EC 3.2.1.31: beta-glucuronidase
HydrolasesOpen in Browse
CatLog read 35 records for EC 3.2.1.31 from 4 papers, for 6 organisms. Names in these papers: β-glucuronidase, β-glucuronidase (GUS), beta-D-glucuronidase, BfGUS (beta-glucuronidase, 3CMG) and 3 more.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 45 records for EC 3.2.1.31 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.2.1.31 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Cloning and characterization of Thermotoga maritima beta-glucuronidase | Thermotoga maritima | 16 |
| Rapid evolution of beta-glucuronidase specificity by saturation mutagenesis of an active site loop | Escherichia coli | 10 |
| Effects of several alcohols on glycosidase activities in rat liver and brain | Rattus norvegicus | 5 |
| Structure and Inhibition of Microbiome β-Glucuronidases Essential to the Alleviation of Cancer Drug Toxicity | Bacteroides fragilis, Clostridium perfringens and 2 more | 4 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| beta-D-glucuronidase | Rattus norvegicus | 4-nitrophenyl beta-D-glucuronidecontrol rats (drank water only, same litter as treated rats) | — | 5.02 | — | pH 4.5, 37 °C | PMID 1499277 | Open record: beta-D-glucuronidase, 4-nitrophenyl beta-D-glucuronide |
| beta-D-glucuronidase | Rattus norvegicus | 4-nitrophenyl beta-D-glucuronide | — | 5.31 | — | pH 4.5, 37 °C | PMID 1499277 | Open record: beta-D-glucuronidase, 4-nitrophenyl beta-D-glucuronide |
| beta-D-glucuronidase | Rattus norvegicus | 4-nitrophenyl beta-D-glucuronide | — | 5.48 | — | pH 4.5, 37 °C | PMID 1499277 | Open record: beta-D-glucuronidase, 4-nitrophenyl beta-D-glucuronide |
| beta-D-glucuronidase | Rattus norvegicus | 4-nitrophenyl beta-D-glucuronide | — | 5 | — | pH 4.5, 37 °C | PMID 1499277 | Open record: beta-D-glucuronidase, 4-nitrophenyl beta-D-glucuronide |
| beta-D-glucuronidase | Rattus norvegicus | 4-nitrophenyl beta-D-glucuronide | — | 5.51 | — | pH 4.5, 37 °C | PMID 1499277 | Open record: beta-D-glucuronidase, 4-nitrophenyl beta-D-glucuronide |
| BfGUS (beta-glucuronidase, 3CMG) | Bacteroides fragilis | p-nitrophenyl glucuronide | 18 | 1.9 | 9.5 | 37 °C | PMID 26364932 | Open record: BfGUS (beta-glucuronidase, 3CMG), p-nitrophenyl glucuronide |
| CpGUS (beta-glucuronidase) | Clostridium perfringens | p-nitrophenyl glucuronide | 2.6 | 1.1 | 2.4 | 37 °C | PMID 26364932 | Open record: CpGUS (beta-glucuronidase), p-nitrophenyl glucuronide |
| EcGUS (beta-glucuronidase) | Escherichia coli | p-nitrophenyl glucuronide | 120 | 0.13 | 920 | 37 °C | PMID 26364932 | Open record: EcGUS (beta-glucuronidase), p-nitrophenyl glucuronide |
| SaGUS (beta-glucuronidase) | Streptococcus agalactiae | p-nitrophenyl glucuronide | 80 | 0.36 | 224 | 37 °C | PMID 26364932 | Open record: SaGUS (beta-glucuronidase), p-nitrophenyl glucuronide |
| β-glucuronidase | Thermotoga maritima | 4-nitrophenyl β-D-galactopyranosiduronic acid | 443 | 3.06 | 145 | pH 6.5, 75 °C | PMID 16303119 | Open record: β-glucuronidase, 4-nitrophenyl β-D-galactopyranosiduronic acid |
| β-glucuronidase | Thermotoga maritima | 4-nitrophenyl β-D-glucopyranoside | 9 | 145 | 0.06 | pH 6.5, 75 °C | PMID 16303119 | Open record: β-glucuronidase, 4-nitrophenyl β-D-glucopyranoside |
