CatLogenzyme kinetics catalog

Values CatLog read from this paper

Escherichia coli tryptophanyl-tRNA synthetase mutants selected for tryptophan auxotrophy implicate the dimer interface in optimizing amino acid binding

PMID 8555191doi:10.1021/bi952103dOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 43 records (86 values) from this paper: tryptophanyl-tRNA synthetase (TrpRS) from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 43 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 6.1.1.2

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
tryptophanyl-tRNA synthetase (TrpRS)Escherichia coliATPaminoacylation assay with [3H]Trp (filter binding); 4 mM DTT1.50.197.9 calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)Escherichia coliATP20-200 nM TrpRS; tetrasodium pyrophosphate1110.132841 calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)Escherichia coliL-tryptophanaminoacylation assay with [3H]Trp (filter binding); 4 mM DTT20.0124160 calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)Escherichia coliL-tryptophan20-200 nM TrpRS; tetrasodium pyrophosphate1110.01826.10 × 10³ calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)Escherichia colitRNATrp23.4 × 10⁻⁴5.9 × 10³ calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant A133EEscherichia coliATPaminoacylation assay with [3H]Trp (filter binding); 4 mM DTT0.10.1770.56 calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant A133EEscherichia coliATP20-200 nM TrpRS; tetrasodium pyrophosphate400.333120 calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant A133EEscherichia coliL-tryptophanaminoacylation assay with [3H]Trp (filter binding); 4 mM DTT0.60.04812 calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant A133EEscherichia coliL-tryptophan20-200 nM TrpRS; tetrasodium pyrophosphate400.043930 calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant A133EEscherichia colitRNATrp0.265.6 × 10⁻⁴460 calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant D112EEscherichia coliATP2.20.21610 calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant D112EEscherichia coliL-tryptophan2.20.0058380 calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant D112EEscherichia colitRNATrp2.52.8 × 10⁻⁴8.9 × 10³ calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant D112E/P129SEscherichia coliATPaminoacylation assay with [3H]Trp (filter binding); 4 mM DTT0.10.2250.44 calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant D112E/P129SEscherichia coliATP20-200 nM TrpRS; tetrasodium pyrophosphate120.24649 calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant D112E/P129SEscherichia coliL-tryptophanaminoacylation assay with [3H]Trp (filter binding); 4 mM DTT0.120.1141.1 calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant D112E/P129SEscherichia coliL-tryptophan20-200 nM TrpRS; tetrasodium pyrophosphate11.80.18364.5 calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant D112E/P129SEscherichia colitRNATrp0.115.6 × 10⁻⁴200 calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant G329SEscherichia coliATPaminoacylation assay with [3H]Trp (filter binding); 4 mM DTT1.90.17811 calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant G329SEscherichia coliATP20-200 nM TrpRS; tetrasodium pyrophosphate370.083450 calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant G329SEscherichia coliL-tryptophanaminoacylation assay with [3H]Trp (filter binding); 4 mM DTT2.20.21510 calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant G329SEscherichia coliL-tryptophan20-200 nM TrpRS; tetrasodium pyrophosphate600.071850 calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant G329SEscherichia colitRNATrp1.056.5 × 10⁻⁴1.6 × 10³ calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant L91FEscherichia coliATPaminoacylation assay with [3H]Trp (filter binding); 4 mM DTT2.10.20110 calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant L91FEscherichia coliATP20-200 nM TrpRS; tetrasodium pyrophosphate780.194400 calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant L91FEscherichia coliL-tryptophanaminoacylation assay with [3H]Trp (filter binding); 4 mM DTT2.40.07432 calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant L91FEscherichia coliL-tryptophan20-200 nM TrpRS; tetrasodium pyrophosphate750.0461.6 × 10³ calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant L91FEscherichia colitRNATrp2.48.9 × 10⁻⁴2.7 × 10³ calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant M196IEscherichia coliATPaminoacylation assay with [3H]Trp (filter binding); 4 mM DTT0.020.1830.11 calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant M196IEscherichia coliATP20-200 nM TrpRS; tetrasodium pyrophosphate1.80.2986.0 calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant M196IEscherichia coliL-tryptophanaminoacylation assay with [3H]Trp (filter binding); 4 mM DTT0.010.01640.61 calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant M196IEscherichia coliL-tryptophan20-200 nM TrpRS; tetrasodium pyrophosphate1.70.02374 calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant M196IEscherichia colitRNATrp0.022.7 × 10⁻⁴74 calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant P129SEscherichia coliATPaminoacylation assay with [3H]Trp (filter binding); 4 mM DTT0.20.2850.70 calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant P129SEscherichia coliATP20-200 nM TrpRS; tetrasodium pyrophosphate230.28182 calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant P129SEscherichia coliL-tryptophanaminoacylation assay with [3H]Trp (filter binding); 4 mM DTT0.10.0961.0 calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant P129SEscherichia coliL-tryptophan20-200 nM TrpRS; tetrasodium pyrophosphate130.265 calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant P129SEscherichia colitRNATrp0.156.9 × 10⁻⁴220 calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant T60REscherichia coliATPaminoacylation assay with [3H]Trp (filter binding); 4 mM DTT0.10.1610.62 calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant T60REscherichia coliATP20-200 nM TrpRS; tetrasodium pyrophosphate120.073160 calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant T60REscherichia coliL-tryptophanaminoacylation assay with [3H]Trp (filter binding); 4 mM DTT0.080.07321.1 calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant T60REscherichia coliL-tryptophan20-200 nM TrpRS; tetrasodium pyrophosphate8.20.064130 calc.pH 7, 37 °COpen
tryptophanyl-tRNA synthetase (TrpRS)variant T60REscherichia colitRNATrp0.144.5 × 10⁻⁴310 calc.pH 7, 37 °COpen