CatLogenzyme kinetics catalog

EC 3.4.21.22: coagulation factor IXa

HydrolasesOpen in Browse

CatLog read 59 records for EC 3.4.21.22 from 4 papers, for 2 organisms. Names in these papers: activated coagulation factor IX (FIXa), factor IXa (FIXa), coagulation factor IXa (FIXa), factor IXa.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 23 records for EC 3.4.21.22 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.4.21.22 in Browse.

Papers

PaperOrganismsRecords
Factor IX mutants with enhanced catalytic activityHomo sapiens18
Na+ site in blood coagulation factor IXa: effect on catalysis and factor VIIIa bindingHomo sapiens16
Identification of residues Asn89, Ile90, and Val107 of the factor IXa second epidermal growth factor domain that are essential for the assembly of the factor X-activating complex on activated…Homo sapiens15
Active-site mapping of bovine and human blood coagulation serine proteases using synthetic peptide 4-nitroanilide and thio ester substratesBos taurus10

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
activated coagulation factor IX (FIXa)Homo sapiensH-D-Leu-Ph'Gly-Arg-pNA.2AcOHplasma-derived FIX activated with FXIa——1.05 × 10⁻⁴37 °CPMID 19656280Open
activated coagulation factor IX (FIXa)Homo sapiensH-D-Leu-Ph'Gly-Arg-pNA.2AcOH——1.47 × 10⁻⁴37 °CPMID 19656280Open
activated coagulation factor IX (FIXa)Homo sapienshuman coagulation factor XFX activation without FVIIIa in 25 mM Hepes; 6 nM FIXa2.12 × 10⁻⁴6.5 × 10⁻⁵3.2pH 7.35, 37 °CPMID 19656280Open
activated coagulation factor IX (FIXa)Homo sapienshuman coagulation factor XFX activation with FVIIIa in 25 mM Hepes2.181.3 × 10⁻⁵1.7 × 10⁵pH 7.35, 37 °CPMID 19656280Open
activated coagulation factor IX (FIXa)Homo sapienshuman coagulation factor XFX activation without FVIIIa in 25 mM Hepes; 6 nM FIXa2.87 × 10⁻⁴6.8 × 10⁻⁵4.2pH 7.35, 37 °CPMID 19656280Open
activated coagulation factor IX (FIXa)Homo sapienshuman coagulation factor XFX activation with FVIIIa in 25 mM Hepes1.71.1 × 10⁻⁵1.5 × 10⁵pH 7.35, 37 °CPMID 19656280Open
activated coagulation factor IX (FIXa)variant Y94F/A95aK/K98T/Y177F/I213V/E219G (FIXa-M)Homo sapiensH-D-Leu-Ph'Gly-Arg-pNA.2AcOH——1.23 × 10⁻⁴37 °CPMID 19656280Open
activated coagulation factor IX (FIXa)variant Y94F/A95aK/K98T/Y177F/I213V/E219G (FIXa-M)Homo sapienshuman coagulation factor XFX activation without FVIIIa in 25 mM Hepes; 6 nM FIXa0.0010085.5 × 10⁻⁵18.2pH 7.35, 37 °CPMID 19656280Open
activated coagulation factor IX (FIXa)variant Y94F/A95aK/K98T/Y177F/I213V/E219G (FIXa-M)Homo sapienshuman coagulation factor XFX activation with FVIIIa in 25 mM Hepes2.031.3 × 10⁻⁵1.5 × 10⁵pH 7.35, 37 °CPMID 19656280Open
