EC 6.3.5.5: carbamoyl-phosphate synthase (glutamine-hydrolysing)

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CatLog read 181 records for EC 6.3.5.5 from 4 papers, for 1 organism. Names in these papers: carbamoyl phosphate synthetase.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 127 records for EC 6.3.5.5 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 6.3.5.5 in Browse.

Papers

Papers with records for EC 6.3.5.5
Paper	Organisms	Records
Carbamate transport in carbamoyl phosphate synthetase: a theoretical and experimental investigation	Escherichia coli	54
Access to the carbamate tunnel of carbamoyl phosphate synthetase	Escherichia coli	52
Nucleotide recognition in the ATP-grasp protein carbamoyl phosphate synthetase	Escherichia coli	39
Perforation of the tunnel wall in carbamoyl phosphate synthetase derails the passage of ammonia between sequential active sites	Escherichia coli	36

Records

Records for EC 6.3.5.5 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
carbamoyl phosphate synthetase	Escherichia coli	adenosine 5'-diphosphate	0.45	0.016	28 calc.	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase, adenosine 5'-diphosphate
carbamoyl phosphate synthetase	Escherichia coli	adenosine 5'-diphosphate	0.13	0.13	1.0 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase, adenosine 5'-diphosphate
carbamoyl phosphate synthetase	Escherichia coli	adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; 10 mM glutamine; 0.2 mM NADH	4.04	0.029	137.5	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase, adenosine 5'-triphosphate
carbamoyl phosphate synthetase	Escherichia coli	adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; no glutamine; 0.2 mM NADH	0.09	0.005	17.9	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase, adenosine 5'-triphosphate
carbamoyl phosphate synthetase	Escherichia coli	adenosine 5'-triphosphate10 mM carbamoyl phosphate; 20 mM glucose	0.36	0.006	65.3	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase, adenosine 5'-triphosphate
carbamoyl phosphate synthetase	Escherichia coli	adenosine 5'-triphosphate	0.14	—	—	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase, adenosine 5'-triphosphate
carbamoyl phosphate synthetase	Escherichia coli	adenosine 5'-triphosphateGln-dependent ATPase reaction (ADP formation)	3.6	0.24	15 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase, adenosine 5'-triphosphate
carbamoyl phosphate synthetase	Escherichia coli	adenosine 5'-triphosphateKHCO3	0.17	0.051	3.3 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase, adenosine 5'-triphosphate
carbamoyl phosphate synthetase	Escherichia coli	ammoniumNH4+-dependent ATPase reaction (ADP formation); 0.2 mM NADH	3.9	130	0.030 calc.	pH 7.6	PMID 15122899	Open record: carbamoyl phosphate synthetase, ammonium
carbamoyl phosphate synthetase	Escherichia coli	ammonium	1.9	130	0.015 calc.	pH 7.6	PMID 15122899	Open record: carbamoyl phosphate synthetase, ammonium
carbamoyl phosphate synthetase	Escherichia coli	ammonium chloride	3.9	130	0.030 calc.	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase, ammonium chloride
carbamoyl phosphate synthetase	Escherichia coli	L-glutamineglutaminase partial reaction; 5.0 mM ATP	2	—	—	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase, L-glutamine
carbamoyl phosphate synthetase	Escherichia coli	L-glutamineglutamine-dependent ATP hydrolysis; 5.0 mM ATP	4	0.12	33 calc.	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase, L-glutamine
carbamoyl phosphate synthetase	Escherichia coli	L-glutamineoverall carbamoyl phosphate synthesis; 5 mM ATP	1.4	—	—	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase, L-glutamine
carbamoyl phosphate synthetase	Escherichia coli	L-glutamineglutaminase reaction (glutamate formation)	2	0.14	14 calc.	pH 7.6	PMID 15122899	Open record: carbamoyl phosphate synthetase, L-glutamine
carbamoyl phosphate synthetase	Escherichia coli	L-glutamineGln-dependent ATPase reaction (ADP formation); 0.2 mM NADH	3.8	0.1	38 calc.	pH 7.6	PMID 15122899	Open record: carbamoyl phosphate synthetase, L-glutamine
carbamoyl phosphate synthetase	Escherichia coli	L-glutaminevaried glutamine	1.4	0.11	13 calc.	pH 7.6	PMID 15122899	Open record: carbamoyl phosphate synthetase, L-glutamine
carbamoyl phosphate synthetase	Escherichia coli	L-glutamine	0.0016	—	—		PMID 15122899	Open record: carbamoyl phosphate synthetase, L-glutamine
carbamoyl phosphate synthetase	Escherichia coli	L-glutamineglutaminase reaction (glutamate formation); varied glutamine	2.2	0.15	15 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase, L-glutamine
carbamoyl phosphate synthetase	Escherichia coli	L-glutamine	1.9	—	—	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase, L-glutamine
