EC 4.2.1.84: nitrile hydratase
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CatLog read 62 records for EC 4.2.1.84 from 6 papers, for 5 organisms. Names in these papers: nitrile hydratase (cobalt-containing, Co-type NHase; α2β2), nitrile hydratase, low-molecular-mass nitrile hydratase (L-NHase), Co-type nitrile hydratase (ppNHase) and 2 more.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 72 records for EC 4.2.1.84 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 4.2.1.84 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Mutational and structural analysis of cobalt-containing nitrile hydratase on substrate and metal binding | Pseudonocardia thermophila | 14 |
| Characterization of a new cobalt-containing nitrile hydratase purified from urea-induced cells of Rhodococcus rhodochrous J1 | Rhodococcus rhodochrous | 13 |
| doi:10.1016/s0378-1097(98)00447-9 | Rhodococcus rhodochrous | 12 |
| Evidence of the participation of remote residues in the catalytic activity of Co-type nitrile hydratase from Pseudomonas putida | Pseudomonas putida | 11 |
| Analyzing the catalytic role of active site residues in the Fe-type nitrile hydratase from Comamonas testosteroni Ni1 | Comamonas testosteroni | 7 |
| doi:10.1039/c5ra27966f | Ensifer meliloti | 5 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| Co-type nitrile hydratase (ppNHase) | Pseudomonas putida | pentanenitrile | 0.327 | 6.6 | 0.05 | pH 6.7 | PMID 21473592 | Open record: Co-type nitrile hydratase (ppNHase), pentanenitrile |
| Co-type nitrile hydratase (ppNHase)variant α-D164N | Pseudomonas putida | pentanenitrile | 0.0045 | 1.8 | 0.0025 | pH 6.7 | PMID 21473592 | Open record: Co-type nitrile hydratase (ppNHase) variant α-D164N, pentanenitrile |
| Co-type nitrile hydratase (ppNHase)variant α-E168Q | Pseudomonas putida | pentanenitrile | 0.062 | 4.5 | 0.0137 | pH 6.7 | PMID 21473592 | Open record: Co-type nitrile hydratase (ppNHase) variant α-E168Q, pentanenitrile |
| Co-type nitrile hydratase (ppNHase)variant α-R170Q | Pseudomonas putida | pentanenitrile | 0.217 | 8.2 | 0.027 | pH 6.7 | PMID 21473592 | Open record: Co-type nitrile hydratase (ppNHase) variant α-R170Q, pentanenitrile |
| Co-type nitrile hydratase (ppNHase)variant α-Y171F | Pseudomonas putida | pentanenitrile | 0.22 | 9.3 | 0.023 | pH 6.7 | PMID 21473592 | Open record: Co-type nitrile hydratase (ppNHase) variant α-Y171F, pentanenitrile |
| Co-type nitrile hydratase (ppNHase)variant β-E56Q | Pseudomonas putida | pentanenitrile | 0.0035 | 15.3 | 2.2 × 10⁻⁴ | pH 6.7 | PMID 21473592 | Open record: Co-type nitrile hydratase (ppNHase) variant β-E56Q, pentanenitrile |
| Co-type nitrile hydratase (ppNHase)variant β-H147N | Pseudomonas putida | pentanenitrile | 0.0118 | 26.4 | 4.5 × 10⁻⁴ | pH 6.7 | PMID 21473592 | Open record: Co-type nitrile hydratase (ppNHase) variant β-H147N, pentanenitrile |
| Co-type nitrile hydratase (ppNHase)variant β-H71F | Pseudomonas putida | pentanenitrile | 0.027 | 21.2 | 0.00122 | pH 6.7 | PMID 21473592 | Open record: Co-type nitrile hydratase (ppNHase) variant β-H71F, pentanenitrile |
