Values CatLog read from this paper
Cross-talk between thiamin diphosphate binding and phosphorylation loop conformation in human branched-chain alpha-keto acid decarboxylase/dehydrogenase
PMID 15166214doi:10.1074/jbc.m403611200Open in Browse
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CatLog read 22 records (66 values) from this paper: branched-chain alpha-keto acid decarboxylase/dehydrogenase (E1b, alpha2beta2 heterotetramer), branched-chain alpha-keto acid dehydrogenase (BCKD) complex reconstituted with E1b, lipoylated E2b and E3 from Homo sapiens.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| branched-chain alpha-keto acid decarboxylase/dehydrogenase (E1b, alpha2beta2 heterotetramer) | Homo sapiens | alpha-ketoisovalerate (3-methyl-2-oxobutanoate) | 0.195 | 0.0502 | 3.8 | 30 °C | Open record: branched-chain alpha-keto acid decarboxylase/dehydrogenase (E1b, alpha2beta2 heterotetramer), alpha-ketoisovalerate (3-methyl-2-oxobutanoate) |
| branched-chain alpha-keto acid decarboxylase/dehydrogenase (E1b, alpha2beta2 heterotetramer) | Homo sapiens | thiamin diphosphate | 0.197 | 0.0066 | 29.8 | 30 °C | Open record: branched-chain alpha-keto acid decarboxylase/dehydrogenase (E1b, alpha2beta2 heterotetramer), thiamin diphosphate |
| branched-chain alpha-keto acid decarboxylase/dehydrogenase (E1b, alpha2beta2 heterotetramer)variant D295A-α | Homo sapiens | alpha-ketoisovalerate (3-methyl-2-oxobutanoate) | 0.485 | 0.0505 | 9.7 | 30 °C | Open record: branched-chain alpha-keto acid decarboxylase/dehydrogenase (E1b, alpha2beta2 heterotetramer) variant D295A-α, alpha-ketoisovalerate (3-methyl-2-oxobutanoate) |
| branched-chain alpha-keto acid decarboxylase/dehydrogenase (E1b, alpha2beta2 heterotetramer)variant D295A-α | Homo sapiens | thiamin diphosphate | 0.423 | 0.0166 | 25.5 | 30 °C | Open record: branched-chain alpha-keto acid decarboxylase/dehydrogenase (E1b, alpha2beta2 heterotetramer) variant D295A-α, thiamin diphosphate |
| branched-chain alpha-keto acid decarboxylase/dehydrogenase (E1b, alpha2beta2 heterotetramer)variant R287A-α | Homo sapiens | alpha-ketoisovalerate (3-methyl-2-oxobutanoate) | 0.38 | 2.33 | 0.17 | 30 °C | Open record: branched-chain alpha-keto acid decarboxylase/dehydrogenase (E1b, alpha2beta2 heterotetramer) variant R287A-α, alpha-ketoisovalerate (3-methyl-2-oxobutanoate) |
| branched-chain alpha-keto acid decarboxylase/dehydrogenase (E1b, alpha2beta2 heterotetramer)variant R287A-α | Homo sapiens | thiamin diphosphate | 0.392 | 0.0236 | 16.7 | 30 °C | Open record: branched-chain alpha-keto acid decarboxylase/dehydrogenase (E1b, alpha2beta2 heterotetramer) variant R287A-α, thiamin diphosphate |
| branched-chain alpha-keto acid decarboxylase/dehydrogenase (E1b, alpha2beta2 heterotetramer)variant R301A-α | Homo sapiens | alpha-ketoisovalerate (3-methyl-2-oxobutanoate) | 0.413 | 0.225 | 1.8 | 30 °C | Open record: branched-chain alpha-keto acid decarboxylase/dehydrogenase (E1b, alpha2beta2 heterotetramer) variant R301A-α, alpha-ketoisovalerate (3-methyl-2-oxobutanoate) |
| branched-chain alpha-keto acid decarboxylase/dehydrogenase (E1b, alpha2beta2 heterotetramer)variant R301A-α | Homo sapiens | thiamin diphosphate | 0.42 | 0.0289 | 14.5 | 30 °C | Open record: branched-chain alpha-keto acid decarboxylase/dehydrogenase (E1b, alpha2beta2 heterotetramer) variant R301A-α, thiamin diphosphate |
| branched-chain alpha-keto acid decarboxylase/dehydrogenase (E1b, alpha2beta2 heterotetramer)variant Y300A-α | Homo sapiens | alpha-ketoisovalerate (3-methyl-2-oxobutanoate) | 0.355 | 0.337 | 1 | 30 °C | Open record: branched-chain alpha-keto acid decarboxylase/dehydrogenase (E1b, alpha2beta2 heterotetramer) variant Y300A-α, alpha-ketoisovalerate (3-methyl-2-oxobutanoate) |
| branched-chain alpha-keto acid decarboxylase/dehydrogenase (E1b, alpha2beta2 heterotetramer)variant Y300A-α | Homo sapiens | thiamin diphosphate | 0.308 | 0.0401 | 7.7 | 30 °C | Open record: branched-chain alpha-keto acid decarboxylase/dehydrogenase (E1b, alpha2beta2 heterotetramer) variant Y300A-α, thiamin diphosphate |
