EC 6.4.1.1: pyruvate carboxylase
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CatLog read 83 records for EC 6.4.1.1 from 3 papers, for 2 organisms. Names in these papers: pyruvate carboxylase, pyruvate carboxylase (RePC), pyruvate carboxylase isozyme 1 (Pyc1).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 109 records for EC 6.4.1.1 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 6.4.1.1 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Roles of Arg427 and Arg472 in the binding and allosteric effects of acetyl CoA in pyruvate carboxylase | Rhizobium etli | 40 |
| Activation and inhibition of pyruvate carboxylase from Rhizobium etli | Rhizobium etli | 32 |
| Kinetic characterization of yeast pyruvate carboxylase isozyme Pyc1 and the Pyc1 mutant, C249A | Saccharomyces cerevisiae | 11 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| pyruvate carboxylase | Rhizobium etli | biotin | 1.47 | — | — | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase, biotin |
| pyruvate carboxylase | Rhizobium etli | MgADPsaturating acetyl CoA (0.25 mM) | 0.27 | — | — | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase, MgADP |
| pyruvate carboxylase | Rhizobium etli | MgADPno acetyl CoA | 0.19 | — | — | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase, MgADP |
| pyruvate carboxylase | Rhizobium etli | MgATPpyruvate carboxylation; MgATP varied (0.02-1.5 mM); Table 3 | 10.9 | 0.11 | 99 | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase, MgATP |
| pyruvate carboxylase | Rhizobium etli | MgATPbicarbonate-dependent MgATP cleavage (no pyruvate); 1 mM ATP | 1.6 | — | — | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase, MgATP |
| pyruvate carboxylase | Rhizobium etli | MgATPbicarbonate-dependent MgATP cleavage (no pyruvate); 1 mM ATP | 0.19 | — | — | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase, MgATP |
| pyruvate carboxylase | Rhizobium etli | pyruvate | 10.6 | — | — | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase, pyruvate |
| pyruvate carboxylase | Rhizobium etli | pyruvateno acetyl CoA (acetyl CoA-independent reaction, k°cat) | 0.15 | — | — | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase, pyruvate |
| pyruvate carboxylasevariant R427K | Rhizobium etli | biotin | 0.081 | — | — | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase variant R427K, biotin |
| pyruvate carboxylasevariant R427K | Rhizobium etli | MgADPsaturating acetyl CoA (2 mM) | 0.056 | — | — | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase variant R427K, MgADP |
| pyruvate carboxylasevariant R427K | Rhizobium etli | MgADPno acetyl CoA | 0.077 | — | — | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase variant R427K, MgADP |
| pyruvate carboxylasevariant R427K | Rhizobium etli | MgATPpyruvate carboxylation; MgATP varied (0.02-1.5 mM); Table 3 | 3.4 | 0.29 | 12 | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase variant R427K, MgATP |
| pyruvate carboxylasevariant R427K | Rhizobium etli | MgATPbicarbonate-dependent MgATP cleavage (no pyruvate); 1 mM ATP | 0.0064 | — | — | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase variant R427K, MgATP |
| pyruvate carboxylasevariant R427K | Rhizobium etli | MgATPbicarbonate-dependent MgATP cleavage (no pyruvate); 1 mM ATP | 0.0016 | — | — | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase variant R427K, MgATP |
| pyruvate carboxylasevariant R427K | Rhizobium etli | pyruvate | 3.4 | — | — | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase variant R427K, pyruvate |
| pyruvate carboxylasevariant R427K | Rhizobium etli | pyruvateno acetyl CoA (acetyl CoA-independent reaction, k°cat) | 0.67 | — | — | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase variant R427K, pyruvate |
