Values CatLog read from this paper
Importance of lysine-286 at the NADP site of glutamate dehydrogenase from Salmonella typhimurium
PMID 1510967doi:10.1021/bi00149a010Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 49 records (71 values) from this paper: NADP+-glutamate dehydrogenase from Salmonella typhimurium.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| NADP+-glutamate dehydrogenase | Salmonella typhimurium | ammonium chloride | — | 3.6 | — | pH 7.87, 25 °C | Open record: NADP+-glutamate dehydrogenase, ammonium chloride |
| NADP+-glutamate dehydrogenase | Salmonella typhimurium | ammonium chloride | — | 9.4 | — | pH 6.9, 25 °C | Open record: NADP+-glutamate dehydrogenase, ammonium chloride |
| NADP+-glutamate dehydrogenase | Salmonella typhimurium | NADH | 20.3 | 2 | 10.3 | pH 8, 25 °C | Open record: NADP+-glutamate dehydrogenase, NADH |
| NADP+-glutamate dehydrogenase | Salmonella typhimurium | α-ketoglutarate | — | 0.27 | — | pH 7.87, 25 °C | Open record: NADP+-glutamate dehydrogenase, α-ketoglutarate |
| NADP+-glutamate dehydrogenase | Salmonella typhimurium | α-ketoglutarate | — | 0.049 | — | pH 6.9, 25 °C | Open record: NADP+-glutamate dehydrogenase, α-ketoglutarate |
| NADP+-glutamate dehydrogenase | Salmonella typhimurium | β-nicotinamide adenine dinucleotide phosphate, reduced formkcat(av) = average of kcat(I) and kcat(II) | 190 | 0.0098 | 2 × 10⁴ | pH 7.87, 25 °C | Open record: NADP+-glutamate dehydrogenase, β-nicotinamide adenine dinucleotide phosphate, reduced form |
| NADP+-glutamate dehydrogenase | Salmonella typhimurium | β-nicotinamide adenine dinucleotide phosphate, reduced formcorrected to saturating alpha-ketoglutarate | 167 | — | — | pH 7.87, 25 °C | Open record: NADP+-glutamate dehydrogenase, β-nicotinamide adenine dinucleotide phosphate, reduced form |
| NADP+-glutamate dehydrogenase | Salmonella typhimurium | β-nicotinamide adenine dinucleotide phosphate, reduced form2 mM alpha-ketoglutarate; 2000 µM NADPH | 213 | — | — | pH 8, 25 °C | Open record: NADP+-glutamate dehydrogenase, β-nicotinamide adenine dinucleotide phosphate, reduced form |
| NADP+-glutamate dehydrogenase | Salmonella typhimurium | β-nicotinamide adenine dinucleotide phosphate, reduced formnot to saturating alpha-ketoglutarate; alpha-ketoglutarate | 188 | — | 2 × 10⁴ | pH 8, 25 °C | Open record: NADP+-glutamate dehydrogenase, β-nicotinamide adenine dinucleotide phosphate, reduced form |
| NADP+-glutamate dehydrogenase | Salmonella typhimurium | β-nicotinamide adenine dinucleotide phosphate, reduced form | — | 0.0065 | — | pH 6.72, 25 °C | Open record: NADP+-glutamate dehydrogenase, β-nicotinamide adenine dinucleotide phosphate, reduced form |
| NADP+-glutamate dehydrogenase | Salmonella typhimurium | β-nicotinamide adenine dinucleotide phosphate, reduced form | — | 0.0067 | — | pH 7.4, 25 °C | Open record: NADP+-glutamate dehydrogenase, β-nicotinamide adenine dinucleotide phosphate, reduced form |
| NADP+-glutamate dehydrogenase | Salmonella typhimurium | β-nicotinamide adenine dinucleotide phosphate, reduced form | — | 0.0108 | — | pH 8.1, 25 °C | Open record: NADP+-glutamate dehydrogenase, β-nicotinamide adenine dinucleotide phosphate, reduced form |
| NADP+-glutamate dehydrogenasevariant K278Q | Salmonella typhimurium | ammonium chloride | — | 2.38 | — | pH 7.87, 25 °C | Open record: NADP+-glutamate dehydrogenase variant K278Q, ammonium chloride |
