Values CatLog read from this paper
Carbamate transport in carbamoyl phosphate synthetase: a theoretical and experimental investigation
PMID 20187643doi:10.1021/ja910441vOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 54 records (101 values) from this paper: carbamoyl phosphate synthetase from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 47 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| carbamoyl phosphate synthetase | Escherichia coli | adenosine 5'-diphosphate | 0.13 | 0.13 | 1.0 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase, adenosine 5'-diphosphate |
| carbamoyl phosphate synthetase | Escherichia coli | adenosine 5'-triphosphateGln-dependent ATPase reaction (ADP formation) | 3.6 | 0.24 | 15 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetase | Escherichia coli | adenosine 5'-triphosphateKHCO3 | 0.17 | 0.051 | 3.3 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetase | Escherichia coli | L-glutamineglutaminase reaction (glutamate formation); varied glutamine | 2.2 | 0.15 | 15 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase, L-glutamine |
| carbamoyl phosphate synthetase | Escherichia coli | L-glutamine | 1.9 | — | — | pH 7.6 | Open record: carbamoyl phosphate synthetase, L-glutamine |
| carbamoyl phosphate synthetasevariant A23F | Escherichia coli | adenosine 5'-diphosphate | 0.23 | 0.53 | 0.43 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant A23F, adenosine 5'-diphosphate |
| carbamoyl phosphate synthetasevariant A23F | Escherichia coli | adenosine 5'-triphosphateGln-dependent ATPase reaction (ADP formation) | 1.3 | 1.6 | 0.81 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant A23F, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant A23F | Escherichia coli | adenosine 5'-triphosphateKHCO3 | 0.097 | 0.12 | 0.81 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant A23F, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant A23F | Escherichia coli | L-glutamineglutaminase reaction (glutamate formation); varied glutamine | 1.08 | 0.21 | 5.1 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant A23F, L-glutamine |
| carbamoyl phosphate synthetasevariant A23F | Escherichia coli | L-glutamine | 0.033 | — | — | pH 7.6 | Open record: carbamoyl phosphate synthetase variant A23F, L-glutamine |
| carbamoyl phosphate synthetasevariant A23K | Escherichia coli | adenosine 5'-diphosphate | 0.32 | 0.022 | 15 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant A23K, adenosine 5'-diphosphate |
| carbamoyl phosphate synthetasevariant A23K | Escherichia coli | adenosine 5'-triphosphateGln-dependent ATPase reaction (ADP formation) | 0.28 | 0.38 | 0.74 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant A23K, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant A23K | Escherichia coli | adenosine 5'-triphosphateKHCO3 | 0.17 | 0.33 | 0.52 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant A23K, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant A23K | Escherichia coli | L-glutamineglutaminase reaction (glutamate formation); varied glutamine | 0.21 | 0.1 | 2.1 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant A23K, L-glutamine |
| carbamoyl phosphate synthetasevariant A23K | Escherichia coli | L-glutamine | 0.16 | — | — | pH 7.6 | Open record: carbamoyl phosphate synthetase variant A23K, L-glutamine |
| carbamoyl phosphate synthetasevariant G575F | Escherichia coli | adenosine 5'-diphosphate | 0.058 | 0.03 | 1.9 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant G575F, adenosine 5'-diphosphate |
| carbamoyl phosphate synthetasevariant G575F | Escherichia coli | adenosine 5'-triphosphateGln-dependent ATPase reaction (ADP formation) | 3.3 | 0.014 | 240 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant G575F, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant G575F | Escherichia coli | adenosine 5'-triphosphateKHCO3 | 0.19 | 0.014 | 14 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant G575F, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant G575F | Escherichia coli | L-glutamineglutaminase reaction (glutamate formation); varied glutamine | 3 | 0.19 | 16 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant G575F, L-glutamine |
| carbamoyl phosphate synthetasevariant G575F | Escherichia coli | L-glutamine | 0.072 | — | — | pH 7.6 | Open record: carbamoyl phosphate synthetase variant G575F, L-glutamine |
| carbamoyl phosphate synthetasevariant G575K | Escherichia coli | adenosine 5'-diphosphate | 0.15 | 0.054 | 2.8 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant G575K, adenosine 5'-diphosphate |
| carbamoyl phosphate synthetasevariant G575K | Escherichia coli | adenosine 5'-triphosphateGln-dependent ATPase reaction (ADP formation) | 3.3 | 0.07 | 47 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant G575K, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant G575K | Escherichia coli | adenosine 5'-triphosphateKHCO3 | 0.18 | 0.013 | 14 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant G575K, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant G575K | Escherichia coli | L-glutamineglutaminase reaction (glutamate formation); varied glutamine | 3.1 | 0.17 | 18 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant G575K, L-glutamine |
| carbamoyl phosphate synthetasevariant G575K | Escherichia coli | L-glutamine | 1.9 | — | — | pH 7.6 | Open record: carbamoyl phosphate synthetase variant G575K, L-glutamine |
| carbamoyl phosphate synthetasevariant I18W/A23F/C24F | Escherichia coli | adenosine 5'-diphosphate | 0.086 | 0.18 | 0.48 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant I18W/A23F/C24F, adenosine 5'-diphosphate |
