EC 6.1.1.2: tryptophan-tRNA ligase
LigasesOpen in Browse
CatLog read 135 records for EC 6.1.1.2 from 5 papers, for 4 organisms. Names in these papers: tryptophanyl-tRNA synthetase (TrpRS), tryptophanyl-tRNA synthetase (A. fulgidus TrpRS), tryptophanyl-tRNA synthetase (B. subtilis TrpRS).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 34 records for EC 6.1.1.2 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 6.1.1.2 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Escherichia coli tryptophanyl-tRNA synthetase mutants selected for tryptophan auxotrophy implicate the dimer interface in optimizing amino acid binding | Escherichia coli | 43 |
| Residues Lys-149 and Glu-153 switch the aminoacylation of tRNA(Trp) in Bacillus subtilis | Bacillus subtilis | 38 |
| Recognition by tryptophanyl-tRNA synthetases of discriminator base on tRNATrp from three biological domains | Archaeoglobus fulgidus, Bacillus subtilis | 24 |
| Molecular recognition of tryptophan tRNA by tryptophanyl-tRNA synthetase from Aeropyrum pernix K1 | Aeropyrum pernix | 23 |
| Two essential regions for tRNA recognition in Bacillus subtilis tryptophanyl-tRNA synthetase | Bacillus subtilis | 7 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| tryptophanyl-tRNA synthetase (A. fulgidus TrpRS) | Archaeoglobus fulgidus | A. fulgidus tRNATrp (A73, wild type) | 0.53 | 6 × 10⁻⁵ | 8.8 × 10³ calc. | pH 7, 65 °C | PMID 11834741 | Open record: tryptophanyl-tRNA synthetase (A. fulgidus TrpRS), A. fulgidus tRNATrp (A73, wild type) |
| tryptophanyl-tRNA synthetase (A. fulgidus TrpRS) | Archaeoglobus fulgidus | A. fulgidus tRNATrp A73C mutant | 0.11 | 5 × 10⁻⁵ | 2.2 × 10³ calc. | pH 7, 65 °C | PMID 11834741 | Open record: tryptophanyl-tRNA synthetase (A. fulgidus TrpRS), A. fulgidus tRNATrp A73C mutant |
| tryptophanyl-tRNA synthetase (A. fulgidus TrpRS) | Archaeoglobus fulgidus | A. fulgidus tRNATrp A73G mutant | 0.07 | 6 × 10⁻⁵ | 1.2 × 10³ calc. | pH 7, 65 °C | PMID 11834741 | Open record: tryptophanyl-tRNA synthetase (A. fulgidus TrpRS), A. fulgidus tRNATrp A73G mutant |
| tryptophanyl-tRNA synthetase (A. fulgidus TrpRS) | Archaeoglobus fulgidus | A. fulgidus tRNATrp A73U mutant | 0.09 | 5 × 10⁻⁵ | 1.8 × 10³ calc. | pH 7, 65 °C | PMID 11834741 | Open record: tryptophanyl-tRNA synthetase (A. fulgidus TrpRS), A. fulgidus tRNATrp A73U mutant |
| tryptophanyl-tRNA synthetase (A. fulgidus TrpRS) | Archaeoglobus fulgidus | B. subtilis tRNATrp (G73, wild type) | 0.04 | 1 × 10⁻⁴ | 400 calc. | pH 7, 65 °C | PMID 11834741 | Open record: tryptophanyl-tRNA synthetase (A. fulgidus TrpRS), B. subtilis tRNATrp (G73, wild type) |
| tryptophanyl-tRNA synthetase (A. fulgidus TrpRS) | Archaeoglobus fulgidus | B. subtilis tRNATrp G73A mutant | 0.14 | 9 × 10⁻⁵ | 1.6 × 10³ calc. | pH 7, 65 °C | PMID 11834741 | Open record: tryptophanyl-tRNA synthetase (A. fulgidus TrpRS), B. subtilis tRNATrp G73A mutant |
| tryptophanyl-tRNA synthetase (A. fulgidus TrpRS) | Archaeoglobus fulgidus | B. subtilis tRNATrp G73C mutant | 0.06 | 1.2 × 10⁻⁴ | 500 calc. | pH 7, 65 °C | PMID 11834741 | Open record: tryptophanyl-tRNA synthetase (A. fulgidus TrpRS), B. subtilis tRNATrp G73C mutant |
| tryptophanyl-tRNA synthetase (A. fulgidus TrpRS) | Archaeoglobus fulgidus | B. subtilis tRNATrp G73U mutant | 0.05 | 1.2 × 10⁻⁴ | 420 calc. | pH 7, 65 °C | PMID 11834741 | Open record: tryptophanyl-tRNA synthetase (A. fulgidus TrpRS), B. subtilis tRNATrp G73U mutant |
| tryptophanyl-tRNA synthetase (A. fulgidus TrpRS) | Archaeoglobus fulgidus | bovine tRNATrp (A73, wild type) | 0.08 | 2.1 × 10⁻⁴ | 380 calc. | pH 7, 65 °C | PMID 11834741 | Open record: tryptophanyl-tRNA synthetase (A. fulgidus TrpRS), bovine tRNATrp (A73, wild type) |
| tryptophanyl-tRNA synthetase (A. fulgidus TrpRS) | Archaeoglobus fulgidus | bovine tRNATrp A73C mutant | 0.06 | 2.2 × 10⁻⁴ | 270 calc. | pH 7, 65 °C | PMID 11834741 | Open record: tryptophanyl-tRNA synthetase (A. fulgidus TrpRS), bovine tRNATrp A73C mutant |
| tryptophanyl-tRNA synthetase (A. fulgidus TrpRS) | Archaeoglobus fulgidus | bovine tRNATrp A73G mutant | 0.03 | 1.8 × 10⁻⁴ | 170 calc. | pH 7, 65 °C | PMID 11834741 | Open record: tryptophanyl-tRNA synthetase (A. fulgidus TrpRS), bovine tRNATrp A73G mutant |
