CatLogenzyme kinetics catalog

Values CatLog read from this paper

Roles of Arg427 and Arg472 in the binding and allosteric effects of acetyl CoA in pyruvate carboxylase

PMID 22985389doi:10.1021/bi301060dOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 40 records (50 values) from this paper: pyruvate carboxylase from Rhizobium etli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 6.4.1.1

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
pyruvate carboxylaseRhizobium etlibiotin1.47——pH 7.8, 30 °COpen
pyruvate carboxylaseRhizobium etliMgADPsaturating acetyl CoA (0.25 mM)0.27——pH 7.8, 30 °COpen
pyruvate carboxylaseRhizobium etliMgADPno acetyl CoA0.19——pH 7.8, 30 °COpen
pyruvate carboxylaseRhizobium etliMgATPpyruvate carboxylation; MgATP varied (0.02-1.5 mM); Table 310.90.1199pH 7.8, 30 °COpen
pyruvate carboxylaseRhizobium etliMgATPbicarbonate-dependent MgATP cleavage (no pyruvate); 1 mM ATP1.6——pH 7.8, 30 °COpen
pyruvate carboxylaseRhizobium etliMgATPbicarbonate-dependent MgATP cleavage (no pyruvate); 1 mM ATP0.19——pH 7.8, 30 °COpen
pyruvate carboxylaseRhizobium etlipyruvate10.6——pH 7.8, 30 °COpen
pyruvate carboxylaseRhizobium etlipyruvateno acetyl CoA (acetyl CoA-independent reaction, k°cat)0.15——pH 7.8, 30 °COpen
pyruvate carboxylasevariant R427KRhizobium etlibiotin0.081——pH 7.8, 30 °COpen
pyruvate carboxylasevariant R427KRhizobium etliMgADPsaturating acetyl CoA (2 mM)0.056——pH 7.8, 30 °COpen
pyruvate carboxylasevariant R427KRhizobium etliMgADPno acetyl CoA0.077——pH 7.8, 30 °COpen
pyruvate carboxylasevariant R427KRhizobium etliMgATPpyruvate carboxylation; MgATP varied (0.02-1.5 mM); Table 33.40.2912pH 7.8, 30 °COpen
pyruvate carboxylasevariant R427KRhizobium etliMgATPbicarbonate-dependent MgATP cleavage (no pyruvate); 1 mM ATP0.0064——pH 7.8, 30 °COpen
pyruvate carboxylasevariant R427KRhizobium etliMgATPbicarbonate-dependent MgATP cleavage (no pyruvate); 1 mM ATP0.0016——pH 7.8, 30 °COpen
pyruvate carboxylasevariant R427KRhizobium etlipyruvate3.4——pH 7.8, 30 °COpen
pyruvate carboxylasevariant R427KRhizobium etlipyruvateno acetyl CoA (acetyl CoA-independent reaction, k°cat)0.67——pH 7.8, 30 °COpen
pyruvate carboxylasevariant R427SRhizobium etlibiotin0.094——pH 7.8, 30 °COpen
pyruvate carboxylasevariant R427SRhizobium etliMgADPsaturating acetyl CoA (0.5 mM)0.048——pH 7.8, 30 °COpen
pyruvate carboxylasevariant R427SRhizobium etliMgADPno acetyl CoA0.032——pH 7.8, 30 °COpen
pyruvate carboxylasevariant R427SRhizobium etliMgATPpyruvate carboxylation; MgATP varied (0.02-1.5 mM); Table 34.20.1626pH 7.8, 30 °COpen
pyruvate carboxylasevariant R427SRhizobium etliMgATPbicarbonate-dependent MgATP cleavage (no pyruvate); 1 mM ATP0.017——pH 7.8, 30 °COpen
pyruvate carboxylasevariant R427SRhizobium etliMgATPbicarbonate-dependent MgATP cleavage (no pyruvate); 1 mM ATP0.011——pH 7.8, 30 °COpen
pyruvate carboxylasevariant R427SRhizobium etlipyruvate4.3——pH 7.8, 30 °COpen
pyruvate carboxylasevariant R427SRhizobium etlipyruvateno acetyl CoA (acetyl CoA-independent reaction, k°cat)0.26——pH 7.8, 30 °COpen
pyruvate carboxylasevariant R472KRhizobium etlibiotin0.046——pH 7.8, 30 °COpen
pyruvate carboxylasevariant R472KRhizobium etliMgADPsaturating acetyl CoA (5 mM)0.021——pH 7.8, 30 °COpen
pyruvate carboxylasevariant R472KRhizobium etliMgADPno acetyl CoA0.039——pH 7.8, 30 °COpen
pyruvate carboxylasevariant R472KRhizobium etliMgATPpyruvate carboxylation; MgATP varied (0.02-1.5 mM); Table 31.90.73pH 7.8, 30 °COpen
pyruvate carboxylasevariant R472KRhizobium etliMgATPbicarbonate-dependent MgATP cleavage (no pyruvate); 1 mM ATP0.009——pH 7.8, 30 °COpen
pyruvate carboxylasevariant R472KRhizobium etliMgATPbicarbonate-dependent MgATP cleavage (no pyruvate); 1 mM ATP0.0017——pH 7.8, 30 °COpen
pyruvate carboxylasevariant R472KRhizobium etlipyruvate3.4——pH 7.8, 30 °COpen
pyruvate carboxylasevariant R472KRhizobium etlipyruvateno acetyl CoA (acetyl CoA-independent reaction, k°cat)0.17——pH 7.8, 30 °COpen
pyruvate carboxylasevariant R472SRhizobium etlibiotin0.034——pH 7.8, 30 °COpen
pyruvate carboxylasevariant R472SRhizobium etliMgADPsaturating acetyl CoA (5 mM)0.015——pH 7.8, 30 °COpen
pyruvate carboxylasevariant R472SRhizobium etliMgADPno acetyl CoA0.026——pH 7.8, 30 °COpen
pyruvate carboxylasevariant R472SRhizobium etliMgATPpyruvate carboxylation; MgATP varied (0.02-1.5 mM); Table 3212pH 7.8, 30 °COpen
pyruvate carboxylasevariant R472SRhizobium etliMgATPbicarbonate-dependent MgATP cleavage (no pyruvate); 1 mM ATP0.009——pH 7.8, 30 °COpen
pyruvate carboxylasevariant R472SRhizobium etliMgATPbicarbonate-dependent MgATP cleavage (no pyruvate); 1 mM ATP0.0023——pH 7.8, 30 °COpen
pyruvate carboxylasevariant R472SRhizobium etlipyruvate2.6——pH 7.8, 30 °COpen
pyruvate carboxylasevariant R472SRhizobium etlipyruvateno acetyl CoA (acetyl CoA-independent reaction, k°cat)0.1——pH 7.8, 30 °COpen