CatLogenzyme kinetics catalog

Values CatLog read from this paper

Access to the carbamate tunnel of carbamoyl phosphate synthetase

PMID 15081891doi:10.1016/j.abb.2004.02.031Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 52 records (70 values) from this paper: carbamoyl phosphate synthetase from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 18 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 6.3.5.5

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
carbamoyl phosphate synthetaseEscherichia coliadenosine 5'-diphosphate0.450.01628 calc.pH 7.6Open
carbamoyl phosphate synthetaseEscherichia coliadenosine 5'-triphosphate0.14——pH 7.6Open
carbamoyl phosphate synthetaseEscherichia coliammonium chloride3.91300.030 calc.pH 7.6Open
carbamoyl phosphate synthetaseEscherichia coliL-glutamineglutaminase partial reaction; 5.0 mM ATP2——pH 7.6Open
carbamoyl phosphate synthetaseEscherichia coliL-glutamineglutamine-dependent ATP hydrolysis; 5.0 mM ATP40.1233 calc.pH 7.6Open
carbamoyl phosphate synthetaseEscherichia coliL-glutamineoverall carbamoyl phosphate synthesis; 5 mM ATP1.4——pH 7.6Open
carbamoyl phosphate synthetasevariant E25Q/E383QEscherichia coliadenosine 5'-diphosphate0.0910.0312.9 calc.pH 7.6Open
carbamoyl phosphate synthetasevariant E25Q/E383QEscherichia coliadenosine 5'-triphosphate0.31——pH 7.6Open
carbamoyl phosphate synthetasevariant E25Q/E383QEscherichia coliammonium chloride0.51——pH 7.6Open
carbamoyl phosphate synthetasevariant E25Q/E383QEscherichia coliL-glutamineglutaminase partial reaction; 5.0 mM ATP0.96——pH 7.6Open
carbamoyl phosphate synthetasevariant E25Q/E383QEscherichia coliL-glutamineglutamine-dependent ATP hydrolysis; 5.0 mM ATP0.32——pH 7.6Open
carbamoyl phosphate synthetasevariant E25Q/E383QEscherichia coliL-glutamineoverall carbamoyl phosphate synthesis; 5 mM ATP0.03——pH 7.6Open
carbamoyl phosphate synthetasevariant E25Q/E383Q/E604QEscherichia coliadenosine 5'-diphosphate0.190.0228.6 calc.pH 7.6Open
carbamoyl phosphate synthetasevariant E25Q/E383Q/E604QEscherichia coliadenosine 5'-triphosphate0.061——pH 7.6Open
carbamoyl phosphate synthetasevariant E25Q/E383Q/E604QEscherichia coliammonium chloride0.097——pH 7.6Open
carbamoyl phosphate synthetasevariant E25Q/E383Q/E604QEscherichia coliL-glutamineglutaminase partial reaction0.42——pH 7.6Open
carbamoyl phosphate synthetasevariant E25Q/E383Q/E604QEscherichia coliL-glutamineglutamine-dependent ATP hydrolysis0.15——pH 7.6Open
carbamoyl phosphate synthetasevariant E383QEscherichia coliadenosine 5'-diphosphate0.280.0338.5 calc.pH 7.6Open
carbamoyl phosphate synthetasevariant E383QEscherichia coliadenosine 5'-triphosphate0.62——pH 7.6Open
carbamoyl phosphate synthetasevariant E383QEscherichia coliammonium chloride1.71100.015 calc.pH 7.6Open
carbamoyl phosphate synthetasevariant E383QEscherichia coliL-glutamineglutaminase partial reaction; 5.0 mM ATP0.84——pH 7.6Open
carbamoyl phosphate synthetasevariant E383QEscherichia coliL-glutamineglutamine-dependent ATP hydrolysis; 5.0 mM ATP3.20.1521 calc.pH 7.6Open
carbamoyl phosphate synthetasevariant E383QEscherichia coliL-glutamineoverall carbamoyl phosphate synthesis; 5 mM ATP1.3——pH 7.6Open
carbamoyl phosphate synthetasevariant E383Q/E916QEscherichia coliadenosine 5'-diphosphate0.390.113.5 calc.pH 7.6Open
carbamoyl phosphate synthetasevariant E383Q/E916QEscherichia coliadenosine 5'-triphosphate0.27——pH 7.6Open