| tryptophanyl-tRNA synthetase (A. fulgidus TrpRS) | Archaeoglobus fulgidus | bovine tRNATrp A73U mutant | 0.04 | 1.7 × 10⁻⁴ | 240 calc. | pH 7, 65 °C | PMID 11834741 | Open record: tryptophanyl-tRNA synthetase (A. fulgidus TrpRS), bovine tRNATrp A73U mutant |
| tryptophanyl-tRNA synthetase (B. subtilis TrpRS) | Bacillus subtilis | A. fulgidus tRNATrp (A73, wild type) | 0.32 | 1.2 × 10⁻⁴ | 2.7 × 10³ calc. | 22 °C | PMID 11834741 | Open record: tryptophanyl-tRNA synthetase (B. subtilis TrpRS), A. fulgidus tRNATrp (A73, wild type) |
| tryptophanyl-tRNA synthetase (B. subtilis TrpRS) | Bacillus subtilis | A. fulgidus tRNATrp A73C mutant | 0.21 | 1.1 × 10⁻⁴ | 1.9 × 10³ calc. | 22 °C | PMID 11834741 | Open record: tryptophanyl-tRNA synthetase (B. subtilis TrpRS), A. fulgidus tRNATrp A73C mutant |
| tryptophanyl-tRNA synthetase (B. subtilis TrpRS) | Bacillus subtilis | A. fulgidus tRNATrp A73G mutant | 0.57 | 1 × 10⁻⁴ | 5.7 × 10³ calc. | 22 °C | PMID 11834741 | Open record: tryptophanyl-tRNA synthetase (B. subtilis TrpRS), A. fulgidus tRNATrp A73G mutant |
| tryptophanyl-tRNA synthetase (B. subtilis TrpRS) | Bacillus subtilis | A. fulgidus tRNATrp A73U mutant | 0.26 | 1.3 × 10⁻⁴ | 2.0 × 10³ calc. | 22 °C | PMID 11834741 | Open record: tryptophanyl-tRNA synthetase (B. subtilis TrpRS), A. fulgidus tRNATrp A73U mutant |
| tryptophanyl-tRNA synthetase (B. subtilis TrpRS) | Bacillus subtilis | B. subtilis tRNATrp (G73, wild type) | 5.09 | 2 × 10⁻⁵ | 2.5 × 10⁵ calc. | 22 °C | PMID 11834741 | Open record: tryptophanyl-tRNA synthetase (B. subtilis TrpRS), B. subtilis tRNATrp (G73, wild type) |
| tryptophanyl-tRNA synthetase (B. subtilis TrpRS) | Bacillus subtilis | B. subtilis tRNATrp G73A mutant | 0.81 | 3 × 10⁻⁵ | 2.7 × 10⁴ calc. | 22 °C | PMID 11834741 | Open record: tryptophanyl-tRNA synthetase (B. subtilis TrpRS), B. subtilis tRNATrp G73A mutant |
| tryptophanyl-tRNA synthetase (B. subtilis TrpRS) | Bacillus subtilis | B. subtilis tRNATrp G73C mutant | 0.27 | 2 × 10⁻⁵ | 1.4 × 10⁴ calc. | 22 °C | PMID 11834741 | Open record: tryptophanyl-tRNA synthetase (B. subtilis TrpRS), B. subtilis tRNATrp G73C mutant |
| tryptophanyl-tRNA synthetase (B. subtilis TrpRS) | Bacillus subtilis | B. subtilis tRNATrp G73U mutant | 0.46 | 3 × 10⁻⁵ | 1.5 × 10⁴ calc. | 22 °C | PMID 11834741 | Open record: tryptophanyl-tRNA synthetase (B. subtilis TrpRS), B. subtilis tRNATrp G73U mutant |
| tryptophanyl-tRNA synthetase (B. subtilis TrpRS) | Bacillus subtilis | bovine tRNATrp (A73, wild type) | 0.21 | 1.6 × 10⁻⁴ | 1.3 × 10³ calc. | 22 °C | PMID 11834741 | Open record: tryptophanyl-tRNA synthetase (B. subtilis TrpRS), bovine tRNATrp (A73, wild type) |
| tryptophanyl-tRNA synthetase (B. subtilis TrpRS) | Bacillus subtilis | bovine tRNATrp A73C mutant | 0.12 | 1.7 × 10⁻⁴ | 710 calc. | 22 °C | PMID 11834741 | Open record: tryptophanyl-tRNA synthetase (B. subtilis TrpRS), bovine tRNATrp A73C mutant |
| tryptophanyl-tRNA synthetase (B. subtilis TrpRS) | Bacillus subtilis | bovine tRNATrp A73G mutant | 0.48 | 1.5 × 10⁻⁴ | 3.2 × 10³ calc. | 22 °C | PMID 11834741 | Open record: tryptophanyl-tRNA synthetase (B. subtilis TrpRS), bovine tRNATrp A73G mutant |
| tryptophanyl-tRNA synthetase (B. subtilis TrpRS) | Bacillus subtilis | bovine tRNATrp A73U mutant | 0.18 | 1.6 × 10⁻⁴ | 1.1 × 10³ calc. | 22 °C | PMID 11834741 | Open record: tryptophanyl-tRNA synthetase (B. subtilis TrpRS), bovine tRNATrp A73U mutant |
| tryptophanyl-tRNA synthetase (TrpRS) | Aeropyrum pernix | Aeropyrum pernix arginine tRNA (tRNAArg, anticodon CCU), wild-type in vitro transcript | 0.16 | 0.0021 | 0.78 | pH 7.5, 60 °C | PMID 19179361 | Open record: tryptophanyl-tRNA synthetase (TrpRS), Aeropyrum pernix arginine tRNA (tRNAArg, anticodon CCU), wild-type in vitro transcript |
| tryptophanyl-tRNA synthetase (TrpRS) | Aeropyrum pernix | Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, A36C mutant | 11 | 0.0059 | 18 | pH 7.5, 60 °C | PMID 19179361 | Open record: tryptophanyl-tRNA synthetase (TrpRS), Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, A36C mutant |
| tryptophanyl-tRNA synthetase (TrpRS) | Aeropyrum pernix | Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, A36G mutant | 4.4 | 0.0053 | 8.4 | pH 7.5, 60 °C | PMID 19179361 | Open record: tryptophanyl-tRNA synthetase (TrpRS), Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, A36G mutant |
