EC 3.1.1.29: peptidyl-tRNA hydrolase
HydrolasesOpen in Browse
CatLog read 32 records for EC 3.1.1.29 from 4 papers, for 3 organisms. Names in these papers: peptidyl-tRNA hydrolase (Pth), peptidyl-tRNA hydrolase (suPth2), peptidyl-tRNA hydrolase (PTH, archaeal-type), peptidyl-tRNA hydrolase (TtPth).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 32 records for EC 3.1.1.29 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.1.1.29 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Essential role of histidine 20 in the catalytic mechanism of Escherichia coli peptidyl-tRNA hydrolase | Escherichia coli | 11 |
| Crystal structure at 1.8 A resolution and identification of active site residues of Sulfolobus solfataricus peptidyl-tRNA hydrolase | Sulfolobus solfataricus | 11 |
| Peptidyl-tRNA hydrolase from Sulfolobus solfataricus | Sulfolobus solfataricus | 5 |
| Binding mode between peptidyl-tRNA hydrolase and the peptidyl-A76 moiety of the substrate | Thermus thermophilus | 5 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| peptidyl-tRNA hydrolase (Pth) | Escherichia coli | Oregon Green 488-X-labelled methionyl-tRNAMet(m) | 9.3 | 0.0055 | 1.7 × 10³ | pH 7.5 | PMID 15078105 | Open record: peptidyl-tRNA hydrolase (Pth), Oregon Green 488-X-labelled methionyl-tRNAMet(m) |
| peptidyl-tRNA hydrolase (Pth)variant D93N (Asp93Asn) | Escherichia coli | Oregon Green 488-X-labelled methionyl-tRNAMet(m) | 0.37 | 0.0038 | 100 | pH 7.5 | PMID 15078105 | Open record: peptidyl-tRNA hydrolase (Pth) variant D93N (Asp93Asn), Oregon Green 488-X-labelled methionyl-tRNAMet(m) |
| peptidyl-tRNA hydrolase (Pth)variant H113Q (His113Gln) | Escherichia coli | Oregon Green 488-X-labelled methionyl-tRNAMet(m) | 9.1 | 0.024 | 380 | pH 7.5 | PMID 15078105 | Open record: peptidyl-tRNA hydrolase (Pth) variant H113Q (His113Gln), Oregon Green 488-X-labelled methionyl-tRNAMet(m) |
| peptidyl-tRNA hydrolase (Pth)variant K103Q (Lys103Gln) | Escherichia coli | Oregon Green 488-X-labelled methionyl-tRNAMet(m) | 5 | 0.023 | 220 | pH 7.5 | PMID 15078105 | Open record: peptidyl-tRNA hydrolase (Pth) variant K103Q (Lys103Gln), Oregon Green 488-X-labelled methionyl-tRNAMet(m) |
| peptidyl-tRNA hydrolase (Pth)variant K103R (Lys103Arg) | Escherichia coli | Oregon Green 488-X-labelled methionyl-tRNAMet(m) | 6.3 | 0.0041 | 1.5 × 10³ | pH 7.5 | PMID 15078105 | Open record: peptidyl-tRNA hydrolase (Pth) variant K103R (Lys103Arg), Oregon Green 488-X-labelled methionyl-tRNAMet(m) |
| peptidyl-tRNA hydrolase (Pth)variant K103S (Lys103Ser) | Escherichia coli | Oregon Green 488-X-labelled methionyl-tRNAMet(m) | 2.6 | 0.037 | 70 | pH 7.5 | PMID 15078105 | Open record: peptidyl-tRNA hydrolase (Pth) variant K103S (Lys103Ser), Oregon Green 488-X-labelled methionyl-tRNAMet(m) |
| peptidyl-tRNA hydrolase (Pth)variant K105Q (Lys105Gln) | Escherichia coli | Oregon Green 488-X-labelled methionyl-tRNAMet(m) | 1.9 | 0.036 | 53 | pH 7.5 | PMID 15078105 | Open record: peptidyl-tRNA hydrolase (Pth) variant K105Q (Lys105Gln), Oregon Green 488-X-labelled methionyl-tRNAMet(m) |
| peptidyl-tRNA hydrolase (Pth)variant K105R (Lys105Arg) | Escherichia coli | Oregon Green 488-X-labelled methionyl-tRNAMet(m) | 2.4 | 0.0087 | 280 | pH 7.5 | PMID 15078105 | Open record: peptidyl-tRNA hydrolase (Pth) variant K105R (Lys105Arg), Oregon Green 488-X-labelled methionyl-tRNAMet(m) |
