Values CatLog read from this paper
Activation and inhibition of pyruvate carboxylase from Rhizobium etli
PMID 21958066doi:10.1021/bi201276rOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 32 records (82 values) from this paper: pyruvate carboxylase (RePC) from Rhizobium etli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| pyruvate carboxylase (RePC) | Rhizobium etli | acetyl phosphateMgADP phosphorylation (BC domain partial reverse reaction) | 0.0093 | 0.34 | 0.0283 | pH 7.5, 25 °C | Open record: pyruvate carboxylase (RePC), acetyl phosphate |
| pyruvate carboxylase (RePC) | Rhizobium etli | acetyl phosphateMgADP phosphorylation; with 10 mM free biotin | 0.0048 | 0.14 | 0.035 | pH 7.5, 25 °C | Open record: pyruvate carboxylase (RePC), acetyl phosphate |
| pyruvate carboxylase (RePC) | Rhizobium etli | bicarbonate | 11.7 | 10.8 | 1.08 | pH 7.5, 25 °C | Open record: pyruvate carboxylase (RePC), bicarbonate |
| pyruvate carboxylase (RePC) | Rhizobium etli | carbamoyl phosphateMgADP phosphorylation (BC domain partial reverse reaction) | 0.0402 | 2.6 | 0.0155 | pH 7.5, 25 °C | Open record: pyruvate carboxylase (RePC), carbamoyl phosphate |
| pyruvate carboxylase (RePC) | Rhizobium etli | carbamoyl phosphateMgADP phosphorylation by carbamoyl phosphate; no acetyl-CoA | 0.0405 | — | — | pH 7.5, 25 °C | Open record: pyruvate carboxylase (RePC), carbamoyl phosphate |
| pyruvate carboxylase (RePC) | Rhizobium etli | carbamoyl phosphateMgADP phosphorylation; carbamoyl phosphate varied (1-20 mM) | 0.068 | 3.3 | 0.02 | pH 7.5, 25 °C | Open record: pyruvate carboxylase (RePC), carbamoyl phosphate |
| pyruvate carboxylase (RePC) | Rhizobium etli | carbamoyl phosphateMgADP phosphorylation; 0.25 mM acetyl-CoA | 0.0542 | 5.2 | 0.010 calc. | pH 7.5, 25 °C | Open record: pyruvate carboxylase (RePC), carbamoyl phosphate |
| pyruvate carboxylase (RePC) | Rhizobium etli | MgADP | 0.01392 | 0.12 | 0.1158 | pH 7.5, 25 °C | Open record: pyruvate carboxylase (RePC), MgADP |
| pyruvate carboxylase (RePC) | Rhizobium etli | MgATPpyruvate carboxylation (full forward); 12 mM pyruvate | 6.6 | 0.145 | 44.5 | pH 7.5, 25 °C | Open record: pyruvate carboxylase (RePC), MgATP |
| pyruvate carboxylase (RePC) | Rhizobium etli | MgATPHCO3−-dependent ATPase (no pyruvate); 20 mM HCO3− | 0.043 | 0.022 | 1.92 | pH 7.5, 25 °C | Open record: pyruvate carboxylase (RePC), MgATP |
| pyruvate carboxylase (RePC) | Rhizobium etli | MgATPHCO3−-dependent ATPase (no pyruvate); no acetyl-CoA | 0.00912 | — | — | pH 7.5, 25 °C | Open record: pyruvate carboxylase (RePC), MgATP |
| pyruvate carboxylase (RePC) | Rhizobium etli | MgATPHCO3−-dependent ATPase (no pyruvate); 15 mM HCO3− | 0.0503 | 0.018 | 2.78 | pH 7.5, 25 °C | Open record: pyruvate carboxylase (RePC), MgATP |
| pyruvate carboxylase (RePC) | Rhizobium etli | MgATPpyruvate carboxylation; 25 mM HCO3−; 12 mM pyruvate | 10.33 | 0.36 | 29 calc. | pH 7.5, 25 °C | Open record: pyruvate carboxylase (RePC), MgATP |
| pyruvate carboxylase (RePC) | Rhizobium etli | oxaloacetate0.95 mM oxaloacetate; 1 mM oxamate; 0.25 mM acetyl-CoA | 0.1505 | — | — | pH 7.5, 25 °C | Open record: pyruvate carboxylase (RePC), oxaloacetate |
| pyruvate carboxylase (RePC) | Rhizobium etli | oxaloacetateno acetyl-CoA; all substrates saturating; 7.0 mM MgCl2 | 0.00253 | — | — | pH 7.5, 25 °C | Open record: pyruvate carboxylase (RePC), oxaloacetate |