| tryptophanyl-tRNA synthetase (TrpRS) | Aeropyrum pernix | Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, A36U mutant | 21 | 0.0058 | 36 | pH 7.5, 60 °C | PMID 19179361 | Open record: tryptophanyl-tRNA synthetase (TrpRS), Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, A36U mutant |
| tryptophanyl-tRNA synthetase (TrpRS) | Aeropyrum pernix | Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, A36U, A73G mutant | 0.27 | 0.0098 | 0.28 | pH 7.5, 60 °C | PMID 19179361 | Open record: tryptophanyl-tRNA synthetase (TrpRS), Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, A36U, A73G mutant |
| tryptophanyl-tRNA synthetase (TrpRS) | Aeropyrum pernix | Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, A73C mutant | 5.5 | 0.0017 | 34 | pH 7.5, 60 °C | PMID 19179361 | Open record: tryptophanyl-tRNA synthetase (TrpRS), Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, A73C mutant |
| tryptophanyl-tRNA synthetase (TrpRS) | Aeropyrum pernix | Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, A73G mutant | 0.62 | 0.0058 | 1.1 | pH 7.5, 60 °C | PMID 19179361 | Open record: tryptophanyl-tRNA synthetase (TrpRS), Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, A73G mutant |
| tryptophanyl-tRNA synthetase (TrpRS) | Aeropyrum pernix | Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, A73U mutant | 6.1 | 0.0051 | 12 | pH 7.5, 60 °C | PMID 19179361 | Open record: tryptophanyl-tRNA synthetase (TrpRS), Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, A73U mutant |
| tryptophanyl-tRNA synthetase (TrpRS) | Aeropyrum pernix | Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, C34A mutant | 0.17 | 0.06 | 0.028 | pH 7.5, 60 °C | PMID 19179361 | Open record: tryptophanyl-tRNA synthetase (TrpRS), Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, C34A mutant |
| tryptophanyl-tRNA synthetase (TrpRS) | Aeropyrum pernix | Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, C34G mutant | 0.13 | 0.038 | 0.033 | pH 7.5, 60 °C | PMID 19179361 | Open record: tryptophanyl-tRNA synthetase (TrpRS), Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, C34G mutant |
| tryptophanyl-tRNA synthetase (TrpRS) | Aeropyrum pernix | Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, C34U mutant | 0.11 | 0.048 | 0.024 | pH 7.5, 60 °C | PMID 19179361 | Open record: tryptophanyl-tRNA synthetase (TrpRS), Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, C34U mutant |
| tryptophanyl-tRNA synthetase (TrpRS) | Aeropyrum pernix | Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, C35A mutant | 0.15 | 0.014 | 0.11 | pH 7.5, 60 °C | PMID 19179361 | Open record: tryptophanyl-tRNA synthetase (TrpRS), Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, C35A mutant |
| tryptophanyl-tRNA synthetase (TrpRS) | Aeropyrum pernix | Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, C35G mutant | 0.079 | 0.038 | 0.021 | pH 7.5, 60 °C | PMID 19179361 | Open record: tryptophanyl-tRNA synthetase (TrpRS), Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, C35G mutant |
| tryptophanyl-tRNA synthetase (TrpRS) | Aeropyrum pernix | Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, C35U mutant | 0.47 | 0.021 | 0.22 | pH 7.5, 60 °C | PMID 19179361 | Open record: tryptophanyl-tRNA synthetase (TrpRS), Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, C35U mutant |
| tryptophanyl-tRNA synthetase (TrpRS) | Aeropyrum pernix | Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, G1A–C72U mutant | 2.3 | 0.025 | 0.93 | pH 7.5, 60 °C | PMID 19179361 | Open record: tryptophanyl-tRNA synthetase (TrpRS), Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, G1A–C72U mutant |
| tryptophanyl-tRNA synthetase (TrpRS) | Aeropyrum pernix | Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, G1C–G72G mutant | 0.34 | 0.0055 | 0.61 | pH 7.5, 60 °C | PMID 19179361 | Open record: tryptophanyl-tRNA synthetase (TrpRS), Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, G1C–G72G mutant |
| tryptophanyl-tRNA synthetase (TrpRS) | Aeropyrum pernix | Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, G1U–C72A mutant | 0.46 | 0.021 | 0.22 | pH 7.5, 60 °C | PMID 19179361 | Open record: tryptophanyl-tRNA synthetase (TrpRS), Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, G1U–C72A mutant |