activated coagulation factor IX (FIXa)variant Y94F/K98T (FIXa-C)Homo sapiensH-D-Leu-Ph'Gly-Arg-pNA.2AcOH——3.7 × 10⁻⁴37 °CPMID 19656280Open
activated coagulation factor IX (FIXa)variant Y94F/K98T (FIXa-C)Homo sapienshuman coagulation factor XFX activation without FVIIIa in 25 mM Hepes; 6 nM FIXa5.87 × 10⁻⁴8.3 × 10⁻⁵7.2pH 7.35, 37 °CPMID 19656280Open
activated coagulation factor IX (FIXa)variant Y94F/K98T (FIXa-C)Homo sapienshuman coagulation factor XFX activation with FVIIIa in 25 mM Hepes31.5 × 10⁻⁵2 × 10⁵pH 7.35, 37 °CPMID 19656280Open
activated coagulation factor IX (FIXa)variant Y94F/K98T/Y177F (FIXa-I)Homo sapiensH-D-Leu-Ph'Gly-Arg-pNA.2AcOH——1.05 × 10⁻⁴37 °CPMID 19656280Open
activated coagulation factor IX (FIXa)variant Y94F/K98T/Y177F (FIXa-I)Homo sapienshuman coagulation factor XFX activation without FVIIIa in 25 mM Hepes; 6 nM FIXa3.3 × 10⁻⁴6.8 × 10⁻⁵4.8pH 7.35, 37 °CPMID 19656280Open
activated coagulation factor IX (FIXa)variant Y94F/K98T/Y177F (FIXa-I)Homo sapienshuman coagulation factor XFX activation with FVIIIa in 25 mM Hepes1.131.4 × 10⁻⁵8.3 × 10⁴pH 7.35, 37 °CPMID 19656280Open
activated coagulation factor IX (FIXa)variant Y94F/K98T/Y177F/I213V/E219G (FIXa-L)Homo sapiensH-D-Leu-Ph'Gly-Arg-pNA.2AcOH——2.53 × 10⁻⁴37 °CPMID 19656280Open
activated coagulation factor IX (FIXa)variant Y94F/K98T/Y177F/I213V/E219G (FIXa-L)Homo sapienshuman coagulation factor XFX activation without FVIIIa in 25 mM Hepes; 6 nM FIXa0.002224 × 10⁻⁵55pH 7.35, 37 °CPMID 19656280Open
activated coagulation factor IX (FIXa)variant Y94F/K98T/Y177F/I213V/E219G (FIXa-L)Homo sapienshuman coagulation factor XFX activation with FVIIIa in 25 mM Hepes8.81.7 × 10⁻⁵5.3 × 10⁵pH 7.35, 37 °CPMID 19656280Open
coagulation factor IXa (FIXa)Homo sapienscoagulation factor XSFLLRN-activated platelets (5 x 10^7/ml) as the surface3.44.28 × 10⁻⁵7.9 × 10⁴ calc.pH 7.4, 37 °CPMID 15328360Open
coagulation factor IXa (FIXa)Homo sapienscoagulation factor Xextruded l-alpha-dioleoyl-PC:PS (3:1) vesicles; total 2 µM—3.96 × 10⁻⁵—pH 7.4, 37 °CPMID 15328360Open
coagulation factor IXa (FIXa)variant D104AHomo sapienscoagulation factor XSFLLRN-activated platelets (5 x 10^7/ml) as the surface2.92 × 10⁻⁵1.4 × 10⁵ calc.pH 7.4, 37 °CPMID 15328360Open
coagulation factor IXa (FIXa)variant D104AHomo sapienscoagulation factor Xextruded l-alpha-dioleoyl-PC:PS (3:1) vesicles; total 2 µM—7.87 × 10⁻⁵—pH 7.4, 37 °CPMID 15328360Open
coagulation factor IXa (FIXa)variant I90AHomo sapienscoagulation factor XSFLLRN-activated platelets (5 x 10^7/ml) as the surface4.16.8 × 10⁻⁶6.0 × 10⁵ calc.pH 7.4, 37 °CPMID 15328360Open
coagulation factor IXa (FIXa)variant I90AHomo sapienscoagulation factor Xextruded l-alpha-dioleoyl-PC:PS (3:1) vesicles; total 2 µM—1.81 × 10⁻⁵—pH 7.4, 37 °CPMID 15328360Open