| pyruvate carboxylase (RePC) | Rhizobium etli | oxaloacetateoxamate-induced decarboxylation of oxaloacetate | 0.1 | — | — | pH 7.5, 25 °C | Open record: pyruvate carboxylase (RePC), oxaloacetate |
| pyruvate carboxylase (RePC) | Rhizobium etli | pyruvatepyruvate carboxylation (full forward); 25 mM HCO3−; Table 1 | 7.33 | 0.15 | 48.3 | pH 7.5, 25 °C | Open record: pyruvate carboxylase (RePC), pyruvate |
| pyruvate carboxylase (RePC) | Rhizobium etli | pyruvatepyruvate carboxylation (full forward); no acetyl-CoA | 1.097 | — | — | pH 7.5, 25 °C | Open record: pyruvate carboxylase (RePC), pyruvate |
| pyruvate carboxylase (RePC) | Rhizobium etli | pyruvatepyruvate carboxylation (oxaloacetate formation); 15 mM HCO3− | 5.17 | 0.65 | 7.83 | pH 7.5, 25 °C | Open record: pyruvate carboxylase (RePC), pyruvate |
| pyruvate carboxylase (RePC) | Rhizobium etli | pyruvatepyruvate carboxylation (oxaloacetate formation); 15 mM HCO3− | 0.0253 | 0.013 | 1.92 | pH 7.5, 25 °C | Open record: pyruvate carboxylase (RePC), pyruvate |
| pyruvate carboxylase (RePC)variant K1119Q | Rhizobium etli | acetyl phosphateno free biotin | 0.0078 | 0.29 | 0.027 | pH 7.5, 25 °C | Open record: pyruvate carboxylase (RePC) variant K1119Q, acetyl phosphate |
| pyruvate carboxylase (RePC)variant K1119Q | Rhizobium etli | acetyl phosphatewith 10 mM free biotin | 0.0073 | 0.37 | 0.0198 | pH 7.5, 25 °C | Open record: pyruvate carboxylase (RePC) variant K1119Q, acetyl phosphate |
| pyruvate carboxylase (RePC)variant K1119Q | Rhizobium etli | carbamoyl phosphateno free biotin | 2.3 × 10⁻⁴ | 0.88 | 0.0027 | pH 7.5, 25 °C | Open record: pyruvate carboxylase (RePC) variant K1119Q, carbamoyl phosphate |
| pyruvate carboxylase (RePC)variant K1119Q | Rhizobium etli | carbamoyl phosphatewith 10 mM free biotin | 0.035 | 3.9 | 0.009 | pH 7.5, 25 °C | Open record: pyruvate carboxylase (RePC) variant K1119Q, carbamoyl phosphate |
| pyruvate carboxylase (RePC)variant K1119Q | Rhizobium etli | MgATPno free biotin | 0.00137 | 1.2 | 0.00113 | pH 7.5, 25 °C | Open record: pyruvate carboxylase (RePC) variant K1119Q, MgATP |
| pyruvate carboxylase (RePC)variant K1119Q | Rhizobium etli | MgATPwith 10 mM free biotin | 0.0448 | 0.04 | 1.125 | pH 7.5, 25 °C | Open record: pyruvate carboxylase (RePC) variant K1119Q, MgATP |
| pyruvate carboxylase (RePC)variant T882A | Rhizobium etli | acetyl phosphateno free biotin | 0.005 | 0.22 | 0.0227 | pH 7.5, 25 °C | Open record: pyruvate carboxylase (RePC) variant T882A, acetyl phosphate |
| pyruvate carboxylase (RePC)variant T882A | Rhizobium etli | acetyl phosphatewith 10 mM free biotin | 0.002 | 0.14 | 0.0142 | pH 7.5, 25 °C | Open record: pyruvate carboxylase (RePC) variant T882A, acetyl phosphate |
| pyruvate carboxylase (RePC)variant T882A | Rhizobium etli | carbamoyl phosphateno free biotin | 0.4 | 3.4 | 0.117 | pH 7.5, 25 °C | Open record: pyruvate carboxylase (RePC) variant T882A, carbamoyl phosphate |
| pyruvate carboxylase (RePC)variant T882A | Rhizobium etli | carbamoyl phosphatewith 10 mM free biotin | 0.535 | 2.9 | 0.18 | pH 7.5, 25 °C | Open record: pyruvate carboxylase (RePC) variant T882A, carbamoyl phosphate |
| pyruvate carboxylase (RePC)variant T882A | Rhizobium etli | MgATPno free biotin | 0.23 | 0.054 | 4.33 | pH 7.5, 25 °C | Open record: pyruvate carboxylase (RePC) variant T882A, MgATP |
| pyruvate carboxylase (RePC)variant T882A | Rhizobium etli | MgATPwith 10 mM free biotin | 0.63 | 0.09 | 7.2 | pH 7.5, 25 °C | Open record: pyruvate carboxylase (RePC) variant T882A, MgATP |