Values CatLog read from this paper
Electronic structure of the metal center in the Cd(2+), Zn(2+), and Cu(2+) substituted forms of KDO8P synthase: implications for catalysis
PMID 19228070doi:10.1021/bi801955hOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 14 records (21 values) from this paper: 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS) from Aquifex aeolicus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 7 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS) | Aquifex aeolicus | arabinose 5-phosphateCd2+-substituted enzyme; 50 µM CdCl2 in the reaction mixture | — | 0.00318 | — | pH 7.5, 40 °C | Open record: 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS), arabinose 5-phosphate |
| 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS) | Aquifex aeolicus | arabinose 5-phosphateCd2+-substituted enzyme; Prep 1 | — | 0.002 | — | pH 7.5, 40 °C | Open record: 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS), arabinose 5-phosphate |
| 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS) | Aquifex aeolicus | arabinose 5-phosphateZn2+-substituted enzyme; Prep 1 | — | 5 × 10⁻⁴ | — | pH 7.5, 40 °C | Open record: 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS), arabinose 5-phosphate |
| 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS) | Aquifex aeolicus | arabinose 5-phosphateCu2+-substituted enzyme; Prep 1 | — | 0.00156 | — | pH 7.5, 40 °C | Open record: 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS), arabinose 5-phosphate |
| 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS) | Aquifex aeolicus | arabinose 5-phosphatePrep 1 | — | 0.00123 | — | pH 7.5, 40 °C | Open record: 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS), arabinose 5-phosphate |
| 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS) | Aquifex aeolicus | arabinose 5-phosphateCd2+-substituted enzyme; 50 µM CdCl2 in the reaction mixture | — | 0.00227 | — | pH 5, 40 °C | Open record: 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS), arabinose 5-phosphate |
| 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS) | Aquifex aeolicus | arabinose 5-phosphateCu2+-substituted enzyme; Prep 1 | — | 0.01926 | — | pH 5, 40 °C | Open record: 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS), arabinose 5-phosphate |
| 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS) | Aquifex aeolicus | phosphoenolpyruvateCd2+-substituted enzyme; 50 µM CdCl2 in the reaction mixture | 0.48 | 1.6 × 10⁻⁴ | 3.0 × 10³ calc. | pH 7.5, 40 °C | Open record: 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS), phosphoenolpyruvate |
| 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS) | Aquifex aeolicus | phosphoenolpyruvateCd2+-substituted enzyme; Prep 1 | 0.41 | 1.4 × 10⁻⁴ | 2.9 × 10³ calc. | pH 7.5, 40 °C | Open record: 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS), phosphoenolpyruvate |
| 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS) | Aquifex aeolicus | phosphoenolpyruvateZn2+-substituted enzyme; Prep 1 | 0.47 | 2.8 × 10⁻⁴ | 1.7 × 10³ calc. | pH 7.5, 40 °C | Open record: 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS), phosphoenolpyruvate |
| 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS) | Aquifex aeolicus | phosphoenolpyruvateCu2+-substituted enzyme; Prep 1 | 0.34 | 1.5 × 10⁻⁴ | 2.3 × 10³ calc. | pH 7.5, 40 °C | Open record: 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS), phosphoenolpyruvate |
| 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS) | Aquifex aeolicus | phosphoenolpyruvatePrep 1 | 0.31 | 1.6 × 10⁻⁴ | 1.9 × 10³ calc. | pH 7.5, 40 °C | Open record: 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS), phosphoenolpyruvate |
| 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS) | Aquifex aeolicus | phosphoenolpyruvateCd2+-substituted enzyme; 50 µM CdCl2 in the reaction mixture | 0.46 | 4 × 10⁻⁵ | 1.2 × 10⁴ calc. | pH 5, 40 °C | Open record: 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS), phosphoenolpyruvate |
| 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS) | Aquifex aeolicus | phosphoenolpyruvateCu2+-substituted enzyme; Prep 1 | 0.36 | 6.6 × 10⁻⁶ | 5.5 × 10⁴ calc. | pH 5, 40 °C | Open record: 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS), phosphoenolpyruvate |