Values CatLog read from this paper
Kinetic characterization of yeast pyruvate carboxylase isozyme Pyc1 and the Pyc1 mutant, C249A
PMID 14744153doi:10.1021/bi035575yOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 11 records (15 values) from this paper: pyruvate carboxylase isozyme 1 (Pyc1) from Saccharomyces cerevisiae.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 4 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| pyruvate carboxylase isozyme 1 (Pyc1) | Saccharomyces cerevisiae | carbamoyl phosphatewith 0.25 mM acetyl-CoA and 5.1 mM K+ | 0.11 | 11.3 | 0.0097 calc. | 30 °C | Open record: pyruvate carboxylase isozyme 1 (Pyc1), carbamoyl phosphate |
| pyruvate carboxylase isozyme 1 (Pyc1) | Saccharomyces cerevisiae | carbamoyl phosphatewithout acetyl-CoA and K+ | 0.11 | 12.3 | 0.0089 calc. | 30 °C | Open record: pyruvate carboxylase isozyme 1 (Pyc1), carbamoyl phosphate |
| pyruvate carboxylase isozyme 1 (Pyc1) | Saccharomyces cerevisiae | pyruvatesaturating K+-free acetyl-CoA; no K+ | 59.5 | — | — | pH 7.8, 30 °C | Open record: pyruvate carboxylase isozyme 1 (Pyc1), pyruvate |
| pyruvate carboxylase isozyme 1 (Pyc1) | Saccharomyces cerevisiae | pyruvatesaturating K+; no acetyl-CoA | 29.9 | — | — | pH 7.8, 30 °C | Open record: pyruvate carboxylase isozyme 1 (Pyc1), pyruvate |
| pyruvate carboxylase isozyme 1 (Pyc1)variant C249A | Saccharomyces cerevisiae | bicarbonate | — | 2.3 | — | pH 7.8, 30 °C | Open record: pyruvate carboxylase isozyme 1 (Pyc1) variant C249A, bicarbonate |
| pyruvate carboxylase isozyme 1 (Pyc1)variant C249A | Saccharomyces cerevisiae | carbamoyl phosphatewith 1 mM acetyl-CoA and 20.5 mM K+ | 0.043 | 1.7 | 0.025 calc. | 30 °C | Open record: pyruvate carboxylase isozyme 1 (Pyc1) variant C249A, carbamoyl phosphate |
| pyruvate carboxylase isozyme 1 (Pyc1)variant C249A | Saccharomyces cerevisiae | carbamoyl phosphatewithout acetyl-CoA and K+ | 0.13 | 10.7 | 0.012 calc. | 30 °C | Open record: pyruvate carboxylase isozyme 1 (Pyc1) variant C249A, carbamoyl phosphate |
| pyruvate carboxylase isozyme 1 (Pyc1)variant C249A | Saccharomyces cerevisiae | Mg-ATP (adenosine 5'-triphosphate, magnesium complex) | — | 0.05 | — | pH 7.8, 30 °C | Open record: pyruvate carboxylase isozyme 1 (Pyc1) variant C249A, Mg-ATP (adenosine 5'-triphosphate, magnesium complex) |
| pyruvate carboxylase isozyme 1 (Pyc1)variant C249A | Saccharomyces cerevisiae | pyruvate1 mM acetyl-CoA and 20.5 mM K+ | — | 0.45 | — | pH 7.8, 30 °C | Open record: pyruvate carboxylase isozyme 1 (Pyc1) variant C249A, pyruvate |
| pyruvate carboxylase isozyme 1 (Pyc1)variant C249A | Saccharomyces cerevisiae | pyruvatefixed substrates 2.5 mM ATP; saturating K+-free acetyl-CoA | 20.1 | — | — | pH 7.8, 30 °C | Open record: pyruvate carboxylase isozyme 1 (Pyc1) variant C249A, pyruvate |
| pyruvate carboxylase isozyme 1 (Pyc1)variant C249A | Saccharomyces cerevisiae | pyruvatefixed substrates 2.5 mM ATP; saturating K+; no acetyl-CoA | 2.94 | — | — | pH 7.8, 30 °C | Open record: pyruvate carboxylase isozyme 1 (Pyc1) variant C249A, pyruvate |