| β-glucuronidase | Thermotoga maritima | 4-nitrophenyl β-D-glucopyranosiduronic acid | 68 | 0.15 | 453 | pH 6.5, 75 °C | PMID 16303119 | Open record: β-glucuronidase, 4-nitrophenyl β-D-glucopyranosiduronic acid |
| β-glucuronidase | Thermotoga maritima | 4-nitrophenyl β-D-glucopyranosiduronic acid | 29 | 0.083 | 352 | pH 6.5, 65 °C | PMID 16303119 | Open record: β-glucuronidase, 4-nitrophenyl β-D-glucopyranosiduronic acid |
| β-glucuronidasevariant E383A | Thermotoga maritima | 4-nitrophenyl β-D-glucopyranosiduronic acidapparent kcat | 0.33 | — | — | pH 6.5, 65 °C | PMID 16303119 | Open record: β-glucuronidase variant E383A, 4-nitrophenyl β-D-glucopyranosiduronic acid |
| β-glucuronidasevariant E383A | Thermotoga maritima | 4-nitrophenyl β-D-glucopyranosiduronic acidapparent Km | — | 0.0065 | — | pH 6.5, 65 °C | PMID 16303119 | Open record: β-glucuronidase variant E383A, 4-nitrophenyl β-D-glucopyranosiduronic acid |
| β-glucuronidasevariant E383A | Thermotoga maritima | 4-nitrophenyl β-D-glucopyranosiduronic acidapparent kcat/Km | — | — | 51 | pH 6.5, 65 °C | PMID 16303119 | Open record: β-glucuronidase variant E383A, 4-nitrophenyl β-D-glucopyranosiduronic acid |
| β-glucuronidasevariant E383A | Thermotoga maritima | 4-nitrophenyl β-D-glucopyranosiduronic acidwith 100 mM sodium azide (activity rescue); apparent kcat | 0.46 | — | — | pH 6.5, 65 °C | PMID 16303119 | Open record: β-glucuronidase variant E383A, 4-nitrophenyl β-D-glucopyranosiduronic acid |
| β-glucuronidasevariant E383A | Thermotoga maritima | 4-nitrophenyl β-D-glucopyranosiduronic acidwith 100 mM sodium azide (activity rescue); apparent Km | — | 0.012 | — | pH 6.5, 65 °C | PMID 16303119 | Open record: β-glucuronidase variant E383A, 4-nitrophenyl β-D-glucopyranosiduronic acid |
| β-glucuronidasevariant E383A | Thermotoga maritima | 4-nitrophenyl β-D-glucopyranosiduronic acidwith 100 mM sodium azide (activity rescue); apparent kcat/Km | — | — | 40 | pH 6.5, 65 °C | PMID 16303119 | Open record: β-glucuronidase variant E383A, 4-nitrophenyl β-D-glucopyranosiduronic acid |
| β-glucuronidasevariant E383Q | Thermotoga maritima | 4-nitrophenyl β-D-glucopyranosiduronic acidapparent kcat | 0.98 | — | — | pH 6.5, 65 °C | PMID 16303119 | Open record: β-glucuronidase variant E383Q, 4-nitrophenyl β-D-glucopyranosiduronic acid |
| β-glucuronidasevariant E383Q | Thermotoga maritima | 4-nitrophenyl β-D-glucopyranosiduronic acidapparent Km | — | 0.0046 | — | pH 6.5, 65 °C | PMID 16303119 | Open record: β-glucuronidase variant E383Q, 4-nitrophenyl β-D-glucopyranosiduronic acid |
| β-glucuronidasevariant E383Q | Thermotoga maritima | 4-nitrophenyl β-D-glucopyranosiduronic acidapparent kcat/Km | — | — | 213 | pH 6.5, 65 °C | PMID 16303119 | Open record: β-glucuronidase variant E383Q, 4-nitrophenyl β-D-glucopyranosiduronic acid |
| β-glucuronidasevariant E383Q | Thermotoga maritima | 4-nitrophenyl β-D-glucopyranosiduronic acidwith 100 mM sodium azide (activity rescue); apparent kcat | 11 | — | — | pH 6.5, 65 °C | PMID 16303119 | Open record: β-glucuronidase variant E383Q, 4-nitrophenyl β-D-glucopyranosiduronic acid |