| carbamoyl phosphate synthetasevariant I18W/A23F/C24F | Escherichia coli | adenosine 5'-triphosphateGln-dependent ATPase reaction (ADP formation) | 0.06 | 0.25 | 0.24 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant I18W/A23F/C24F, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant I18W/A23F/C24F | Escherichia coli | adenosine 5'-triphosphateKHCO3 | 0.039 | 0.21 | 0.19 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant I18W/A23F/C24F, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant I18W/A23F/C24F | Escherichia coli | L-glutamine | 0.13 | 0.16 | 0.81 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant I18W/A23F/C24F, L-glutamine |
| carbamoyl phosphate synthetasevariant L648E | Escherichia coli | adenosine 5'-diphosphate | 0.024 | 0.34 | 0.071 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant L648E, adenosine 5'-diphosphate |
| carbamoyl phosphate synthetasevariant L648E | Escherichia coli | adenosine 5'-triphosphateGln-dependent ATPase reaction (ADP formation) | 3.2 | 1.2 | 2.7 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant L648E, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant L648E | Escherichia coli | adenosine 5'-triphosphateKHCO3 | 0.59 | 0.5 | 1.2 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant L648E, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant L648E | Escherichia coli | L-glutamine | 1.3 | 0.23 | 5.7 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant L648E, L-glutamine |
| carbamoyl phosphate synthetasevariant L720E | Escherichia coli | adenosine 5'-diphosphate | 0.031 | 0.42 | 0.074 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant L720E, adenosine 5'-diphosphate |
| carbamoyl phosphate synthetasevariant L720E | Escherichia coli | adenosine 5'-triphosphateGln-dependent ATPase reaction (ADP formation) | 2 | 1.7 | 1.2 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant L720E, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant L720E | Escherichia coli | adenosine 5'-triphosphateKHCO3 | 0.39 | 0.22 | 1.8 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant L720E, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant L720E | Escherichia coli | L-glutamine | 0.84 | 0.18 | 4.7 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant L720E, L-glutamine |
| carbamoyl phosphate synthetasevariant M174E | Escherichia coli | adenosine 5'-diphosphate | 0.1 | 0.088 | 1.1 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant M174E, adenosine 5'-diphosphate |
| carbamoyl phosphate synthetasevariant M174E | Escherichia coli | adenosine 5'-triphosphateGln-dependent ATPase reaction (ADP formation) | 0.11 | 0.69 | 0.16 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant M174E, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant M174E | Escherichia coli | adenosine 5'-triphosphateKHCO3 | 0.053 | 0.36 | 0.15 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant M174E, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant M174E | Escherichia coli | L-glutamine | 0.15 | 0.39 | 0.38 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant M174E, L-glutamine |
| carbamoyl phosphate synthetasevariant M174E/M378E | Escherichia coli | adenosine 5'-diphosphate | 0.22 | 0.17 | 1.3 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant M174E/M378E, adenosine 5'-diphosphate |
| carbamoyl phosphate synthetasevariant M174E/M378E | Escherichia coli | adenosine 5'-triphosphateGln-dependent ATPase reaction (ADP formation) | 0.043 | 0.28 | 0.15 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant M174E/M378E, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant M174E/M378E | Escherichia coli | adenosine 5'-triphosphateKHCO3 | 0.028 | 0.29 | 0.097 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant M174E/M378E, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant M174E/M378E | Escherichia coli | L-glutamine | 0.1 | 0.47 | 0.21 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant M174E/M378E, L-glutamine |
| carbamoyl phosphate synthetasevariant M378E | Escherichia coli | adenosine 5'-diphosphate | 0.2 | 0.13 | 1.5 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant M378E, adenosine 5'-diphosphate |
| carbamoyl phosphate synthetasevariant M378E | Escherichia coli | adenosine 5'-triphosphateGln-dependent ATPase reaction (ADP formation) | 0.035 | 0.16 | 0.22 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant M378E, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant M378E | Escherichia coli | adenosine 5'-triphosphateKHCO3 | 0.013 | 0.03 | 0.43 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant M378E, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant M378E | Escherichia coli | L-glutamineglutaminase reaction (glutamate formation); varied glutamine | 0.13 | 0.35 | 0.37 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant M378E, L-glutamine |
| carbamoyl phosphate synthetasevariant M378E | Escherichia coli | L-glutamine | 0.012 | — | — | pH 7.6 | Open record: carbamoyl phosphate synthetase variant M378E, L-glutamine |
| carbamoyl phosphate synthetasevariant M911E | Escherichia coli | adenosine 5'-triphosphateGln-dependent ATPase reaction (ADP formation) | 0.5 | 1 | 0.50 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant M911E, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant M911E | Escherichia coli | adenosine 5'-triphosphateKHCO3 | 0.33 | 0.26 | 1.3 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant M911E, adenosine 5'-triphosphate |
| carbamoyl phosphate synthetasevariant M911E | Escherichia coli | L-glutamineglutaminase reaction (glutamate formation); varied glutamine | 0.14 | 0.18 | 0.78 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant M911E, L-glutamine |
| carbamoyl phosphate synthetasevariant M911E | Escherichia coli | L-glutamine | 0.015 | — | — | pH 7.6 | Open record: carbamoyl phosphate synthetase variant M911E, L-glutamine |