| Co-type nitrile hydratase (ppNHase)variant β-H71L | Pseudomonas putida | pentanenitrile | 0.02 | 10 | 0.002 | pH 6.7 | PMID 21473592 | Open record: Co-type nitrile hydratase (ppNHase) variant β-H71L, pentanenitrile |
| Co-type nitrile hydratase (ppNHase)variant β-H71N | Pseudomonas putida | pentanenitrile | 0.025 | 20.1 | 0.00123 | pH 6.7 | PMID 21473592 | Open record: Co-type nitrile hydratase (ppNHase) variant β-H71N, pentanenitrile |
| Co-type nitrile hydratase (ppNHase)variant β-Y215F | Pseudomonas putida | pentanenitrile | 0.04 | 2.7 | 0.0152 | pH 6.7 | PMID 21473592 | Open record: Co-type nitrile hydratase (ppNHase) variant β-Y215F, pentanenitrile |
| Fe-type nitrile hydratase (CtNHase) | Comamonas testosteroni | acrylonitrile | 1.1 × 10³ | 250 | 4.4 calc. | pH 7.5, 25 °C | PMID 26077812 | Open record: Fe-type nitrile hydratase (CtNHase), acrylonitrile |
| Fe-type nitrile hydratase (CtNHase)variant αH80A | Comamonas testosteroni | acrylonitrile | 220 | 187 | 1.2 calc. | pH 7.5, 25 °C | PMID 26077812 | Open record: Fe-type nitrile hydratase (CtNHase) variant αH80A, acrylonitrile |
| Fe-type nitrile hydratase (CtNHase)variant αH80A/αH81A | Comamonas testosteroni | acrylonitrile | 132 | 213 | 0.620 calc. | pH 7.5, 25 °C | PMID 26077812 | Open record: Fe-type nitrile hydratase (CtNHase) variant αH80A/αH81A, acrylonitrile |
| Fe-type nitrile hydratase (CtNHase)variant αH80W/αH81W | Comamonas testosteroni | acrylonitrile | 79 | 232 | 0.34 calc. | pH 7.5, 25 °C | PMID 26077812 | Open record: Fe-type nitrile hydratase (CtNHase) variant αH80W/αH81W, acrylonitrile |
| Fe-type nitrile hydratase (CtNHase)variant αH81A | Comamonas testosteroni | acrylonitrile | 77 | 179 | 0.43 calc. | pH 7.5, 25 °C | PMID 26077812 | Open record: Fe-type nitrile hydratase (CtNHase) variant αH81A, acrylonitrile |
| Fe-type nitrile hydratase (CtNHase)variant αR157A | Comamonas testosteroni | acrylonitrile | 10 | 204 | 0.049 calc. | pH 7.5, 25 °C | PMID 26077812 | Open record: Fe-type nitrile hydratase (CtNHase) variant αR157A, acrylonitrile |
| Fe-type nitrile hydratase (CtNHase)variant αR157K | Comamonas testosteroni | acrylonitrile | 32 | 239 | 0.13 calc. | pH 7.5, 25 °C | PMID 26077812 | Open record: Fe-type nitrile hydratase (CtNHase) variant αR157K, acrylonitrile |
| low-molecular-mass nitrile hydratase (L-NHase) | Rhodococcus rhodochrous | 2-Cyanopyrazine | — | 0.71 | — | pH 7, 20 °C | doi:10.1016/s0378-1097(98)00447-9 | Open record: low-molecular-mass nitrile hydratase (L-NHase), 2-Cyanopyrazine |
| low-molecular-mass nitrile hydratase (L-NHase) | Rhodococcus rhodochrous | 2-Cyanopyridine | — | 0.56 | — | pH 7, 20 °C | doi:10.1016/s0378-1097(98)00447-9 | Open record: low-molecular-mass nitrile hydratase (L-NHase), 2-Cyanopyridine |
| low-molecular-mass nitrile hydratase (L-NHase) | Rhodococcus rhodochrous | 3-Cyanopyridine | — | 0.3 | — | pH 7, 20 °C | doi:10.1016/s0378-1097(98)00447-9 | Open record: low-molecular-mass nitrile hydratase (L-NHase), 3-Cyanopyridine |
