Values CatLog read from this paper
Molecular mechanism of the enterococcal aminoglycoside 6'-N-acetyltransferase': role of GNAT-conserved residues in the chemistry of antibiotic inactivation
PMID 14717599doi:10.1021/bi035667nOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 39 records (117 values) from this paper: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii from Enterococcus faecium.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant E72A | Enterococcus faecium | acetyl-CoA | 0.99 | 0.154 | 6.4 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant E72A, acetyl-CoA |
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant E72A | Enterococcus faecium | amikacin | 0.05 | 0.38 | 0.13 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant E72A, amikacin |
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant E72A | Enterococcus faecium | kanamycin A | 0.55 | 3.49 | 0.16 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant E72A, kanamycin A |
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant E72A | Enterococcus faecium | neamine | 0.44 | 0.482 | 0.91 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant E72A, neamine |
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant E72A | Enterococcus faecium | neomycin | 1.1 | 0.029 | 38 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant E72A, neomycin |
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant E72A | Enterococcus faecium | ribostamycin | 0.35 | 0.301 | 1.2 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant E72A, ribostamycin |
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant E72A | Enterococcus faecium | tobramycin | 1.1 | 1.15 | 0.96 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant E72A, tobramycin |
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant H74A | Enterococcus faecium | acetyl-CoA | 0.18 | 0.0073 | 25 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant H74A, acetyl-CoA |
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant H74A | Enterococcus faecium | amikacin | 0.04 | 0.0582 | 0.71 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant H74A, amikacin |
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant H74A | Enterococcus faecium | kanamycin A | 0.05 | 0.0144 | 3.3 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant H74A, kanamycin A |
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant H74A | Enterococcus faecium | neamine | 0.15 | 0.0432 | 3.5 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant H74A, neamine |
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant H74A | Enterococcus faecium | neomycin | 0.19 | 0.0182 | 10 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant H74A, neomycin |
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant H74A | Enterococcus faecium | ribostamycin | 0.23 | 0.0381 | 6 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant H74A, ribostamycin |
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant H74A | Enterococcus faecium | tobramycin | 0.3 | 0.0369 | 8.2 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant H74A, tobramycin |
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant L76A | Enterococcus faecium | acetyl-CoA | 0.23 | 0.0033 | 69 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant L76A, acetyl-CoA |
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant L76A | Enterococcus faecium | amikacin | 0.03 | 0.0114 | 2.5 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant L76A, amikacin |
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant L76A | Enterococcus faecium | kanamycin A | 0.27 | 0.0257 | 11 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant L76A, kanamycin A |
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant L76A | Enterococcus faecium | neamine | 0.38 | 0.0224 | 17 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant L76A, neamine |
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant L76A | Enterococcus faecium | neomycin | 0.16 | 0.019 | 8.4 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant L76A, neomycin |
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant L76A | Enterococcus faecium | ribostamycin | 0.15 | 0.0214 | 7 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant L76A, ribostamycin |
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant L76A | Enterococcus faecium | tobramycin | 0.89 | 0.062 | 14 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant L76A, tobramycin |
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant L76P | Enterococcus faecium | acetyl-CoA | 0.06 | 0.0489 | 1.2 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant L76P, acetyl-CoA |
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant L76P | Enterococcus faecium | neamine | 0.002 | 0.0682 | 0.031 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant L76P, neamine |
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant L76P | Enterococcus faecium | neomycin | 0.04 | 0.0573 | 0.65 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant L76P, neomycin |
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant L76P | Enterococcus faecium | ribostamycin | 0.01 | 0.107 | 0.077 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant L76P, ribostamycin |
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant L76P | Enterococcus faecium | tobramycin | 0.01 | 0.146 | 0.038 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant L76P, tobramycin |
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant Y147A | Enterococcus faecium | acetyl-CoA | 0.01 | 0.0047 | 1.6 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant Y147A, acetyl-CoA |
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant Y147A | Enterococcus faecium | kanamycin A | 0.01 | 0.11 | 0.056 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant Y147A, kanamycin A |
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant Y147A | Enterococcus faecium | neamine | 0.004 | 0.169 | 0.022 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant Y147A, neamine |
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant Y147A | Enterococcus faecium | neomycin | 0.001 | 0.0386 | 0.036 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant Y147A, neomycin |
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant Y147A | Enterococcus faecium | ribostamycin | 0.02 | 0.109 | 0.15 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant Y147A, ribostamycin |
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant Y147A | Enterococcus faecium | tobramycin | 0.004 | 0.147 | 0.026 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant Y147A, tobramycin |
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant Y147F | Enterococcus faecium | acetyl-CoA | 0.07 | 0.0041 | 18 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant Y147F, acetyl-CoA |
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant Y147F | Enterococcus faecium | amikacin | 0.003 | 2.19 | 0.0012 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant Y147F, amikacin |
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant Y147F | Enterococcus faecium | kanamycin A | 0.03 | 0.34 | 0.1 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant Y147F, kanamycin A |
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant Y147F | Enterococcus faecium | neamine | 0.05 | 0.15 | 0.32 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant Y147F, neamine |
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant Y147F | Enterococcus faecium | neomycin | 0.07 | 0.0553 | 1.3 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant Y147F, neomycin |
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant Y147F | Enterococcus faecium | ribostamycin | 0.12 | 0.306 | 0.4 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant Y147F, ribostamycin |
| aminoglycoside 6'-N-acetyltransferase AAC(6')-Iivariant Y147F | Enterococcus faecium | tobramycin | 0.1 | 0.136 | 0.71 | pH 6, 37 °C | Open record: aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii variant Y147F, tobramycin |