carbamoyl phosphate synthetasevariant E383Q/E916QEscherichia coliL-glutamineglutaminase partial reaction; 5.0 mM ATP1.5——pH 7.6Open
carbamoyl phosphate synthetasevariant E383Q/E916QEscherichia coliL-glutamineglutamine-dependent ATP hydrolysis; 5.0 mM ATP40.1429 calc.pH 7.6Open
carbamoyl phosphate synthetasevariant E383Q/E916QEscherichia coliL-glutamineoverall carbamoyl phosphate synthesis; 5 mM ATP0.36——pH 7.6Open
carbamoyl phosphate synthetasevariant E577QEscherichia coliadenosine 5'-diphosphate0.0360.050.72 calc.pH 7.6Open
carbamoyl phosphate synthetasevariant E577QEscherichia coliadenosine 5'-triphosphate0.12——pH 7.6Open
carbamoyl phosphate synthetasevariant E577QEscherichia coliammonium chloride0.12——pH 7.6Open
carbamoyl phosphate synthetasevariant E577QEscherichia coliL-glutamineglutaminase partial reaction; 5.0 mM ATP0.091——pH 7.6Open
carbamoyl phosphate synthetasevariant E577QEscherichia coliL-glutamineglutamine-dependent ATP hydrolysis; 5.0 mM ATP0.17——pH 7.6Open
carbamoyl phosphate synthetasevariant E577QEscherichia coliL-glutamineoverall carbamoyl phosphate synthesis; 5 mM ATP0.0032——pH 7.6Open
carbamoyl phosphate synthetasevariant E604QEscherichia coliadenosine 5'-diphosphate0.210.0653.2 calc.pH 7.6Open
carbamoyl phosphate synthetasevariant E604QEscherichia coliadenosine 5'-triphosphate1.3——pH 7.6Open
carbamoyl phosphate synthetasevariant E604QEscherichia coliammonium chloride1.3——pH 7.6Open
carbamoyl phosphate synthetasevariant E604QEscherichia coliL-glutamineglutaminase partial reaction; 5.0 mM ATP0.81——pH 7.6Open
carbamoyl phosphate synthetasevariant E604QEscherichia coliL-glutamineglutamine-dependent ATP hydrolysis; 5.0 mM ATP1.5——pH 7.6Open
carbamoyl phosphate synthetasevariant E604QEscherichia coliL-glutamineoverall carbamoyl phosphate synthesis; 5 mM ATP0.38——pH 7.6Open
carbamoyl phosphate synthetasevariant E916QEscherichia coliadenosine 5'-diphosphate0.140.0178.2 calc.pH 7.6Open
carbamoyl phosphate synthetasevariant E916QEscherichia coliadenosine 5'-triphosphate0.6——pH 7.6Open
carbamoyl phosphate synthetasevariant E916QEscherichia coliammonium chloride1.31600.0081 calc.pH 7.6Open
carbamoyl phosphate synthetasevariant E916QEscherichia coliL-glutamineglutaminase partial reaction; 5.0 mM ATP1.5——pH 7.6Open
carbamoyl phosphate synthetasevariant E916QEscherichia coliL-glutamineglutamine-dependent ATP hydrolysis; 5.0 mM ATP4.20.1138 calc.pH 7.6Open
carbamoyl phosphate synthetasevariant E916QEscherichia coliL-glutamineoverall carbamoyl phosphate synthesis; 5 mM ATP0.21——pH 7.6Open
carbamoyl phosphate synthetasevariant R306AEscherichia coliadenosine 5'-triphosphatecosubstrate bicarbonate0.086——pH 7.6Open
carbamoyl phosphate synthetasevariant R306AEscherichia coliadenosine 5'-triphosphateglutamine-dependent ATP hydrolysis; 10 mM glutamine0.10.220.45 calc.pH 7.6Open
carbamoyl phosphate synthetasevariant R848AEscherichia coliadenosine 5'-diphosphate0.096.90.013 calc.pH 7.6Open
carbamoyl phosphate synthetasevariant R848AEscherichia coliadenosine 5'-triphosphatecosubstrate bicarbonate0.6——pH 7.6Open
carbamoyl phosphate synthetasevariant R848AEscherichia coliadenosine 5'-triphosphateglutamine-dependent ATP hydrolysis; 10 mM glutamine0.950.42.4 calc.pH 7.6Open
carbamoyl phosphate synthetasevariant R848AEscherichia coliL-glutamine0.006——pH 7.6Open