CatLogenzyme kinetics catalog

Values CatLog read from this paper

Site-directed mutagenesis of Escherichia coli acetylglutamate kinase and aspartokinase III probes the catalytic and substrate-binding mechanisms of these amino acid kinase family enzymes and allows…

PMID 14623187doi:10.1016/j.jmb.2003.09.038Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 22 records (22 values) from this paper: N-acetyl-L-glutamate kinase (NAGK), aspartokinase III (AKIII) from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.7.2.4, EC 2.7.2.8

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
aspartokinase III (AKIII)Escherichia coliATP—0.2—pH 7.2, 25 °COpen
aspartokinase III (AKIII)Escherichia coliL-aspartate—0.69—pH 7.2, 25 °COpen
aspartokinase III (AKIII)variant D202EEscherichia coliATP—0.52—pH 7.2, 25 °COpen
aspartokinase III (AKIII)variant D202EEscherichia coliL-aspartate—29—pH 7.2, 25 °COpen
aspartokinase III (AKIII)variant E119DEscherichia coliATP—2.3—pH 7.2, 25 °COpen
aspartokinase III (AKIII)variant E119DEscherichia coliL-aspartate—2152—pH 7.2, 25 °COpen
aspartokinase III (AKIII)variant K8REscherichia coliATP—0.36—pH 7.2, 25 °COpen
aspartokinase III (AKIII)variant K8REscherichia coliL-aspartate—26—pH 7.2, 25 °COpen
aspartokinase III (AKIII)variant R198KEscherichia coliATP—3.9—pH 7.2, 25 °COpen
aspartokinase III (AKIII)variant R198KEscherichia coliL-aspartate—114—pH 7.2, 25 °COpen
N-acetyl-L-glutamate kinase (NAGK)Escherichia coliMgATPfixed 0.1 M N-acetyl-L-glutamate—0.29—pH 7, 25 °COpen
N-acetyl-L-glutamate kinase (NAGK)Escherichia coliMgATPfixed 10 mM N-acetyl-L-glutamate—0.31—pH 7, 25 °COpen
N-acetyl-L-glutamate kinase (NAGK)Escherichia coliN-acetyl-L-glutamate—0.2—pH 7, 25 °COpen
N-acetyl-L-glutamate kinase (NAGK)variant D162EEscherichia coliMgATP—0.46—pH 7, 25 °COpen
N-acetyl-L-glutamate kinase (NAGK)variant D162EEscherichia coliN-acetyl-L-glutamate—0.37—pH 7, 25 °COpen
N-acetyl-L-glutamate kinase (NAGK)variant K8REscherichia coliMgATP—3.3—pH 7, 25 °COpen
N-acetyl-L-glutamate kinase (NAGK)variant K8REscherichia coliN-acetyl-L-glutamate—2.3—pH 7, 25 °COpen
N-acetyl-L-glutamate kinase (NAGK)variant N158QEscherichia coliMgATP—7.9—pH 7, 25 °COpen
N-acetyl-L-glutamate kinase (NAGK)variant N158QEscherichia coliN-acetyl-L-glutamate—600—pH 7, 25 °COpen
N-acetyl-L-glutamate kinase (NAGK)variant R66KEscherichia coliMgATP—5.2—pH 7, 25 °COpen
N-acetyl-L-glutamate kinase (NAGK)variant R66KEscherichia coliN-acetyl-L-glutamate—898—pH 7, 25 °COpen
N-acetyl-L-glutamate kinase (NAGK)variant R66KEscherichia coliN-acetyl-L-glutamate—4 × 10³—pH 7, 25 °COpen