CatLogenzyme kinetics catalog

Values CatLog read from this paper

Analyzing the catalytic role of active site residues in the Fe-type nitrile hydratase from Comamonas testosteroni Ni1

PMID 26077812doi:10.1007/s00775-015-1273-3Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 7 records (14 values) from this paper: Fe-type nitrile hydratase (CtNHase) from Comamonas testosteroni.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 7 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 4.2.1.84

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
Fe-type nitrile hydratase (CtNHase)Comamonas testosteroniacrylonitrile1.1 × 10³2504.4 calc.pH 7.5, 25 °COpen
Fe-type nitrile hydratase (CtNHase)variant αH80AComamonas testosteroniacrylonitrile2201871.2 calc.pH 7.5, 25 °COpen
Fe-type nitrile hydratase (CtNHase)variant αH80A/αH81AComamonas testosteroniacrylonitrile1322130.620 calc.pH 7.5, 25 °COpen
Fe-type nitrile hydratase (CtNHase)variant αH80W/αH81WComamonas testosteroniacrylonitrile792320.34 calc.pH 7.5, 25 °COpen
Fe-type nitrile hydratase (CtNHase)variant αH81AComamonas testosteroniacrylonitrile771790.43 calc.pH 7.5, 25 °COpen
Fe-type nitrile hydratase (CtNHase)variant αR157AComamonas testosteroniacrylonitrile102040.049 calc.pH 7.5, 25 °COpen
Fe-type nitrile hydratase (CtNHase)variant αR157KComamonas testosteroniacrylonitrile322390.13 calc.pH 7.5, 25 °COpen