Values CatLog read from this paper
Perforation of the tunnel wall in carbamoyl phosphate synthetase derails the passage of ammonia between sequential active sites
PMID 15122899doi:10.1021/bi049945+Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 36 records (70 values) from this paper: carbamoyl phosphate synthetase from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 34 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| carbamoyl phosphate synthetase | Escherichia coli | ammoniumNH4+-dependent ATPase reaction (ADP formation); 0.2 mM NADH | 3.9 | 130 | 0.030 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase, ammonium |
| carbamoyl phosphate synthetase | Escherichia coli | ammonium | 1.9 | 130 | 0.015 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase, ammonium |
| carbamoyl phosphate synthetase | Escherichia coli | L-glutamineglutaminase reaction (glutamate formation) | 2 | 0.14 | 14 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase, L-glutamine |
| carbamoyl phosphate synthetase | Escherichia coli | L-glutamineGln-dependent ATPase reaction (ADP formation); 0.2 mM NADH | 3.8 | 0.1 | 38 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase, L-glutamine |
| carbamoyl phosphate synthetase | Escherichia coli | L-glutaminevaried glutamine | 1.4 | 0.11 | 13 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase, L-glutamine |
| carbamoyl phosphate synthetase | Escherichia coli | L-glutamine | 0.0016 | — | — | Open record: carbamoyl phosphate synthetase, L-glutamine | |
| carbamoyl phosphate synthetasevariant αD362A | Escherichia coli | ammoniumNH4+-dependent ATPase reaction (ADP formation); 0.2 mM NADH | 9.5 | 120 | 0.079 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant αD362A, ammonium |
| carbamoyl phosphate synthetasevariant αD362A | Escherichia coli | ammonium | 3.8 | 24 | 0.16 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant αD362A, ammonium |
| carbamoyl phosphate synthetasevariant αD362A | Escherichia coli | L-glutamineglutaminase reaction (glutamate formation) | 2.1 | 0.092 | 23 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant αD362A, L-glutamine |
| carbamoyl phosphate synthetasevariant αD362A | Escherichia coli | L-glutamineGln-dependent ATPase reaction (ADP formation); 0.2 mM NADH | 4.7 | 0.066 | 71 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant αD362A, L-glutamine |
| carbamoyl phosphate synthetasevariant αD362A | Escherichia coli | L-glutaminevaried glutamine | 2.4 | 0.19 | 13 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant αD362A, L-glutamine |
| carbamoyl phosphate synthetasevariant αD362A/βR265A | Escherichia coli | ammoniumNH4+-dependent ATPase reaction (ADP formation); 0.2 mM NADH | 10.3 | 55 | 0.19 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant αD362A/βR265A, ammonium |
| carbamoyl phosphate synthetasevariant αD362A/βR265A | Escherichia coli | ammonium | 5.8 | 59 | 0.098 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant αD362A/βR265A, ammonium |
| carbamoyl phosphate synthetasevariant αD362A/βR265A | Escherichia coli | L-glutamineglutaminase reaction (glutamate formation) | 3.2 | 0.38 | 8.4 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant αD362A/βR265A, L-glutamine |
| carbamoyl phosphate synthetasevariant αD362A/βR265A | Escherichia coli | L-glutamineGln-dependent ATPase reaction (ADP formation); 0.2 mM NADH | 2.3 | 5.5 | 0.42 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant αD362A/βR265A, L-glutamine |
| carbamoyl phosphate synthetasevariant αD362A/βR265A | Escherichia coli | L-glutaminevaried glutamine | 1.2 | 7.8 | 0.15 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant αD362A/βR265A, L-glutamine |
| carbamoyl phosphate synthetasevariant αP360A/αH361A | Escherichia coli | L-glutamineglutaminase reaction (glutamate formation) | 3.4 | 0.39 | 8.7 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant αP360A/αH361A, L-glutamine |
| carbamoyl phosphate synthetasevariant αP360A/αH361A | Escherichia coli | L-glutamineGln-dependent ATPase reaction (ADP formation); 0.2 mM NADH | 6 | 0.4 | 15 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant αP360A/αH361A, L-glutamine |
