Values CatLog read from this paper
Essential role of histidine 20 in the catalytic mechanism of Escherichia coli peptidyl-tRNA hydrolase
PMID 15078105doi:10.1021/bi0302200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 11 records (33 values) from this paper: peptidyl-tRNA hydrolase (Pth) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| peptidyl-tRNA hydrolase (Pth) | Escherichia coli | Oregon Green 488-X-labelled methionyl-tRNAMet(m) | 9.3 | 0.0055 | 1.7 × 10³ | pH 7.5 | Open record: peptidyl-tRNA hydrolase (Pth), Oregon Green 488-X-labelled methionyl-tRNAMet(m) |
| peptidyl-tRNA hydrolase (Pth)variant D93N (Asp93Asn) | Escherichia coli | Oregon Green 488-X-labelled methionyl-tRNAMet(m) | 0.37 | 0.0038 | 100 | pH 7.5 | Open record: peptidyl-tRNA hydrolase (Pth) variant D93N (Asp93Asn), Oregon Green 488-X-labelled methionyl-tRNAMet(m) |
| peptidyl-tRNA hydrolase (Pth)variant H113Q (His113Gln) | Escherichia coli | Oregon Green 488-X-labelled methionyl-tRNAMet(m) | 9.1 | 0.024 | 380 | pH 7.5 | Open record: peptidyl-tRNA hydrolase (Pth) variant H113Q (His113Gln), Oregon Green 488-X-labelled methionyl-tRNAMet(m) |
| peptidyl-tRNA hydrolase (Pth)variant K103Q (Lys103Gln) | Escherichia coli | Oregon Green 488-X-labelled methionyl-tRNAMet(m) | 5 | 0.023 | 220 | pH 7.5 | Open record: peptidyl-tRNA hydrolase (Pth) variant K103Q (Lys103Gln), Oregon Green 488-X-labelled methionyl-tRNAMet(m) |
| peptidyl-tRNA hydrolase (Pth)variant K103R (Lys103Arg) | Escherichia coli | Oregon Green 488-X-labelled methionyl-tRNAMet(m) | 6.3 | 0.0041 | 1.5 × 10³ | pH 7.5 | Open record: peptidyl-tRNA hydrolase (Pth) variant K103R (Lys103Arg), Oregon Green 488-X-labelled methionyl-tRNAMet(m) |
| peptidyl-tRNA hydrolase (Pth)variant K103S (Lys103Ser) | Escherichia coli | Oregon Green 488-X-labelled methionyl-tRNAMet(m) | 2.6 | 0.037 | 70 | pH 7.5 | Open record: peptidyl-tRNA hydrolase (Pth) variant K103S (Lys103Ser), Oregon Green 488-X-labelled methionyl-tRNAMet(m) |
| peptidyl-tRNA hydrolase (Pth)variant K105Q (Lys105Gln) | Escherichia coli | Oregon Green 488-X-labelled methionyl-tRNAMet(m) | 1.9 | 0.036 | 53 | pH 7.5 | Open record: peptidyl-tRNA hydrolase (Pth) variant K105Q (Lys105Gln), Oregon Green 488-X-labelled methionyl-tRNAMet(m) |
| peptidyl-tRNA hydrolase (Pth)variant K105R (Lys105Arg) | Escherichia coli | Oregon Green 488-X-labelled methionyl-tRNAMet(m) | 2.4 | 0.0087 | 280 | pH 7.5 | Open record: peptidyl-tRNA hydrolase (Pth) variant K105R (Lys105Arg), Oregon Green 488-X-labelled methionyl-tRNAMet(m) |
| peptidyl-tRNA hydrolase (Pth)variant K105S (Lys105Ser) | Escherichia coli | Oregon Green 488-X-labelled methionyl-tRNAMet(m) | 1.5 | 0.03 | 50 | pH 7.5 | Open record: peptidyl-tRNA hydrolase (Pth) variant K105S (Lys105Ser), Oregon Green 488-X-labelled methionyl-tRNAMet(m) |
| peptidyl-tRNA hydrolase (Pth)variant M67E (Met67Glu) | Escherichia coli | Oregon Green 488-X-labelled methionyl-tRNAMet(m) | 0.045 | 0.0036 | 13 | pH 7.5 | Open record: peptidyl-tRNA hydrolase (Pth) variant M67E (Met67Glu), Oregon Green 488-X-labelled methionyl-tRNAMet(m) |
| peptidyl-tRNA hydrolase (Pth)variant N10D (Asn10Asp) | Escherichia coli | Oregon Green 488-X-labelled methionyl-tRNAMet(m) | 0.005 | 0.0079 | 0.6 | pH 7.5 | Open record: peptidyl-tRNA hydrolase (Pth) variant N10D (Asn10Asp), Oregon Green 488-X-labelled methionyl-tRNAMet(m) |