Values CatLog read from this paper
Cloning and characterization of Thermotoga maritima beta-glucuronidase
PMID 16303119doi:10.1016/j.carres.2005.10.005Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 16 records (24 values) from this paper: β-glucuronidase from Thermotoga maritima.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| β-glucuronidase | Thermotoga maritima | 4-nitrophenyl β-D-galactopyranosiduronic acid | 443 | 3.06 | 145 | pH 6.5, 75 °C | Open record: β-glucuronidase, 4-nitrophenyl β-D-galactopyranosiduronic acid |
| β-glucuronidase | Thermotoga maritima | 4-nitrophenyl β-D-glucopyranoside | 9 | 145 | 0.06 | pH 6.5, 75 °C | Open record: β-glucuronidase, 4-nitrophenyl β-D-glucopyranoside |
| β-glucuronidase | Thermotoga maritima | 4-nitrophenyl β-D-glucopyranosiduronic acid | 68 | 0.15 | 453 | pH 6.5, 75 °C | Open record: β-glucuronidase, 4-nitrophenyl β-D-glucopyranosiduronic acid |
| β-glucuronidase | Thermotoga maritima | 4-nitrophenyl β-D-glucopyranosiduronic acid | 29 | 0.083 | 352 | pH 6.5, 65 °C | Open record: β-glucuronidase, 4-nitrophenyl β-D-glucopyranosiduronic acid |
| β-glucuronidasevariant E383A | Thermotoga maritima | 4-nitrophenyl β-D-glucopyranosiduronic acidapparent kcat | 0.33 | — | — | pH 6.5, 65 °C | Open record: β-glucuronidase variant E383A, 4-nitrophenyl β-D-glucopyranosiduronic acid |
| β-glucuronidasevariant E383A | Thermotoga maritima | 4-nitrophenyl β-D-glucopyranosiduronic acidapparent Km | — | 0.0065 | — | pH 6.5, 65 °C | Open record: β-glucuronidase variant E383A, 4-nitrophenyl β-D-glucopyranosiduronic acid |
| β-glucuronidasevariant E383A | Thermotoga maritima | 4-nitrophenyl β-D-glucopyranosiduronic acidapparent kcat/Km | — | — | 51 | pH 6.5, 65 °C | Open record: β-glucuronidase variant E383A, 4-nitrophenyl β-D-glucopyranosiduronic acid |
| β-glucuronidasevariant E383A | Thermotoga maritima | 4-nitrophenyl β-D-glucopyranosiduronic acidwith 100 mM sodium azide (activity rescue); apparent kcat | 0.46 | — | — | pH 6.5, 65 °C | Open record: β-glucuronidase variant E383A, 4-nitrophenyl β-D-glucopyranosiduronic acid |
| β-glucuronidasevariant E383A | Thermotoga maritima | 4-nitrophenyl β-D-glucopyranosiduronic acidwith 100 mM sodium azide (activity rescue); apparent Km | — | 0.012 | — | pH 6.5, 65 °C | Open record: β-glucuronidase variant E383A, 4-nitrophenyl β-D-glucopyranosiduronic acid |
| β-glucuronidasevariant E383A | Thermotoga maritima | 4-nitrophenyl β-D-glucopyranosiduronic acidwith 100 mM sodium azide (activity rescue); apparent kcat/Km | — | — | 40 | pH 6.5, 65 °C | Open record: β-glucuronidase variant E383A, 4-nitrophenyl β-D-glucopyranosiduronic acid |
| β-glucuronidasevariant E383Q | Thermotoga maritima | 4-nitrophenyl β-D-glucopyranosiduronic acidapparent kcat | 0.98 | — | — | pH 6.5, 65 °C | Open record: β-glucuronidase variant E383Q, 4-nitrophenyl β-D-glucopyranosiduronic acid |
| β-glucuronidasevariant E383Q | Thermotoga maritima | 4-nitrophenyl β-D-glucopyranosiduronic acidapparent Km | — | 0.0046 | — | pH 6.5, 65 °C | Open record: β-glucuronidase variant E383Q, 4-nitrophenyl β-D-glucopyranosiduronic acid |
| β-glucuronidasevariant E383Q | Thermotoga maritima | 4-nitrophenyl β-D-glucopyranosiduronic acidapparent kcat/Km | — | — | 213 | pH 6.5, 65 °C | Open record: β-glucuronidase variant E383Q, 4-nitrophenyl β-D-glucopyranosiduronic acid |
| β-glucuronidasevariant E383Q | Thermotoga maritima | 4-nitrophenyl β-D-glucopyranosiduronic acidwith 100 mM sodium azide (activity rescue); apparent kcat | 11 | — | — | pH 6.5, 65 °C | Open record: β-glucuronidase variant E383Q, 4-nitrophenyl β-D-glucopyranosiduronic acid |
| β-glucuronidasevariant E383Q | Thermotoga maritima | 4-nitrophenyl β-D-glucopyranosiduronic acidwith 100 mM sodium azide (activity rescue); apparent Km | — | 0.069 | — | pH 6.5, 65 °C | Open record: β-glucuronidase variant E383Q, 4-nitrophenyl β-D-glucopyranosiduronic acid |
| β-glucuronidasevariant E383Q | Thermotoga maritima | 4-nitrophenyl β-D-glucopyranosiduronic acidwith 100 mM sodium azide (activity rescue); apparent kcat/Km | — | — | 160 | pH 6.5, 65 °C | Open record: β-glucuronidase variant E383Q, 4-nitrophenyl β-D-glucopyranosiduronic acid |