carbamoyl phosphate synthetasevariant A144Q	Escherichia coli	adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; 10 mM glutamine; 0.2 mM NADH	3.94	0.285	13.9	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase variant A144Q, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant A144Q	Escherichia coli	adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; no glutamine; 0.2 mM NADH	0.1	0.034	2.9	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase variant A144Q, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant A144Q	Escherichia coli	adenosine 5'-triphosphate10 mM carbamoyl phosphate; 20 mM glucose	0.28	0.005	56.4	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase variant A144Q, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant A144Q/D207A	Escherichia coli	adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; 10 mM glutamine; 0.2 mM NADH	1.7	0.154	11	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase variant A144Q/D207A, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant A144Q/D207A	Escherichia coli	adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; no glutamine; 0.2 mM NADH	0.12	0.021	5.8	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase variant A144Q/D207A, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant A144Q/D207A	Escherichia coli	adenosine 5'-triphosphate10 mM carbamoyl phosphate; 20 mM glucose	0.37	0.011	33.9	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase variant A144Q/D207A, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant A144Q/D207A/S209A	Escherichia coli	adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; 10 mM glutamine; 0.2 mM NADH	0.66	0.571	1.1	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase variant A144Q/D207A/S209A, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant A144Q/D207A/S209A	Escherichia coli	adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; no glutamine; 0.2 mM NADH	0.33	0.319	1	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase variant A144Q/D207A/S209A, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant A144Q/D207A/S209A	Escherichia coli	adenosine 5'-triphosphate10 mM carbamoyl phosphate; 20 mM glucose	0.28	—	36.3	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase variant A144Q/D207A/S209A, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant A23F	Escherichia coli	adenosine 5'-diphosphate	0.23	0.53	0.43 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant A23F, adenosine 5'-diphosphate
carbamoyl phosphate synthetasevariant A23F	Escherichia coli	adenosine 5'-triphosphateGln-dependent ATPase reaction (ADP formation)	1.3	1.6	0.81 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant A23F, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant A23F	Escherichia coli	adenosine 5'-triphosphateKHCO3	0.097	0.12	0.81 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant A23F, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant A23F	Escherichia coli	L-glutamineglutaminase reaction (glutamate formation); varied glutamine	1.08	0.21	5.1 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant A23F, L-glutamine
carbamoyl phosphate synthetasevariant A23F	Escherichia coli	L-glutamine	0.033	—	—	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant A23F, L-glutamine
carbamoyl phosphate synthetasevariant A23K	Escherichia coli	adenosine 5'-diphosphate	0.32	0.022	15 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant A23K, adenosine 5'-diphosphate
carbamoyl phosphate synthetasevariant A23K	Escherichia coli	adenosine 5'-triphosphateGln-dependent ATPase reaction (ADP formation)	0.28	0.38	0.74 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant A23K, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant A23K	Escherichia coli	adenosine 5'-triphosphateKHCO3	0.17	0.33	0.52 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant A23K, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant A23K	Escherichia coli	L-glutamineglutaminase reaction (glutamate formation); varied glutamine	0.21	0.1	2.1 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant A23K, L-glutamine
carbamoyl phosphate synthetasevariant A23K	Escherichia coli	L-glutamine	0.16	—	—	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant A23K, L-glutamine
carbamoyl phosphate synthetasevariant D207A	Escherichia coli	adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; 10 mM glutamine; 0.2 mM NADH	1.51	0.015	100.9	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase variant D207A, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant D207A	Escherichia coli	adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; no glutamine; 0.2 mM NADH	0.11	0.006	18.9	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase variant D207A, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant D207A	Escherichia coli	adenosine 5'-triphosphate10 mM carbamoyl phosphate; 20 mM glucose	0.26	0.006	44.6	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase variant D207A, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant D207N	Escherichia coli	adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; 