CatLogenzyme kinetics catalog

Values CatLog read from this paper

The HD domain of the Escherichia coli tRNA nucleotidyltransferase has 2',3'-cyclic phosphodiesterase, 2'-nucleotidase, and phosphatase activities

PMID 15210699doi:10.1074/jbc.m405120200Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 16 records (36 values) from this paper: tRNA nucleotidyltransferase (CCA-adding enzyme, tRNA-NT), HD domain phosphohydrolase activity from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.7.7.25

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
tRNA nucleotidyltransferase (CCA-adding enzyme, tRNA-NT), HD domain phosphohydrolase activityEscherichia coliadenosine 2',3'-cyclic monophosphate0.1 mM Ni2+; Table I; substrate 0.1-10 mM; nonlinear fit7.630.491.98pH 7, 37 °COpen
tRNA nucleotidyltransferase (CCA-adding enzyme, tRNA-NT), HD domain phosphohydrolase activityEscherichia coliadenosine 2',3'-cyclic monophosphate0.28 µg enzyme; no tRNA added; Table II—0.49—pH 7, 37 °COpen
tRNA nucleotidyltransferase (CCA-adding enzyme, tRNA-NT), HD domain phosphohydrolase activityEscherichia coliadenosine 2',3'-cyclic monophosphate0.28 µg enzyme; apparent Km; Table II—0.89—pH 7, 37 °COpen
tRNA nucleotidyltransferase (CCA-adding enzyme, tRNA-NT), HD domain phosphohydrolase activityEscherichia coliadenosine 2',3'-cyclic monophosphate0.28 µg enzyme; apparent Km; Table II—6.79—pH 7, 37 °COpen
tRNA nucleotidyltransferase (CCA-adding enzyme, tRNA-NT), HD domain phosphohydrolase activityEscherichia coliadenosine 2'-monophosphate0.1 mM Ni2+; Pi released measured with Malachite Green3.090.764.07pH 7.5, 37 °COpen
tRNA nucleotidyltransferase (CCA-adding enzyme, tRNA-NT), HD domain phosphohydrolase activityEscherichia coliadenosine 2'-monophosphate0.28 µg enzyme; no tRNA added; Table II—0.69—pH 7.5, 37 °COpen
tRNA nucleotidyltransferase (CCA-adding enzyme, tRNA-NT), HD domain phosphohydrolase activityEscherichia coliadenosine 2'-monophosphate0.28 µg enzyme; apparent Km; Table II—2.06—pH 7.5, 37 °COpen
tRNA nucleotidyltransferase (CCA-adding enzyme, tRNA-NT), HD domain phosphohydrolase activityEscherichia coliadenosine 2'-monophosphate0.28 µg enzyme; apparent Km; Table II—4.21—pH 7.5, 37 °COpen
tRNA nucleotidyltransferase (CCA-adding enzyme, tRNA-NT), HD domain phosphohydrolase activityEscherichia coliadenosine 5'-diphosphate1.240.196.53pH 7.5, 37 °COpen
tRNA nucleotidyltransferase (CCA-adding enzyme, tRNA-NT), HD domain phosphohydrolase activityEscherichia coliadenosine 5'-triphosphate3.780.1821pH 7.5, 37 °COpen
tRNA nucleotidyltransferase (CCA-adding enzyme, tRNA-NT), HD domain phosphohydrolase activityEscherichia colicytidine 5'-diphosphate4.830.539.11pH 7.5, 37 °COpen
tRNA nucleotidyltransferase (CCA-adding enzyme, tRNA-NT), HD domain phosphohydrolase activityEscherichia colicytidine 5'-triphosphate3.360.1325.9pH 7.5, 37 °COpen
tRNA nucleotidyltransferase (CCA-adding enzyme, tRNA-NT), HD domain phosphohydrolase activityEscherichia coliguanosine 2',3'-cyclic monophosphate1.971.61.23pH 7, 37 °COpen
tRNA nucleotidyltransferase (CCA-adding enzyme, tRNA-NT), HD domain phosphohydrolase activityEscherichia colinicotinamide adenine dinucleotide phosphate14.90.1599.3pH 7.5, 37 °COpen
tRNA nucleotidyltransferase (CCA-adding enzyme, tRNA-NT), HD domain phosphohydrolase activityEscherichia colip-nitrophenyl phosphate10.36.21.66pH 7.5, 37 °COpen
tRNA nucleotidyltransferase (CCA-adding enzyme, tRNA-NT), HD domain phosphohydrolase activityEscherichia colipyrophosphate2.510.125.1pH 7.5, 37 °COpen