Values CatLog read from this paper

Crystal structure at 1.8 A resolution and identification of active site residues of Sulfolobus solfataricus peptidyl-tRNA hydrolase

PMID 15766258doi:10.1021/bi047711kOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 11 records (22 values) from this paper: peptidyl-tRNA hydrolase (suPth2) from Sulfolobus solfataricus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 11 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 3.1.1.29

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
peptidyl-tRNA hydrolase (suPth2)	Sulfolobus solfataricus	E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys)	1.8	9.1 × 10⁻⁶	2.0 × 10⁵ calc.	pH 7.5, 50 °C	Open record: peptidyl-tRNA hydrolase (suPth2), E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys)
peptidyl-tRNA hydrolase (suPth2)variant D86A	Sulfolobus solfataricus	E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys)	0.0025	5.4 × 10⁻⁶	460 calc.	pH 7.5, 50 °C	Open record: peptidyl-tRNA hydrolase (suPth2) variant D86A, E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys)
peptidyl-tRNA hydrolase (suPth2)variant D86A/K18A	Sulfolobus solfataricus	E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys)	0.002	6.7 × 10⁻⁶	300 calc.	pH 7.5, 50 °C	Open record: peptidyl-tRNA hydrolase (suPth2) variant D86A/K18A, E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys)
peptidyl-tRNA hydrolase (suPth2)variant H25A	Sulfolobus solfataricus	E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys)	0.1	4.4 × 10⁻⁶	2.3 × 10⁴ calc.	pH 7.5, 50 °C	Open record: peptidyl-tRNA hydrolase (suPth2) variant H25A, E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys)
peptidyl-tRNA hydrolase (suPth2)variant K118A	Sulfolobus solfataricus	E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys)	2.2	1.28 × 10⁻⁵	1.7 × 10⁵ calc.	pH 7.5, 50 °C	Open record: peptidyl-tRNA hydrolase (suPth2) variant K118A, E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys)
peptidyl-tRNA hydrolase (suPth2)variant K18A	Sulfolobus solfataricus	E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys)	0.002	5.3 × 10⁻⁶	380 calc.	pH 7.5, 50 °C	Open record: peptidyl-tRNA hydrolase (suPth2) variant K18A, E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys)
peptidyl-tRNA hydrolase (suPth2)variant K56A	Sulfolobus solfataricus	E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys)	0.07	3.2 × 10⁻⁶	2.2 × 10⁴ calc.	pH 7.5, 50 °C	Open record: peptidyl-tRNA hydrolase (suPth2) variant K56A, E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys)
peptidyl-tRNA hydrolase (suPth2)variant Q22A	Sulfolobus solfataricus	E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys)	0.06	1.6 × 10⁻⁶	3.8 × 10⁴ calc.	pH 7.5, 50 °C	Open record: peptidyl-tRNA hydrolase (suPth2) variant Q22A, E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys)
peptidyl-tRNA hydrolase (suPth2)variant Q54A	Sulfolobus solfataricus	E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys)	0.27	2.9 × 10⁻⁶	9.3 × 10⁴ calc.	pH 7.5, 50 °C	Open record: peptidyl-tRNA hydrolase (suPth2) variant Q54A, E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys)
peptidyl-tRNA hydrolase (suPth2)variant T90A	Sulfolobus solfataricus	E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys)	0.02	7.2 × 10⁻⁶	2.8 × 10³ calc.	pH 7.5, 50 °C	Open record: peptidyl-tRNA hydrolase (suPth2) variant T90A, E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys)
peptidyl-tRNA hydrolase (suPth2)variant T98A	Sulfolobus solfataricus	E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys)	0.06	9 × 10⁻⁷	6.7 × 10⁴ calc.	pH 7.5, 50 °C	Open record: peptidyl-tRNA hydrolase (suPth2) variant T98A, E. coli diacetyl-L-lysyl-tRNALys (N-acetylated L-lysyl-tRNALys)