Values CatLog read from this paper
Nucleotide recognition in the ATP-grasp protein carbamoyl phosphate synthetase
PMID 14718657doi:10.1110/ps.03416804Open in Browse
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CatLog read 39 records (116 values) from this paper: carbamoyl phosphate synthetase from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| carbamoyl phosphate synthetase | Escherichia coli | adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; 10 mM glutamine; 0.2 mM NADH | 4.04 | 0.029 | 137.5 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetase | Escherichia coli | adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; no glutamine; 0.2 mM NADH | 0.09 | 0.005 | 17.9 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetase | Escherichia coli | adenosine 5'-triphosphate10 mM carbamoyl phosphate; 20 mM glucose | 0.36 | 0.006 | 65.3 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant A144Q | Escherichia coli | adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; 10 mM glutamine; 0.2 mM NADH | 3.94 | 0.285 | 13.9 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase variant A144Q, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant A144Q | Escherichia coli | adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; no glutamine; 0.2 mM NADH | 0.1 | 0.034 | 2.9 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase variant A144Q, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant A144Q | Escherichia coli | adenosine 5'-triphosphate10 mM carbamoyl phosphate; 20 mM glucose | 0.28 | 0.005 | 56.4 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase variant A144Q, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant A144Q/D207A | Escherichia coli | adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; 10 mM glutamine; 0.2 mM NADH | 1.7 | 0.154 | 11 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase variant A144Q/D207A, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant A144Q/D207A | Escherichia coli | adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; no glutamine; 0.2 mM NADH | 0.12 | 0.021 | 5.8 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase variant A144Q/D207A, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant A144Q/D207A | Escherichia coli | adenosine 5'-triphosphate10 mM carbamoyl phosphate; 20 mM glucose | 0.37 | 0.011 | 33.9 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase variant A144Q/D207A, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant A144Q/D207A/S209A | Escherichia coli | adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; 10 mM glutamine; 0.2 mM NADH | 0.66 | 0.571 | 1.1 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase variant A144Q/D207A/S209A, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant A144Q/D207A/S209A | Escherichia coli | adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; no glutamine; 0.2 mM NADH | 0.33 | 0.319 | 1 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase variant A144Q/D207A/S209A, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant A144Q/D207A/S209A | Escherichia coli | adenosine 5'-triphosphate10 mM carbamoyl phosphate; 20 mM glucose | 0.28 | — | 36.3 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase variant A144Q/D207A/S209A, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant D207A | Escherichia coli | adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; 10 mM glutamine; 0.2 mM NADH | 1.51 | 0.015 | 100.9 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase variant D207A, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant D207A | Escherichia coli | adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; no glutamine; 0.2 mM NADH | 0.11 | 0.006 | 18.9 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase variant D207A, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant D207A | Escherichia coli | adenosine 5'-triphosphate10 mM carbamoyl phosphate; 20 mM glucose | 0.26 | 0.006 | 44.6 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase variant D207A, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant D207N | Escherichia coli | adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; 10 mM glutamine; 0.2 mM NADH | 1.65 | 0.02 | 84.2 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase variant D207N, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant D207N | Escherichia coli | adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; no glutamine; 0.2 mM NADH | 0.06 | 0.009 | 7.2 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase variant D207N, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant D207N | Escherichia coli | adenosine 5'-triphosphate10 mM carbamoyl phosphate; 20 mM glucose | 0.14 | 0.004 | 36.6 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase variant D207N, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant D753A | Escherichia coli | adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; 10 mM glutamine; 0.2 mM NADH | 1.71 | 0.064 | 26.8 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase variant D753A, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant D753A | Escherichia coli | adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; no glutamine; 0.2 mM NADH | 0.14 | 0.007 | 19.4 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase variant D753A, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant D753A | Escherichia coli | adenosine 5'-triphosphate10 mM carbamoyl phosphate; 20 mM glucose | 0.07 | 0.002 | 41.2 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase variant D753A, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant D753N | Escherichia coli | adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; 10 mM glutamine; 0.2 mM NADH | 5.6 | 0.121 | 46.2 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase variant D753N, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant D753N | Escherichia coli | adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; no glutamine; 0.2 mM NADH | 0.13 | 0.008 | 16.9 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase variant D753N, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant D753N | Escherichia coli | adenosine 5'-triphosphate10 mM carbamoyl phosphate; 20 mM glucose | 0.15 | 0.005 | 31.1 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase variant D753N, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant F755A | Escherichia coli | adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; 10 mM glutamine; 0.2 mM NADH | 5.18 | 0.135 | 38.3 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase variant F755A, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant F755A | Escherichia coli | adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; no glutamine; 0.2 mM NADH | 0.07 | 0.004 | 14.8 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase variant F755A, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant F755A | Escherichia coli | adenosine 5'-triphosphate10 mM carbamoyl phosphate; 20 mM glucose | 0.28 | 0.031 | 9 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase variant F755A, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant I211S | Escherichia coli | adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; 10 mM glutamine; 0.2 mM NADH | 4.77 | 0.072 | 66.4 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase variant I211S, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant I211S | Escherichia coli | adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; no glutamine; 0.2 mM NADH | 0.29 | 0.02 | 14.4 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase variant I211S, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant I211S | Escherichia coli | adenosine 5'-triphosphate10 mM carbamoyl phosphate; 20 mM glucose | 0.48 | 0.011 | 44 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase variant I211S, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant P690Q | Escherichia coli | adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; 10 mM glutamine; 0.2 mM NADH | 9.01 | 0.383 | 23.5 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase variant P690Q, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant P690Q | Escherichia coli | adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; no glutamine; 0.2 mM NADH | 0.09 | 0.009 | 10.4 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase variant P690Q, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant P690Q | Escherichia coli | adenosine 5'-triphosphate10 mM carbamoyl phosphate; 20 mM glucose | 0.28 | 0.039 | 7.1 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase variant P690Q, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant P690Q/D753A/F755A | Escherichia coli | adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; 10 mM glutamine; 0.2 mM NADH | 2.9 | 0.199 | 14.6 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase variant P690Q/D753A/F755A, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant P690Q/D753A/F755A | Escherichia coli | adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; no glutamine; 0.2 mM NADH | 0.34 | 0.022 | 15.6 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase variant P690Q/D753A/F755A, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant P690Q/D753A/F755A | Escherichia coli | adenosine 5'-triphosphate10 mM carbamoyl phosphate; 20 mM glucose | 0.06 | 0.027 | 2.3 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase variant P690Q/D753A/F755A, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant S209A | Escherichia coli | adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; 10 mM glutamine; 0.2 mM NADH | 2.43 | 0.031 | 78 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase variant S209A, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant S209A | Escherichia coli | adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; no glutamine; 0.2 mM NADH | 0.35 | 0.016 | 22.7 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase variant S209A, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant S209A | Escherichia coli | adenosine 5'-triphosphate10 mM carbamoyl phosphate; 20 mM glucose | 0.59 | 0.011 | 51.4 | pH 7.6, 25 °C | Open record: carbamoyl phosphate synthetase variant S209A, adenosine 5'-triphosphate |