CatLogenzyme kinetics catalog

Values CatLog read from this paper

Identification of residues Asn89, Ile90, and Val107 of the factor IXa second epidermal growth factor domain that are essential for the assembly of the factor X-activating complex on activated…

PMID 15328360doi:10.1074/jbc.m406552200Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 15 records (23 values) from this paper: coagulation factor IXa (FIXa) from Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 8 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 3.4.21.22

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
coagulation factor IXa (FIXa)Homo sapienscoagulation factor XSFLLRN-activated platelets (5 x 10^7/ml) as the surface3.44.28 × 10⁻⁵7.9 × 10⁴ calc.pH 7.4, 37 °COpen
coagulation factor IXa (FIXa)Homo sapienscoagulation factor Xextruded l-alpha-dioleoyl-PC:PS (3:1) vesicles; total 2 µM—3.96 × 10⁻⁵—pH 7.4, 37 °COpen
coagulation factor IXa (FIXa)variant D104AHomo sapienscoagulation factor XSFLLRN-activated platelets (5 x 10^7/ml) as the surface2.92 × 10⁻⁵1.4 × 10⁵ calc.pH 7.4, 37 °COpen
coagulation factor IXa (FIXa)variant D104AHomo sapienscoagulation factor Xextruded l-alpha-dioleoyl-PC:PS (3:1) vesicles; total 2 µM—7.87 × 10⁻⁵—pH 7.4, 37 °COpen
coagulation factor IXa (FIXa)variant I90AHomo sapienscoagulation factor XSFLLRN-activated platelets (5 x 10^7/ml) as the surface4.16.8 × 10⁻⁶6.0 × 10⁵ calc.pH 7.4, 37 °COpen
coagulation factor IXa (FIXa)variant I90AHomo sapienscoagulation factor Xextruded l-alpha-dioleoyl-PC:PS (3:1) vesicles; total 2 µM—1.81 × 10⁻⁵—pH 7.4, 37 °COpen
coagulation factor IXa (FIXa)variant K91AHomo sapienscoagulation factor XSFLLRN-activated platelets (5 x 10^7/ml) as the surface5.22.95 × 10⁻⁵1.8 × 10⁵ calc.pH 7.4, 37 °COpen
coagulation factor IXa (FIXa)variant K91AHomo sapienscoagulation factor Xextruded l-alpha-dioleoyl-PC:PS (3:1) vesicles; total 2 µM—6.29 × 10⁻⁵—pH 7.4, 37 °COpen
coagulation factor IXa (FIXa)variant N105AHomo sapienscoagulation factor XSFLLRN-activated platelets (5 x 10^7/ml) as the surface3.82.51 × 10⁻⁵1.5 × 10⁵ calc.pH 7.4, 37 °COpen
coagulation factor IXa (FIXa)variant N105AHomo sapienscoagulation factor Xextruded l-alpha-dioleoyl-PC:PS (3:1) vesicles; total 2 µM—8.69 × 10⁻⁵—pH 7.4, 37 °COpen
coagulation factor IXa (FIXa)variant N89AHomo sapienscoagulation factor X16.61.542 × 10⁻⁴1.08 × 10⁵ calc.pH 7.4, 37 °COpen
coagulation factor IXa (FIXa)variant R94AHomo sapienscoagulation factor XSFLLRN-activated platelets (5 x 10^7/ml) as the surface2.92.24 × 10⁻⁵1.3 × 10⁵ calc.pH 7.4, 37 °COpen
coagulation factor IXa (FIXa)variant R94AHomo sapienscoagulation factor Xextruded l-alpha-dioleoyl-PC:PS (3:1) vesicles; total 2 µM—6.4 × 10⁻⁵—pH 7.4, 37 °COpen
coagulation factor IXa (FIXa)variant V107AHomo sapienscoagulation factor XSFLLRN-activated platelets (5 x 10^7/ml) as the surface3.38.9 × 10⁻⁶3.7 × 10⁵ calc.pH 7.4, 37 °COpen
coagulation factor IXa (FIXa)variant V107AHomo sapienscoagulation factor Xextruded l-alpha-dioleoyl-PC:PS (3:1) vesicles; total 2 µM—3.4 × 10⁻⁵—pH 7.4, 37 °COpen