| low-molecular-mass nitrile hydratase (L-NHase) | Rhodococcus rhodochrous | 4-Cyanopyridine | — | 0.28 | — | pH 7, 20 °C | doi:10.1016/s0378-1097(98)00447-9 | Open record: low-molecular-mass nitrile hydratase (L-NHase), 4-Cyanopyridine |
| low-molecular-mass nitrile hydratase (L-NHase) | Rhodococcus rhodochrous | Acrylonitrile | — | 2.67 | — | pH 7, 20 °C | doi:10.1016/s0378-1097(98)00447-9 | Open record: low-molecular-mass nitrile hydratase (L-NHase), Acrylonitrile |
| low-molecular-mass nitrile hydratase (L-NHase) | Rhodococcus rhodochrous | Benzonitrile | — | 0.05 | — | pH 7, 20 °C | doi:10.1016/s0378-1097(98)00447-9 | Open record: low-molecular-mass nitrile hydratase (L-NHase), Benzonitrile |
| low-molecular-mass nitrile hydratase (L-NHase) | Rhodococcus rhodochrous | Chloroacetonitrile | — | 6.25 | — | pH 7, 20 °C | doi:10.1016/s0378-1097(98)00447-9 | Open record: low-molecular-mass nitrile hydratase (L-NHase), Chloroacetonitrile |
| low-molecular-mass nitrile hydratase (L-NHase) | Rhodococcus rhodochrous | Crotononitrile | — | 4.55 | — | pH 7, 20 °C | doi:10.1016/s0378-1097(98)00447-9 | Open record: low-molecular-mass nitrile hydratase (L-NHase), Crotononitrile |
| low-molecular-mass nitrile hydratase (L-NHase) | Rhodococcus rhodochrous | Isobutyronitrile | — | 1.51 | — | pH 7, 20 °C | doi:10.1016/s0378-1097(98)00447-9 | Open record: low-molecular-mass nitrile hydratase (L-NHase), Isobutyronitrile |
| low-molecular-mass nitrile hydratase (L-NHase) | Rhodococcus rhodochrous | Methacrylonitrile | — | 0.44 | — | pH 7, 20 °C | doi:10.1016/s0378-1097(98)00447-9 | Open record: low-molecular-mass nitrile hydratase (L-NHase), Methacrylonitrile |
| low-molecular-mass nitrile hydratase (L-NHase) | Rhodococcus rhodochrous | n-Butyronitrile | — | 0.65 | — | pH 7, 20 °C | doi:10.1016/s0378-1097(98)00447-9 | Open record: low-molecular-mass nitrile hydratase (L-NHase), n-Butyronitrile |
| low-molecular-mass nitrile hydratase (L-NHase) | Rhodococcus rhodochrous | Propionitrile | — | 1.89 | — | pH 7, 20 °C | doi:10.1016/s0378-1097(98)00447-9 | Open record: low-molecular-mass nitrile hydratase (L-NHase), Propionitrile |
| nitrile hydratase | Rhodococcus rhodochrous | 2-Cyanopyridine | — | 21.7 | — | pH 7, 20 °C | PMID 2013281 | Open record: nitrile hydratase, 2-Cyanopyridine |
| nitrile hydratase | Rhodococcus rhodochrous | 3-Cyanopyridine | — | 200 | — | pH 7, 20 °C | PMID 2013281 | Open record: nitrile hydratase, 3-Cyanopyridine |
| nitrile hydratase | Rhodococcus rhodochrous | 4-Cyanopyridine | — | 18.5 | — | pH 7, 20 °C | PMID 2013281 | Open record: nitrile hydratase, 4-Cyanopyridine |
| nitrile hydratase | Rhodococcus rhodochrous | Acrylonitrile | — | 1.89 | — | pH 7, 20 °C | PMID 2013281 | Open record: nitrile hydratase, Acrylonitrile |
| nitrile hydratase | Rhodococcus rhodochrous | Benzonitrile | — | 28.6 | — | pH 7, 20 °C | PMID 2013281 | Open record: nitrile hydratase, Benzonitrile |
| nitrile hydratase | Rhodococcus rhodochrous | Chloroacetonitrile | — | 12.2 | — | pH 7, 20 °C | PMID 2013281 | Open record: nitrile hydratase, Chloroacetonitrile |
