Values CatLog read from this paper
Substitution of the conserved Arg-Tyr dyad selectively disrupts the hydrolysis phase of the IMP dehydrogenase reaction
PMID 15078097doi:10.1021/bi035823qOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 4 records (6 values) from this paper: IMP dehydrogenase (IMPDH) from Tritrichomonas foetus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| IMP dehydrogenase (IMPDH)variant R418A | Tritrichomonas foetus | inosine 5'-monophosphate | — | 0.003 | — | pH 8, 25 °C | Open record: IMP dehydrogenase (IMPDH) variant R418A, inosine 5'-monophosphate |
| IMP dehydrogenase (IMPDH)variant R418A | Tritrichomonas foetus | nicotinamide adenine dinucleotide | 0.004 | 0.0016 | 2.5 calc. | pH 8, 25 °C | Open record: IMP dehydrogenase (IMPDH) variant R418A, nicotinamide adenine dinucleotide |
| IMP dehydrogenase (IMPDH)variant Y419F | Tritrichomonas foetus | inosine 5'-monophosphate | — | 7 × 10⁻⁴ | — | pH 8, 25 °C | Open record: IMP dehydrogenase (IMPDH) variant Y419F, inosine 5'-monophosphate |
| IMP dehydrogenase (IMPDH)variant Y419F | Tritrichomonas foetus | nicotinamide adenine dinucleotide | 0.22 | 0.07 | 3.1 calc. | pH 8, 25 °C | Open record: IMP dehydrogenase (IMPDH) variant Y419F, nicotinamide adenine dinucleotide |