| branched-chain alpha-keto acid decarboxylase/dehydrogenase (E1b, alpha2beta2 heterotetramer)variant Y300F-α | Homo sapiens | alpha-ketoisovalerate (3-methyl-2-oxobutanoate) | 0.568 | 0.252 | 2.3 | 30 °C | Open record: branched-chain alpha-keto acid decarboxylase/dehydrogenase (E1b, alpha2beta2 heterotetramer) variant Y300F-α, alpha-ketoisovalerate (3-methyl-2-oxobutanoate) |
| branched-chain alpha-keto acid decarboxylase/dehydrogenase (E1b, alpha2beta2 heterotetramer)variant Y300F-α | Homo sapiens | thiamin diphosphate | 0.53 | 0.0286 | 18.5 | 30 °C | Open record: branched-chain alpha-keto acid decarboxylase/dehydrogenase (E1b, alpha2beta2 heterotetramer) variant Y300F-α, thiamin diphosphate |
| branched-chain alpha-keto acid dehydrogenase (BCKD) complex reconstituted with E1b, lipoylated E2b and E3 | Homo sapiens | alpha-ketoisovalerate (3-methyl-2-oxobutanoate) | 3.13 | 0.0503 | 62.3 | Open record: branched-chain alpha-keto acid dehydrogenase (BCKD) complex reconstituted with E1b, lipoylated E2b and E3, alpha-ketoisovalerate (3-methyl-2-oxobutanoate) | |
| branched-chain alpha-keto acid dehydrogenase (BCKD) complex reconstituted with E1b, lipoylated E2b and E3 | Homo sapiens | thiamin diphosphate | 3.17 | 6.3 × 10⁻⁴ | 5.03 × 10³ | Open record: branched-chain alpha-keto acid dehydrogenase (BCKD) complex reconstituted with E1b, lipoylated E2b and E3, thiamin diphosphate | |
| branched-chain alpha-keto acid dehydrogenase (BCKD) complex reconstituted with E1b, lipoylated E2b and E3variant D295A-α | Homo sapiens | alpha-ketoisovalerate (3-methyl-2-oxobutanoate) | 0.772 | 0.0509 | 15.2 | Open record: branched-chain alpha-keto acid dehydrogenase (BCKD) complex reconstituted with E1b, lipoylated E2b and E3 variant D295A-α, alpha-ketoisovalerate (3-methyl-2-oxobutanoate) | |
| branched-chain alpha-keto acid dehydrogenase (BCKD) complex reconstituted with E1b, lipoylated E2b and E3variant D295A-α | Homo sapiens | thiamin diphosphate | 0.768 | 0.0151 | 52 | Open record: branched-chain alpha-keto acid dehydrogenase (BCKD) complex reconstituted with E1b, lipoylated E2b and E3 variant D295A-α, thiamin diphosphate | |
| branched-chain alpha-keto acid dehydrogenase (BCKD) complex reconstituted with E1b, lipoylated E2b and E3variant R301A-α | Homo sapiens | alpha-ketoisovalerate (3-methyl-2-oxobutanoate) | 0.823 | 0.255 | 3.2 | Open record: branched-chain alpha-keto acid dehydrogenase (BCKD) complex reconstituted with E1b, lipoylated E2b and E3 variant R301A-α, alpha-ketoisovalerate (3-methyl-2-oxobutanoate) | |
| branched-chain alpha-keto acid dehydrogenase (BCKD) complex reconstituted with E1b, lipoylated E2b and E3variant R301A-α | Homo sapiens | thiamin diphosphate | 0.845 | 0.0262 | 32 | Open record: branched-chain alpha-keto acid dehydrogenase (BCKD) complex reconstituted with E1b, lipoylated E2b and E3 variant R301A-α, thiamin diphosphate | |
| branched-chain alpha-keto acid dehydrogenase (BCKD) complex reconstituted with E1b, lipoylated E2b and E3variant Y300A-α | Homo sapiens | alpha-ketoisovalerate (3-methyl-2-oxobutanoate) | 0.447 | 0.14 | 3.2 | Open record: branched-chain alpha-keto acid dehydrogenase (BCKD) complex reconstituted with E1b, lipoylated E2b and E3 variant Y300A-α, alpha-ketoisovalerate (3-methyl-2-oxobutanoate) | |
| branched-chain alpha-keto acid dehydrogenase (BCKD) complex reconstituted with E1b, lipoylated E2b and E3variant Y300A-α | Homo sapiens | thiamin diphosphate | 0.462 | 0.0264 | 17 | Open record: branched-chain alpha-keto acid dehydrogenase (BCKD) complex reconstituted with E1b, lipoylated E2b and E3 variant Y300A-α, thiamin diphosphate | |
| branched-chain alpha-keto acid dehydrogenase (BCKD) complex reconstituted with E1b, lipoylated E2b and E3variant Y300F-α | Homo sapiens | alpha-ketoisovalerate (3-methyl-2-oxobutanoate) | 1.5 | 0.39 | 3.8 | Open record: branched-chain alpha-keto acid dehydrogenase (BCKD) complex reconstituted with E1b, lipoylated E2b and E3 variant Y300F-α, alpha-ketoisovalerate (3-methyl-2-oxobutanoate) | |
| branched-chain alpha-keto acid dehydrogenase (BCKD) complex reconstituted with E1b, lipoylated E2b and E3variant Y300F-α | Homo sapiens | thiamin diphosphate | 1.427 | 0.0185 | 77 | Open record: branched-chain alpha-keto acid dehydrogenase (BCKD) complex reconstituted with E1b, lipoylated E2b and E3 variant Y300F-α, thiamin diphosphate |