CatLogenzyme kinetics catalog

EC 1.2.4.4: 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring)

OxidoreductasesOpen in Browse

CatLog read 15 records for EC 1.2.4.4 from 2 papers, for 1 organism. Names in these papers: branched-chain α-ketoacid decarboxylase/dehydrogenase (E1b), branched-chain alpha-keto acid decarboxylase/dehydrogenase (E1b, alpha2beta2 heterotetramer), branched-chain α-ketoacid dehydrogenase complex (BCKDC) reconstituted with E1b, lipoylated E2b and E3.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 47 records for EC 1.2.4.4 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 1.2.4.4 in Browse.

Papers

PaperOrganismsRecords
Molecular mechanism for regulation of the human mitochondrial branched-chain alpha-ketoacid dehydrogenase complex by phosphorylationHomo sapiens10
Cross-talk between thiamin diphosphate binding and phosphorylation loop conformation in human branched-chain alpha-keto acid decarboxylase/dehydrogenaseHomo sapiens5

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
branched-chain alpha-keto acid decarboxylase/dehydrogenase (E1b, alpha2beta2 heterotetramer)Homo sapiensalpha-ketoisovalerate (3-methyl-2-oxobutanoate)0.1950.05023.830 °CPMID 15166214Open
branched-chain alpha-keto acid decarboxylase/dehydrogenase (E1b, alpha2beta2 heterotetramer)variant D295A-αHomo sapiensthiamin diphosphate0.4230.016625.530 °CPMID 15166214Open
branched-chain alpha-keto acid decarboxylase/dehydrogenase (E1b, alpha2beta2 heterotetramer)variant R287A-αHomo sapiensalpha-ketoisovalerate (3-methyl-2-oxobutanoate)0.382.330.1730 °CPMID 15166214Open
branched-chain alpha-keto acid decarboxylase/dehydrogenase (E1b, alpha2beta2 heterotetramer)variant R287A-αHomo sapiensthiamin diphosphate0.3920.023616.730 °CPMID 15166214Open
branched-chain alpha-keto acid decarboxylase/dehydrogenase (E1b, alpha2beta2 heterotetramer)variant Y300A-αHomo sapiensalpha-ketoisovalerate (3-methyl-2-oxobutanoate)0.3550.337130 °CPMID 15166214Open
branched-chain α-ketoacid decarboxylase/dehydrogenase (E1b)Homo sapiensα-ketoisovalerate0.20.054PMID 15576032Open
branched-chain α-ketoacid decarboxylase/dehydrogenase (E1b)variant S292-α-PO3 (S302A background, Ser292-α phosphorylated)Homo sapiensα-ketoisovalerate0.150.9830.15PMID 15576032Open
branched-chain α-ketoacid decarboxylase/dehydrogenase (E1b)variant S292D/S302AHomo sapiensα-ketoisovalerate1.654.8960.33PMID 15576032Open
branched-chain α-ketoacid decarboxylase/dehydrogenase (E1b)variant S292E/S302AHomo sapiensα-ketoisovalerate0.1280.5930.22PMID 15576032Open
branched-chain α-ketoacid decarboxylase/dehydrogenase (E1b)variant S292N/S302AHomo sapiensα-ketoisovalerate2.770.22412.3PMID 15576032Open
branched-chain α-ketoacid decarboxylase/dehydrogenase (E1b)variant S302AHomo sapiensα-ketoisovalerate0.620.02228PMID 15576032Open
branched-chain α-ketoacid dehydrogenase complex (BCKDC) reconstituted with E1b, lipoylated E2b and E3Homo sapiensα-ketoisovalerate3.30.04867PMID 15576032Open
branched-chain α-ketoacid dehydrogenase complex (BCKDC) reconstituted with E1b, lipoylated E2b and E3variant S292D/S302AHomo sapiensα-ketoisovalerate0.584.3810.132PMID 15576032Open
branched-chain α-ketoacid dehydrogenase complex (BCKDC) reconstituted with E1b, lipoylated E2b and E3variant S292N/S302AHomo sapiensα-ketoisovalerate3.920.19320.2PMID 15576032Open
branched-chain α-ketoacid dehydrogenase complex (BCKDC) reconstituted with E1b, lipoylated E2b and E3variant S302AHomo sapiensα-ketoisovalerate2.180.02877PMID 15576032Open