| carbamoyl phosphate synthetasevariant αP360A/αH361A | Escherichia coli | L-glutaminevaried glutamine | 2.3 | 0.36 | 6.4 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant αP360A/αH361A, L-glutamine |
| carbamoyl phosphate synthetasevariant αP360A/αH361A/βR265A | Escherichia coli | ammonia | — | — | 0.208 | pH 7.6 | Open record: carbamoyl phosphate synthetase variant αP360A/αH361A/βR265A, ammonia |
| carbamoyl phosphate synthetasevariant αP360A/αH361A/βR265A | Escherichia coli | ammoniumNH4+-dependent ATPase reaction (ADP formation); 0.2 mM NADH | 4.7 | 59 | 0.080 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant αP360A/αH361A/βR265A, ammonium |
| carbamoyl phosphate synthetasevariant αP360A/αH361A/βR265A | Escherichia coli | ammonium | 2.5 | 12 | 0.21 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant αP360A/αH361A/βR265A, ammonium |
| carbamoyl phosphate synthetasevariant βQ262A/βR265A | Escherichia coli | ammoniumNH4+-dependent ATPase reaction (ADP formation); 0.2 mM NADH | 5 | 58 | 0.086 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant βQ262A/βR265A, ammonium |
| carbamoyl phosphate synthetasevariant βQ262A/βR265A | Escherichia coli | ammonium | 3.6 | 26 | 0.14 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant βQ262A/βR265A, ammonium |
| carbamoyl phosphate synthetasevariant βQ262A/βR265A | Escherichia coli | L-glutamineglutaminase reaction (glutamate formation) | 2.6 | 6.4 | 0.41 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant βQ262A/βR265A, L-glutamine |
| carbamoyl phosphate synthetasevariant βQ262A/βR265A | Escherichia coli | L-glutamineGln-dependent ATPase reaction (ADP formation); 0.2 mM NADH | 2.9 | 4.1 | 0.71 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant βQ262A/βR265A, L-glutamine |
| carbamoyl phosphate synthetasevariant βQ262A/βR265A | Escherichia coli | L-glutaminevaried glutamine | 1 | 4.7 | 0.21 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant βQ262A/βR265A, L-glutamine |
| carbamoyl phosphate synthetasevariant βQ262A/βR265A/βN266A | Escherichia coli | ammonium | 6.7 | 56 | 0.12 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant βQ262A/βR265A/βN266A, ammonium |
| carbamoyl phosphate synthetasevariant βQ262A/βR265A/βN266A | Escherichia coli | L-glutamineglutaminase reaction (glutamate formation) | 1.7 | 5.5 | 0.31 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant βQ262A/βR265A/βN266A, L-glutamine |
| carbamoyl phosphate synthetasevariant βQ262A/βR265A/βN266A | Escherichia coli | L-glutamineGln-dependent ATPase reaction (ADP formation); 0.2 mM NADH | 1.9 | 1.9 | 1.0 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant βQ262A/βR265A/βN266A, L-glutamine |
| carbamoyl phosphate synthetasevariant βQ262A/βR265A/βN266A | Escherichia coli | L-glutaminevaried glutamine | 0.88 | 14.9 | 0.059 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant βQ262A/βR265A/βN266A, L-glutamine |
| carbamoyl phosphate synthetasevariant βR265A | Escherichia coli | ammoniumNH4+-dependent ATPase reaction (ADP formation); 0.2 mM NADH | 5.7 | 41 | 0.14 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant βR265A, ammonium |
| carbamoyl phosphate synthetasevariant βR265A | Escherichia coli | ammonium | 2.5 | 26 | 0.096 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant βR265A, ammonium |
| carbamoyl phosphate synthetasevariant βR265A | Escherichia coli | L-glutamineglutaminase reaction (glutamate formation) | 3.7 | 0.36 | 10 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant βR265A, L-glutamine |
| carbamoyl phosphate synthetasevariant βR265A | Escherichia coli | L-glutamineGln-dependent ATPase reaction (ADP formation); 0.2 mM NADH | 2.8 | 6.4 | 0.44 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant βR265A, L-glutamine |
| carbamoyl phosphate synthetasevariant βR265A | Escherichia coli | L-glutaminevaried glutamine | 1.1 | 7.6 | 0.14 calc. | pH 7.6 | Open record: carbamoyl phosphate synthetase variant βR265A, L-glutamine |