CatLogenzyme kinetics catalog

Values CatLog read from this paper

Na+ site in blood coagulation factor IXa: effect on catalysis and factor VIIIa binding

PMID 15913649doi:10.1016/j.jmb.2005.04.052Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 16 records (36 values) from this paper: factor IXa (FIXa) from Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 4 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 3.4.21.22

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
factor IXa (FIXa)Homo sapiensCH3SO2-D-Leu-Gly-Arg-p-nitroanilide50 mM Tris-HCl; 0.1% (w/v) PEG 8000; Rb+ as compensating ion0.432——pH 7.4Open
factor IXa (FIXa)Homo sapiensCH3SO2-D-Leu-Gly-Arg-p-nitroanilide50 mM Tris-HCl; 0.1% (w/v) PEG 8000; Rb+ as compensating ion0.562——pH 7.4Open
factor IXa (FIXa)Homo sapiensCH3SO2-D-Leu-Gly-Arg-p-nitroanilide50 mM Tris-HCl; 0.1% (w/v) PEG 8000; Rb+ as compensating ion3.58——pH 7.4Open
factor IXa (FIXa)Homo sapiensCH3SO2-D-Leu-Gly-Arg-p-nitroanilide50 mM Tris-HCl; 0.1% (w/v) PEG 8000; Rb+ as compensating ion3.53——pH 7.4Open
factor IXa (FIXa)Homo sapiensCH3SO2-D-Leu-Gly-Arg-p-nitroanilide50 mM Tris (pH 7.4); 0.1% PEG 8000; 1 mM EDTA; no Na+0.421.90.22pH 7.4Open
factor IXa (FIXa)Homo sapiensCH3SO2-D-Leu-Gly-Arg-p-nitroanilide50 mM Tris (pH 7.4); 0.1% PEG 8000; 1 mM EDTA; 200 mM Na+0.630.51.22pH 7.4Open
factor IXa (FIXa)Homo sapiensCH3SO2-D-Leu-Gly-Arg-p-nitroanilide50 mM Tris (pH 7.4); 0.1% PEG 8000; 5 mM CaCl2; no Na+3.158.70.37pH 7.4Open
factor IXa (FIXa)Homo sapiensCH3SO2-D-Leu-Gly-Arg-p-nitroanilide50 mM Tris (pH 7.4); 0.1% PEG 8000; 5 mM CaCl2; 185 mM Na+3.384.50.75pH 7.4Open
factor IXa (FIXa)Homo sapienshuman coagulation factor XFX activation without FVIIIa: 20 nM FIXa1.17 × 10⁻⁴1.24 × 10⁻⁴0.940 calc.pH 7.5, 37 °COpen
factor IXa (FIXa)Homo sapienshuman coagulation factor XFX activation with saturating FVIIIa: 0.05 nM FIXa1.758.5 × 10⁻⁶2.1 × 10⁵ calc.pH 7.5, 37 °COpen
factor IXa (FIXa)variant Y225PHomo sapiensCH3SO2-D-Leu-Gly-Arg-p-nitroanilide1 mM EDTA; 200 mM Rb+ to keep ionic strength constant0.0052.10.0017pH 7.4Open
factor IXa (FIXa)variant Y225PHomo sapiensCH3SO2-D-Leu-Gly-Arg-p-nitroanilide1 mM EDTA; 200 mM Na+; no Ca2+0.0583.10.018pH 7.4Open
factor IXa (FIXa)variant Y225PHomo sapiensCH3SO2-D-Leu-Gly-Arg-p-nitroanilide5 mM CaCl2; 185 mM Rb+ to keep ionic strength constant0.8877.90.113pH 7.4Open
factor IXa (FIXa)variant Y225PHomo sapiensCH3SO2-D-Leu-Gly-Arg-p-nitroanilide5 mM CaCl2; 185 mM Na+0.7837.30.107pH 7.4Open
factor IXa (FIXa)variant Y225PHomo sapienshuman coagulation factor XFX activation without FVIIIa: 20 nM FIXa3.8 × 10⁻⁵1.26 × 10⁻⁴0.30 calc.pH 7.5, 37 °COpen
factor IXa (FIXa)variant Y225PHomo sapienshuman coagulation factor XFX activation with saturating FVIIIa: 0.05 nM FIXa0.5578.2 × 10⁻⁶6.8 × 10⁴ calc.pH 7.5, 37 °COpen