| pyruvate carboxylasevariant R427S | Rhizobium etli | biotin | 0.094 | — | — | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase variant R427S, biotin |
| pyruvate carboxylasevariant R427S | Rhizobium etli | MgADPsaturating acetyl CoA (0.5 mM) | 0.048 | — | — | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase variant R427S, MgADP |
| pyruvate carboxylasevariant R427S | Rhizobium etli | MgADPno acetyl CoA | 0.032 | — | — | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase variant R427S, MgADP |
| pyruvate carboxylasevariant R427S | Rhizobium etli | MgATPpyruvate carboxylation; MgATP varied (0.02-1.5 mM); Table 3 | 4.2 | 0.16 | 26 | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase variant R427S, MgATP |
| pyruvate carboxylasevariant R427S | Rhizobium etli | MgATPbicarbonate-dependent MgATP cleavage (no pyruvate); 1 mM ATP | 0.017 | — | — | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase variant R427S, MgATP |
| pyruvate carboxylasevariant R427S | Rhizobium etli | MgATPbicarbonate-dependent MgATP cleavage (no pyruvate); 1 mM ATP | 0.011 | — | — | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase variant R427S, MgATP |
| pyruvate carboxylasevariant R427S | Rhizobium etli | pyruvate | 4.3 | — | — | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase variant R427S, pyruvate |
| pyruvate carboxylasevariant R427S | Rhizobium etli | pyruvateno acetyl CoA (acetyl CoA-independent reaction, k°cat) | 0.26 | — | — | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase variant R427S, pyruvate |
| pyruvate carboxylasevariant R472K | Rhizobium etli | biotin | 0.046 | — | — | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase variant R472K, biotin |
| pyruvate carboxylasevariant R472K | Rhizobium etli | MgADPsaturating acetyl CoA (5 mM) | 0.021 | — | — | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase variant R472K, MgADP |
| pyruvate carboxylasevariant R472K | Rhizobium etli | MgADPno acetyl CoA | 0.039 | — | — | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase variant R472K, MgADP |
| pyruvate carboxylasevariant R472K | Rhizobium etli | MgATPpyruvate carboxylation; MgATP varied (0.02-1.5 mM); Table 3 | 1.9 | 0.7 | 3 | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase variant R472K, MgATP |
| pyruvate carboxylasevariant R472K | Rhizobium etli | MgATPbicarbonate-dependent MgATP cleavage (no pyruvate); 1 mM ATP | 0.009 | — | — | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase variant R472K, MgATP |
| pyruvate carboxylasevariant R472K | Rhizobium etli | MgATPbicarbonate-dependent MgATP cleavage (no pyruvate); 1 mM ATP | 0.0017 | — | — | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase variant R472K, MgATP |
| pyruvate carboxylasevariant R472K | Rhizobium etli | pyruvate | 3.4 | — | — | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase variant R472K, pyruvate |
| pyruvate carboxylasevariant R472K | Rhizobium etli | pyruvateno acetyl CoA (acetyl CoA-independent reaction, k°cat) | 0.17 | — | — | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase variant R472K, pyruvate |
| pyruvate carboxylasevariant R472S | Rhizobium etli | biotin | 0.034 | — | — | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase variant R472S, biotin |
| pyruvate carboxylasevariant R472S | Rhizobium etli | MgADPsaturating acetyl CoA (5 mM) | 0.015 | — | — | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase variant R472S, MgADP |
| pyruvate carboxylasevariant R472S | Rhizobium etli | MgADPno acetyl CoA | 0.026 | — | — | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase variant R472S, MgADP |
