CatLogenzyme kinetics catalog

Values CatLog read from this paper

Factor IX mutants with enhanced catalytic activity

PMID 19656280doi:10.1111/j.1538-7836.2009.03560.xOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 18 records (42 values) from this paper: activated coagulation factor IX (FIXa) from Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.4.21.22

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
activated coagulation factor IX (FIXa)Homo sapiensH-D-Leu-Ph'Gly-Arg-pNA.2AcOHplasma-derived FIX activated with FXIa——1.05 × 10⁻⁴37 °COpen
activated coagulation factor IX (FIXa)Homo sapiensH-D-Leu-Ph'Gly-Arg-pNA.2AcOH——1.47 × 10⁻⁴37 °COpen
activated coagulation factor IX (FIXa)Homo sapienshuman coagulation factor XFX activation without FVIIIa in 25 mM Hepes; 6 nM FIXa2.12 × 10⁻⁴6.5 × 10⁻⁵3.2pH 7.35, 37 °COpen
activated coagulation factor IX (FIXa)Homo sapienshuman coagulation factor XFX activation with FVIIIa in 25 mM Hepes2.181.3 × 10⁻⁵1.7 × 10⁵pH 7.35, 37 °COpen
activated coagulation factor IX (FIXa)Homo sapienshuman coagulation factor XFX activation without FVIIIa in 25 mM Hepes; 6 nM FIXa2.87 × 10⁻⁴6.8 × 10⁻⁵4.2pH 7.35, 37 °COpen
activated coagulation factor IX (FIXa)Homo sapienshuman coagulation factor XFX activation with FVIIIa in 25 mM Hepes1.71.1 × 10⁻⁵1.5 × 10⁵pH 7.35, 37 °COpen
activated coagulation factor IX (FIXa)variant Y94F/A95aK/K98T/Y177F/I213V/E219G (FIXa-M)Homo sapiensH-D-Leu-Ph'Gly-Arg-pNA.2AcOH——1.23 × 10⁻⁴37 °COpen
activated coagulation factor IX (FIXa)variant Y94F/A95aK/K98T/Y177F/I213V/E219G (FIXa-M)Homo sapienshuman coagulation factor XFX activation without FVIIIa in 25 mM Hepes; 6 nM FIXa0.0010085.5 × 10⁻⁵18.2pH 7.35, 37 °COpen
activated coagulation factor IX (FIXa)variant Y94F/A95aK/K98T/Y177F/I213V/E219G (FIXa-M)Homo sapienshuman coagulation factor XFX activation with FVIIIa in 25 mM Hepes2.031.3 × 10⁻⁵1.5 × 10⁵pH 7.35, 37 °COpen
activated coagulation factor IX (FIXa)variant Y94F/K98T (FIXa-C)Homo sapiensH-D-Leu-Ph'Gly-Arg-pNA.2AcOH——3.7 × 10⁻⁴37 °COpen
activated coagulation factor IX (FIXa)variant Y94F/K98T (FIXa-C)Homo sapienshuman coagulation factor XFX activation without FVIIIa in 25 mM Hepes; 6 nM FIXa5.87 × 10⁻⁴8.3 × 10⁻⁵7.2pH 7.35, 37 °COpen
activated coagulation factor IX (FIXa)variant Y94F/K98T (FIXa-C)Homo sapienshuman coagulation factor XFX activation with FVIIIa in 25 mM Hepes31.5 × 10⁻⁵2 × 10⁵pH 7.35, 37 °COpen
activated coagulation factor IX (FIXa)variant Y94F/K98T/Y177F (FIXa-I)Homo sapiensH-D-Leu-Ph'Gly-Arg-pNA.2AcOH——1.05 × 10⁻⁴37 °COpen
activated coagulation factor IX (FIXa)variant Y94F/K98T/Y177F (FIXa-I)Homo sapienshuman coagulation factor XFX activation without FVIIIa in 25 mM Hepes; 6 nM FIXa3.3 × 10⁻⁴6.8 × 10⁻⁵4.8pH 7.35, 37 °COpen
activated coagulation factor IX (FIXa)variant Y94F/K98T/Y177F (FIXa-I)Homo sapienshuman coagulation factor XFX activation with FVIIIa in 25 mM Hepes1.131.4 × 10⁻⁵8.3 × 10⁴pH 7.35, 37 °COpen
activated coagulation factor IX (FIXa)variant Y94F/K98T/Y177F/I213V/E219G (FIXa-L)Homo sapiensH-D-Leu-Ph'Gly-Arg-pNA.2AcOH——2.53 × 10⁻⁴37 °COpen
activated coagulation factor IX (FIXa)variant Y94F/K98T/Y177F/I213V/E219G (FIXa-L)Homo sapienshuman coagulation factor XFX activation without FVIIIa in 25 mM Hepes; 6 nM FIXa0.002224 × 10⁻⁵55pH 7.35, 37 °COpen
activated coagulation factor IX (FIXa)variant Y94F/K98T/Y177F/I213V/E219G (FIXa-L)Homo sapienshuman coagulation factor XFX activation with FVIIIa in 25 mM Hepes8.81.7 × 10⁻⁵5.3 × 10⁵pH 7.35, 37 °COpen