CatLogenzyme kinetics catalog

Values CatLog read from this paper

Rapid evolution of beta-glucuronidase specificity by saturation mutagenesis of an active site loop

PMID 15069062doi:10.1074/jbc.m401447200Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 10 records (26 values) from this paper: β-glucuronidase (GUS) from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.2.1.31

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
β-glucuronidase (GUS)Escherichia colip-nitrophenyl-β-D-glucuronide680.2340pH 7.6Open
β-glucuronidase (GUS)Escherichia colip-nitrophenyl-β-D-xylopyranoside0.0010.360.0029pH 7.6Open
β-glucuronidase (GUS)variant S557P/N566A/K568FEscherichia colip-nitrophenyl-β-D-glucuronide0.0420.180.24pH 7.6Open
β-glucuronidase (GUS)variant S557P/N566A/K568FEscherichia colip-nitrophenyl-β-D-xylopyranoside0.0630.110.59pH 7.6Open
β-glucuronidase (GUS)variant S557P/N566S/K568QEscherichia colip-nitrophenyl-β-D-glucuronide0.0120.220.054pH 7.6Open
β-glucuronidase (GUS)variant S557P/N566S/K568QEscherichia colip-nitrophenyl-β-D-xylopyranoside——0.24pH 7.6Open
β-glucuronidase (GUS)variant S557P/N566S/K568Q/S22N/G81S/K257E/T509A/Q598R/stop604WEscherichia colip-nitrophenyl-β-D-glucuronide0.0390.210.19pH 7.6Open
β-glucuronidase (GUS)variant S557P/N566S/K568Q/S22N/G81S/K257E/T509A/Q598R/stop604WEscherichia colip-nitrophenyl-β-D-xylopyranoside0.0540.660.081pH 7.6Open
β-glucuronidase (GUS)variant S557P/N566S/K568Q/V473A/G601SEscherichia colip-nitrophenyl-β-D-glucuronide0.0210.280.08pH 7.6Open
β-glucuronidase (GUS)variant S557P/N566S/K568Q/V473A/G601SEscherichia colip-nitrophenyl-β-D-xylopyranoside——0.19pH 7.6Open