| pyruvate carboxylasevariant R472S | Rhizobium etli | MgATPpyruvate carboxylation; MgATP varied (0.02-1.5 mM); Table 3 | 2 | 1 | 2 | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase variant R472S, MgATP |
| pyruvate carboxylasevariant R472S | Rhizobium etli | MgATPbicarbonate-dependent MgATP cleavage (no pyruvate); 1 mM ATP | 0.009 | — | — | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase variant R472S, MgATP |
| pyruvate carboxylasevariant R472S | Rhizobium etli | MgATPbicarbonate-dependent MgATP cleavage (no pyruvate); 1 mM ATP | 0.0023 | — | — | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase variant R472S, MgATP |
| pyruvate carboxylasevariant R472S | Rhizobium etli | pyruvate | 2.6 | — | — | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase variant R472S, pyruvate |
| pyruvate carboxylasevariant R472S | Rhizobium etli | pyruvateno acetyl CoA (acetyl CoA-independent reaction, k°cat) | 0.1 | — | — | pH 7.8, 30 °C | PMID 22985389 | Open record: pyruvate carboxylase variant R472S, pyruvate |
| pyruvate carboxylase (RePC) | Rhizobium etli | acetyl phosphateMgADP phosphorylation (BC domain partial reverse reaction) | 0.0093 | 0.34 | 0.0283 | pH 7.5, 25 °C | PMID 21958066 | Open record: pyruvate carboxylase (RePC), acetyl phosphate |
| pyruvate carboxylase (RePC) | Rhizobium etli | acetyl phosphateMgADP phosphorylation; with 10 mM free biotin | 0.0048 | 0.14 | 0.035 | pH 7.5, 25 °C | PMID 21958066 | Open record: pyruvate carboxylase (RePC), acetyl phosphate |
| pyruvate carboxylase (RePC) | Rhizobium etli | bicarbonate | 11.7 | 10.8 | 1.08 | pH 7.5, 25 °C | PMID 21958066 | Open record: pyruvate carboxylase (RePC), bicarbonate |
| pyruvate carboxylase (RePC) | Rhizobium etli | carbamoyl phosphateMgADP phosphorylation (BC domain partial reverse reaction) | 0.0402 | 2.6 | 0.0155 | pH 7.5, 25 °C | PMID 21958066 | Open record: pyruvate carboxylase (RePC), carbamoyl phosphate |
| pyruvate carboxylase (RePC) | Rhizobium etli | carbamoyl phosphateMgADP phosphorylation by carbamoyl phosphate; no acetyl-CoA | 0.0405 | — | — | pH 7.5, 25 °C | PMID 21958066 | Open record: pyruvate carboxylase (RePC), carbamoyl phosphate |
| pyruvate carboxylase (RePC) | Rhizobium etli | carbamoyl phosphateMgADP phosphorylation; carbamoyl phosphate varied (1-20 mM) | 0.068 | 3.3 | 0.02 | pH 7.5, 25 °C | PMID 21958066 | Open record: pyruvate carboxylase (RePC), carbamoyl phosphate |
| pyruvate carboxylase (RePC) | Rhizobium etli | carbamoyl phosphateMgADP phosphorylation; 0.25 mM acetyl-CoA | 0.0542 | 5.2 | 0.010 calc. | pH 7.5, 25 °C | PMID 21958066 | Open record: pyruvate carboxylase (RePC), carbamoyl phosphate |
| pyruvate carboxylase (RePC) | Rhizobium etli | MgADP | 0.01392 | 0.12 | 0.1158 | pH 7.5, 25 °C | PMID 21958066 | Open record: pyruvate carboxylase (RePC), MgADP |
| pyruvate carboxylase (RePC) | Rhizobium etli | MgATPpyruvate carboxylation (full forward); 12 mM pyruvate | 6.6 | 0.145 | 44.5 | pH 7.5, 25 °C | PMID 21958066 | Open record: pyruvate carboxylase (RePC), MgATP |
| pyruvate carboxylase (RePC) | Rhizobium etli | MgATPHCO3−-dependent ATPase (no pyruvate); 20 mM HCO3− | 0.043 | 0.022 | 1.92 | pH 7.5, 25 °C | PMID 21958066 | Open record: pyruvate carboxylase (RePC), MgATP |
| pyruvate carboxylase (RePC) | Rhizobium etli | MgATPHCO3−-dependent ATPase (no pyruvate); no acetyl-CoA | 0.00912 | — | — | pH 7.5, 25 °C | PMID 21958066 | Open record: pyruvate carboxylase (RePC), MgATP |
| pyruvate carboxylase (RePC) | Rhizobium etli | MgATPHCO3−-dependent ATPase (no pyruvate); 15 mM HCO3− | 0.0503 | 0.018 | 2.78 | pH 7.5, 25 °C | PMID 21958066 | Open record: pyruvate carboxylase (RePC), MgATP |