| NADP+-glutamate dehydrogenasevariant K278Q | Salmonella typhimurium | α-ketoglutarate | — | 0.456 | — | pH 7.87, 25 °C | Open record: NADP+-glutamate dehydrogenase variant K278Q, α-ketoglutarate |
| NADP+-glutamate dehydrogenasevariant K278Q | Salmonella typhimurium | β-nicotinamide adenine dinucleotide phosphate, reduced form | 175 | 0.0103 | 1.7 × 10⁴ | pH 7.87, 25 °C | Open record: NADP+-glutamate dehydrogenase variant K278Q, β-nicotinamide adenine dinucleotide phosphate, reduced form |
| NADP+-glutamate dehydrogenasevariant K286E | Salmonella typhimurium | ammonium chloride | — | 5.5 | — | pH 7.87, 25 °C | Open record: NADP+-glutamate dehydrogenase variant K286E, ammonium chloride |
| NADP+-glutamate dehydrogenasevariant K286E | Salmonella typhimurium | NADH | 15.8 | 2.1 | 7.7 | pH 8, 25 °C | Open record: NADP+-glutamate dehydrogenase variant K286E, NADH |
| NADP+-glutamate dehydrogenasevariant K286E | Salmonella typhimurium | α-ketoglutarate | — | 1 | — | pH 7.87, 25 °C | Open record: NADP+-glutamate dehydrogenase variant K286E, α-ketoglutarate |
| NADP+-glutamate dehydrogenasevariant K286E | Salmonella typhimurium | β-nicotinamide adenine dinucleotide phosphate, reduced formkcat(av) = average of kcat(I) and kcat(II) | 313 | 0.28 | 1.2 × 10³ | pH 7.87, 25 °C | Open record: NADP+-glutamate dehydrogenase variant K286E, β-nicotinamide adenine dinucleotide phosphate, reduced form |
| NADP+-glutamate dehydrogenasevariant K286E | Salmonella typhimurium | β-nicotinamide adenine dinucleotide phosphate, reduced formcorrected to saturating alpha-ketoglutarate | 325 | — | — | pH 7.87, 25 °C | Open record: NADP+-glutamate dehydrogenase variant K286E, β-nicotinamide adenine dinucleotide phosphate, reduced form |
| NADP+-glutamate dehydrogenasevariant K286E | Salmonella typhimurium | β-nicotinamide adenine dinucleotide phosphate, reduced form2 mM alpha-ketoglutarate; 2000 µM NADPH | 300 | — | — | pH 8, 25 °C | Open record: NADP+-glutamate dehydrogenase variant K286E, β-nicotinamide adenine dinucleotide phosphate, reduced form |
| NADP+-glutamate dehydrogenasevariant K286E | Salmonella typhimurium | β-nicotinamide adenine dinucleotide phosphate, reduced formnot to saturating alpha-ketoglutarate; alpha-ketoglutarate | 197 | — | 700 | pH 8, 25 °C | Open record: NADP+-glutamate dehydrogenase variant K286E, β-nicotinamide adenine dinucleotide phosphate, reduced form |
| NADP+-glutamate dehydrogenasevariant K286E | Salmonella typhimurium | β-nicotinamide adenine dinucleotide phosphate, reduced form | — | 0.0205 | — | pH 5.98, 25 °C | Open record: NADP+-glutamate dehydrogenase variant K286E, β-nicotinamide adenine dinucleotide phosphate, reduced form |
| NADP+-glutamate dehydrogenasevariant K286E | Salmonella typhimurium | β-nicotinamide adenine dinucleotide phosphate, reduced form | — | 0.0226 | — | pH 6.31, 25 °C | Open record: NADP+-glutamate dehydrogenase variant K286E, β-nicotinamide adenine dinucleotide phosphate, reduced form |
| NADP+-glutamate dehydrogenasevariant K286E | Salmonella typhimurium | β-nicotinamide adenine dinucleotide phosphate, reduced form | — | 0.0275 | — | pH 6.69, 25 °C | Open record: NADP+-glutamate dehydrogenase variant K286E, β-nicotinamide adenine dinucleotide phosphate, reduced form |