10 mM glutamine; 0.2 mM NADH	1.65	0.02	84.2	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase variant D207N, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant D207N	Escherichia coli	adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; no glutamine; 0.2 mM NADH	0.06	0.009	7.2	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase variant D207N, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant D207N	Escherichia coli	adenosine 5'-triphosphate10 mM carbamoyl phosphate; 20 mM glucose	0.14	0.004	36.6	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase variant D207N, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant D753A	Escherichia coli	adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; 10 mM glutamine; 0.2 mM NADH	1.71	0.064	26.8	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase variant D753A, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant D753A	Escherichia coli	adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; no glutamine; 0.2 mM NADH	0.14	0.007	19.4	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase variant D753A, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant D753A	Escherichia coli	adenosine 5'-triphosphate10 mM carbamoyl phosphate; 20 mM glucose	0.07	0.002	41.2	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase variant D753A, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant D753N	Escherichia coli	adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; 10 mM glutamine; 0.2 mM NADH	5.6	0.121	46.2	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase variant D753N, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant D753N	Escherichia coli	adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; no glutamine; 0.2 mM NADH	0.13	0.008	16.9	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase variant D753N, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant D753N	Escherichia coli	adenosine 5'-triphosphate10 mM carbamoyl phosphate; 20 mM glucose	0.15	0.005	31.1	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase variant D753N, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant E25Q/E383Q	Escherichia coli	adenosine 5'-diphosphate	0.091	0.031	2.9 calc.	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E25Q/E383Q, adenosine 5'-diphosphate
carbamoyl phosphate synthetasevariant E25Q/E383Q	Escherichia coli	adenosine 5'-triphosphate	0.31	—	—	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E25Q/E383Q, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant E25Q/E383Q	Escherichia coli	ammonium chloride	0.51	—	—	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E25Q/E383Q, ammonium chloride
carbamoyl phosphate synthetasevariant E25Q/E383Q	Escherichia coli	L-glutamineglutaminase partial reaction; 5.0 mM ATP	0.96	—	—	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E25Q/E383Q, L-glutamine
carbamoyl phosphate synthetasevariant E25Q/E383Q	Escherichia coli	L-glutamineglutamine-dependent ATP hydrolysis; 5.0 mM ATP	0.32	—	—	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E25Q/E383Q, L-glutamine
carbamoyl phosphate synthetasevariant E25Q/E383Q	Escherichia coli	L-glutamineoverall carbamoyl phosphate synthesis; 5 mM ATP	0.03	—	—	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E25Q/E383Q, L-glutamine
carbamoyl phosphate synthetasevariant E25Q/E383Q/E604Q	Escherichia coli	adenosine 5'-diphosphate	0.19	0.022	8.6 calc.	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E25Q/E383Q/E604Q, adenosine 5'-diphosphate
carbamoyl phosphate synthetasevariant E25Q/E383Q/E604Q	Escherichia coli	adenosine 5'-triphosphate	0.061	—	—	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E25Q/E383Q/E604Q, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant E25Q/E383Q/E604Q	Escherichia coli	ammonium chloride	0.097	—	—	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E25Q/E383Q/E604Q, ammonium chloride
carbamoyl phosphate synthetasevariant E25Q/E383Q/E604Q	Escherichia coli	L-glutamineglutaminase partial reaction	0.42	—	—	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E25Q/E383Q/E604Q, L-glutamine
carbamoyl phosphate synthetasevariant E25Q/E383Q/E604Q	Escherichia coli	L-glutamineglutamine-dependent ATP hydrolysis	0.15	—	—	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E25Q/E383Q/E604Q, L-glutamine
carbamoyl phosphate synthetasevariant E383Q	Escherichia coli	adenosine 5'-diphosphate	0.28	0.033	8.5 calc.	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E383Q, adenosine 5'-diphosphate
carbamoyl phosphate synthetasevariant E383Q	Escherichia coli	adenosine 5'-triphosphate	0.62	—	—	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E383Q, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant E383Q	Escherichia coli	ammonium chloride	1.7	110	0.015 calc.	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E383Q, ammonium chloride
