CatLogenzyme kinetics catalog

Values CatLog read from this paper

Residues Lys-149 and Glu-153 switch the aminoacylation of tRNA(Trp) in Bacillus subtilis

PMID 15280378doi:10.1074/jbc.m401937200Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 40 records (120 values) from this paper: tryptophanyl-tRNA synthetase (TrpRS), tryptophanyl-tRNA synthetase (human TrpRS) from Bacillus subtilis, Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 6.1.1.2

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
tryptophanyl-tRNA synthetase (human TrpRS)Homo sapiensB. subtilis tRNA^Trp0.010.0011810pH 7.5, 22 °COpen
tryptophanyl-tRNA synthetase (human TrpRS)Homo sapienshuman tRNA^Trp1.330.001211103pH 7.5, 22 °COpen
tryptophanyl-tRNA synthetase (TrpRS)Bacillus subtilisATPATP-pyrophosphate exchange (Trp activation)220.065340pH 9, 25 °COpen
tryptophanyl-tRNA synthetase (TrpRS)Bacillus subtilisATPATP varied with L-Trp fixed at 2 mM as for Table I470.102460pH 9, 25 °COpen
tryptophanyl-tRNA synthetase (TrpRS)Bacillus subtilisB. subtilis tRNA^Trp0.716.1 × 10⁻⁴1.16 × 10³pH 7.5, 22 °COpen
tryptophanyl-tRNA synthetase (TrpRS)Bacillus subtilishuman tRNA^Trp0.01060.001218.8pH 7.5, 22 °COpen
tryptophanyl-tRNA synthetase (TrpRS)Bacillus subtilisL-tryptophanATP-pyrophosphate exchange (Trp activation)220.00464.79 × 10³pH 9, 25 °COpen
tryptophanyl-tRNA synthetase (TrpRS)Bacillus subtilisL-tryptophanL-Trp varied with ATP fixed at 4 mM as for Table I470.0067.78 × 10³pH 9, 25 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant E153DBacillus subtilisATP170.048370pH 9, 25 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant E153DBacillus subtilisB. subtilis tRNA^Trp0.788.8 × 10⁻⁴890pH 7.5, 22 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant E153DBacillus subtilishuman tRNA^Trp0.00630.001195.3pH 7.5, 22 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant E153DBacillus subtilisL-tryptophan180.00335.45 × 10³pH 9, 25 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant E153GBacillus subtilisATP420.091460pH 9, 25 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant E153GBacillus subtilisB. subtilis tRNA^Trp0.020.0012320pH 7.5, 22 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant E153GBacillus subtilishuman tRNA^Trp0.00130.001071.2pH 7.5, 22 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant E153GBacillus subtilisL-tryptophan420.0057.77 × 10³pH 9, 25 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant E153KBacillus subtilisATPATP-pyrophosphate exchange (Trp activation)4191.552270pH 9, 25 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant E153KBacillus subtilisATPATP varied with L-Trp fixed at 2 mM as for Table I1290.286450pH 9, 25 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant E153KBacillus subtilisB. subtilis tRNA^Trp0.030.0011330pH 7.5, 22 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant E153KBacillus subtilishuman tRNA^Trp0.01059.4 × 10⁻⁵112.1pH 7.5, 22 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant E153KBacillus subtilisL-tryptophanATP-pyrophosphate exchange (Trp activation)4190.1283.27 × 10³pH 9, 25 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant E153KBacillus subtilisL-tryptophanL-Trp varied with ATP fixed at 4 mM as for Table I1290.0167.78 × 10³pH 9, 25 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant K149D/E153R (WBHA)Bacillus subtilisATPATP-pyrophosphate exchange (Trp activation)270.278100pH 9, 25 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant K149D/E153R (WBHA)Bacillus subtilisATPATP varied with L-Trp fixed at 2 mM as for Table I630.136460pH 9, 25 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant K149D/E153R (WBHA)Bacillus subtilisB. subtilis tRNA^Trp0.0160.0012610pH 7.5, 22 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant K149D/E153R (WBHA)Bacillus subtilishuman tRNA^Trp0.01254.7 × 10⁻⁵267pH 7.5, 22 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant K149D/E153R (WBHA)Bacillus subtilisL-tryptophanATP-pyrophosphate exchange (Trp activation)280.06460pH 9, 25 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant K149D/E153R (WBHA)Bacillus subtilisL-tryptophanL-Trp varied with ATP fixed at 4 mM as for Table I630.00817.75 × 10³pH 9, 25 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant K149EBacillus subtilisATP160.046340pH 9, 25 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant K149EBacillus subtilisB. subtilis tRNA^Trp0.328.7 × 10⁻⁴370pH 7.5, 22 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant K149EBacillus subtilishuman tRNA^Trp0.076 × 10⁻⁴117.8pH 7.5, 22 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant K149EBacillus subtilisL-tryptophan160.00374.24 × 10³pH 9, 25 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant K149GBacillus subtilisATP150.041380pH 9, 25 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant K149GBacillus subtilisB. subtilis tRNA^Trp0.670.00121550pH 7.5, 22 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant K149GBacillus subtilishuman tRNA^Trp0.01180.001269.3pH 7.5, 22 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant K149GBacillus subtilisL-tryptophan150.00384.21 × 10³pH 9, 25 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant K149RBacillus subtilisATP460.121380pH 9, 25 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant K149RBacillus subtilisB. subtilis tRNA^Trp0.325 × 10⁻⁴640pH 7.5, 22 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant K149RBacillus subtilishuman tRNA^Trp0.00580.001194.9pH 7.5, 22 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant K149RBacillus subtilisL-tryptophan460.00994.67 × 10³pH 9, 25 °COpen