| nitrile hydratase | Rhodococcus rhodochrous | Crotononitrile | — | 4.88 | — | pH 7, 20 °C | PMID 2013281 | Open record: nitrile hydratase, Crotononitrile |
| nitrile hydratase | Rhodococcus rhodochrous | Cyanopyrazine | — | 10 | — | pH 7, 20 °C | PMID 2013281 | Open record: nitrile hydratase, Cyanopyrazine |
| nitrile hydratase | Rhodococcus rhodochrous | Isobutyronitrile | — | 1.85 | — | pH 7, 20 °C | PMID 2013281 | Open record: nitrile hydratase, Isobutyronitrile |
| nitrile hydratase | Rhodococcus rhodochrous | Methacrylonitrile | — | 6.76 | — | pH 7, 20 °C | PMID 2013281 | Open record: nitrile hydratase, Methacrylonitrile |
| nitrile hydratase | Rhodococcus rhodochrous | n-Butyronitrile | — | 21.7 | — | pH 7, 20 °C | PMID 2013281 | Open record: nitrile hydratase, n-Butyronitrile |
| nitrile hydratase | Rhodococcus rhodochrous | PropionitrileTable 3 | — | 1.92 | — | pH 7, 20 °C | PMID 2013281 | Open record: nitrile hydratase, Propionitrile |
| nitrile hydratase | Rhodococcus rhodochrous | Propionitrilein the text | 1.347 × 10⁴ | — | — | pH 7, 20 °C | PMID 2013281 | Open record: nitrile hydratase, Propionitrile |
| nitrile hydratase (cobalt-containing, Co-type NHase; α2β2) | Pseudonocardia thermophila | 3-cyanopyridine | 131 | 0.12 | 1.09 × 10³ | pH 7.6, 25 °C | PMID 14717710 | Open record: nitrile hydratase (cobalt-containing, Co-type NHase; α2β2), 3-cyanopyridine |
| nitrile hydratase (cobalt-containing, Co-type NHase; α2β2) | Pseudonocardia thermophila | 4-cyanopyridine | 90 | 0.079 | 1.14 × 10³ | pH 7.6, 25 °C | PMID 14717710 | Open record: nitrile hydratase (cobalt-containing, Co-type NHase; α2β2), 4-cyanopyridine |
| nitrile hydratase (cobalt-containing, Co-type NHase; α2β2) | Pseudonocardia thermophila | Acrylonitrile | 1.91 × 10³ | 3.6 | 537 | pH 7.6, 25 °C | PMID 14717710 | Open record: nitrile hydratase (cobalt-containing, Co-type NHase; α2β2), Acrylonitrile |
| nitrile hydratase (cobalt-containing, Co-type NHase; α2β2) | Pseudonocardia thermophila | Benzonitrile | 123 | 0.02 | 6.15 × 10³ | pH 7.6, 25 °C | PMID 14717710 | Open record: nitrile hydratase (cobalt-containing, Co-type NHase; α2β2), Benzonitrile |
| nitrile hydratase (cobalt-containing, Co-type NHase; α2β2) | Pseudonocardia thermophila | Methacrylonitrile | 1 × 10³ | 0.49 | 2.04 × 10³ | pH 7.6, 25 °C | PMID 14717710 | Open record: nitrile hydratase (cobalt-containing, Co-type NHase; α2β2), Methacrylonitrile |
| nitrile hydratase (cobalt-containing, Co-type NHase; α2β2)variant αT109S | Pseudonocardia thermophila | Acrylonitrile | 621 | 107 | 5.8 | pH 7.6, 25 °C | PMID 14717710 | Open record: nitrile hydratase (cobalt-containing, Co-type NHase; α2β2) variant αT109S, Acrylonitrile |
| nitrile hydratase (cobalt-containing, Co-type NHase; α2β2)variant αT109S | Pseudonocardia thermophila | Benzonitrile | 132 | 0.025 | 5.29 × 10³ | pH 7.6, 25 °C | PMID 14717710 | Open record: nitrile hydratase (cobalt-containing, Co-type NHase; α2β2) variant αT109S, Benzonitrile |
