Values CatLog read from this paper
Evidence of the participation of remote residues in the catalytic activity of Co-type nitrile hydratase from Pseudomonas putida
PMID 21473592doi:10.1021/bi101761eOpen in Browse
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CatLog read 11 records (33 values) from this paper: Co-type nitrile hydratase (ppNHase) from Pseudomonas putida.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| Co-type nitrile hydratase (ppNHase) | Pseudomonas putida | pentanenitrile | 0.327 | 6.6 | 0.05 | pH 6.7 | Open record: Co-type nitrile hydratase (ppNHase), pentanenitrile |
| Co-type nitrile hydratase (ppNHase)variant α-D164N | Pseudomonas putida | pentanenitrile | 0.0045 | 1.8 | 0.0025 | pH 6.7 | Open record: Co-type nitrile hydratase (ppNHase) variant α-D164N, pentanenitrile |
| Co-type nitrile hydratase (ppNHase)variant α-E168Q | Pseudomonas putida | pentanenitrile | 0.062 | 4.5 | 0.0137 | pH 6.7 | Open record: Co-type nitrile hydratase (ppNHase) variant α-E168Q, pentanenitrile |
| Co-type nitrile hydratase (ppNHase)variant α-R170Q | Pseudomonas putida | pentanenitrile | 0.217 | 8.2 | 0.027 | pH 6.7 | Open record: Co-type nitrile hydratase (ppNHase) variant α-R170Q, pentanenitrile |
| Co-type nitrile hydratase (ppNHase)variant α-Y171F | Pseudomonas putida | pentanenitrile | 0.22 | 9.3 | 0.023 | pH 6.7 | Open record: Co-type nitrile hydratase (ppNHase) variant α-Y171F, pentanenitrile |
| Co-type nitrile hydratase (ppNHase)variant β-E56Q | Pseudomonas putida | pentanenitrile | 0.0035 | 15.3 | 2.2 × 10⁻⁴ | pH 6.7 | Open record: Co-type nitrile hydratase (ppNHase) variant β-E56Q, pentanenitrile |
| Co-type nitrile hydratase (ppNHase)variant β-H147N | Pseudomonas putida | pentanenitrile | 0.0118 | 26.4 | 4.5 × 10⁻⁴ | pH 6.7 | Open record: Co-type nitrile hydratase (ppNHase) variant β-H147N, pentanenitrile |
| Co-type nitrile hydratase (ppNHase)variant β-H71F | Pseudomonas putida | pentanenitrile | 0.027 | 21.2 | 0.00122 | pH 6.7 | Open record: Co-type nitrile hydratase (ppNHase) variant β-H71F, pentanenitrile |
| Co-type nitrile hydratase (ppNHase)variant β-H71L | Pseudomonas putida | pentanenitrile | 0.02 | 10 | 0.002 | pH 6.7 | Open record: Co-type nitrile hydratase (ppNHase) variant β-H71L, pentanenitrile |
| Co-type nitrile hydratase (ppNHase)variant β-H71N | Pseudomonas putida | pentanenitrile | 0.025 | 20.1 | 0.00123 | pH 6.7 | Open record: Co-type nitrile hydratase (ppNHase) variant β-H71N, pentanenitrile |
| Co-type nitrile hydratase (ppNHase)variant β-Y215F | Pseudomonas putida | pentanenitrile | 0.04 | 2.7 | 0.0152 | pH 6.7 | Open record: Co-type nitrile hydratase (ppNHase) variant β-Y215F, pentanenitrile |