Values CatLog read from this paper
Mutational and structural analysis of cobalt-containing nitrile hydratase on substrate and metal binding
PMID 14717710doi:10.1046/j.1432-1033.2003.03943.xOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 14 records (42 values) from this paper: nitrile hydratase (cobalt-containing, Co-type NHase; α2β2) from Pseudonocardia thermophila.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| nitrile hydratase (cobalt-containing, Co-type NHase; α2β2) | Pseudonocardia thermophila | 3-cyanopyridine | 131 | 0.12 | 1.09 × 10³ | pH 7.6, 25 °C | Open record: nitrile hydratase (cobalt-containing, Co-type NHase; α2β2), 3-cyanopyridine |
| nitrile hydratase (cobalt-containing, Co-type NHase; α2β2) | Pseudonocardia thermophila | 4-cyanopyridine | 90 | 0.079 | 1.14 × 10³ | pH 7.6, 25 °C | Open record: nitrile hydratase (cobalt-containing, Co-type NHase; α2β2), 4-cyanopyridine |
| nitrile hydratase (cobalt-containing, Co-type NHase; α2β2) | Pseudonocardia thermophila | Acrylonitrile | 1.91 × 10³ | 3.6 | 537 | pH 7.6, 25 °C | Open record: nitrile hydratase (cobalt-containing, Co-type NHase; α2β2), Acrylonitrile |
| nitrile hydratase (cobalt-containing, Co-type NHase; α2β2) | Pseudonocardia thermophila | Benzonitrile | 123 | 0.02 | 6.15 × 10³ | pH 7.6, 25 °C | Open record: nitrile hydratase (cobalt-containing, Co-type NHase; α2β2), Benzonitrile |
| nitrile hydratase (cobalt-containing, Co-type NHase; α2β2) | Pseudonocardia thermophila | Methacrylonitrile | 1 × 10³ | 0.49 | 2.04 × 10³ | pH 7.6, 25 °C | Open record: nitrile hydratase (cobalt-containing, Co-type NHase; α2β2), Methacrylonitrile |
| nitrile hydratase (cobalt-containing, Co-type NHase; α2β2)variant αT109S | Pseudonocardia thermophila | Acrylonitrile | 621 | 107 | 5.8 | pH 7.6, 25 °C | Open record: nitrile hydratase (cobalt-containing, Co-type NHase; α2β2) variant αT109S, Acrylonitrile |
| nitrile hydratase (cobalt-containing, Co-type NHase; α2β2)variant αT109S | Pseudonocardia thermophila | Benzonitrile | 132 | 0.025 | 5.29 × 10³ | pH 7.6, 25 °C | Open record: nitrile hydratase (cobalt-containing, Co-type NHase; α2β2) variant αT109S, Benzonitrile |
| nitrile hydratase (cobalt-containing, Co-type NHase; α2β2)variant αT109S | Pseudonocardia thermophila | Methacrylonitrile | 482 | 4.7 | 103 | pH 7.6, 25 °C | Open record: nitrile hydratase (cobalt-containing, Co-type NHase; α2β2) variant αT109S, Methacrylonitrile |
| nitrile hydratase (cobalt-containing, Co-type NHase; α2β2)variant αY114T | Pseudonocardia thermophila | Acrylonitrile | 33.9 | 9.9 | 3.4 | pH 7.6, 25 °C | Open record: nitrile hydratase (cobalt-containing, Co-type NHase; α2β2) variant αY114T, Acrylonitrile |
| nitrile hydratase (cobalt-containing, Co-type NHase; α2β2)variant αY114T | Pseudonocardia thermophila | Benzonitrile | 4.9 | 0.11 | 45 | pH 7.6, 25 °C | Open record: nitrile hydratase (cobalt-containing, Co-type NHase; α2β2) variant αY114T, Benzonitrile |
| nitrile hydratase (cobalt-containing, Co-type NHase; α2β2)variant αY114T | Pseudonocardia thermophila | Methacrylonitrile | 34.6 | 2.3 | 15 | pH 7.6, 25 °C | Open record: nitrile hydratase (cobalt-containing, Co-type NHase; α2β2) variant αY114T, Methacrylonitrile |
| nitrile hydratase (cobalt-containing, Co-type NHase; α2β2)variant βY68F | Pseudonocardia thermophila | Acrylonitrile | 15.2 | 58 | 0.26 | pH 7.6, 25 °C | Open record: nitrile hydratase (cobalt-containing, Co-type NHase; α2β2) variant βY68F, Acrylonitrile |
| nitrile hydratase (cobalt-containing, Co-type NHase; α2β2)variant βY68F | Pseudonocardia thermophila | Benzonitrile | 7.4 | 0.23 | 32 | pH 7.6, 25 °C | Open record: nitrile hydratase (cobalt-containing, Co-type NHase; α2β2) variant βY68F, Benzonitrile |
| nitrile hydratase (cobalt-containing, Co-type NHase; α2β2)variant βY68F | Pseudonocardia thermophila | Methacrylonitrile | 14.1 | 2.6 | 5.4 | pH 7.6, 25 °C | Open record: nitrile hydratase (cobalt-containing, Co-type NHase; α2β2) variant βY68F, Methacrylonitrile |