| nitrile hydratase (cobalt-containing, Co-type NHase; α2β2)variant αT109S | Pseudonocardia thermophila | Methacrylonitrile | 482 | 4.7 | 103 | pH 7.6, 25 °C | PMID 14717710 | Open record: nitrile hydratase (cobalt-containing, Co-type NHase; α2β2) variant αT109S, Methacrylonitrile |
| nitrile hydratase (cobalt-containing, Co-type NHase; α2β2)variant αY114T | Pseudonocardia thermophila | Acrylonitrile | 33.9 | 9.9 | 3.4 | pH 7.6, 25 °C | PMID 14717710 | Open record: nitrile hydratase (cobalt-containing, Co-type NHase; α2β2) variant αY114T, Acrylonitrile |
| nitrile hydratase (cobalt-containing, Co-type NHase; α2β2)variant αY114T | Pseudonocardia thermophila | Benzonitrile | 4.9 | 0.11 | 45 | pH 7.6, 25 °C | PMID 14717710 | Open record: nitrile hydratase (cobalt-containing, Co-type NHase; α2β2) variant αY114T, Benzonitrile |
| nitrile hydratase (cobalt-containing, Co-type NHase; α2β2)variant αY114T | Pseudonocardia thermophila | Methacrylonitrile | 34.6 | 2.3 | 15 | pH 7.6, 25 °C | PMID 14717710 | Open record: nitrile hydratase (cobalt-containing, Co-type NHase; α2β2) variant αY114T, Methacrylonitrile |
| nitrile hydratase (cobalt-containing, Co-type NHase; α2β2)variant βY68F | Pseudonocardia thermophila | Acrylonitrile | 15.2 | 58 | 0.26 | pH 7.6, 25 °C | PMID 14717710 | Open record: nitrile hydratase (cobalt-containing, Co-type NHase; α2β2) variant βY68F, Acrylonitrile |
| nitrile hydratase (cobalt-containing, Co-type NHase; α2β2)variant βY68F | Pseudonocardia thermophila | Benzonitrile | 7.4 | 0.23 | 32 | pH 7.6, 25 °C | PMID 14717710 | Open record: nitrile hydratase (cobalt-containing, Co-type NHase; α2β2) variant βY68F, Benzonitrile |
| nitrile hydratase (cobalt-containing, Co-type NHase; α2β2)variant βY68F | Pseudonocardia thermophila | Methacrylonitrile | 14.1 | 2.6 | 5.4 | pH 7.6, 25 °C | PMID 14717710 | Open record: nitrile hydratase (cobalt-containing, Co-type NHase; α2β2) variant βY68F, Methacrylonitrile |
| nitrile hydratase (NHase) | Ensifer meliloti | 3-cyanopyridine | 1055 | 7.34 | 146.4 | pH 7.5, 30 °C | doi:10.1039/c5ra27966f | Open record: nitrile hydratase (NHase), 3-cyanopyridine |
| nitrile hydratase (NHase) | Ensifer meliloti | acetamiprid | 1.04 | 0.14 | 7.42 | pH 7.5, 30 °C | doi:10.1039/c5ra27966f | Open record: nitrile hydratase (NHase), acetamiprid |
| nitrile hydratase (NHase) | Ensifer meliloti | benzonitrile | 1490 | 14.81 | 100.9 | pH 7.5, 30 °C | doi:10.1039/c5ra27966f | Open record: nitrile hydratase (NHase), benzonitrile |
| nitrile hydratase (NHase) | Ensifer meliloti | indole-3-acetonitrile | 426.7 | 17.91 | 23.8 | pH 7.5, 30 °C | doi:10.1039/c5ra27966f | Open record: nitrile hydratase (NHase), indole-3-acetonitrile |
| nitrile hydratase (NHase) | Ensifer meliloti | thiacloprid; (Z)-[3-[(6-chloro-3-pyridinyl)methyl]-2-thiazolidinylidene]cyanamide | 131.4 | 12.39 | 10.6 | pH 7.5, 30 °C | doi:10.1039/c5ra27966f | Open record: nitrile hydratase (NHase), thiacloprid; (Z)-[3-[(6-chloro-3-pyridinyl)methyl]-2-thiazolidinylidene]cyanamide |