| tryptophanyl-tRNA synthetase (TrpRS) | Aeropyrum pernix | Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, G2A–C71U mutant | 19 | 0.015 | 13 | pH 7.5, 60 °C | PMID 19179361 | Open record: tryptophanyl-tRNA synthetase (TrpRS), Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, G2A–C71U mutant |
| tryptophanyl-tRNA synthetase (TrpRS) | Aeropyrum pernix | Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, G2C–G71G mutant | 1.7 | 0.0019 | 9.4 | pH 7.5, 60 °C | PMID 19179361 | Open record: tryptophanyl-tRNA synthetase (TrpRS), Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, G2C–G71G mutant |
| tryptophanyl-tRNA synthetase (TrpRS) | Aeropyrum pernix | Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, G2U–C71A mutant | 7.2 | 0.0058 | 13 | pH 7.5, 60 °C | PMID 19179361 | Open record: tryptophanyl-tRNA synthetase (TrpRS), Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, G2U–C71A mutant |
| tryptophanyl-tRNA synthetase (TrpRS) | Aeropyrum pernix | Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, HOG1 mutant | 3.2 | 0.006 | 5.4 | pH 7.5, 60 °C | PMID 19179361 | Open record: tryptophanyl-tRNA synthetase (TrpRS), Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, HOG1 mutant |
| tryptophanyl-tRNA synthetase (TrpRS) | Aeropyrum pernix | Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, pppG1 mutant | 4.5 | 0.012 | 4 | pH 7.5, 60 °C | PMID 19179361 | Open record: tryptophanyl-tRNA synthetase (TrpRS), Aeropyrum pernix tryptophan tRNA (tRNATrp) in vitro transcript, pppG1 mutant |
| tryptophanyl-tRNA synthetase (TrpRS) | Aeropyrum pernix | Aeropyrum pernix tryptophan tRNA (tRNATrp), wild-type in vitro transcript | 16 | 0.0012 | 140 | pH 7.5, 60 °C | PMID 19179361 | Open record: tryptophanyl-tRNA synthetase (TrpRS), Aeropyrum pernix tryptophan tRNA (tRNATrp), wild-type in vitro transcript |
| tryptophanyl-tRNA synthetase (TrpRS) | Bacillus subtilis | ATPATP-pyrophosphate exchange (Trp activation) | 22 | 0.065 | 340 | pH 9, 25 °C | PMID 15280378 | Open record: tryptophanyl-tRNA synthetase (TrpRS), ATP |
| tryptophanyl-tRNA synthetase (TrpRS) | Bacillus subtilis | ATPATP varied with L-Trp fixed at 2 mM as for Table I | 47 | 0.102 | 460 | pH 9, 25 °C | PMID 15280378 | Open record: tryptophanyl-tRNA synthetase (TrpRS), ATP |
| tryptophanyl-tRNA synthetase (TrpRS) | Bacillus subtilis | B. subtilis tRNA^Trp | 0.71 | 6.1 × 10⁻⁴ | 1.16 × 10³ | pH 7.5, 22 °C | PMID 15280378 | Open record: tryptophanyl-tRNA synthetase (TrpRS), B. subtilis tRNA^Trp |
| tryptophanyl-tRNA synthetase (TrpRS) | Bacillus subtilis | human tRNA^Trp | 0.0106 | 0.00121 | 8.8 | pH 7.5, 22 °C | PMID 15280378 | Open record: tryptophanyl-tRNA synthetase (TrpRS), human tRNA^Trp |
| tryptophanyl-tRNA synthetase (TrpRS) | Bacillus subtilis | L-tryptophan | 0.9 | 7.3 × 10⁻⁴ | 1.24 × 10³ | pH 7.5, 22 °C | PMID 11966471 | Open record: tryptophanyl-tRNA synthetase (TrpRS), L-tryptophan |
| tryptophanyl-tRNA synthetase (TrpRS) | Bacillus subtilis | L-tryptophanATP-pyrophosphate exchange (Trp activation) | 22 | 0.0046 | 4.79 × 10³ | pH 9, 25 °C | PMID 15280378 | Open record: tryptophanyl-tRNA synthetase (TrpRS), L-tryptophan |
| tryptophanyl-tRNA synthetase (TrpRS) | Bacillus subtilis | L-tryptophanL-Trp varied with ATP fixed at 4 mM as for Table I | 47 | 0.006 | 7.78 × 10³ | pH 9, 25 °C | PMID 15280378 | Open record: tryptophanyl-tRNA synthetase (TrpRS), L-tryptophan |
| tryptophanyl-tRNA synthetase (TrpRS) | Bacillus subtilis | tRNATrp | 0.9 | 1.8 × 10⁻⁴ | 5.02 × 10³ | pH 7.5, 22 °C | PMID 11966471 | Open record: tryptophanyl-tRNA synthetase (TrpRS), tRNATrp |
| tryptophanyl-tRNA synthetase (TrpRS)variant E153D | Bacillus subtilis | ATP | 17 | 0.048 | 370 | pH 9, 25 °C | PMID 15280378 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant E153D, ATP |
| tryptophanyl-tRNA synthetase (TrpRS)variant E153D | Bacillus subtilis | B. subtilis tRNA^Trp | 0.78 | 8.8 × 10⁻⁴ | 890 | pH 7.5, 22 °C | PMID 15280378 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant E153D, B. subtilis tRNA^Trp |
| tryptophanyl-tRNA synthetase (TrpRS)variant E153D | Bacillus subtilis | human tRNA^Trp | 0.0063 | 0.00119 | 5.3 | pH 7.5, 22 °C | PMID 15280378 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant E153D, human tRNA^Trp |
| tryptophanyl-tRNA synthetase (TrpRS)variant E153D | Bacillus subtilis | L-tryptophan | 18 | 0.0033 | 5.45 × 10³ | pH 9, 25 °C | PMID 15280378 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant E153D, L-tryptophan |