carbamoyl phosphate synthetasevariant E383Q	Escherichia coli	L-glutamineglutaminase partial reaction; 5.0 mM ATP	0.84	—	—	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E383Q, L-glutamine
carbamoyl phosphate synthetasevariant E383Q	Escherichia coli	L-glutamineglutamine-dependent ATP hydrolysis; 5.0 mM ATP	3.2	0.15	21 calc.	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E383Q, L-glutamine
carbamoyl phosphate synthetasevariant E383Q	Escherichia coli	L-glutamineoverall carbamoyl phosphate synthesis; 5 mM ATP	1.3	—	—	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E383Q, L-glutamine
carbamoyl phosphate synthetasevariant E383Q/E916Q	Escherichia coli	adenosine 5'-diphosphate	0.39	0.11	3.5 calc.	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E383Q/E916Q, adenosine 5'-diphosphate
carbamoyl phosphate synthetasevariant E383Q/E916Q	Escherichia coli	adenosine 5'-triphosphate	0.27	—	—	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E383Q/E916Q, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant E383Q/E916Q	Escherichia coli	L-glutamineglutaminase partial reaction; 5.0 mM ATP	1.5	—	—	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E383Q/E916Q, L-glutamine
carbamoyl phosphate synthetasevariant E383Q/E916Q	Escherichia coli	L-glutamineglutamine-dependent ATP hydrolysis; 5.0 mM ATP	4	0.14	29 calc.	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E383Q/E916Q, L-glutamine
carbamoyl phosphate synthetasevariant E383Q/E916Q	Escherichia coli	L-glutamineoverall carbamoyl phosphate synthesis; 5 mM ATP	0.36	—	—	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E383Q/E916Q, L-glutamine
carbamoyl phosphate synthetasevariant E577Q	Escherichia coli	adenosine 5'-diphosphate	0.036	0.05	0.72 calc.	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E577Q, adenosine 5'-diphosphate
carbamoyl phosphate synthetasevariant E577Q	Escherichia coli	adenosine 5'-triphosphate	0.12	—	—	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E577Q, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant E577Q	Escherichia coli	ammonium chloride	0.12	—	—	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E577Q, ammonium chloride
carbamoyl phosphate synthetasevariant E577Q	Escherichia coli	L-glutamineglutaminase partial reaction; 5.0 mM ATP	0.091	—	—	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E577Q, L-glutamine
carbamoyl phosphate synthetasevariant E577Q	Escherichia coli	L-glutamineglutamine-dependent ATP hydrolysis; 5.0 mM ATP	0.17	—	—	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E577Q, L-glutamine
carbamoyl phosphate synthetasevariant E577Q	Escherichia coli	L-glutamineoverall carbamoyl phosphate synthesis; 5 mM ATP	0.0032	—	—	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E577Q, L-glutamine
carbamoyl phosphate synthetasevariant E604Q	Escherichia coli	adenosine 5'-diphosphate	0.21	0.065	3.2 calc.	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E604Q, adenosine 5'-diphosphate
carbamoyl phosphate synthetasevariant E604Q	Escherichia coli	adenosine 5'-triphosphate	1.3	—	—	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E604Q, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant E604Q	Escherichia coli	ammonium chloride	1.3	—	—	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E604Q, ammonium chloride
carbamoyl phosphate synthetasevariant E604Q	Escherichia coli	L-glutamineglutaminase partial reaction; 5.0 mM ATP	0.81	—	—	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E604Q, L-glutamine
carbamoyl phosphate synthetasevariant E604Q	Escherichia coli	L-glutamineglutamine-dependent ATP hydrolysis; 5.0 mM ATP	1.5	—	—	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E604Q, L-glutamine
carbamoyl phosphate synthetasevariant E604Q	Escherichia coli	L-glutamineoverall carbamoyl phosphate synthesis; 5 mM ATP	0.38	—	—	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E604Q, L-glutamine
carbamoyl phosphate synthetasevariant E916Q	Escherichia coli	adenosine 5'-diphosphate	0.14	0.017	8.2 calc.	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E916Q, adenosine 5'-diphosphate
carbamoyl phosphate synthetasevariant E916Q	Escherichia coli	adenosine 5'-triphosphate	0.6	—	—	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E916Q, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant E916Q	Escherichia coli	ammonium chloride	1.3	160	0.0081 calc.	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E916Q, ammonium chloride
carbamoyl phosphate synthetasevariant E916Q	Escherichia coli	L-glutamineglutaminase partial reaction; 5.0 mM ATP	1.5	—	—	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E916Q, L-glutamine
carbamoyl phosphate synthetasevariant E916Q	Escherichia coli	L-glutamineglutamine-dependent ATP hydrolysis; 5.0 mM ATP	4.2	0.11	38 calc.	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E916Q, L-glutamine
carbamoyl phosphate synthetasevariant E916Q	Escherichia coli	L-glutamineoverall carbamoyl phosphate synthesis; 5 mM ATP	0.21	—	—	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant E916Q, L-glutamine