| pyruvate carboxylase (RePC) | Rhizobium etli | MgATPpyruvate carboxylation; 25 mM HCO3−; 12 mM pyruvate | 10.33 | 0.36 | 29 calc. | pH 7.5, 25 °C | PMID 21958066 | Open record: pyruvate carboxylase (RePC), MgATP |
| pyruvate carboxylase (RePC) | Rhizobium etli | oxaloacetate0.95 mM oxaloacetate; 1 mM oxamate; 0.25 mM acetyl-CoA | 0.1505 | — | — | pH 7.5, 25 °C | PMID 21958066 | Open record: pyruvate carboxylase (RePC), oxaloacetate |
| pyruvate carboxylase (RePC) | Rhizobium etli | oxaloacetateno acetyl-CoA; all substrates saturating; 7.0 mM MgCl2 | 0.00253 | — | — | pH 7.5, 25 °C | PMID 21958066 | Open record: pyruvate carboxylase (RePC), oxaloacetate |
| pyruvate carboxylase (RePC) | Rhizobium etli | oxaloacetateoxamate-induced decarboxylation of oxaloacetate | 0.1 | — | — | pH 7.5, 25 °C | PMID 21958066 | Open record: pyruvate carboxylase (RePC), oxaloacetate |
| pyruvate carboxylase (RePC) | Rhizobium etli | pyruvatepyruvate carboxylation (full forward); 25 mM HCO3−; Table 1 | 7.33 | 0.15 | 48.3 | pH 7.5, 25 °C | PMID 21958066 | Open record: pyruvate carboxylase (RePC), pyruvate |
| pyruvate carboxylase (RePC) | Rhizobium etli | pyruvatepyruvate carboxylation (full forward); no acetyl-CoA | 1.097 | — | — | pH 7.5, 25 °C | PMID 21958066 | Open record: pyruvate carboxylase (RePC), pyruvate |
| pyruvate carboxylase (RePC) | Rhizobium etli | pyruvatepyruvate carboxylation (oxaloacetate formation); 15 mM HCO3− | 5.17 | 0.65 | 7.83 | pH 7.5, 25 °C | PMID 21958066 | Open record: pyruvate carboxylase (RePC), pyruvate |
| pyruvate carboxylase (RePC) | Rhizobium etli | pyruvatepyruvate carboxylation (oxaloacetate formation); 15 mM HCO3− | 0.0253 | 0.013 | 1.92 | pH 7.5, 25 °C | PMID 21958066 | Open record: pyruvate carboxylase (RePC), pyruvate |
| pyruvate carboxylase (RePC)variant K1119Q | Rhizobium etli | acetyl phosphateno free biotin | 0.0078 | 0.29 | 0.027 | pH 7.5, 25 °C | PMID 21958066 | Open record: pyruvate carboxylase (RePC) variant K1119Q, acetyl phosphate |
| pyruvate carboxylase (RePC)variant K1119Q | Rhizobium etli | acetyl phosphatewith 10 mM free biotin | 0.0073 | 0.37 | 0.0198 | pH 7.5, 25 °C | PMID 21958066 | Open record: pyruvate carboxylase (RePC) variant K1119Q, acetyl phosphate |
| pyruvate carboxylase (RePC)variant K1119Q | Rhizobium etli | carbamoyl phosphateno free biotin | 2.3 × 10⁻⁴ | 0.88 | 0.0027 | pH 7.5, 25 °C | PMID 21958066 | Open record: pyruvate carboxylase (RePC) variant K1119Q, carbamoyl phosphate |
| pyruvate carboxylase (RePC)variant K1119Q | Rhizobium etli | carbamoyl phosphatewith 10 mM free biotin | 0.035 | 3.9 | 0.009 | pH 7.5, 25 °C | PMID 21958066 | Open record: pyruvate carboxylase (RePC) variant K1119Q, carbamoyl phosphate |
| pyruvate carboxylase (RePC)variant K1119Q | Rhizobium etli | MgATPno free biotin | 0.00137 | 1.2 | 0.00113 | pH 7.5, 25 °C | PMID 21958066 | Open record: pyruvate carboxylase (RePC) variant K1119Q, MgATP |
| pyruvate carboxylase (RePC)variant K1119Q | Rhizobium etli | MgATPwith 10 mM free biotin | 0.0448 | 0.04 | 1.125 | pH 7.5, 25 °C | PMID 21958066 | Open record: pyruvate carboxylase (RePC) variant K1119Q, MgATP |
| pyruvate carboxylase (RePC)variant T882A | Rhizobium etli | acetyl phosphateno free biotin | 0.005 | 0.22 | 0.0227 | pH 7.5, 25 °C | PMID 21958066 | Open record: pyruvate carboxylase (RePC) variant T882A, acetyl phosphate |
| pyruvate carboxylase (RePC)variant T882A | Rhizobium etli | acetyl phosphatewith 10 mM free biotin | 0.002 | 0.14 | 0.0142 | pH 7.5, 25 °C | PMID 21958066 | Open record: pyruvate carboxylase (RePC) variant T882A, acetyl phosphate |