| β-glucuronidasevariant E383Q | Thermotoga maritima | 4-nitrophenyl β-D-glucopyranosiduronic acidwith 100 mM sodium azide (activity rescue); apparent Km | — | 0.069 | — | pH 6.5, 65 °C | PMID 16303119 | Open record: β-glucuronidase variant E383Q, 4-nitrophenyl β-D-glucopyranosiduronic acid |
| β-glucuronidasevariant E383Q | Thermotoga maritima | 4-nitrophenyl β-D-glucopyranosiduronic acidwith 100 mM sodium azide (activity rescue); apparent kcat/Km | — | — | 160 | pH 6.5, 65 °C | PMID 16303119 | Open record: β-glucuronidase variant E383Q, 4-nitrophenyl β-D-glucopyranosiduronic acid |
| β-glucuronidase (GUS) | Escherichia coli | p-nitrophenyl-β-D-glucuronide | 68 | 0.2 | 340 | pH 7.6 | PMID 15069062 | Open record: β-glucuronidase (GUS), p-nitrophenyl-β-D-glucuronide |
| β-glucuronidase (GUS) | Escherichia coli | p-nitrophenyl-β-D-xylopyranoside | 0.001 | 0.36 | 0.0029 | pH 7.6 | PMID 15069062 | Open record: β-glucuronidase (GUS), p-nitrophenyl-β-D-xylopyranoside |
| β-glucuronidase (GUS)variant S557P/N566A/K568F | Escherichia coli | p-nitrophenyl-β-D-glucuronide | 0.042 | 0.18 | 0.24 | pH 7.6 | PMID 15069062 | Open record: β-glucuronidase (GUS) variant S557P/N566A/K568F, p-nitrophenyl-β-D-glucuronide |
| β-glucuronidase (GUS)variant S557P/N566A/K568F | Escherichia coli | p-nitrophenyl-β-D-xylopyranoside | 0.063 | 0.11 | 0.59 | pH 7.6 | PMID 15069062 | Open record: β-glucuronidase (GUS) variant S557P/N566A/K568F, p-nitrophenyl-β-D-xylopyranoside |
| β-glucuronidase (GUS)variant S557P/N566S/K568Q | Escherichia coli | p-nitrophenyl-β-D-glucuronide | 0.012 | 0.22 | 0.054 | pH 7.6 | PMID 15069062 | Open record: β-glucuronidase (GUS) variant S557P/N566S/K568Q, p-nitrophenyl-β-D-glucuronide |
| β-glucuronidase (GUS)variant S557P/N566S/K568Q | Escherichia coli | p-nitrophenyl-β-D-xylopyranoside | — | — | 0.24 | pH 7.6 | PMID 15069062 | Open record: β-glucuronidase (GUS) variant S557P/N566S/K568Q, p-nitrophenyl-β-D-xylopyranoside |
| β-glucuronidase (GUS)variant S557P/N566S/K568Q/S22N/G81S/K257E/T509A/Q598R/stop604W | Escherichia coli | p-nitrophenyl-β-D-glucuronide | 0.039 | 0.21 | 0.19 | pH 7.6 | PMID 15069062 | Open record: β-glucuronidase (GUS) variant S557P/N566S/K568Q/S22N/G81S/K257E/T509A/Q598R/stop604W, p-nitrophenyl-β-D-glucuronide |
| β-glucuronidase (GUS)variant S557P/N566S/K568Q/S22N/G81S/K257E/T509A/Q598R/stop604W | Escherichia coli | p-nitrophenyl-β-D-xylopyranoside | 0.054 | 0.66 | 0.081 | pH 7.6 | PMID 15069062 | Open record: β-glucuronidase (GUS) variant S557P/N566S/K568Q/S22N/G81S/K257E/T509A/Q598R/stop604W, p-nitrophenyl-β-D-xylopyranoside |
| β-glucuronidase (GUS)variant S557P/N566S/K568Q/V473A/G601S | Escherichia coli | p-nitrophenyl-β-D-glucuronide | 0.021 | 0.28 | 0.08 | pH 7.6 | PMID 15069062 | Open record: β-glucuronidase (GUS) variant S557P/N566S/K568Q/V473A/G601S, p-nitrophenyl-β-D-glucuronide |
| β-glucuronidase (GUS)variant S557P/N566S/K568Q/V473A/G601S | Escherichia coli | p-nitrophenyl-β-D-xylopyranoside | — | — | 0.19 | pH 7.6 | PMID 15069062 | Open record: β-glucuronidase (GUS) variant S557P/N566S/K568Q/V473A/G601S, p-nitrophenyl-β-D-xylopyranoside |