carbamoyl phosphate synthetasevariant F755A	Escherichia coli	adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; 10 mM glutamine; 0.2 mM NADH	5.18	0.135	38.3	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase variant F755A, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant F755A	Escherichia coli	adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; no glutamine; 0.2 mM NADH	0.07	0.004	14.8	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase variant F755A, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant F755A	Escherichia coli	adenosine 5'-triphosphate10 mM carbamoyl phosphate; 20 mM glucose	0.28	0.031	9	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase variant F755A, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant G575F	Escherichia coli	adenosine 5'-diphosphate	0.058	0.03	1.9 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant G575F, adenosine 5'-diphosphate
carbamoyl phosphate synthetasevariant G575F	Escherichia coli	adenosine 5'-triphosphateGln-dependent ATPase reaction (ADP formation)	3.3	0.014	240 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant G575F, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant G575F	Escherichia coli	adenosine 5'-triphosphateKHCO3	0.19	0.014	14 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant G575F, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant G575F	Escherichia coli	L-glutamineglutaminase reaction (glutamate formation); varied glutamine	3	0.19	16 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant G575F, L-glutamine
carbamoyl phosphate synthetasevariant G575F	Escherichia coli	L-glutamine	0.072	—	—	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant G575F, L-glutamine
carbamoyl phosphate synthetasevariant G575K	Escherichia coli	adenosine 5'-diphosphate	0.15	0.054	2.8 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant G575K, adenosine 5'-diphosphate
carbamoyl phosphate synthetasevariant G575K	Escherichia coli	adenosine 5'-triphosphateGln-dependent ATPase reaction (ADP formation)	3.3	0.07	47 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant G575K, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant G575K	Escherichia coli	adenosine 5'-triphosphateKHCO3	0.18	0.013	14 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant G575K, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant G575K	Escherichia coli	L-glutamineglutaminase reaction (glutamate formation); varied glutamine	3.1	0.17	18 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant G575K, L-glutamine
carbamoyl phosphate synthetasevariant G575K	Escherichia coli	L-glutamine	1.9	—	—	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant G575K, L-glutamine
carbamoyl phosphate synthetasevariant I18W/A23F/C24F	Escherichia coli	adenosine 5'-diphosphate	0.086	0.18	0.48 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant I18W/A23F/C24F, adenosine 5'-diphosphate
carbamoyl phosphate synthetasevariant I18W/A23F/C24F	Escherichia coli	adenosine 5'-triphosphateGln-dependent ATPase reaction (ADP formation)	0.06	0.25	0.24 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant I18W/A23F/C24F, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant I18W/A23F/C24F	Escherichia coli	adenosine 5'-triphosphateKHCO3	0.039	0.21	0.19 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant I18W/A23F/C24F, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant I18W/A23F/C24F	Escherichia coli	L-glutamine	0.13	0.16	0.81 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant I18W/A23F/C24F, L-glutamine
carbamoyl phosphate synthetasevariant I211S	Escherichia coli	adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; 10 mM glutamine; 0.2 mM NADH	4.77	0.072	66.4	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase variant I211S, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant I211S	Escherichia coli	adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; no glutamine; 0.2 mM NADH	0.29	0.02	14.4	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase variant I211S, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant I211S	Escherichia coli	adenosine 5'-triphosphate10 mM carbamoyl phosphate; 20 mM glucose	0.48	0.011	44	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase variant I211S, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant L648E	Escherichia coli	adenosine 5'-diphosphate	0.024	0.34	0.071 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant L648E, adenosine 5'-diphosphate
carbamoyl phosphate synthetasevariant L648E	Escherichia coli	adenosine 5'-triphosphateGln-dependent ATPase reaction (ADP formation)	3.2	1.2	2.7 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant L648E, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant L648E	Escherichia coli	adenosine 5'-triphosphateKHCO3	0.59	0.5	1.2 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant L648E, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant L648E	Escherichia coli	L-glutamine	1.3	0.23	5.7 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant L648E, L-glutamine