coagulation factor IXa (FIXa)variant K91AHomo sapienscoagulation factor XSFLLRN-activated platelets (5 x 10^7/ml) as the surface5.22.95 × 10⁻⁵1.8 × 10⁵ calc.pH 7.4, 37 °CPMID 15328360Open
coagulation factor IXa (FIXa)variant K91AHomo sapienscoagulation factor Xextruded l-alpha-dioleoyl-PC:PS (3:1) vesicles; total 2 µM—6.29 × 10⁻⁵—pH 7.4, 37 °CPMID 15328360Open
coagulation factor IXa (FIXa)variant N105AHomo sapienscoagulation factor XSFLLRN-activated platelets (5 x 10^7/ml) as the surface3.82.51 × 10⁻⁵1.5 × 10⁵ calc.pH 7.4, 37 °CPMID 15328360Open
coagulation factor IXa (FIXa)variant N105AHomo sapienscoagulation factor Xextruded l-alpha-dioleoyl-PC:PS (3:1) vesicles; total 2 µM—8.69 × 10⁻⁵—pH 7.4, 37 °CPMID 15328360Open
coagulation factor IXa (FIXa)variant N89AHomo sapienscoagulation factor X16.61.542 × 10⁻⁴1.08 × 10⁵ calc.pH 7.4, 37 °CPMID 15328360Open
coagulation factor IXa (FIXa)variant R94AHomo sapienscoagulation factor XSFLLRN-activated platelets (5 x 10^7/ml) as the surface2.92.24 × 10⁻⁵1.3 × 10⁵ calc.pH 7.4, 37 °CPMID 15328360Open
coagulation factor IXa (FIXa)variant R94AHomo sapienscoagulation factor Xextruded l-alpha-dioleoyl-PC:PS (3:1) vesicles; total 2 µM—6.4 × 10⁻⁵—pH 7.4, 37 °CPMID 15328360Open
coagulation factor IXa (FIXa)variant V107AHomo sapienscoagulation factor XSFLLRN-activated platelets (5 x 10^7/ml) as the surface3.38.9 × 10⁻⁶3.7 × 10⁵ calc.pH 7.4, 37 °CPMID 15328360Open
coagulation factor IXa (FIXa)variant V107AHomo sapienscoagulation factor Xextruded l-alpha-dioleoyl-PC:PS (3:1) vesicles; total 2 µM—3.4 × 10⁻⁵—pH 7.4, 37 °CPMID 15328360Open
factor IXaBos taurusN-benzyloxycarbonyl-L-alanyl-glycyl-L-arginine 4-nitroanilide——0.015pH 7.5, 25 °CPMID 6370301Open
factor IXaBos taurusN-benzyloxycarbonyl-L-alpha-glutamyl-glycyl-L-arginine 4-nitroanilide——0.503pH 7.5, 25 °CPMID 6370301Open
factor IXaBos taurusN-benzyloxycarbonyl-L-alpha-glutamyl-L-phenylalanyl-L-arginine 4-nitroanilide——0.0019pH 7.5, 25 °CPMID 6370301Open
factor IXaBos taurusN-benzyloxycarbonyl-L-asparaginyl-glycyl-L-arginine 4-nitroanilide——0.011pH 7.5, 25 °CPMID 6370301Open
factor IXaBos taurusN-benzyloxycarbonyl-L-asparaginyl-L-phenylalanyl-L-arginine 4-nitroanilide——0.0079pH 7.5, 25 °CPMID 6370301Open
factor IXaBos taurusN-benzyloxycarbonyl-L-lysyl-glycyl-L-arginine 4-nitroanilide——0.0064pH 7.5, 25 °CPMID 6370301Open
factor IXaBos taurusN-benzyloxycarbonyl-L-lysyl-L-phenylalanyl-L-arginine 4-nitroanilide——0.0037pH 7.5, 25 °CPMID 6370301Open
factor IXaBos taurusN-benzyloxycarbonyl-L-prolyl-glycyl-L-arginine 4-nitroanilide——7 × 10⁻⁵pH 7.5, 25 °CPMID 6370301Open
factor IXaBos taurusN-benzyloxycarbonyl-L-seryl-glycyl-L-arginine 4-nitroanilide——0.0017pH 7.5, 25 °CPMID 6370301Open
