Values CatLog read from this paper
A reciprocal single mutation affects the metal requirement of 3-deoxy-D-manno-2-octulosonate-8-phosphate (KDO8P) synthases from Aquifex pyrophilus and Escherichia coli
PMID 15308670doi:10.1074/jbc.m404561200Open in Browse
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CatLog read 15 records (30 values) from this paper: 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase) from Escherichia coli, Aquifex pyrophilus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase) | Aquifex pyrophilus | D-arabinose 5-phosphate | — | 0.018 | 330 | pH 7.5, 60 °C | Open record: 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase), D-arabinose 5-phosphate |
| 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase) | Aquifex pyrophilus | phosphoenolpyruvateCd2+-reconstituted enzyme; 100 mM Tris; pH 7.5 | 6 | 0.016 | 370 | pH 7.5, 60 °C | Open record: 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase), phosphoenolpyruvate |
| 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase) | Aquifex pyrophilus | phosphoenolpyruvateMn2+-reconstituted enzyme; 100 mM Tris; pH 7.5 | 9 | — | — | pH 7.5, 60 °C | Open record: 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase), phosphoenolpyruvate |
| 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase) | Aquifex pyrophilus | phosphoenolpyruvateEDTA-treated enzyme; no added metal; 60 °C | 0.045 | — | — | pH 7.5, 60 °C | Open record: 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase), phosphoenolpyruvate |
| 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase) | Aquifex pyrophilus | phosphoenolpyruvaterate vs metal fitted to Michaelis-Menten; 60 °C | 8.5 | — | — | pH 7.5, 60 °C | Open record: 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase), phosphoenolpyruvate |
| 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase)variant C11N | Aquifex pyrophilus | phosphoenolpyruvateEDTA-treated enzyme; no added metal; 100 mM Tris; pH 7.5 | 0.42 | — | — | pH 7.5, 60 °C | Open record: 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase) variant C11N, phosphoenolpyruvate |
| 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase)variant C11N | Aquifex pyrophilus | phosphoenolpyruvaterate vs metal fitted to Michaelis-Menten; 60 °C | 1.5 | — | — | pH 7.5, 60 °C | Open record: 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase) variant C11N, phosphoenolpyruvate |
| 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase) | Escherichia coli | D-arabinose 5-phosphate | — | 0.02 | 300 | pH 7.5, 37 °C | Open record: 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase), D-arabinose 5-phosphate |
| 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase) | Escherichia coli | phosphoenolpyruvate | 6.1 | 0.006 | 1 × 10³ | pH 7.5, 37 °C | Open record: 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase), phosphoenolpyruvate |
| 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase)variant N26C | Escherichia coli | D-arabinose 5-phosphateEDTA-treated enzyme; no added metal | — | 0.075 | 5 | pH 7.5, 37 °C | Open record: 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase) variant N26C, D-arabinose 5-phosphate |
| 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase)variant N26C | Escherichia coli | D-arabinose 5-phosphateMn2+-reconstituted enzyme | — | 0.07 | 30 | pH 7.5, 37 °C | Open record: 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase) variant N26C, D-arabinose 5-phosphate |
| 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase)variant N26C | Escherichia coli | D-arabinose 5-phosphateCd2+-reconstituted enzyme | — | 0.11 | 20 | pH 7.5, 37 °C | Open record: 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase) variant N26C, D-arabinose 5-phosphate |
| 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase)variant N26C | Escherichia coli | phosphoenolpyruvateEDTA-treated enzyme; no added metal | 0.36 | 0.032 | 10 | pH 7.5, 37 °C | Open record: 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase) variant N26C, phosphoenolpyruvate |
| 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase)variant N26C | Escherichia coli | phosphoenolpyruvateMn2+-reconstituted enzyme | 1.9 | 0.019 | 100 | pH 7.5, 37 °C | Open record: 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase) variant N26C, phosphoenolpyruvate |
| 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase)variant N26C | Escherichia coli | phosphoenolpyruvateCd2+-reconstituted enzyme | 1.9 | 0.0058 | 330 | pH 7.5, 37 °C | Open record: 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase) variant N26C, phosphoenolpyruvate |