| tryptophanyl-tRNA synthetase (TrpRS)variant E153G | Bacillus subtilis | ATP | 42 | 0.091 | 460 | pH 9, 25 °C | PMID 15280378 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant E153G, ATP |
| tryptophanyl-tRNA synthetase (TrpRS)variant E153G | Bacillus subtilis | B. subtilis tRNA^Trp | 0.02 | 0.00123 | 20 | pH 7.5, 22 °C | PMID 15280378 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant E153G, B. subtilis tRNA^Trp |
| tryptophanyl-tRNA synthetase (TrpRS)variant E153G | Bacillus subtilis | human tRNA^Trp | 0.0013 | 0.00107 | 1.2 | pH 7.5, 22 °C | PMID 15280378 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant E153G, human tRNA^Trp |
| tryptophanyl-tRNA synthetase (TrpRS)variant E153G | Bacillus subtilis | L-tryptophan | 42 | 0.005 | 7.77 × 10³ | pH 9, 25 °C | PMID 15280378 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant E153G, L-tryptophan |
| tryptophanyl-tRNA synthetase (TrpRS)variant E153K | Bacillus subtilis | ATPATP-pyrophosphate exchange (Trp activation) | 419 | 1.552 | 270 | pH 9, 25 °C | PMID 15280378 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant E153K, ATP |
| tryptophanyl-tRNA synthetase (TrpRS)variant E153K | Bacillus subtilis | ATPATP varied with L-Trp fixed at 2 mM as for Table I | 129 | 0.286 | 450 | pH 9, 25 °C | PMID 15280378 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant E153K, ATP |
| tryptophanyl-tRNA synthetase (TrpRS)variant E153K | Bacillus subtilis | B. subtilis tRNA^Trp | 0.03 | 0.00113 | 30 | pH 7.5, 22 °C | PMID 15280378 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant E153K, B. subtilis tRNA^Trp |
| tryptophanyl-tRNA synthetase (TrpRS)variant E153K | Bacillus subtilis | human tRNA^Trp | 0.0105 | 9.4 × 10⁻⁵ | 112.1 | pH 7.5, 22 °C | PMID 15280378 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant E153K, human tRNA^Trp |
| tryptophanyl-tRNA synthetase (TrpRS)variant E153K | Bacillus subtilis | L-tryptophanATP-pyrophosphate exchange (Trp activation) | 419 | 0.128 | 3.27 × 10³ | pH 9, 25 °C | PMID 15280378 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant E153K, L-tryptophan |
| tryptophanyl-tRNA synthetase (TrpRS)variant E153K | Bacillus subtilis | L-tryptophanL-Trp varied with ATP fixed at 4 mM as for Table I | 129 | 0.016 | 7.78 × 10³ | pH 9, 25 °C | PMID 15280378 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant E153K, L-tryptophan |
| tryptophanyl-tRNA synthetase (TrpRS)variant K149D/E153R (WBHA) | Bacillus subtilis | ATPATP-pyrophosphate exchange (Trp activation) | 27 | 0.278 | 100 | pH 9, 25 °C | PMID 15280378 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant K149D/E153R (WBHA), ATP |
| tryptophanyl-tRNA synthetase (TrpRS)variant K149D/E153R (WBHA) | Bacillus subtilis | ATPATP varied with L-Trp fixed at 2 mM as for Table I | 63 | 0.136 | 460 | pH 9, 25 °C | PMID 15280378 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant K149D/E153R (WBHA), ATP |
| tryptophanyl-tRNA synthetase (TrpRS)variant K149D/E153R (WBHA) | Bacillus subtilis | B. subtilis tRNA^Trp | 0.016 | 0.00126 | 10 | pH 7.5, 22 °C | PMID 15280378 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant K149D/E153R (WBHA), B. subtilis tRNA^Trp |
| tryptophanyl-tRNA synthetase (TrpRS)variant K149D/E153R (WBHA) | Bacillus subtilis | human tRNA^Trp | 0.0125 | 4.7 × 10⁻⁵ | 267 | pH 7.5, 22 °C | PMID 15280378 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant K149D/E153R (WBHA), human tRNA^Trp |
| tryptophanyl-tRNA synthetase (TrpRS)variant K149D/E153R (WBHA) | Bacillus subtilis | L-tryptophanATP-pyrophosphate exchange (Trp activation) | 28 | 0.06 | 460 | pH 9, 25 °C | PMID 15280378 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant K149D/E153R (WBHA), L-tryptophan |
| tryptophanyl-tRNA synthetase (TrpRS)variant K149D/E153R (WBHA) | Bacillus subtilis | L-tryptophanL-Trp varied with ATP fixed at 4 mM as for Table I | 63 | 0.0081 | 7.75 × 10³ | pH 9, 25 °C | PMID 15280378 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant K149D/E153R (WBHA), L-tryptophan |
| tryptophanyl-tRNA synthetase (TrpRS)variant K149E | Bacillus subtilis | ATP | 16 | 0.046 | 340 | pH 9, 25 °C | PMID 15280378 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant K149E, ATP |