carbamoyl phosphate synthetasevariant L720E	Escherichia coli	adenosine 5'-diphosphate	0.031	0.42	0.074 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant L720E, adenosine 5'-diphosphate
carbamoyl phosphate synthetasevariant L720E	Escherichia coli	adenosine 5'-triphosphateGln-dependent ATPase reaction (ADP formation)	2	1.7	1.2 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant L720E, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant L720E	Escherichia coli	adenosine 5'-triphosphateKHCO3	0.39	0.22	1.8 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant L720E, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant L720E	Escherichia coli	L-glutamine	0.84	0.18	4.7 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant L720E, L-glutamine
carbamoyl phosphate synthetasevariant M174E	Escherichia coli	adenosine 5'-diphosphate	0.1	0.088	1.1 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant M174E, adenosine 5'-diphosphate
carbamoyl phosphate synthetasevariant M174E	Escherichia coli	adenosine 5'-triphosphateGln-dependent ATPase reaction (ADP formation)	0.11	0.69	0.16 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant M174E, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant M174E	Escherichia coli	adenosine 5'-triphosphateKHCO3	0.053	0.36	0.15 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant M174E, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant M174E	Escherichia coli	L-glutamine	0.15	0.39	0.38 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant M174E, L-glutamine
carbamoyl phosphate synthetasevariant M174E/M378E	Escherichia coli	adenosine 5'-diphosphate	0.22	0.17	1.3 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant M174E/M378E, adenosine 5'-diphosphate
carbamoyl phosphate synthetasevariant M174E/M378E	Escherichia coli	adenosine 5'-triphosphateGln-dependent ATPase reaction (ADP formation)	0.043	0.28	0.15 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant M174E/M378E, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant M174E/M378E	Escherichia coli	adenosine 5'-triphosphateKHCO3	0.028	0.29	0.097 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant M174E/M378E, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant M174E/M378E	Escherichia coli	L-glutamine	0.1	0.47	0.21 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant M174E/M378E, L-glutamine
carbamoyl phosphate synthetasevariant M378E	Escherichia coli	adenosine 5'-diphosphate	0.2	0.13	1.5 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant M378E, adenosine 5'-diphosphate
carbamoyl phosphate synthetasevariant M378E	Escherichia coli	adenosine 5'-triphosphateGln-dependent ATPase reaction (ADP formation)	0.035	0.16	0.22 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant M378E, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant M378E	Escherichia coli	adenosine 5'-triphosphateKHCO3	0.013	0.03	0.43 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant M378E, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant M378E	Escherichia coli	L-glutamineglutaminase reaction (glutamate formation); varied glutamine	0.13	0.35	0.37 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant M378E, L-glutamine
carbamoyl phosphate synthetasevariant M378E	Escherichia coli	L-glutamine	0.012	—	—	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant M378E, L-glutamine
carbamoyl phosphate synthetasevariant M911E	Escherichia coli	adenosine 5'-triphosphateGln-dependent ATPase reaction (ADP formation)	0.5	1	0.50 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant M911E, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant M911E	Escherichia coli	adenosine 5'-triphosphateKHCO3	0.33	0.26	1.3 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant M911E, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant M911E	Escherichia coli	L-glutamineglutaminase reaction (glutamate formation); varied glutamine	0.14	0.18	0.78 calc.	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant M911E, L-glutamine
carbamoyl phosphate synthetasevariant M911E	Escherichia coli	L-glutamine	0.015	—	—	pH 7.6	PMID 20187643	Open record: carbamoyl phosphate synthetase variant M911E, L-glutamine
