Values CatLog read from this paper
Brassica juncea 3-hydroxy-3-methylglutaryl (HMG)-CoA synthase 1: expression and characterization of recombinant wild-type and mutant enzymes
PMID 15233626doi:10.1042/bj20040721Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 11 records (20 values) from this paper: 3-hydroxy-3-methylglutaryl-CoA synthase 1 (BjHMGS1) from Brassica juncea.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 9 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 3-hydroxy-3-methylglutaryl-CoA synthase 1 (BjHMGS1) | Brassica juncea | acetyl-CoA | 0.415 | 0.043 | 9.7 calc. | pH 7.5, 30 °C | Open record: 3-hydroxy-3-methylglutaryl-CoA synthase 1 (BjHMGS1), acetyl-CoA |
| 3-hydroxy-3-methylglutaryl-CoA synthase 1 (BjHMGS1)variant C212S | Brassica juncea | acetyl-CoA | 0.068 | 0.029 | 2.4 calc. | pH 7.5, 30 °C | Open record: 3-hydroxy-3-methylglutaryl-CoA synthase 1 (BjHMGS1) variant C212S, acetyl-CoA |
| 3-hydroxy-3-methylglutaryl-CoA synthase 1 (BjHMGS1)variant H188N | Brassica juncea | acetoacetyl-CoA | — | 0.012 | — | pH 7.5, 30 °C | Open record: 3-hydroxy-3-methylglutaryl-CoA synthase 1 (BjHMGS1) variant H188N, acetoacetyl-CoA |
| 3-hydroxy-3-methylglutaryl-CoA synthase 1 (BjHMGS1)variant H188N | Brassica juncea | acetyl-CoA | 0.048 | 0.055 | 0.88 calc. | pH 7.5, 30 °C | Open record: 3-hydroxy-3-methylglutaryl-CoA synthase 1 (BjHMGS1) variant H188N, acetyl-CoA |
| 3-hydroxy-3-methylglutaryl-CoA synthase 1 (BjHMGS1)variant H188N/S359A | Brassica juncea | acetoacetyl-CoA | — | 0.0029 | — | pH 7.5, 30 °C | Open record: 3-hydroxy-3-methylglutaryl-CoA synthase 1 (BjHMGS1) variant H188N/S359A, acetoacetyl-CoA |
| 3-hydroxy-3-methylglutaryl-CoA synthase 1 (BjHMGS1)variant H188N/S359A | Brassica juncea | acetyl-CoA | 3.78 | 0.12 | 32 calc. | pH 7.5, 30 °C | Open record: 3-hydroxy-3-methylglutaryl-CoA synthase 1 (BjHMGS1) variant H188N/S359A, acetyl-CoA |
| 3-hydroxy-3-methylglutaryl-CoA synthase 1 (BjHMGS1)variant N115A | Brassica juncea | acetyl-CoA | 0.39 | 0.027 | 14 calc. | pH 7.5, 30 °C | Open record: 3-hydroxy-3-methylglutaryl-CoA synthase 1 (BjHMGS1) variant N115A, acetyl-CoA |
| 3-hydroxy-3-methylglutaryl-CoA synthase 1 (BjHMGS1)variant R157A | Brassica juncea | acetyl-CoA | 0.028 | 0.052 | 0.54 calc. | pH 7.5, 30 °C | Open record: 3-hydroxy-3-methylglutaryl-CoA synthase 1 (BjHMGS1) variant R157A, acetyl-CoA |
| 3-hydroxy-3-methylglutaryl-CoA synthase 1 (BjHMGS1)variant S356A | Brassica juncea | acetyl-CoA | 0.268 | 0.026 | 10 calc. | pH 7.5, 30 °C | Open record: 3-hydroxy-3-methylglutaryl-CoA synthase 1 (BjHMGS1) variant S356A, acetyl-CoA |
| 3-hydroxy-3-methylglutaryl-CoA synthase 1 (BjHMGS1)variant S359A | Brassica juncea | acetyl-CoA | 3.568 | 0.024 | 150 calc. | pH 7.5, 30 °C | Open record: 3-hydroxy-3-methylglutaryl-CoA synthase 1 (BjHMGS1) variant S359A, acetyl-CoA |
| 3-hydroxy-3-methylglutaryl-CoA synthase 1 (BjHMGS1)variant S89A | Brassica juncea | acetyl-CoA | 0.127 | 0.018 | 7.0 calc. | pH 7.5, 30 °C | Open record: 3-hydroxy-3-methylglutaryl-CoA synthase 1 (BjHMGS1) variant S89A, acetyl-CoA |