CatLogenzyme kinetics catalog

EC 4.1.2.16: 2-dehydro-3-deoxy-phosphooctonate aldolase

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CatLog read 35 records for EC 4.1.2.16 from 3 papers, for 4 organisms. Names in these papers: 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase), 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS), KDO-8-P synthase.

EC 4.1.2.16 is no longer current; BRENDA: “transferred to EC 2.5.1.55”.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

Papers

PaperOrganismsRecords
A reciprocal single mutation affects the metal requirement of 3-deoxy-D-manno-2-octulosonate-8-phosphate (KDO8P) synthases from Aquifex pyrophilus and Escherichia coliAquifex pyrophilus, Escherichia coli15
Electronic structure of the metal center in the Cd(2+), Zn(2+), and Cu(2+) substituted forms of KDO8P synthase: implications for catalysisAquifex aeolicus14
Helicobacter pylori 3-deoxy-D-manno-octulosonate-8-phosphate (KDO-8-P) synthase is a zinc-metalloenzymeEscherichia coli, Helicobacter pylori6

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase)Aquifex pyrophilusD-arabinose 5-phosphate—0.018330pH 7.5, 60 °CPMID 15308670Open
3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase)Aquifex pyrophilusphosphoenolpyruvateCd2+-reconstituted enzyme; 100 mM Tris; pH 7.560.016370pH 7.5, 60 °CPMID 15308670Open
3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase)Aquifex pyrophilusphosphoenolpyruvateMn2+-reconstituted enzyme; 100 mM Tris; pH 7.59——pH 7.5, 60 °CPMID 15308670Open
3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase)Aquifex pyrophilusphosphoenolpyruvateEDTA-treated enzyme; no added metal; 60 °C0.045——pH 7.5, 60 °CPMID 15308670Open
3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase)Aquifex pyrophilusphosphoenolpyruvaterate vs metal fitted to Michaelis-Menten; 60 °C8.5——pH 7.5, 60 °CPMID 15308670Open
3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase)variant C11NAquifex pyrophilusphosphoenolpyruvateEDTA-treated enzyme; no added metal; 100 mM Tris; pH 7.50.42——pH 7.5, 60 °CPMID 15308670Open
3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase)variant C11NAquifex pyrophilusphosphoenolpyruvaterate vs metal fitted to Michaelis-Menten; 60 °C1.5——pH 7.5, 60 °CPMID 15308670Open
3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase)Escherichia coliD-arabinose 5-phosphate—0.02300pH 7.5, 37 °CPMID 15308670Open
3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase)Escherichia coliphosphoenolpyruvate6.10.0061 × 10³pH 7.5, 37 °CPMID 15308670Open
3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase)variant N26CEscherichia coliD-arabinose 5-phosphateEDTA-treated enzyme; no added metal—0.0755pH 7.5, 37 °CPMID 15308670Open
3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase)variant N26CEscherichia coliD-arabinose 5-phosphateMn2+-reconstituted enzyme—0.0730pH 7.5, 37 °CPMID 15308670Open
3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase)variant N26CEscherichia coliD-arabinose 5-phosphateCd2+-reconstituted enzyme—0.1120pH 7.5, 37 °CPMID 15308670Open
3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase)variant N26CEscherichia coliphosphoenolpyruvateEDTA-treated enzyme; no added metal0.360.03210pH 7.5, 37 °CPMID 15308670Open
3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase)variant N26CEscherichia coliphosphoenolpyruvateMn2+-reconstituted enzyme1.90.019100pH 7.5, 37 °CPMID 15308670Open
3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8P synthase)variant N26CEscherichia coliphosphoenolpyruvateCd2+-reconstituted enzyme1.90.0058330pH 7.5, 37 °CPMID 15308670Open
3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS)Aquifex aeolicusarabinose 5-phosphateCd2+-substituted enzyme; 50 µM CdCl2 in the reaction mixture—0.00318—pH 7.5, 40 °CPMID 19228070Open
3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS)Aquifex aeolicusarabinose 5-phosphateCd2+-substituted enzyme; Prep 1—0.002—pH 7.5, 40 °CPMID 19228070Open
3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS)Aquifex aeolicusarabinose 5-phosphateZn2+-substituted enzyme; Prep 1—5 × 10⁻⁴—pH 7.5, 40 °CPMID 19228070Open
3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS)Aquifex aeolicusarabinose 5-phosphateCu2+-substituted enzyme; Prep 1—0.00156—pH 7.5, 40 °CPMID 19228070Open
3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS)Aquifex aeolicusarabinose 5-phosphatePrep 1—0.00123—pH 7.5, 40 °CPMID 19228070Open
3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS)Aquifex aeolicusarabinose 5-phosphateCd2+-substituted enzyme; 50 µM CdCl2 in the reaction mixture—0.00227—pH 5, 40 °CPMID 19228070Open
3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS)Aquifex aeolicusarabinose 5-phosphateCu2+-substituted enzyme; Prep 1—0.01926—pH 5, 40 °CPMID 19228070Open
3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS)Aquifex aeolicusphosphoenolpyruvateCd2+-substituted enzyme; 50 µM CdCl2 in the reaction mixture0.481.6 × 10⁻⁴3.0 × 10³ calc.pH 7.5, 40 °CPMID 19228070Open
3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS)Aquifex aeolicusphosphoenolpyruvateCd2+-substituted enzyme; Prep 10.411.4 × 10⁻⁴2.9 × 10³ calc.pH 7.5, 40 °CPMID 19228070Open
3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS)Aquifex aeolicusphosphoenolpyruvateZn2+-substituted enzyme; Prep 10.472.8 × 10⁻⁴1.7 × 10³ calc.pH 7.5, 40 °CPMID 19228070Open
3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS)Aquifex aeolicusphosphoenolpyruvateCu2+-substituted enzyme; Prep 10.341.5 × 10⁻⁴2.3 × 10³ calc.pH 7.5, 40 °CPMID 19228070Open
3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS)Aquifex aeolicusphosphoenolpyruvatePrep 10.311.6 × 10⁻⁴1.9 × 10³ calc.pH 7.5, 40 °CPMID 19228070Open
3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS)Aquifex aeolicusphosphoenolpyruvateCd2+-substituted enzyme; 50 µM CdCl2 in the reaction mixture0.464 × 10⁻⁵1.2 × 10⁴ calc.pH 5, 40 °CPMID 19228070Open
3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS)Aquifex aeolicusphosphoenolpyruvateCu2+-substituted enzyme; Prep 10.366.6 × 10⁻⁶5.5 × 10⁴ calc.pH 5, 40 °CPMID 19228070Open
KDO-8-P synthaseEscherichia coliD-arabinose 5-phosphate5.3080.00773687 calc.pH 6.5PMID 11904225Open
KDO-8-P synthaseEscherichia coliphosphoenolpyruvate—0.00443—pH 6.5PMID 11904225Open
KDO-8-P synthaseHelicobacter pyloriD-arabinose 5-phosphateZn2+-holoenzyme; Zn2+-holoenzyme: recombinant0.490.038513 calc.pH 6.5PMID 11904225Open
KDO-8-P synthaseHelicobacter pyloriD-arabinose 5-phosphateCd2+-holoenzyme1.0850.00593183 calc.pH 6.5PMID 11904225Open
KDO-8-P synthaseHelicobacter pyloriphosphoenolpyruvateZn2+-holoenzyme; Zn2+-holoenzyme: recombinant—0.0075—pH 6.5PMID 11904225Open
KDO-8-P synthaseHelicobacter pyloriphosphoenolpyruvateCd2+-holoenzyme—0.00648—pH 6.5PMID 11904225Open