Values CatLog read from this paper
A high-throughput screen for porphyrin metal chelatases: application to the directed evolution of ferrochelatases for metalloporphyrin biosynthesis
PMID 15300829doi:10.1002/cbic.200400051Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 14 records (21 values) from this paper: ferrochelatase (HemH) from Bacillus subtilis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 7 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| ferrochelatase (HemH) | Bacillus subtilis | ferrous iron (Fe(II)) | — | 0.013 | — | pH 7.6, 30 °C | Open record: ferrochelatase (HemH), ferrous iron (Fe(II)) |
| ferrochelatase (HemH) | Bacillus subtilis | protoporphyrin IX | 0.023 | 9.1 × 10⁻⁴ | 26 calc. | pH 7.6, 30 °C | Open record: ferrochelatase (HemH), protoporphyrin IX |
| ferrochelatase (HemH)variant D76G/K102T (M2) | Bacillus subtilis | ferrous iron (Fe(II)) | — | 0.021 | — | pH 7.6, 30 °C | Open record: ferrochelatase (HemH) variant D76G/K102T (M2), ferrous iron (Fe(II)) |
| ferrochelatase (HemH)variant D76G/K102T (M2) | Bacillus subtilis | protoporphyrin IX | 0.033 | 4.4 × 10⁻⁴ | 76 calc. | pH 7.6, 30 °C | Open record: ferrochelatase (HemH) variant D76G/K102T (M2), protoporphyrin IX |
| ferrochelatase (HemH)variant E61K (M3) | Bacillus subtilis | ferrous iron (Fe(II)) | — | 0.017 | — | pH 7.6, 30 °C | Open record: ferrochelatase (HemH) variant E61K (M3), ferrous iron (Fe(II)) |
| ferrochelatase (HemH)variant E61K (M3) | Bacillus subtilis | protoporphyrin IX | 0.04 | 7.8 × 10⁻⁴ | 51 calc. | pH 7.6, 30 °C | Open record: ferrochelatase (HemH) variant E61K (M3), protoporphyrin IX |
| ferrochelatase (HemH)variant E61K/L185Q/G212D (M6) | Bacillus subtilis | ferrous iron (Fe(II)) | — | 0.014 | — | pH 7.6, 30 °C | Open record: ferrochelatase (HemH) variant E61K/L185Q/G212D (M6), ferrous iron (Fe(II)) |
| ferrochelatase (HemH)variant E61K/L185Q/G212D (M6) | Bacillus subtilis | protoporphyrin IX | 0.055 | 8.6 × 10⁻⁴ | 64 calc. | pH 7.6, 30 °C | Open record: ferrochelatase (HemH) variant E61K/L185Q/G212D (M6), protoporphyrin IX |
| ferrochelatase (HemH)variant M11V/G104A (M4) | Bacillus subtilis | ferrous iron (Fe(II)) | — | 0.0094 | — | pH 7.6, 30 °C | Open record: ferrochelatase (HemH) variant M11V/G104A (M4), ferrous iron (Fe(II)) |
| ferrochelatase (HemH)variant M11V/G104A (M4) | Bacillus subtilis | protoporphyrin IX | 0.038 | 0.0013 | 29 calc. | pH 7.6, 30 °C | Open record: ferrochelatase (HemH) variant M11V/G104A (M4), protoporphyrin IX |
| ferrochelatase (HemH)variant R31G (M5) | Bacillus subtilis | ferrous iron (Fe(II)) | — | 0.01 | — | pH 7.6, 30 °C | Open record: ferrochelatase (HemH) variant R31G (M5), ferrous iron (Fe(II)) |
| ferrochelatase (HemH)variant R31G (M5) | Bacillus subtilis | protoporphyrin IX | 0.02 | 8.3 × 10⁻⁴ | 24 calc. | pH 7.6, 30 °C | Open record: ferrochelatase (HemH) variant R31G (M5), protoporphyrin IX |
| ferrochelatase (HemH)variant T302A (M1) | Bacillus subtilis | ferrous iron (Fe(II)) | — | 0.019 | — | pH 7.6, 30 °C | Open record: ferrochelatase (HemH) variant T302A (M1), ferrous iron (Fe(II)) |
| ferrochelatase (HemH)variant T302A (M1) | Bacillus subtilis | protoporphyrin IX | 0.032 | 7.9 × 10⁻⁴ | 40 calc. | pH 7.6, 30 °C | Open record: ferrochelatase (HemH) variant T302A (M1), protoporphyrin IX |