| tryptophanyl-tRNA synthetase (TrpRS)variant K149E | Bacillus subtilis | B. subtilis tRNA^Trp | 0.32 | 8.7 × 10⁻⁴ | 370 | pH 7.5, 22 °C | PMID 15280378 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant K149E, B. subtilis tRNA^Trp |
| tryptophanyl-tRNA synthetase (TrpRS)variant K149E | Bacillus subtilis | human tRNA^Trp | 0.07 | 6 × 10⁻⁴ | 117.8 | pH 7.5, 22 °C | PMID 15280378 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant K149E, human tRNA^Trp |
| tryptophanyl-tRNA synthetase (TrpRS)variant K149E | Bacillus subtilis | L-tryptophan | 16 | 0.0037 | 4.24 × 10³ | pH 9, 25 °C | PMID 15280378 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant K149E, L-tryptophan |
| tryptophanyl-tRNA synthetase (TrpRS)variant K149G | Bacillus subtilis | ATP | 15 | 0.041 | 380 | pH 9, 25 °C | PMID 15280378 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant K149G, ATP |
| tryptophanyl-tRNA synthetase (TrpRS)variant K149G | Bacillus subtilis | B. subtilis tRNA^Trp | 0.67 | 0.00121 | 550 | pH 7.5, 22 °C | PMID 15280378 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant K149G, B. subtilis tRNA^Trp |
| tryptophanyl-tRNA synthetase (TrpRS)variant K149G | Bacillus subtilis | human tRNA^Trp | 0.0118 | 0.00126 | 9.3 | pH 7.5, 22 °C | PMID 15280378 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant K149G, human tRNA^Trp |
| tryptophanyl-tRNA synthetase (TrpRS)variant K149G | Bacillus subtilis | L-tryptophan | 15 | 0.0038 | 4.21 × 10³ | pH 9, 25 °C | PMID 15280378 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant K149G, L-tryptophan |
| tryptophanyl-tRNA synthetase (TrpRS)variant K149R | Bacillus subtilis | ATP | 46 | 0.121 | 380 | pH 9, 25 °C | PMID 15280378 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant K149R, ATP |
| tryptophanyl-tRNA synthetase (TrpRS)variant K149R | Bacillus subtilis | B. subtilis tRNA^Trp | 0.32 | 5 × 10⁻⁴ | 640 | pH 7.5, 22 °C | PMID 15280378 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant K149R, B. subtilis tRNA^Trp |
| tryptophanyl-tRNA synthetase (TrpRS)variant K149R | Bacillus subtilis | human tRNA^Trp | 0.0058 | 0.00119 | 4.9 | pH 7.5, 22 °C | PMID 15280378 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant K149R, human tRNA^Trp |
| tryptophanyl-tRNA synthetase (TrpRS)variant K149R | Bacillus subtilis | L-tryptophan | 46 | 0.0099 | 4.67 × 10³ | pH 9, 25 °C | PMID 15280378 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant K149R, L-tryptophan |
| tryptophanyl-tRNA synthetase (TrpRS)variant Δ108-122 | Bacillus subtilis | L-tryptophan | 0.61 | 0.00212 | 290 | pH 7.5, 22 °C | PMID 11966471 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant Δ108-122, L-tryptophan |
| tryptophanyl-tRNA synthetase (TrpRS)variant Δ108-122 | Bacillus subtilis | tRNATrp | 0.61 | 2.2 × 10⁻⁴ | 2.78 × 10³ | pH 7.5, 22 °C | PMID 11966471 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant Δ108-122, tRNATrp |
| tryptophanyl-tRNA synthetase (TrpRS)variant Δ108-122/Δ234-238 | Bacillus subtilis | L-tryptophan | 0.4 | 0.00238 | 170 | pH 7.5, 22 °C | PMID 11966471 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant Δ108-122/Δ234-238, L-tryptophan |
| tryptophanyl-tRNA synthetase (TrpRS)variant Δ234-238 | Bacillus subtilis | L-tryptophan | 0.59 | 0.00234 | 250 | pH 7.5, 22 °C | PMID 11966471 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant Δ234-238, L-tryptophan |
| tryptophanyl-tRNA synthetase (TrpRS)variant Δ234-238 | Bacillus subtilis | tRNATrp | 0.59 | 5.2 × 10⁻⁴ | 1.13 × 10³ | pH 7.5, 22 °C | PMID 11966471 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant Δ234-238, tRNATrp |
| tryptophanyl-tRNA synthetase (TrpRS) | Escherichia coli | ATPaminoacylation assay with [3H]Trp (filter binding); 4 mM DTT | 1.5 | 0.19 | 7.9 calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS), ATP |
| tryptophanyl-tRNA synthetase (TrpRS) | Escherichia coli | ATP20-200 nM TrpRS; tetrasodium pyrophosphate | 111 | 0.132 | 841 calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS), ATP |
| tryptophanyl-tRNA synthetase (TrpRS) | Escherichia coli | L-tryptophanaminoacylation assay with [3H]Trp (filter binding); 4 mM DTT | 2 | 0.0124 | 160 calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS), L-tryptophan |