| peptidyl-tRNA hydrolase (Pth)variant K105S (Lys105Ser) | Escherichia coli | Oregon Green 488-X-labelled methionyl-tRNAMet(m) | 1.5 | 0.03 | 50 | pH 7.5 | PMID 15078105 | Open record: peptidyl-tRNA hydrolase (Pth) variant K105S (Lys105Ser), Oregon Green 488-X-labelled methionyl-tRNAMet(m) |
| peptidyl-tRNA hydrolase (Pth)variant M67E (Met67Glu) | Escherichia coli | Oregon Green 488-X-labelled methionyl-tRNAMet(m) | 0.045 | 0.0036 | 13 | pH 7.5 | PMID 15078105 | Open record: peptidyl-tRNA hydrolase (Pth) variant M67E (Met67Glu), Oregon Green 488-X-labelled methionyl-tRNAMet(m) |
| peptidyl-tRNA hydrolase (Pth)variant N10D (Asn10Asp) | Escherichia coli | Oregon Green 488-X-labelled methionyl-tRNAMet(m) | 0.005 | 0.0079 | 0.6 | pH 7.5 | PMID 15078105 | Open record: peptidyl-tRNA hydrolase (Pth) variant N10D (Asn10Asp), Oregon Green 488-X-labelled methionyl-tRNAMet(m) |
| peptidyl-tRNA hydrolase (PTH, archaeal-type) | Sulfolobus solfataricus | Acetyl-histidyl-tRNAHis | 3.4 | 1.6 × 10⁻⁵ | 2.1 × 10⁵ calc. | pH 7.5, 50 °C | PMID 12799450 | Open record: peptidyl-tRNA hydrolase (PTH, archaeal-type), Acetyl-histidyl-tRNAHis |
| peptidyl-tRNA hydrolase (PTH, archaeal-type) | Sulfolobus solfataricus | Diacetyl-lysyl-tRNALys | 1.8 | 1.1 × 10⁻⁵ | 1.6 × 10⁵ calc. | pH 7.5, 50 °C | PMID 12799450 | Open record: peptidyl-tRNA hydrolase (PTH, archaeal-type), Diacetyl-lysyl-tRNALys |
| peptidyl-tRNA hydrolase (PTH, archaeal-type) | Sulfolobus solfataricus | Diacetyl-lysyl-tRNALys (dephosphorylated) | 0.86 | 2.8 × 10⁻⁶ | 3.1 × 10⁵ calc. | pH 7.5, 50 °C | PMID 12799450 | Open record: peptidyl-tRNA hydrolase (PTH, archaeal-type), Diacetyl-lysyl-tRNALys (dephosphorylated) |
| peptidyl-tRNA hydrolase (PTH, archaeal-type) | Sulfolobus solfataricus | Formyl-methionyl-tRNAfMet | 3 | 1.2 × 10⁻⁵ | 2.5 × 10⁵ calc. | pH 7.5, 50 °C | PMID 12799450 | Open record: peptidyl-tRNA hydrolase (PTH, archaeal-type), Formyl-methionyl-tRNAfMet |
| peptidyl-tRNA hydrolase (PTH, archaeal-type) | Sulfolobus solfataricus | Formyl-methionyl-tRNAfMet (dephosphorylated) | 3 | 3 × 10⁻⁵ | 1.0 × 10⁵ calc. | pH 7.5, 50 °C | PMID 12799450 | Open record: peptidyl-tRNA hydrolase (PTH, archaeal-type), Formyl-methionyl-tRNAfMet (dephosphorylated) |
| peptidyl-tRNA hydrolase (suPth2) | Sulfolobus solfataricus | E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys) | 1.8 | 9.1 × 10⁻⁶ | 2.0 × 10⁵ calc. | pH 7.5, 50 °C | PMID 15766258 | Open record: peptidyl-tRNA hydrolase (suPth2), E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys) |
| peptidyl-tRNA hydrolase (suPth2)variant D86A | Sulfolobus solfataricus | E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys) | 0.0025 | 5.4 × 10⁻⁶ | 460 calc. | pH 7.5, 50 °C | PMID 15766258 | Open record: peptidyl-tRNA hydrolase (suPth2) variant D86A, E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys) |
| peptidyl-tRNA hydrolase (suPth2)variant D86A/K18A | Sulfolobus solfataricus | E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys) | 0.002 | 6.7 × 10⁻⁶ | 300 calc. | pH 7.5, 50 °C | PMID 15766258 | Open record: peptidyl-tRNA hydrolase (suPth2) variant D86A/K18A, E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys) |
| peptidyl-tRNA hydrolase (suPth2)variant H25A | Sulfolobus solfataricus | E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys) | 0.1 | 4.4 × 10⁻⁶ | 2.3 × 10⁴ calc. | pH 7.5, 50 °C | PMID 15766258 | Open record: peptidyl-tRNA hydrolase (suPth2) variant H25A, E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys) |