factor IXaBos taurusN-benzyloxycarbonyl-L-seryl-L-phenylalanyl-L-arginine 4-nitroanilide——8.5 × 10⁻⁴pH 7.5, 25 °CPMID 6370301Open
factor IXa (FIXa)Homo sapiensCH3SO2-D-Leu-Gly-Arg-p-nitroanilide50 mM Tris-HCl; 0.1% (w/v) PEG 8000; Rb+ as compensating ion0.432——pH 7.4PMID 15913649Open
factor IXa (FIXa)Homo sapiensCH3SO2-D-Leu-Gly-Arg-p-nitroanilide50 mM Tris-HCl; 0.1% (w/v) PEG 8000; Rb+ as compensating ion0.562——pH 7.4PMID 15913649Open
factor IXa (FIXa)Homo sapiensCH3SO2-D-Leu-Gly-Arg-p-nitroanilide50 mM Tris-HCl; 0.1% (w/v) PEG 8000; Rb+ as compensating ion3.58——pH 7.4PMID 15913649Open
factor IXa (FIXa)Homo sapiensCH3SO2-D-Leu-Gly-Arg-p-nitroanilide50 mM Tris-HCl; 0.1% (w/v) PEG 8000; Rb+ as compensating ion3.53——pH 7.4PMID 15913649Open
factor IXa (FIXa)Homo sapiensCH3SO2-D-Leu-Gly-Arg-p-nitroanilide50 mM Tris (pH 7.4); 0.1% PEG 8000; 1 mM EDTA; no Na+0.421.90.22pH 7.4PMID 15913649Open
factor IXa (FIXa)Homo sapiensCH3SO2-D-Leu-Gly-Arg-p-nitroanilide50 mM Tris (pH 7.4); 0.1% PEG 8000; 1 mM EDTA; 200 mM Na+0.630.51.22pH 7.4PMID 15913649Open
factor IXa (FIXa)Homo sapiensCH3SO2-D-Leu-Gly-Arg-p-nitroanilide50 mM Tris (pH 7.4); 0.1% PEG 8000; 5 mM CaCl2; no Na+3.158.70.37pH 7.4PMID 15913649Open
factor IXa (FIXa)Homo sapiensCH3SO2-D-Leu-Gly-Arg-p-nitroanilide50 mM Tris (pH 7.4); 0.1% PEG 8000; 5 mM CaCl2; 185 mM Na+3.384.50.75pH 7.4PMID 15913649Open
factor IXa (FIXa)Homo sapienshuman coagulation factor XFX activation without FVIIIa: 20 nM FIXa1.17 × 10⁻⁴1.24 × 10⁻⁴0.940 calc.pH 7.5, 37 °CPMID 15913649Open
factor IXa (FIXa)Homo sapienshuman coagulation factor XFX activation with saturating FVIIIa: 0.05 nM FIXa1.758.5 × 10⁻⁶2.1 × 10⁵ calc.pH 7.5, 37 °CPMID 15913649Open
factor IXa (FIXa)variant Y225PHomo sapiensCH3SO2-D-Leu-Gly-Arg-p-nitroanilide1 mM EDTA; 200 mM Rb+ to keep ionic strength constant0.0052.10.0017pH 7.4PMID 15913649Open
factor IXa (FIXa)variant Y225PHomo sapiensCH3SO2-D-Leu-Gly-Arg-p-nitroanilide1 mM EDTA; 200 mM Na+; no Ca2+0.0583.10.018pH 7.4PMID 15913649Open
factor IXa (FIXa)variant Y225PHomo sapiensCH3SO2-D-Leu-Gly-Arg-p-nitroanilide5 mM CaCl2; 185 mM Rb+ to keep ionic strength constant0.8877.90.113pH 7.4PMID 15913649Open
factor IXa (FIXa)variant Y225PHomo sapiensCH3SO2-D-Leu-Gly-Arg-p-nitroanilide5 mM CaCl2; 185 mM Na+0.7837.30.107pH 7.4PMID 15913649Open
factor IXa (FIXa)variant Y225PHomo sapienshuman coagulation factor XFX activation without FVIIIa: 20 nM FIXa3.8 × 10⁻⁵1.26 × 10⁻⁴0.30 calc.pH 7.5, 37 °CPMID 15913649Open
factor IXa (FIXa)variant Y225PHomo sapienshuman coagulation factor XFX activation with saturating FVIIIa: 0.05 nM FIXa0.5578.2 × 10⁻⁶6.8 × 10⁴ calc.pH 7.5, 37 °CPMID 15913649Open