| NADP+-glutamate dehydrogenasevariant K286E | Salmonella typhimurium | β-nicotinamide adenine dinucleotide phosphate, reduced form | — | 0.0859 | — | pH 7.07, 25 °C | Open record: NADP+-glutamate dehydrogenase variant K286E, β-nicotinamide adenine dinucleotide phosphate, reduced form |
| NADP+-glutamate dehydrogenasevariant K286E | Salmonella typhimurium | β-nicotinamide adenine dinucleotide phosphate, reduced form | — | 0.171 | — | pH 7.38, 25 °C | Open record: NADP+-glutamate dehydrogenase variant K286E, β-nicotinamide adenine dinucleotide phosphate, reduced form |
| NADP+-glutamate dehydrogenasevariant K286E | Salmonella typhimurium | β-nicotinamide adenine dinucleotide phosphate, reduced form | — | 0.289 | — | pH 7.58, 25 °C | Open record: NADP+-glutamate dehydrogenase variant K286E, β-nicotinamide adenine dinucleotide phosphate, reduced form |
| NADP+-glutamate dehydrogenasevariant K286E | Salmonella typhimurium | β-nicotinamide adenine dinucleotide phosphate, reduced form | — | 0.85 | — | pH 8.08, 25 °C | Open record: NADP+-glutamate dehydrogenase variant K286E, β-nicotinamide adenine dinucleotide phosphate, reduced form |
| NADP+-glutamate dehydrogenasevariant K286Q | Salmonella typhimurium | ammonium chloride | — | 8.6 | — | pH 7.87, 25 °C | Open record: NADP+-glutamate dehydrogenase variant K286Q, ammonium chloride |
| NADP+-glutamate dehydrogenasevariant K286Q | Salmonella typhimurium | NADH | 37.5 | 2.1 | 18.3 | pH 8, 25 °C | Open record: NADP+-glutamate dehydrogenase variant K286Q, NADH |
| NADP+-glutamate dehydrogenasevariant K286Q | Salmonella typhimurium | α-ketoglutarate | — | 0.69 | — | pH 7.87, 25 °C | Open record: NADP+-glutamate dehydrogenase variant K286Q, α-ketoglutarate |
| NADP+-glutamate dehydrogenasevariant K286Q | Salmonella typhimurium | β-nicotinamide adenine dinucleotide phosphate, reduced formkcat(av) = average of kcat(I) and kcat(II) | 327 | 0.066 | 5 × 10³ | pH 7.87, 25 °C | Open record: NADP+-glutamate dehydrogenase variant K286Q, β-nicotinamide adenine dinucleotide phosphate, reduced form |
| NADP+-glutamate dehydrogenasevariant K286Q | Salmonella typhimurium | β-nicotinamide adenine dinucleotide phosphate, reduced formcorrected to saturating alpha-ketoglutarate | 343 | — | — | pH 7.87, 25 °C | Open record: NADP+-glutamate dehydrogenase variant K286Q, β-nicotinamide adenine dinucleotide phosphate, reduced form |
| NADP+-glutamate dehydrogenasevariant K286Q | Salmonella typhimurium | β-nicotinamide adenine dinucleotide phosphate, reduced form2 mM alpha-ketoglutarate; 2000 µM NADPH | 308 | — | — | pH 8, 25 °C | Open record: NADP+-glutamate dehydrogenase variant K286Q, β-nicotinamide adenine dinucleotide phosphate, reduced form |
| NADP+-glutamate dehydrogenasevariant K286Q | Salmonella typhimurium | β-nicotinamide adenine dinucleotide phosphate, reduced formnot to saturating alpha-ketoglutarate; alpha-ketoglutarate | 230 | — | 3.5 × 10³ | pH 8, 25 °C | Open record: NADP+-glutamate dehydrogenase variant K286Q, β-nicotinamide adenine dinucleotide phosphate, reduced form |
| NADP+-glutamate dehydrogenasevariant K286Q | Salmonella typhimurium | β-nicotinamide adenine dinucleotide phosphate, reduced form | — | 0.0125 | — | pH 6.3, 25 °C | Open record: NADP+-glutamate dehydrogenase variant K286Q, β-nicotinamide adenine dinucleotide phosphate, reduced form |