carbamoyl phosphate synthetasevariant P690Q	Escherichia coli	adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; 10 mM glutamine; 0.2 mM NADH	9.01	0.383	23.5	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase variant P690Q, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant P690Q	Escherichia coli	adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; no glutamine; 0.2 mM NADH	0.09	0.009	10.4	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase variant P690Q, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant P690Q	Escherichia coli	adenosine 5'-triphosphate10 mM carbamoyl phosphate; 20 mM glucose	0.28	0.039	7.1	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase variant P690Q, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant P690Q/D753A/F755A	Escherichia coli	adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; 10 mM glutamine; 0.2 mM NADH	2.9	0.199	14.6	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase variant P690Q/D753A/F755A, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant P690Q/D753A/F755A	Escherichia coli	adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; no glutamine; 0.2 mM NADH	0.34	0.022	15.6	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase variant P690Q/D753A/F755A, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant P690Q/D753A/F755A	Escherichia coli	adenosine 5'-triphosphate10 mM carbamoyl phosphate; 20 mM glucose	0.06	0.027	2.3	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase variant P690Q/D753A/F755A, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant R306A	Escherichia coli	adenosine 5'-triphosphatecosubstrate bicarbonate	0.086	—	—	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant R306A, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant R306A	Escherichia coli	adenosine 5'-triphosphateglutamine-dependent ATP hydrolysis; 10 mM glutamine	0.1	0.22	0.45 calc.	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant R306A, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant R848A	Escherichia coli	adenosine 5'-diphosphate	0.09	6.9	0.013 calc.	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant R848A, adenosine 5'-diphosphate
carbamoyl phosphate synthetasevariant R848A	Escherichia coli	adenosine 5'-triphosphatecosubstrate bicarbonate	0.6	—	—	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant R848A, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant R848A	Escherichia coli	adenosine 5'-triphosphateglutamine-dependent ATP hydrolysis; 10 mM glutamine	0.95	0.4	2.4 calc.	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant R848A, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant R848A	Escherichia coli	L-glutamine	0.006	—	—	pH 7.6	PMID 15081891	Open record: carbamoyl phosphate synthetase variant R848A, L-glutamine
carbamoyl phosphate synthetasevariant S209A	Escherichia coli	adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; 10 mM glutamine; 0.2 mM NADH	2.43	0.031	78	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase variant S209A, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant S209A	Escherichia coli	adenosine 5'-triphosphate40 mM NaHCO3; 1 mM DTT; no glutamine; 0.2 mM NADH	0.35	0.016	22.7	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase variant S209A, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant S209A	Escherichia coli	adenosine 5'-triphosphate10 mM carbamoyl phosphate; 20 mM glucose	0.59	0.011	51.4	pH 7.6, 25 °C	PMID 14718657	Open record: carbamoyl phosphate synthetase variant S209A, adenosine 5'-triphosphate
carbamoyl phosphate synthetasevariant αD362A	Escherichia coli	ammoniumNH4+-dependent ATPase reaction (ADP formation); 0.2 mM NADH	9.5	120	0.079 calc.	pH 7.6	PMID 15122899	Open record: carbamoyl phosphate synthetase variant αD362A, ammonium
carbamoyl phosphate synthetasevariant αD362A	Escherichia coli	ammonium	3.8	24	0.16 calc.	pH 7.6	PMID 15122899	Open record: carbamoyl phosphate synthetase variant αD362A, ammonium
carbamoyl phosphate synthetasevariant αD362A	Escherichia coli	L-glutamineglutaminase reaction (glutamate formation)	2.1	0.092	23 calc.	pH 7.6	PMID 15122899	Open record: carbamoyl phosphate synthetase variant αD362A, L-glutamine
carbamoyl phosphate synthetasevariant αD362A	Escherichia coli	L-glutamineGln-dependent ATPase reaction (ADP formation); 0.2 mM NADH	4.7	0.066	71 calc.	pH 7.6	PMID 15122899	Open record: carbamoyl phosphate synthetase variant αD362A, L-glutamine
carbamoyl phosphate synthetasevariant αD362A	Escherichia coli	L-glutaminevaried glutamine	2.4	0.19	13 calc.	pH 7.6	PMID 15122899	Open record: carbamoyl phosphate synthetase variant αD362A, L-glutamine
carbamoyl phosphate synthetasevariant αD362A/βR265A	Escherichia coli	ammoniumNH4+-dependent ATPase reaction (ADP formation); 0.2 mM NADH	10.3	55	0.19 calc.	pH 7.6	PMID 15122899	Open record: carbamoyl phosphate synthetase variant αD362A/βR265A, ammonium
carbamoyl phosphate synthetasevariant αD362A/βR265A	Escherichia coli	ammonium	5.8	59	0.098 calc.	pH 7.6	PMID 15122899	Open record: carbamoyl phosphate synthetase variant αD362A/βR265A, ammonium
carbamoyl phosphate synthetasevariant αD362A/βR265A	Escherichia coli	L-glutamineglutaminase reaction (glutamate formation)	3.2	0.38	8.4 calc.	pH 7.6	PMID 15122899	Open record: carbamoyl phosphate synthetase variant αD362A/βR265A, L-glutamine
carbamoyl phosphate synthetasevariant αD362A/βR265A	Escherichia coli	L-glutamineGln-dependent ATPase reaction (ADP formation); 0.2 mM NADH	2.3	5.5	0.42 calc.	pH 7.6	PMID 15122899	Open record: carbamoyl phosphate synthetase variant αD362A/βR265A, L-glutamine
