Values CatLog read from this paper

The role of the allosteric B site in the fumarase reaction

PMID 14990798doi:10.1073/pnas.0307524101Open in Browse

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CatLog read 10 records (19 values) from this paper: fumarase C (FumC) from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 4.2.1.2

Records read from this paper
EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsOpen record
fumarase C (FumC)Escherichia colifumarate60—600pH 7.5, 25 °COpen record: fumarase C (FumC), fumarate
fumarase C (FumC)Escherichia colimalate129—430pH 7.5, 25 °COpen record: fumarase C (FumC), malate
fumarase C (FumC)variant H129NEscherichia colifumarate60—600pH 7.5, 25 °COpen record: fumarase C (FumC) variant H129N, fumarate
fumarase C (FumC)variant H129NEscherichia colimalate41—600pH 7.5, 25 °COpen record: fumarase C (FumC) variant H129N, malate
fumarase C (FumC)variant K127DEscherichia colifumarate68—750pH 7.5, 25 °COpen record: fumarase C (FumC) variant K127D, fumarate
fumarase C (FumC)variant K127DEscherichia colimalate180——pH 7.5, 25 °COpen record: fumarase C (FumC) variant K127D, malate
fumarase C (FumC)variant R126AEscherichia colifumarate22—50pH 7.5, 25 °COpen record: fumarase C (FumC) variant R126A, fumarate
fumarase C (FumC)variant R126AEscherichia colimalate30—33pH 7.5, 25 °COpen record: fumarase C (FumC) variant R126A, malate
fumarase C (FumC)variant R126A/H129NEscherichia colifumarate9.6—17pH 7.5, 25 °COpen record: fumarase C (FumC) variant R126A/H129N, fumarate
fumarase C (FumC)variant R126A/H129NEscherichia colimalate15—7.5pH 7.5, 25 °COpen record: fumarase C (FumC) variant R126A/H129N, malate