CatLogenzyme kinetics catalog

Values CatLog read from this paper

The coenzyme specificity of Candida tenuis xylose reductase (AKR2B5) explored by site-directed mutagenesis and X-ray crystallography

PMID 15320875doi:10.1042/bj20040363Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 36 records (54 values) from this paper: xylose reductase (CtXR, AKR2B5) from Candida tenuis.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 18 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.1.1.21

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
xylose reductase (CtXR, AKR2B5)Candida tenuisD-xylosecosubstrate NADH111420.077 calc.pH 7, 25 °COpen
xylose reductase (CtXR, AKR2B5)Candida tenuisD-xylosecosubstrate NADPH13960.14 calc.pH 7, 25 °COpen
xylose reductase (CtXR, AKR2B5)Candida tenuisNADH—0.038—pH 7, 25 °COpen
xylose reductase (CtXR, AKR2B5)Candida tenuisNADPH—0.003—pH 7, 25 °COpen
xylose reductase (CtXR, AKR2B5)variant K274ECandida tenuisD-xylosecosubstrate NADH0.075401.3 × 10⁻⁴ calc.pH 7, 25 °COpen
xylose reductase (CtXR, AKR2B5)variant K274ECandida tenuisD-xylosecosubstrate NADPH0.182.1 × 10³8.6 × 10⁻⁵ calc.pH 7, 25 °COpen
xylose reductase (CtXR, AKR2B5)variant K274ECandida tenuisNADH——0.133pH 7, 25 °COpen
xylose reductase (CtXR, AKR2B5)variant K274ECandida tenuisNADPH——0.47pH 7, 25 °COpen
xylose reductase (CtXR, AKR2B5)variant K274GCandida tenuisD-xylosecosubstrate NADH155590.027 calc.pH 7, 25 °COpen
xylose reductase (CtXR, AKR2B5)variant K274GCandida tenuisD-xylosecosubstrate NADPH107220.014 calc.pH 7, 25 °COpen
xylose reductase (CtXR, AKR2B5)variant K274GCandida tenuisNADH—0.146—pH 7, 25 °COpen
xylose reductase (CtXR, AKR2B5)variant K274GCandida tenuisNADPH—0.032—pH 7, 25 °COpen
xylose reductase (CtXR, AKR2B5)variant K274MCandida tenuisD-xylosecosubstrate NADH192290.083 calc.pH 7, 25 °COpen
xylose reductase (CtXR, AKR2B5)variant K274MCandida tenuisD-xylosecosubstrate NADPH365060.071 calc.pH 7, 25 °COpen
xylose reductase (CtXR, AKR2B5)variant K274MCandida tenuisNADH—0.38—pH 7, 25 °COpen
xylose reductase (CtXR, AKR2B5)variant K274MCandida tenuisNADPH—0.075—pH 7, 25 °COpen
xylose reductase (CtXR, AKR2B5)variant K274RCandida tenuisD-xylosecosubstrate NADH8540.15 calc.pH 7, 25 °COpen
xylose reductase (CtXR, AKR2B5)variant K274RCandida tenuisD-xylosecosubstrate NADPH263500.074 calc.pH 7, 25 °COpen
xylose reductase (CtXR, AKR2B5)variant K274RCandida tenuisNADH—0.021—pH 7, 25 °COpen
xylose reductase (CtXR, AKR2B5)variant K274RCandida tenuisNADPH—0.005—pH 7, 25 °COpen
xylose reductase (CtXR, AKR2B5)variant K274R/N276DCandida tenuisD-xylosecosubstrate NADH121060.11 calc.pH 7, 25 °COpen
xylose reductase (CtXR, AKR2B5)variant K274R/N276DCandida tenuisD-xylosecosubstrate NADPH307220.042 calc.pH 7, 25 °COpen
xylose reductase (CtXR, AKR2B5)variant K274R/N276DCandida tenuisNADH—0.041—pH 7, 25 °COpen
xylose reductase (CtXR, AKR2B5)variant K274R/N276DCandida tenuisNADPH—0.128—pH 7, 25 °COpen
xylose reductase (CtXR, AKR2B5)variant N276DCandida tenuisD-xylosecosubstrate NADH14990.14 calc.pH 7, 25 °COpen
xylose reductase (CtXR, AKR2B5)variant N276DCandida tenuisD-xylosecosubstrate NADPH371700.22 calc.pH 7, 25 °COpen
xylose reductase (CtXR, AKR2B5)variant N276DCandida tenuisNADH—0.026—pH 7, 25 °COpen
xylose reductase (CtXR, AKR2B5)variant N276DCandida tenuisNADPH—0.017—pH 7, 25 °COpen
xylose reductase (CtXR, AKR2B5)variant R280HCandida tenuisD-xylosecosubstrate NADH21940.22 calc.pH 7, 25 °COpen
xylose reductase (CtXR, AKR2B5)variant R280HCandida tenuisD-xylosecosubstrate NADPH151070.14 calc.pH 7, 25 °COpen
xylose reductase (CtXR, AKR2B5)variant R280HCandida tenuisNADH—0.015—pH 7, 25 °COpen
xylose reductase (CtXR, AKR2B5)variant R280HCandida tenuisNADPH—0.004—pH 7, 25 °COpen
xylose reductase (CtXR, AKR2B5)variant S275ACandida tenuisD-xylosecosubstrate NADH244380.055 calc.pH 7, 25 °COpen
xylose reductase (CtXR, AKR2B5)variant S275ACandida tenuisD-xylosecosubstrate NADPH291150.25 calc.pH 7, 25 °COpen
xylose reductase (CtXR, AKR2B5)variant S275ACandida tenuisNADH—0.075—pH 7, 25 °COpen
xylose reductase (CtXR, AKR2B5)variant S275ACandida tenuisNADPH—0.009—pH 7, 25 °COpen