 residues) | 49 | 0.18 | 272 | 60 °C | PMID 27115290 | Open record: TxGH116 β-glucosidase, 4-nitrophenyl beta-D-glucopyranoside |
| TxGH116 β-glucosidase | Thermoanaerobacterium xylanolyticum | 4-nitrophenyl beta-D-glucopyranosideS-tag and enterokinase site (not removed) | 37.6 | 0.21 | 179 | 60 °C | PMID 27115290 | Open record: TxGH116 β-glucosidase, 4-nitrophenyl beta-D-glucopyranoside |
| TxGH116 β-glucosidase | Thermoanaerobacterium xylanolyticum | cellobiose | 44.4 | 0.25 | 178 | 60 °C | PMID 27115290 | Open record: TxGH116 β-glucosidase, cellobiose |
| TxGH116 β-glucosidase | Thermoanaerobacterium xylanolyticum | laminaribiose | 41.4 | 0.27 | 153 | 60 °C | PMID 27115290 | Open record: TxGH116 β-glucosidase, laminaribiose |
| TxGH116 β-glucosidasevariant D508H | Thermoanaerobacterium xylanolyticum | 4-nitrophenyl beta-D-glucopyranoside | 3.47 | 110 | 0.031 | 60 °C | PMID 27115290 | Open record: TxGH116 β-glucosidase variant D508H, 4-nitrophenyl beta-D-glucopyranoside |
| TxGH116 β-glucosidasevariant D508N | Thermoanaerobacterium xylanolyticum | 4-nitrophenyl beta-D-glucopyranoside | 30.2 | 41 | 0.74 | 60 °C | PMID 27115290 | Open record: TxGH116 β-glucosidase variant D508N, 4-nitrophenyl beta-D-glucopyranoside |
| TxGH116 β-glucosidasevariant R544W | Thermoanaerobacterium xylanolyticum | 4-nitrophenyl beta-D-glucopyranoside | 48.2 | 0.26 | 186 | 60 °C | PMID 27115290 | Open record: TxGH116 β-glucosidase variant R544W, 4-nitrophenyl beta-D-glucopyranoside |
| TxGH116 β-glucosidasevariant R786H | Thermoanaerobacterium xylanolyticum | 4-nitrophenyl beta-D-glucopyranoside | 193 | 15.7 | 12.3 | 60 °C | PMID 27115290 | Open record: TxGH116 β-glucosidase variant R786H, 4-nitrophenyl beta-D-glucopyranoside |