| tryptophanyl-tRNA synthetase (TrpRS) | Escherichia coli | L-tryptophan20-200 nM TrpRS; tetrasodium pyrophosphate | 111 | 0.0182 | 6.10 × 10³ calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS), L-tryptophan |
| tryptophanyl-tRNA synthetase (TrpRS) | Escherichia coli | tRNATrp | 2 | 3.4 × 10⁻⁴ | 5.9 × 10³ calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS), tRNATrp |
| tryptophanyl-tRNA synthetase (TrpRS)variant A133E | Escherichia coli | ATPaminoacylation assay with [3H]Trp (filter binding); 4 mM DTT | 0.1 | 0.177 | 0.56 calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant A133E, ATP |
| tryptophanyl-tRNA synthetase (TrpRS)variant A133E | Escherichia coli | ATP20-200 nM TrpRS; tetrasodium pyrophosphate | 40 | 0.333 | 120 calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant A133E, ATP |
| tryptophanyl-tRNA synthetase (TrpRS)variant A133E | Escherichia coli | L-tryptophanaminoacylation assay with [3H]Trp (filter binding); 4 mM DTT | 0.6 | 0.048 | 12 calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant A133E, L-tryptophan |
| tryptophanyl-tRNA synthetase (TrpRS)variant A133E | Escherichia coli | L-tryptophan20-200 nM TrpRS; tetrasodium pyrophosphate | 40 | 0.043 | 930 calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant A133E, L-tryptophan |
| tryptophanyl-tRNA synthetase (TrpRS)variant A133E | Escherichia coli | tRNATrp | 0.26 | 5.6 × 10⁻⁴ | 460 calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant A133E, tRNATrp |
| tryptophanyl-tRNA synthetase (TrpRS)variant D112E | Escherichia coli | ATP | 2.2 | 0.216 | 10 calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant D112E, ATP |
| tryptophanyl-tRNA synthetase (TrpRS)variant D112E | Escherichia coli | L-tryptophan | 2.2 | 0.0058 | 380 calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant D112E, L-tryptophan |
| tryptophanyl-tRNA synthetase (TrpRS)variant D112E | Escherichia coli | tRNATrp | 2.5 | 2.8 × 10⁻⁴ | 8.9 × 10³ calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant D112E, tRNATrp |
| tryptophanyl-tRNA synthetase (TrpRS)variant D112E/P129S | Escherichia coli | ATPaminoacylation assay with [3H]Trp (filter binding); 4 mM DTT | 0.1 | 0.225 | 0.44 calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant D112E/P129S, ATP |
| tryptophanyl-tRNA synthetase (TrpRS)variant D112E/P129S | Escherichia coli | ATP20-200 nM TrpRS; tetrasodium pyrophosphate | 12 | 0.246 | 49 calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant D112E/P129S, ATP |
| tryptophanyl-tRNA synthetase (TrpRS)variant D112E/P129S | Escherichia coli | L-tryptophanaminoacylation assay with [3H]Trp (filter binding); 4 mM DTT | 0.12 | 0.114 | 1.1 calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant D112E/P129S, L-tryptophan |
| tryptophanyl-tRNA synthetase (TrpRS)variant D112E/P129S | Escherichia coli | L-tryptophan20-200 nM TrpRS; tetrasodium pyrophosphate | 11.8 | 0.183 | 64.5 calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant D112E/P129S, L-tryptophan |
| tryptophanyl-tRNA synthetase (TrpRS)variant D112E/P129S | Escherichia coli | tRNATrp | 0.11 | 5.6 × 10⁻⁴ | 200 calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant D112E/P129S, tRNATrp |
| tryptophanyl-tRNA synthetase (TrpRS)variant G329S | Escherichia coli | ATPaminoacylation assay with [3H]Trp (filter binding); 4 mM DTT | 1.9 | 0.178 | 11 calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant G329S, ATP |
| tryptophanyl-tRNA synthetase (TrpRS)variant G329S | Escherichia coli | ATP20-200 nM TrpRS; tetrasodium pyrophosphate | 37 | 0.083 | 450 calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant G329S, ATP |
| tryptophanyl-tRNA synthetase (TrpRS)variant G329S | Escherichia coli | L-tryptophanaminoacylation assay with [3H]Trp (filter binding); 4 mM DTT | 2.2 | 0.215 | 10 calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant G329S, L-tryptophan |
| tryptophanyl-tRNA synthetase (TrpRS)variant G329S | Escherichia coli | L-tryptophan20-200 nM TrpRS; tetrasodium pyrophosphate | 60 | 0.071 | 850 calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant G329S, L-tryptophan |
| tryptophanyl-tRNA synthetase (TrpRS)variant G329S | Escherichia coli | tRNATrp | 1.05 | 6.5 × 10⁻⁴ | 1.6 × 10³ calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant G329S, tRNATrp |
| tryptophanyl-tRNA synthetase (TrpRS)variant L91F | Escherichia coli | ATPaminoacylation assay with [3H]Trp (filter binding); 4 mM DTT | 2.1 | 0.201 | 10 calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant L91F, ATP |
