CatLogenzyme kinetics catalog

Values CatLog read from this paper

Mitochondrial malate dehydrogenase from the thermophilic, filamentous fungus Talaromyces emersonii

PMID 15265031doi:10.1111/j.1432-1033.2004.04230.xOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 8 records (24 values) from this paper: mitochondrial malate dehydrogenase (m-MDH) from Talaromyces emersonii.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.1.1.37

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
mitochondrial malate dehydrogenase (m-MDH)Talaromyces emersoniiL-malatenative enzyme purified from T. emersonii mycelia2.9 × 10³1.252.32 × 10³pH 7.4, 30 °COpen
mitochondrial malate dehydrogenase (m-MDH)Talaromyces emersoniiL-malateexpressed in E. coli BL21-AI; Ni-NTA purified3.5 × 10³12.44 × 10³pH 7.4, 30 °COpen
mitochondrial malate dehydrogenase (m-MDH)Talaromyces emersoniiNAD+native enzyme purified from T. emersonii mycelia2.77 × 10³0.122.3 × 10⁴pH 7.4, 30 °COpen
mitochondrial malate dehydrogenase (m-MDH)Talaromyces emersoniiNAD+expressed in E. coli BL21-AI; Ni-NTA purified3.1 × 10³0.132.74 × 10⁴pH 7.4, 30 °COpen
mitochondrial malate dehydrogenase (m-MDH)Talaromyces emersoniiNADHnative enzyme purified from T. emersonii mycelia1.1 × 10⁵0.0254.4 × 10⁶pH 7.4, 30 °COpen
mitochondrial malate dehydrogenase (m-MDH)Talaromyces emersoniiNADHexpressed in E. coli BL21-AI; Ni-NTA purified9 × 10⁴0.0294.8 × 10⁶pH 7.4, 30 °COpen
mitochondrial malate dehydrogenase (m-MDH)Talaromyces emersoniiOxaloacetatenative enzyme purified from T. emersonii mycelia9.1 × 10⁴0.024.5 × 10⁶pH 7.4, 30 °COpen
mitochondrial malate dehydrogenase (m-MDH)Talaromyces emersoniiOxaloacetateexpressed in E. coli BL21-AI; Ni-NTA purified8.1 × 10⁴0.0225.1 × 10⁶pH 7.4, 30 °COpen