Values CatLog read from this paper
Expression, purification, and kinetic constants for human and Escherichia coli pyridoxal kinases
PMID 15249053doi:10.1016/j.pep.2004.04.021Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 21 records (32 values) from this paper: pyridoxal kinase 1 (ePL kinase 1), pyridoxal kinase (hPL kinase) from Escherichia coli, Homo sapiens.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 11 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| pyridoxal kinase (hPL kinase) | Homo sapiens | ATPdirect assay: PLP formation followed at 388 nm | — | 0.42 | — | pH 7.3, 37 °C | Open record: pyridoxal kinase (hPL kinase), ATP |
| pyridoxal kinase (hPL kinase) | Homo sapiens | ATPstopped with EDTA/boiling; cosubstrate PN | — | 0.21 | — | pH 7.3, 37 °C | Open record: pyridoxal kinase (hPL kinase), ATP |
| pyridoxal kinase (hPL kinase) | Homo sapiens | ATPstopped with EDTA/boiling; cosubstrate PM | — | 0.33 | — | pH 7.3, 37 °C | Open record: pyridoxal kinase (hPL kinase), ATP |
| pyridoxal kinase (hPL kinase) | Homo sapiens | pyridoxalcosubstrate MgATP | 0.75 | 0.03 | 25 calc. | pH 7.3, 37 °C | Open record: pyridoxal kinase (hPL kinase), pyridoxal |
| pyridoxal kinase (hPL kinase) | Homo sapiens | pyridoxalcosubstrate ZnATP | 0.5 | 0.35 | 1.4 calc. | pH 7.3, 37 °C | Open record: pyridoxal kinase (hPL kinase), pyridoxal |
| pyridoxal kinase (hPL kinase) | Homo sapiens | pyridoxamine | 0.33 | 0.035 | 9.5 calc. | pH 7.3, 37 °C | Open record: pyridoxal kinase (hPL kinase), pyridoxamine |
| pyridoxal kinase (hPL kinase) | Homo sapiens | pyridoxinecosubstrate MgATP | 0.33 | 0.02 | 17 calc. | pH 7.3, 37 °C | Open record: pyridoxal kinase (hPL kinase), pyridoxine |
| pyridoxal kinase (hPL kinase) | Homo sapiens | pyridoxinecosubstrate ZnATP | 0.12 | 0.015 | 7.8 calc. | pH 7.3, 37 °C | Open record: pyridoxal kinase (hPL kinase), pyridoxine |
| pyridoxal kinase 1 (ePL kinase 1) | Escherichia coli | ATPdirect assay: PLP formation followed at 388 nm | — | 0.6 | — | pH 7.3, 37 °C | Open record: pyridoxal kinase 1 (ePL kinase 1), ATP |
| pyridoxal kinase 1 (ePL kinase 1) | Escherichia coli | ATP | — | 2.1 | — | pH 6.1, 37 °C | Open record: pyridoxal kinase 1 (ePL kinase 1), ATP |
| pyridoxal kinase 1 (ePL kinase 1) | Escherichia coli | ATPstopped with EDTA/boiling; cosubstrate PN | — | 0.65 | — | pH 7.3, 37 °C | Open record: pyridoxal kinase 1 (ePL kinase 1), ATP |
| pyridoxal kinase 1 (ePL kinase 1) | Escherichia coli | ATPstopped with EDTA/boiling; cosubstrate PM | — | 0.6 | — | pH 7.3, 37 °C | Open record: pyridoxal kinase 1 (ePL kinase 1), ATP |
| pyridoxal kinase 1 (ePL kinase 1) | Escherichia coli | ATPdirect assay: PLP formation followed at 388 nm | — | 0.07 | — | pH 7.3, 37 °C | Open record: pyridoxal kinase 1 (ePL kinase 1), ATP |
| pyridoxal kinase 1 (ePL kinase 1) | Escherichia coli | ATPstopped with EDTA/boiling; cosubstrate PM | — | 0.045 | — | pH 7.3, 37 °C | Open record: pyridoxal kinase 1 (ePL kinase 1), ATP |
| pyridoxal kinase 1 (ePL kinase 1) | Escherichia coli | pyridoxalcosubstrate MgATP | 2.3 | 0.1 | 23 calc. | pH 7.3, 37 °C | Open record: pyridoxal kinase 1 (ePL kinase 1), pyridoxal |
| pyridoxal kinase 1 (ePL kinase 1) | Escherichia coli | pyridoxalcosubstrate MgATP | 2 | 0.36 | 5.6 calc. | pH 6.1, 37 °C | Open record: pyridoxal kinase 1 (ePL kinase 1), pyridoxal |
| pyridoxal kinase 1 (ePL kinase 1) | Escherichia coli | pyridoxalcosubstrate ZnATP | 2 | 0.19 | 11 calc. | pH 7.3, 37 °C | Open record: pyridoxal kinase 1 (ePL kinase 1), pyridoxal |
| pyridoxal kinase 1 (ePL kinase 1) | Escherichia coli | pyridoxalcosubstrate ZnATP | — | 0.13 | — | pH 6.1, 37 °C | Open record: pyridoxal kinase 1 (ePL kinase 1), pyridoxal |
| pyridoxal kinase 1 (ePL kinase 1) | Escherichia coli | pyridoxaminecosubstrate MgATP | 0.67 | 0.03 | 22 calc. | pH 7.3, 37 °C | Open record: pyridoxal kinase 1 (ePL kinase 1), pyridoxamine |
| pyridoxal kinase 1 (ePL kinase 1) | Escherichia coli | pyridoxaminecosubstrate ZnATP | 0.42 | 0.01 | 42 calc. | pH 7.3, 37 °C | Open record: pyridoxal kinase 1 (ePL kinase 1), pyridoxamine |
| pyridoxal kinase 1 (ePL kinase 1) | Escherichia coli | pyridoxine | 0.33 | 0.025 | 13 calc. | pH 7.3, 37 °C | Open record: pyridoxal kinase 1 (ePL kinase 1), pyridoxine |