Values CatLog read from this paper
Sphingadienine-1-phosphate levels are regulated by a novel glycoside hydrolase family 1 glucocerebrosidase widely distributed in seed plants
PMID 34563538doi:10.1016/j.jbc.2021.101236Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 9 records (26 values) from this paper: Os3BGlu6 (GH1 glucocerebrosidase) from Oryza sativa.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| Os3BGlu6 (GH1 glucocerebrosidase) | Oryza sativa | (4E,8E)-N-D-2′-hydroxy-(E)-3′-hexadecenoyl-1-O-β-D-glucopyranosyl-9-methyl-4,8-sphingadienine | 151 | 0.0411 | 3.7 × 10³ | pH 5.5, 37 °C | Open record: Os3BGlu6 (GH1 glucocerebrosidase), (4E,8E)-N-D-2′-hydroxy-(E)-3′-hexadecenoyl-1-O-β-D-glucopyranosyl-9-methyl-4,8-sphingadienine |
| Os3BGlu6 (GH1 glucocerebrosidase) | Oryza sativa | (4E,8E)-N-D-2′-hydroxypalmitoyl-1-O-β-D-glucopyranosyl-9-methyl-4,8-sphingadienine15 min; enzyme preincubated in Buffer L 30 min at 37 °C | 144.6 | 0.0381 | 3.79 × 10³ | pH 5.5, 37 °C | Open record: Os3BGlu6 (GH1 glucocerebrosidase), (4E,8E)-N-D-2′-hydroxypalmitoyl-1-O-β-D-glucopyranosyl-9-methyl-4,8-sphingadienine |
| Os3BGlu6 (GH1 glucocerebrosidase) | Oryza sativa | (4E,8E)-N-D-2′-hydroxypalmitoyl-1-O-β-D-glucopyranosyl-9-methyl-4,8-sphingadieninenot glycosylated | 134.4 | 0.215 | 625 calc. | pH 5.5, 37 °C | Open record: Os3BGlu6 (GH1 glucocerebrosidase), (4E,8E)-N-D-2′-hydroxypalmitoyl-1-O-β-D-glucopyranosyl-9-methyl-4,8-sphingadienine |
| Os3BGlu6 (GH1 glucocerebrosidase) | Oryza sativa | d18:1(4E)-C12:0-GluCer | 121.1 | 0.0461 | 2.64 × 10³ | pH 5.5, 37 °C | Open record: Os3BGlu6 (GH1 glucocerebrosidase), d18:1(4E)-C12:0-GluCer |
| Os3BGlu6 (GH1 glucocerebrosidase) | Oryza sativa | d18:1(4E)-C8:0-GluCer | 156.1 | 0.0475 | 3.29 × 10³ | pH 5.5, 37 °C | Open record: Os3BGlu6 (GH1 glucocerebrosidase), d18:1(4E)-C8:0-GluCer |
| Os3BGlu6 (GH1 glucocerebrosidase) | Oryza sativa | d18:2(4E,8E)-C16h:0-GluCer | 145.8 | 0.05 | 2.92 × 10³ | pH 5.5, 37 °C | Open record: Os3BGlu6 (GH1 glucocerebrosidase), d18:2(4E,8E)-C16h:0-GluCer |
| Os3BGlu6 (GH1 glucocerebrosidase) | Oryza sativa | d18:2(4E,8Z)-C16h:0-GluCer | 115.8 | 0.0198 | 5.85 × 10³ | pH 5.5, 37 °C | Open record: Os3BGlu6 (GH1 glucocerebrosidase), d18:2(4E,8Z)-C16h:0-GluCer |
| Os3BGlu6 (GH1 glucocerebrosidase) | Oryza sativa | N-[6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl]-D-glucosyl-β1-1′-sphingosine | 112.1 | 0.0571 | 1.97 × 10³ | pH 5.5, 37 °C | Open record: Os3BGlu6 (GH1 glucocerebrosidase), N-[6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl]-D-glucosyl-β1-1′-sphingosine |
| Os3BGlu6 (GH1 glucocerebrosidase) | Oryza sativa | p-nitrophenyl β-D-glucopyranoside | 285.1 | 3.825 | 75 | pH 5.5, 37 °C | Open record: Os3BGlu6 (GH1 glucocerebrosidase), p-nitrophenyl β-D-glucopyranoside |