| pyruvate carboxylase (RePC)variant T882A | Rhizobium etli | carbamoyl phosphateno free biotin | 0.4 | 3.4 | 0.117 | pH 7.5, 25 °C | PMID 21958066 | Open record: pyruvate carboxylase (RePC) variant T882A, carbamoyl phosphate |
| pyruvate carboxylase (RePC)variant T882A | Rhizobium etli | carbamoyl phosphatewith 10 mM free biotin | 0.535 | 2.9 | 0.18 | pH 7.5, 25 °C | PMID 21958066 | Open record: pyruvate carboxylase (RePC) variant T882A, carbamoyl phosphate |
| pyruvate carboxylase (RePC)variant T882A | Rhizobium etli | MgATPno free biotin | 0.23 | 0.054 | 4.33 | pH 7.5, 25 °C | PMID 21958066 | Open record: pyruvate carboxylase (RePC) variant T882A, MgATP |
| pyruvate carboxylase (RePC)variant T882A | Rhizobium etli | MgATPwith 10 mM free biotin | 0.63 | 0.09 | 7.2 | pH 7.5, 25 °C | PMID 21958066 | Open record: pyruvate carboxylase (RePC) variant T882A, MgATP |
| pyruvate carboxylase isozyme 1 (Pyc1) | Saccharomyces cerevisiae | carbamoyl phosphatewith 0.25 mM acetyl-CoA and 5.1 mM K+ | 0.11 | 11.3 | 0.0097 calc. | 30 °C | PMID 14744153 | Open record: pyruvate carboxylase isozyme 1 (Pyc1), carbamoyl phosphate |
| pyruvate carboxylase isozyme 1 (Pyc1) | Saccharomyces cerevisiae | carbamoyl phosphatewithout acetyl-CoA and K+ | 0.11 | 12.3 | 0.0089 calc. | 30 °C | PMID 14744153 | Open record: pyruvate carboxylase isozyme 1 (Pyc1), carbamoyl phosphate |
| pyruvate carboxylase isozyme 1 (Pyc1) | Saccharomyces cerevisiae | pyruvatesaturating K+-free acetyl-CoA; no K+ | 59.5 | — | — | pH 7.8, 30 °C | PMID 14744153 | Open record: pyruvate carboxylase isozyme 1 (Pyc1), pyruvate |
| pyruvate carboxylase isozyme 1 (Pyc1) | Saccharomyces cerevisiae | pyruvatesaturating K+; no acetyl-CoA | 29.9 | — | — | pH 7.8, 30 °C | PMID 14744153 | Open record: pyruvate carboxylase isozyme 1 (Pyc1), pyruvate |
| pyruvate carboxylase isozyme 1 (Pyc1)variant C249A | Saccharomyces cerevisiae | bicarbonate | — | 2.3 | — | pH 7.8, 30 °C | PMID 14744153 | Open record: pyruvate carboxylase isozyme 1 (Pyc1) variant C249A, bicarbonate |
| pyruvate carboxylase isozyme 1 (Pyc1)variant C249A | Saccharomyces cerevisiae | carbamoyl phosphatewith 1 mM acetyl-CoA and 20.5 mM K+ | 0.043 | 1.7 | 0.025 calc. | 30 °C | PMID 14744153 | Open record: pyruvate carboxylase isozyme 1 (Pyc1) variant C249A, carbamoyl phosphate |
| pyruvate carboxylase isozyme 1 (Pyc1)variant C249A | Saccharomyces cerevisiae | carbamoyl phosphatewithout acetyl-CoA and K+ | 0.13 | 10.7 | 0.012 calc. | 30 °C | PMID 14744153 | Open record: pyruvate carboxylase isozyme 1 (Pyc1) variant C249A, carbamoyl phosphate |
| pyruvate carboxylase isozyme 1 (Pyc1)variant C249A | Saccharomyces cerevisiae | Mg-ATP (adenosine 5'-triphosphate, magnesium complex) | — | 0.05 | — | pH 7.8, 30 °C | PMID 14744153 | Open record: pyruvate carboxylase isozyme 1 (Pyc1) variant C249A, Mg-ATP (adenosine 5'-triphosphate, magnesium complex) |
| pyruvate carboxylase isozyme 1 (Pyc1)variant C249A | Saccharomyces cerevisiae | pyruvate1 mM acetyl-CoA and 20.5 mM K+ | — | 0.45 | — | pH 7.8, 30 °C | PMID 14744153 | Open record: pyruvate carboxylase isozyme 1 (Pyc1) variant C249A, pyruvate |
| pyruvate carboxylase isozyme 1 (Pyc1)variant C249A | Saccharomyces cerevisiae | pyruvatefixed substrates 2.5 mM ATP; saturating K+-free acetyl-CoA | 20.1 | — | — | pH 7.8, 30 °C | PMID 14744153 | Open record: pyruvate carboxylase isozyme 1 (Pyc1) variant C249A, pyruvate |
| pyruvate carboxylase isozyme 1 (Pyc1)variant C249A | Saccharomyces cerevisiae | pyruvatefixed substrates 2.5 mM ATP; saturating K+; no acetyl-CoA | 2.94 | — | — | pH 7.8, 30 °C | PMID 14744153 | Open record: pyruvate carboxylase isozyme 1 (Pyc1) variant C249A, pyruvate |