Values CatLog read from this paper
Conservation of mechanism in three chorismate-utilizing enzymes
PMID 14982443doi:10.1021/ja0389927Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 9 records (14 values) from this paper: 4-amino-4-deoxychorismate synthase (ADCS, PabB) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 5 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 4-amino-4-deoxychorismate synthase (ADCS, PabB) | Escherichia coli | ammonium | 0.032 | 145 | 2.2 × 10⁻⁴ calc. | pH 8.5, 25 °C | Open record: 4-amino-4-deoxychorismate synthase (ADCS, PabB), ammonium |
| 4-amino-4-deoxychorismate synthase (ADCS, PabB) | Escherichia coli | chorismatecosubstrate glutamine (PabA + Gln nitrogen source) | 0.53 | 0.013 | 41 calc. | pH 8.5, 25 °C | Open record: 4-amino-4-deoxychorismate synthase (ADCS, PabB), chorismate |
| 4-amino-4-deoxychorismate synthase (ADCS, PabB) | Escherichia coli | chorismate200 mM NH4+ as nitrogen source (100 mM (NH4)2SO4); no PabA | 0.032 | 0.058 | 0.55 calc. | pH 8.5, 25 °C | Open record: 4-amino-4-deoxychorismate synthase (ADCS, PabB), chorismate |
| 4-amino-4-deoxychorismate synthase (ADCS, PabB)variant K274A | Escherichia coli | ammonium | 0.033 | 169 | 2.0 × 10⁻⁴ calc. | pH 8.5, 25 °C | Open record: 4-amino-4-deoxychorismate synthase (ADCS, PabB) variant K274A, ammonium |
| 4-amino-4-deoxychorismate synthase (ADCS, PabB)variant K274A | Escherichia coli | chorismatePabA + glutamine as nitrogen source | 0.002 | — | — | pH 8.5, 25 °C | Open record: 4-amino-4-deoxychorismate synthase (ADCS, PabB) variant K274A, chorismate |
| 4-amino-4-deoxychorismate synthase (ADCS, PabB)variant K274A | Escherichia coli | chorismate200 mM NH4+ as nitrogen source (100 mM (NH4)2SO4); no PabA | 0.026 | 0.097 | 0.27 calc. | pH 8.5, 25 °C | Open record: 4-amino-4-deoxychorismate synthase (ADCS, PabB) variant K274A, chorismate |
| 4-amino-4-deoxychorismate synthase (ADCS, PabB)variant K274A | Escherichia coli | chorismatePabA + glutamine + 200 mM NH4+ as nitrogen sources | 0.01 | — | — | pH 8.5, 25 °C | Open record: 4-amino-4-deoxychorismate synthase (ADCS, PabB) variant K274A, chorismate |
| 4-amino-4-deoxychorismate synthase (ADCS, PabB)variant K274R | Escherichia coli | chorismatePabA + glutamine as nitrogen source | 8.3 × 10⁻⁴ | — | — | pH 8.5, 25 °C | Open record: 4-amino-4-deoxychorismate synthase (ADCS, PabB) variant K274R, chorismate |
| 4-amino-4-deoxychorismate synthase (ADCS, PabB)variant K274R | Escherichia coli | chorismate200 mM NH4+ as nitrogen source; no PabA | 9.8 × 10⁻⁵ | — | — | pH 8.5, 25 °C | Open record: 4-amino-4-deoxychorismate synthase (ADCS, PabB) variant K274R, chorismate |