| peptidyl-tRNA hydrolase (suPth2)variant K118A | Sulfolobus solfataricus | E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys) | 2.2 | 1.28 × 10⁻⁵ | 1.7 × 10⁵ calc. | pH 7.5, 50 °C | PMID 15766258 | Open record: peptidyl-tRNA hydrolase (suPth2) variant K118A, E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys) |
| peptidyl-tRNA hydrolase (suPth2)variant K18A | Sulfolobus solfataricus | E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys) | 0.002 | 5.3 × 10⁻⁶ | 380 calc. | pH 7.5, 50 °C | PMID 15766258 | Open record: peptidyl-tRNA hydrolase (suPth2) variant K18A, E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys) |
| peptidyl-tRNA hydrolase (suPth2)variant K56A | Sulfolobus solfataricus | E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys) | 0.07 | 3.2 × 10⁻⁶ | 2.2 × 10⁴ calc. | pH 7.5, 50 °C | PMID 15766258 | Open record: peptidyl-tRNA hydrolase (suPth2) variant K56A, E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys) |
| peptidyl-tRNA hydrolase (suPth2)variant Q22A | Sulfolobus solfataricus | E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys) | 0.06 | 1.6 × 10⁻⁶ | 3.8 × 10⁴ calc. | pH 7.5, 50 °C | PMID 15766258 | Open record: peptidyl-tRNA hydrolase (suPth2) variant Q22A, E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys) |
| peptidyl-tRNA hydrolase (suPth2)variant Q54A | Sulfolobus solfataricus | E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys) | 0.27 | 2.9 × 10⁻⁶ | 9.3 × 10⁴ calc. | pH 7.5, 50 °C | PMID 15766258 | Open record: peptidyl-tRNA hydrolase (suPth2) variant Q54A, E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys) |
| peptidyl-tRNA hydrolase (suPth2)variant T90A | Sulfolobus solfataricus | E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys) | 0.02 | 7.2 × 10⁻⁶ | 2.8 × 10³ calc. | pH 7.5, 50 °C | PMID 15766258 | Open record: peptidyl-tRNA hydrolase (suPth2) variant T90A, E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys) |
| peptidyl-tRNA hydrolase (suPth2)variant T98A | Sulfolobus solfataricus | E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys) | 0.06 | 9 × 10⁻⁷ | 6.7 × 10⁴ calc. | pH 7.5, 50 °C | PMID 15766258 | Open record: peptidyl-tRNA hydrolase (suPth2) variant T98A, E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys) |
| peptidyl-tRNA hydrolase (TtPth) | Thermus thermophilus | diacetyl-lysyl-tRNA^{Lys} | 0.9 | 0.0023 | 390 calc. | PMID 40049414 | Open record: peptidyl-tRNA hydrolase (TtPth), diacetyl-lysyl-tRNA^{Lys} | |
| peptidyl-tRNA hydrolase (TtPth)variant E83A | Thermus thermophilus | diacetyl-lysyl-tRNA^{Lys} | 0.024 | 0.0035 | 6.9 calc. | PMID 40049414 | Open record: peptidyl-tRNA hydrolase (TtPth) variant E83A, diacetyl-lysyl-tRNA^{Lys} | |
| peptidyl-tRNA hydrolase (TtPth)variant L138A | Thermus thermophilus | diacetyl-lysyl-tRNA^{Lys} | 0.7 | 0.0032 | 220 calc. | PMID 40049414 | Open record: peptidyl-tRNA hydrolase (TtPth) variant L138A, diacetyl-lysyl-tRNA^{Lys} | |
| peptidyl-tRNA hydrolase (TtPth)variant N102A | Thermus thermophilus | diacetyl-lysyl-tRNA^{Lys} | 0.029 | 0.0053 | 5.5 calc. | PMID 40049414 | Open record: peptidyl-tRNA hydrolase (TtPth) variant N102A, diacetyl-lysyl-tRNA^{Lys} | |
| peptidyl-tRNA hydrolase (TtPth)variant R103A | Thermus thermophilus | diacetyl-lysyl-tRNA^{Lys} | 0.12 | 0.0049 | 24 calc. | PMID 40049414 | Open record: peptidyl-tRNA hydrolase (TtPth) variant R103A, diacetyl-lysyl-tRNA^{Lys} |