CatLogenzyme kinetics catalog

EC 2.7.7.25: tRNA adenylyltransferase

TransferasesOpen in Browse

CatLog read 16 records for EC 2.7.7.25 from 1 paper, for 1 organism. Names in these papers: tRNA nucleotidyltransferase (CCA-adding enzyme, tRNA-NT), HD domain phosphohydrolase activity.

EC 2.7.7.25 is no longer current; BRENDA: “transferred to EC 2.7.7.72”.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

Papers

PaperOrganismsRecords
The HD domain of the Escherichia coli tRNA nucleotidyltransferase has 2',3'-cyclic phosphodiesterase, 2'-nucleotidase, and phosphatase activitiesEscherichia coli16

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
tRNA nucleotidyltransferase (CCA-adding enzyme, tRNA-NT), HD domain phosphohydrolase activityEscherichia coliadenosine 2',3'-cyclic monophosphate0.1 mM Ni2+; Table I; substrate 0.1-10 mM; nonlinear fit7.630.491.98pH 7, 37 °CPMID 15210699Open
tRNA nucleotidyltransferase (CCA-adding enzyme, tRNA-NT), HD domain phosphohydrolase activityEscherichia coliadenosine 2',3'-cyclic monophosphate0.28 µg enzyme; no tRNA added; Table II—0.49—pH 7, 37 °CPMID 15210699Open
tRNA nucleotidyltransferase (CCA-adding enzyme, tRNA-NT), HD domain phosphohydrolase activityEscherichia coliadenosine 2',3'-cyclic monophosphate0.28 µg enzyme; apparent Km; Table II—0.89—pH 7, 37 °CPMID 15210699Open
tRNA nucleotidyltransferase (CCA-adding enzyme, tRNA-NT), HD domain phosphohydrolase activityEscherichia coliadenosine 2',3'-cyclic monophosphate0.28 µg enzyme; apparent Km; Table II—6.79—pH 7, 37 °CPMID 15210699Open
tRNA nucleotidyltransferase (CCA-adding enzyme, tRNA-NT), HD domain phosphohydrolase activityEscherichia coliadenosine 2'-monophosphate0.1 mM Ni2+; Pi released measured with Malachite Green3.090.764.07pH 7.5, 37 °CPMID 15210699Open
tRNA nucleotidyltransferase (CCA-adding enzyme, tRNA-NT), HD domain phosphohydrolase activityEscherichia coliadenosine 2'-monophosphate0.28 µg enzyme; no tRNA added; Table II—0.69—pH 7.5, 37 °CPMID 15210699Open
tRNA nucleotidyltransferase (CCA-adding enzyme, tRNA-NT), HD domain phosphohydrolase activityEscherichia coliadenosine 2'-monophosphate0.28 µg enzyme; apparent Km; Table II—2.06—pH 7.5, 37 °CPMID 15210699Open
tRNA nucleotidyltransferase (CCA-adding enzyme, tRNA-NT), HD domain phosphohydrolase activityEscherichia coliadenosine 2'-monophosphate0.28 µg enzyme; apparent Km; Table II—4.21—pH 7.5, 37 °CPMID 15210699Open
tRNA nucleotidyltransferase (CCA-adding enzyme, tRNA-NT), HD domain phosphohydrolase activityEscherichia coliadenosine 5'-diphosphate1.240.196.53pH 7.5, 37 °CPMID 15210699Open
tRNA nucleotidyltransferase (CCA-adding enzyme, tRNA-NT), HD domain phosphohydrolase activityEscherichia coliadenosine 5'-triphosphate3.780.1821pH 7.5, 37 °CPMID 15210699Open
tRNA nucleotidyltransferase (CCA-adding enzyme, tRNA-NT), HD domain phosphohydrolase activityEscherichia colicytidine 5'-diphosphate4.830.539.11pH 7.5, 37 °CPMID 15210699Open
tRNA nucleotidyltransferase (CCA-adding enzyme, tRNA-NT), HD domain phosphohydrolase activityEscherichia colicytidine 5'-triphosphate3.360.1325.9pH 7.5, 37 °CPMID 15210699Open
tRNA nucleotidyltransferase (CCA-adding enzyme, tRNA-NT), HD domain phosphohydrolase activityEscherichia coliguanosine 2',3'-cyclic monophosphate1.971.61.23pH 7, 37 °CPMID 15210699Open
tRNA nucleotidyltransferase (CCA-adding enzyme, tRNA-NT), HD domain phosphohydrolase activityEscherichia colinicotinamide adenine dinucleotide phosphate14.90.1599.3pH 7.5, 37 °CPMID 15210699Open
tRNA nucleotidyltransferase (CCA-adding enzyme, tRNA-NT), HD domain phosphohydrolase activityEscherichia colip-nitrophenyl phosphate10.36.21.66pH 7.5, 37 °CPMID 15210699Open
tRNA nucleotidyltransferase (CCA-adding enzyme, tRNA-NT), HD domain phosphohydrolase activityEscherichia colipyrophosphate2.510.125.1pH 7.5, 37 °CPMID 15210699Open