carbamoyl phosphate synthetasevariant αD362A/βR265A	Escherichia coli	L-glutaminevaried glutamine	1.2	7.8	0.15 calc.	pH 7.6	PMID 15122899	Open record: carbamoyl phosphate synthetase variant αD362A/βR265A, L-glutamine
carbamoyl phosphate synthetasevariant αP360A/αH361A	Escherichia coli	L-glutamineglutaminase reaction (glutamate formation)	3.4	0.39	8.7 calc.	pH 7.6	PMID 15122899	Open record: carbamoyl phosphate synthetase variant αP360A/αH361A, L-glutamine
carbamoyl phosphate synthetasevariant αP360A/αH361A	Escherichia coli	L-glutamineGln-dependent ATPase reaction (ADP formation); 0.2 mM NADH	6	0.4	15 calc.	pH 7.6	PMID 15122899	Open record: carbamoyl phosphate synthetase variant αP360A/αH361A, L-glutamine
carbamoyl phosphate synthetasevariant αP360A/αH361A	Escherichia coli	L-glutaminevaried glutamine	2.3	0.36	6.4 calc.	pH 7.6	PMID 15122899	Open record: carbamoyl phosphate synthetase variant αP360A/αH361A, L-glutamine
carbamoyl phosphate synthetasevariant αP360A/αH361A/βR265A	Escherichia coli	ammonia	—	—	0.208	pH 7.6	PMID 15122899	Open record: carbamoyl phosphate synthetase variant αP360A/αH361A/βR265A, ammonia
carbamoyl phosphate synthetasevariant αP360A/αH361A/βR265A	Escherichia coli	ammoniumNH4+-dependent ATPase reaction (ADP formation); 0.2 mM NADH	4.7	59	0.080 calc.	pH 7.6	PMID 15122899	Open record: carbamoyl phosphate synthetase variant αP360A/αH361A/βR265A, ammonium
carbamoyl phosphate synthetasevariant αP360A/αH361A/βR265A	Escherichia coli	ammonium	2.5	12	0.21 calc.	pH 7.6	PMID 15122899	Open record: carbamoyl phosphate synthetase variant αP360A/αH361A/βR265A, ammonium
carbamoyl phosphate synthetasevariant βQ262A/βR265A	Escherichia coli	ammoniumNH4+-dependent ATPase reaction (ADP formation); 0.2 mM NADH	5	58	0.086 calc.	pH 7.6	PMID 15122899	Open record: carbamoyl phosphate synthetase variant βQ262A/βR265A, ammonium
carbamoyl phosphate synthetasevariant βQ262A/βR265A	Escherichia coli	ammonium	3.6	26	0.14 calc.	pH 7.6	PMID 15122899	Open record: carbamoyl phosphate synthetase variant βQ262A/βR265A, ammonium
carbamoyl phosphate synthetasevariant βQ262A/βR265A	Escherichia coli	L-glutamineglutaminase reaction (glutamate formation)	2.6	6.4	0.41 calc.	pH 7.6	PMID 15122899	Open record: carbamoyl phosphate synthetase variant βQ262A/βR265A, L-glutamine
carbamoyl phosphate synthetasevariant βQ262A/βR265A	Escherichia coli	L-glutamineGln-dependent ATPase reaction (ADP formation); 0.2 mM NADH	2.9	4.1	0.71 calc.	pH 7.6	PMID 15122899	Open record: carbamoyl phosphate synthetase variant βQ262A/βR265A, L-glutamine
carbamoyl phosphate synthetasevariant βQ262A/βR265A	Escherichia coli	L-glutaminevaried glutamine	1	4.7	0.21 calc.	pH 7.6	PMID 15122899	Open record: carbamoyl phosphate synthetase variant βQ262A/βR265A, L-glutamine
carbamoyl phosphate synthetasevariant βQ262A/βR265A/βN266A	Escherichia coli	ammonium	6.7	56	0.12 calc.	pH 7.6	PMID 15122899	Open record: carbamoyl phosphate synthetase variant βQ262A/βR265A/βN266A, ammonium
carbamoyl phosphate synthetasevariant βQ262A/βR265A/βN266A	Escherichia coli	L-glutamineglutaminase reaction (glutamate formation)	1.7	5.5	0.31 calc.	pH 7.6	PMID 15122899	Open record: carbamoyl phosphate synthetase variant βQ262A/βR265A/βN266A, L-glutamine
carbamoyl phosphate synthetasevariant βQ262A/βR265A/βN266A	Escherichia coli	L-glutamineGln-dependent ATPase reaction (ADP formation); 0.2 mM NADH	1.9	1.9	1.0 calc.	pH 7.6	PMID 15122899	Open record: carbamoyl phosphate synthetase variant βQ262A/βR265A/βN266A, L-glutamine
carbamoyl phosphate synthetasevariant βQ262A/βR265A/βN266A	Escherichia coli	L-glutaminevaried glutamine	0.88	14.9	0.059 calc.	pH 7.6	PMID 15122899	Open record: carbamoyl phosphate synthetase variant βQ262A/βR265A/βN266A, L-glutamine
carbamoyl phosphate synthetasevariant βR265A	Escherichia coli	ammoniumNH4+-dependent ATPase reaction (ADP formation); 0.2 mM NADH	5.7	41	0.14 calc.	pH 7.6	PMID 15122899	Open record: carbamoyl phosphate synthetase variant βR265A, ammonium
carbamoyl phosphate synthetasevariant βR265A	Escherichia coli	ammonium	2.5	26	0.096 calc.	pH 7.6	PMID 15122899	Open record: carbamoyl phosphate synthetase variant βR265A, ammonium
carbamoyl phosphate synthetasevariant βR265A	Escherichia coli	L-glutamineglutaminase reaction (glutamate formation)	3.7	0.36	10 calc.	pH 7.6	PMID 15122899	Open record: carbamoyl phosphate synthetase variant βR265A, L-glutamine
carbamoyl phosphate synthetasevariant βR265A	Escherichia coli	L-glutamineGln-dependent ATPase reaction (ADP formation); 0.2 mM NADH	2.8	6.4	0.44 calc.	pH 7.6	PMID 15122899	Open record: carbamoyl phosphate synthetase variant βR265A, L-glutamine
carbamoyl phosphate synthetasevariant βR265A	Escherichia coli	L-glutaminevaried glutamine	1.1	7.6	0.14 calc.	pH 7.6	PMID 15122899	Open record: carbamoyl phosphate synthetase variant βR265A, L-glutamine