| NADP+-glutamate dehydrogenasevariant K286Q | Salmonella typhimurium | β-nicotinamide adenine dinucleotide phosphate, reduced form | — | 0.0194 | — | pH 6.69, 25 °C | Open record: NADP+-glutamate dehydrogenase variant K286Q, β-nicotinamide adenine dinucleotide phosphate, reduced form |
| NADP+-glutamate dehydrogenasevariant K286Q | Salmonella typhimurium | β-nicotinamide adenine dinucleotide phosphate, reduced form | — | 0.0193 | — | pH 7.02, 25 °C | Open record: NADP+-glutamate dehydrogenase variant K286Q, β-nicotinamide adenine dinucleotide phosphate, reduced form |
| NADP+-glutamate dehydrogenasevariant K286Q | Salmonella typhimurium | β-nicotinamide adenine dinucleotide phosphate, reduced form | — | 0.0182 | — | pH 7.38, 25 °C | Open record: NADP+-glutamate dehydrogenase variant K286Q, β-nicotinamide adenine dinucleotide phosphate, reduced form |
| NADP+-glutamate dehydrogenasevariant K286Q | Salmonella typhimurium | β-nicotinamide adenine dinucleotide phosphate, reduced form | — | 0.0393 | — | pH 7.57, 25 °C | Open record: NADP+-glutamate dehydrogenase variant K286Q, β-nicotinamide adenine dinucleotide phosphate, reduced form |
| NADP+-glutamate dehydrogenasevariant K286Q | Salmonella typhimurium | β-nicotinamide adenine dinucleotide phosphate, reduced form | — | 0.0801 | — | pH 8.08, 25 °C | Open record: NADP+-glutamate dehydrogenase variant K286Q, β-nicotinamide adenine dinucleotide phosphate, reduced form |
| NADP+-glutamate dehydrogenasevariant K286R | Salmonella typhimurium | ammonium chloride | — | 3.7 | — | pH 7.87, 25 °C | Open record: NADP+-glutamate dehydrogenase variant K286R, ammonium chloride |
| NADP+-glutamate dehydrogenasevariant K286R | Salmonella typhimurium | NADH | 49.5 | 1.8 | 26.7 | pH 8, 25 °C | Open record: NADP+-glutamate dehydrogenase variant K286R, NADH |
| NADP+-glutamate dehydrogenasevariant K286R | Salmonella typhimurium | α-ketoglutarate | — | 0.68 | — | pH 7.87, 25 °C | Open record: NADP+-glutamate dehydrogenase variant K286R, α-ketoglutarate |
| NADP+-glutamate dehydrogenasevariant K286R | Salmonella typhimurium | β-nicotinamide adenine dinucleotide phosphate, reduced formkcat(av) = average of kcat(I) and kcat(II) | 475 | 0.03 | 1.58 × 10⁴ | pH 7.87, 25 °C | Open record: NADP+-glutamate dehydrogenase variant K286R, β-nicotinamide adenine dinucleotide phosphate, reduced form |
| NADP+-glutamate dehydrogenasevariant K286R | Salmonella typhimurium | β-nicotinamide adenine dinucleotide phosphate, reduced formcorrected to saturating alpha-ketoglutarate | 547 | — | — | pH 7.87, 25 °C | Open record: NADP+-glutamate dehydrogenase variant K286R, β-nicotinamide adenine dinucleotide phosphate, reduced form |
| NADP+-glutamate dehydrogenasevariant K286R | Salmonella typhimurium | β-nicotinamide adenine dinucleotide phosphate, reduced form2 mM alpha-ketoglutarate; 2000 µM NADPH | 405 | — | — | pH 8, 25 °C | Open record: NADP+-glutamate dehydrogenase variant K286R, β-nicotinamide adenine dinucleotide phosphate, reduced form |
| NADP+-glutamate dehydrogenasevariant K286R | Salmonella typhimurium | β-nicotinamide adenine dinucleotide phosphate, reduced formnot to saturating alpha-ketoglutarate; alpha-ketoglutarate | 302 | — | 1.02 × 10⁴ | pH 8, 25 °C | Open record: NADP+-glutamate dehydrogenase variant K286R, β-nicotinamide adenine dinucleotide phosphate, reduced form |