| tryptophanyl-tRNA synthetase (TrpRS)variant L91F | Escherichia coli | ATP20-200 nM TrpRS; tetrasodium pyrophosphate | 78 | 0.194 | 400 calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant L91F, ATP |
| tryptophanyl-tRNA synthetase (TrpRS)variant L91F | Escherichia coli | L-tryptophanaminoacylation assay with [3H]Trp (filter binding); 4 mM DTT | 2.4 | 0.074 | 32 calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant L91F, L-tryptophan |
| tryptophanyl-tRNA synthetase (TrpRS)variant L91F | Escherichia coli | L-tryptophan20-200 nM TrpRS; tetrasodium pyrophosphate | 75 | 0.046 | 1.6 × 10³ calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant L91F, L-tryptophan |
| tryptophanyl-tRNA synthetase (TrpRS)variant L91F | Escherichia coli | tRNATrp | 2.4 | 8.9 × 10⁻⁴ | 2.7 × 10³ calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant L91F, tRNATrp |
| tryptophanyl-tRNA synthetase (TrpRS)variant M196I | Escherichia coli | ATPaminoacylation assay with [3H]Trp (filter binding); 4 mM DTT | 0.02 | 0.183 | 0.11 calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant M196I, ATP |
| tryptophanyl-tRNA synthetase (TrpRS)variant M196I | Escherichia coli | ATP20-200 nM TrpRS; tetrasodium pyrophosphate | 1.8 | 0.298 | 6.0 calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant M196I, ATP |
| tryptophanyl-tRNA synthetase (TrpRS)variant M196I | Escherichia coli | L-tryptophanaminoacylation assay with [3H]Trp (filter binding); 4 mM DTT | 0.01 | 0.0164 | 0.61 calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant M196I, L-tryptophan |
| tryptophanyl-tRNA synthetase (TrpRS)variant M196I | Escherichia coli | L-tryptophan20-200 nM TrpRS; tetrasodium pyrophosphate | 1.7 | 0.023 | 74 calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant M196I, L-tryptophan |
| tryptophanyl-tRNA synthetase (TrpRS)variant M196I | Escherichia coli | tRNATrp | 0.02 | 2.7 × 10⁻⁴ | 74 calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant M196I, tRNATrp |
| tryptophanyl-tRNA synthetase (TrpRS)variant P129S | Escherichia coli | ATPaminoacylation assay with [3H]Trp (filter binding); 4 mM DTT | 0.2 | 0.285 | 0.70 calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant P129S, ATP |
| tryptophanyl-tRNA synthetase (TrpRS)variant P129S | Escherichia coli | ATP20-200 nM TrpRS; tetrasodium pyrophosphate | 23 | 0.281 | 82 calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant P129S, ATP |
| tryptophanyl-tRNA synthetase (TrpRS)variant P129S | Escherichia coli | L-tryptophanaminoacylation assay with [3H]Trp (filter binding); 4 mM DTT | 0.1 | 0.096 | 1.0 calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant P129S, L-tryptophan |
| tryptophanyl-tRNA synthetase (TrpRS)variant P129S | Escherichia coli | L-tryptophan20-200 nM TrpRS; tetrasodium pyrophosphate | 13 | 0.2 | 65 calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant P129S, L-tryptophan |
| tryptophanyl-tRNA synthetase (TrpRS)variant P129S | Escherichia coli | tRNATrp | 0.15 | 6.9 × 10⁻⁴ | 220 calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant P129S, tRNATrp |
| tryptophanyl-tRNA synthetase (TrpRS)variant T60R | Escherichia coli | ATPaminoacylation assay with [3H]Trp (filter binding); 4 mM DTT | 0.1 | 0.161 | 0.62 calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant T60R, ATP |
| tryptophanyl-tRNA synthetase (TrpRS)variant T60R | Escherichia coli | ATP20-200 nM TrpRS; tetrasodium pyrophosphate | 12 | 0.073 | 160 calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant T60R, ATP |
| tryptophanyl-tRNA synthetase (TrpRS)variant T60R | Escherichia coli | L-tryptophanaminoacylation assay with [3H]Trp (filter binding); 4 mM DTT | 0.08 | 0.0732 | 1.1 calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant T60R, L-tryptophan |
| tryptophanyl-tRNA synthetase (TrpRS)variant T60R | Escherichia coli | L-tryptophan20-200 nM TrpRS; tetrasodium pyrophosphate | 8.2 | 0.064 | 130 calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant T60R, L-tryptophan |
| tryptophanyl-tRNA synthetase (TrpRS)variant T60R | Escherichia coli | tRNATrp | 0.14 | 4.5 × 10⁻⁴ | 310 calc. | pH 7, 37 °C | PMID 8555191 | Open record: tryptophanyl-tRNA synthetase (TrpRS) variant T60R, tRNATrp |