Values CatLog read from this paper
Conserved and nonconserved residues in the substrate binding site of 7,8-diaminopelargonic acid synthase from Escherichia coli are essential for catalysis
PMID 14756557doi:10.1021/bi0358059Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 18 records (31 values) from this paper: 7,8-diaminopelargonic acid synthase (DAPA synthase) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 13 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 7,8-diaminopelargonic acid synthase (DAPA synthase) | Escherichia coli | 7,8-diaminopelargonic acid | 0.79 | 0.001 | 790 calc. | pH 9 | Open record: 7,8-diaminopelargonic acid synthase (DAPA synthase), 7,8-diaminopelargonic acid |
| 7,8-diaminopelargonic acid synthase (DAPA synthase) | Escherichia coli | S-adenosyl-(5')-3-methylthiopropylamine | 0.016 | 0.75 | 0.021 calc. | pH 9 | Open record: 7,8-diaminopelargonic acid synthase (DAPA synthase), S-adenosyl-(5')-3-methylthiopropylamine |
| 7,8-diaminopelargonic acid synthase (DAPA synthase) | Escherichia coli | S-adenosyl-L-methionineapparent Km | 0.016 | 0.3 | 0.053 calc. | pH 9 | Open record: 7,8-diaminopelargonic acid synthase (DAPA synthase), S-adenosyl-L-methionine |
| 7,8-diaminopelargonic acid synthase (DAPA synthase) | Escherichia coli | S-adenosyl-L-methionine | — | — | 0.053 | pH 9 | Open record: 7,8-diaminopelargonic acid synthase (DAPA synthase), S-adenosyl-L-methionine |
| 7,8-diaminopelargonic acid synthase (DAPA synthase)variant R253A | Escherichia coli | 7,8-diaminopelargonic acid | 1.3 | 0.0022 | 590 calc. | pH 9 | Open record: 7,8-diaminopelargonic acid synthase (DAPA synthase) variant R253A, 7,8-diaminopelargonic acid |
| 7,8-diaminopelargonic acid synthase (DAPA synthase)variant R253K | Escherichia coli | 7,8-diaminopelargonic acid | 1.3 | 8 × 10⁻⁴ | 1.6 × 10³ calc. | pH 9 | Open record: 7,8-diaminopelargonic acid synthase (DAPA synthase) variant R253K, 7,8-diaminopelargonic acid |
| 7,8-diaminopelargonic acid synthase (DAPA synthase)variant R253K | Escherichia coli | S-adenosyl-(5')-3-methylthiopropylamine | 0.016 | 0.1 | 0.16 calc. | pH 9 | Open record: 7,8-diaminopelargonic acid synthase (DAPA synthase) variant R253K, S-adenosyl-(5')-3-methylthiopropylamine |
| 7,8-diaminopelargonic acid synthase (DAPA synthase)variant R253K | Escherichia coli | S-adenosyl-L-methionineapparent Km | 0.009 | 0.01 | 0.90 calc. | pH 9 | Open record: 7,8-diaminopelargonic acid synthase (DAPA synthase) variant R253K, S-adenosyl-L-methionine |
| 7,8-diaminopelargonic acid synthase (DAPA synthase)variant R253K | Escherichia coli | S-adenosyl-L-methionine | — | — | 0.9 | pH 9 | Open record: 7,8-diaminopelargonic acid synthase (DAPA synthase) variant R253K, S-adenosyl-L-methionine |
| 7,8-diaminopelargonic acid synthase (DAPA synthase)variant R253M | Escherichia coli | 7,8-diaminopelargonic acid | 0.67 | 6 × 10⁻⁴ | 1.1 × 10³ calc. | pH 9 | Open record: 7,8-diaminopelargonic acid synthase (DAPA synthase) variant R253M, 7,8-diaminopelargonic acid |
| 7,8-diaminopelargonic acid synthase (DAPA synthase)variant R253Q | Escherichia coli | 7,8-diaminopelargonic acid | 1.7 | 0.0014 | 1.2 × 10³ calc. | pH 9 | Open record: 7,8-diaminopelargonic acid synthase (DAPA synthase) variant R253Q, 7,8-diaminopelargonic acid |
| 7,8-diaminopelargonic acid synthase (DAPA synthase)variant R253Q | Escherichia coli | S-adenosyl-L-methionineapparent Km | 0.074 | 0.105 | 0.70 calc. | pH 9 | Open record: 7,8-diaminopelargonic acid synthase (DAPA synthase) variant R253Q, S-adenosyl-L-methionine |
| 7,8-diaminopelargonic acid synthase (DAPA synthase)variant R253Q | Escherichia coli | S-adenosyl-L-methionine | — | — | 0.7 | pH 9 | Open record: 7,8-diaminopelargonic acid synthase (DAPA synthase) variant R253Q, S-adenosyl-L-methionine |
| 7,8-diaminopelargonic acid synthase (DAPA synthase)variant Y144F | Escherichia coli | 7,8-diaminopelargonic acid | 0.04 | 0.15 | 0.27 calc. | pH 9 | Open record: 7,8-diaminopelargonic acid synthase (DAPA synthase) variant Y144F, 7,8-diaminopelargonic acid |
| 7,8-diaminopelargonic acid synthase (DAPA synthase)variant Y144F | Escherichia coli | S-adenosyl-L-methionine | — | — | 0.016 | pH 9 | Open record: 7,8-diaminopelargonic acid synthase (DAPA synthase) variant Y144F, S-adenosyl-L-methionine |
| 7,8-diaminopelargonic acid synthase (DAPA synthase)variant Y17F | Escherichia coli | 7,8-diaminopelargonic acid | 0.012 | 0.02 | 0.60 calc. | pH 9 | Open record: 7,8-diaminopelargonic acid synthase (DAPA synthase) variant Y17F, 7,8-diaminopelargonic acid |
| 7,8-diaminopelargonic acid synthase (DAPA synthase)variant Y17F | Escherichia coli | S-adenosyl-L-methionineapparent Km | 0.0035 | 0.063 | 0.056 calc. | pH 9 | Open record: 7,8-diaminopelargonic acid synthase (DAPA synthase) variant Y17F, S-adenosyl-L-methionine |
| 7,8-diaminopelargonic acid synthase (DAPA synthase)variant Y17F | Escherichia coli | S-adenosyl-L-methionine | — | — | 0.056 | pH 9 | Open record: 7,8-diaminopelargonic acid synthase (DAPA synthase) variant Y17F, S-adenosyl-L-methionine |